Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,460,8710TG43.1% 122.5 / 11.3 72Y597S (TAC→TCC) nrdDanaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base T (9/32);  new base G (31/0);  total (40/32)
Fisher's exact test for biased strand distribution p-value = 1.25e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTT  >  NC_000913/4460730‑4461021
                                                                                                                                             |                                                                                                                                                      
cACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGt                                                                                                                                                        >  1:1290645/1‑142 (MQ=255)
     atcaATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCa                                                                                                                                             <  1:868442/148‑1 (MQ=255)
     atcaATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAg                                                                                                                                          <  2:1462478/151‑1 (MQ=255)
        aaTCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAGTCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCg                                                                                                                                       <  2:583256/151‑1 (MQ=255)
        aaTCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCg                                                                                                                                       <  2:321410/151‑1 (MQ=255)
        aaTCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCg                                                                                                                                       <  1:1209489/151‑1 (MQ=255)
        aaTCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAAGCGCTTcca                                                                                                                                            >  2:1329078/1‑144 (MQ=255)
           cGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGAt                                                                                                                                     <  2:152892/150‑1 (MQ=255)
            ggTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGCTTTAAAAACGATCt                                                                                                                                   >  2:1308275/1‑151 (MQ=255)
            ggTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGCTTCAAAATCGATCt                                                                                                                                   >  2:1417697/1‑151 (MQ=255)
               gtATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGCTTTCAAAACGATCtt                                                                                                                                  >  2:800628/1‑149 (MQ=255)
               gtATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCCTCCAAATCGATCTTg                                                                                                                                 >  1:1073078/1‑150 (MQ=255)
                tATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGCGTCCAAGTCGGTCtt                                                                                                                                  >  1:513913/1‑148 (MQ=255)
                        ccGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACggg                                                                                                                        <  1:1450619/150‑1 (MQ=255)
                        ccGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCTTTCAAGACGATCTTGTCGTACggg                                                                                                                        >  1:1180867/1‑150 (MQ=255)
                         cGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGtt                                                                                                                      <  1:768530/151‑1 (MQ=255)
                          gTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAAGGGCCTCAAAAACGATCTTGTCGTACggggt                                                                                                                      >  2:1180016/1‑148 (MQ=255)
                               aCGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAAGGGCGTCAAAAAAGGTATTTTCGTACGGGTGTACctt                                                                                                                >  1:281777/1‑151 (MQ=255)
                                cGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAAGGGCTTCCAAAACGATCTTTTCGTACGGGGTCAActt                                                                                                                >  1:970311/1‑150 (MQ=255)
                                 ggTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACcttct                                                                                                              <  2:1071423/151‑1 (MQ=255)
                                  gTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACcttct                                                                                                              <  1:1152930/150‑1 (MQ=255)
                                    aCATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCttcttc                                                                                                            <  1:236116/150‑1 (MQ=255)
                                       tGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTTATGGGCGTCAAAGGCGGTTTTTTCGTTTGGGTTCAcccttt                                                                                                              >  1:247256/1‑141 (MQ=255)
                                           gATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCcaca                                                                                                      <  1:853847/149‑1 (MQ=255)
                                            aTAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCATCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCg                                                                                                   <  1:67907/151‑1 (MQ=255)
                                             tAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGGAGGCGGTTCGAAAAAGACTTTGCCGGACGGGGTCACCAtcctct                                                                                                           >  2:362964/1‑142 (MQ=255)
                                             tAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCCTCAAAATCGGTCTTTTTGTTCCGGGTTACCCtcttcttc                                                                                                         >  2:628982/1‑142 (MQ=255)
                                                 tGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGGTTCAAAATCGATCTTGTCGTAAGGGGTCAACCTCTTTCCCCCATCCAgggg                                                                                              >  1:1424659/1‑149 (MQ=255)
                                                 tGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAAGGGCCTCAAAAACGATTTTTTTGTTCGGGGTCACCCTCTTCTTCACAACCAgggg                                                                                              >  1:1440077/1‑149 (MQ=255)
                                                  gTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTg                                                                                              <  1:1180892/150‑1 (MQ=255)
                                                    aaTCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGa                                                                                            <  2:944183/150‑1 (MQ=255)
                                                      tCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCGGCAAAGTCGATATTTTCGGACGGGTGCCCCTTCTTCTCCCCATCGGGGTGGgaa                                                                                          >  2:608783/1‑150 (MQ=255)
                                                       cccAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACt                                                                                        <  2:1281741/151‑1 (MQ=255)
                                                       cccAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAAGGGCTTCAAAGTCCATCTTTTTGGACGGGGTCACCCTCTTCTCCACCTCGAGGGGGaaa                                                                                          >  2:100382/1‑149 (MQ=255)
                                                         cAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGt                                                                                      <  1:131825/151‑1 (MQ=255)
                                                          aGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGtt                                                                                     <  2:1182128/151‑1 (MQ=255)
                                                          aGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAAGGGCTTCAAAGTCGATCTTGTCGTACGGGGTCTCCTTCTTCTCCCCATCGCGgggggga                                                                                          >  1:889594/1‑143 (MQ=255)
                                                              aTCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGGAAGGGCCTCAAAGTTGAGCTTGTCGGACAGGGTCACACTCCTTTCCTCACCGAGGTGGGAACTgat                                                                                     >  1:102621/1‑145 (MQ=255)
                                                                 ttCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGCGTCAAAGACGGTCTTTTCGTTCGGGGTCACCTTTTTTTCCCCAACGAGGGGGGAAATGTTtgtg                                                                                 >  1:861257/1‑148 (MQ=255)
                                                                  tCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTgta                                                                               <  2:403694/149‑1 (MQ=255)
                                                                   ccAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGGGCTTCAAAGTCGCTCTTTTCGTATGGGGTCACCCTCTTCTCCCCATCCGGGGGGGAAATGTTTGGgtag                                                                              >  2:298413/1‑149 (MQ=255)
                                                                   ccAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGGGCCTCAAAATCGATCTTTTCGTACGGGGTCACCCTCTTCTCCCCATCCAGGGGGGAACTGTTGgtgt                                                                                >  1:409852/1‑147 (MQ=255)
                                                                   ccAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCCTCCAAGACGAACTTTTCGTACGGGGTCACCTTCTTTTCCCCATCCAAGGGGGAACTGGTGGGgtagt                                                                             >  1:1539522/1‑150 (MQ=255)
                                                                     aGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGGAGGCGCTTCCAAATCGATCTTGTTGTACGGGGTCACCTTCTTCTCCCCATCCAGGGGGGAAATGTTTGTGTAGTaa                                                                           >  1:702122/1‑150 (MQ=255)
                                                                       cgcCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCTTCAAAATCGATCTTTTCGTACGGGGTCACCTTCTTCTCCACATCCAGGGGGGAACTGTTTGGGTAGTAAcc                                                                         >  2:1554621/1‑150 (MQ=255)
                                                                         ccTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAATCGAACTTTTCCTAAGGGGTCACCTTTTTCTCCCCACCGGGGGGGGAAATGTTGGGGTAGTAACCttt                                                                      >  2:1138591/1‑151 (MQ=255)
                                                                            tCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTAt                                                                    <  2:94470/150‑1 (MQ=255)
                                                                            tCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTAt                                                                    <  2:1031057/150‑1 (MQ=255)
                                                                            tCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTAt                                                                    <  1:427584/150‑1 (MQ=255)
                                                                            tCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAAGGGCTTCAAAAACGAACTTTTCGTACGGGGTCACCCTCTTCTCCACCTCGAGGGGGGAAATGTTGGTGTAGTAAACTTTAt                                                                    >  2:1074652/1‑150 (MQ=255)
                                                                               ggTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCTTCCCAATCGATCTTTTCGTACGGGGTCACCTTCTTTTCCACATCCAGGGGGGAAATGTTGGTGTAGTAACCttttt                                                                    >  1:295782/1‑145 (MQ=255)
                                                                                 ttGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGCTTCAAAAAGGGTATTGTCGTTCGGGGTCACCTTCTTTTCCCCACCGAGGGGGAAAATGTTGGGGTAGTAAACttttt                                                                    >  1:1525656/1‑143 (MQ=255)
                                                                                 ttGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCCTCAAAATCGAACTTTTCGTACGGGGTTCCCCTCTTCTCCCCATCGAGGGGGGAACTGTTGGTGTAGTAAACttttttgg                                                                 >  2:1311632/1‑143 (MQ=255)
                                                                                  tgtgCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAAGCGCCTCCAAAACGATCTTTTCGTACGGGGTCACCTTCTTTTCCCCATCCCGGGGGGAAACGTTTGGGTAGTTAACTTTTTCTGTccc                                                             >  1:273173/1‑149 (MQ=255)
                                                                                   gtgCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCAc                                                             <  2:325985/150‑1 (MQ=255)
                                                                                     gCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGc                                                           <  1:1216476/150‑1 (MQ=255)
                                                                                     gCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGGCTCTCAAACGAACTTTTTGTACGGGGTCCCCTTCTTCTTCACACCCAGGGGGGAAATGGTGGGGTGGTATCCttttt                                                                    >  1:180599/1‑139 (MQ=255)
                                                                                        gAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCg                                                        <  2:1179716/150‑1 (MQ=255)
                                                                                         aaTGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCCTCAAAATCGATCTTTTCGTACGGGGTTACCCTCTTTTCCCCAACGAGGGGGGAAATGTTGGGGGTGTAAACCTTATCGGTCACGccccg                                                       >  2:819178/1‑148 (MQ=255)
                                                                                              ttGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGcacca                                                  <  2:817270/150‑1 (MQ=255)
                                                                                               tGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGcaccac                                                 <  1:1545955/150‑1 (MQ=255)
                                                                                                    aCTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAAGCGCTTCCGAGACGATCTTTTCGGTCGGGTTCACCTTCTTTTCCCCACCGAGGGGGGAACTGTTGGGGGAGTAACCTTTATCCGTCCCGCCCCGCACCACGcc                                              >  1:87853/1‑148 (MQ=255)
                                                                                                         ccGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGGAAGCGCGTCAAAAAAGAACTTGTTGTTCGGGGTCACCCTCTTTTCCCCAACGAGGGGGGAACAGTTGGTGTGGTAACCTTTTTCTGTCCCCCCCCGCACCCCGcc                                              >  1:291585/1‑143 (MQ=255)
                                                                                                              gCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTa                                 <  2:494655/151‑1 (MQ=255)
                                                                                                               cAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTAt                                <  2:163757/151‑1 (MQ=255)
                                                                                                                   tGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGCTTCAAAGTTGATCCTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGGGGAAAATGTTGGGGTAGTAAACTTTTTCGGTCACGCCCCGCACCACGCCAAACTCAGCAGGATCg                              >  1:262607/1‑149 (MQ=255)
                                                                                                                           ccGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAAGCGAACTTGTTGTACGGGGTCACCTTTTTTTCCCCAACGAGGGGGAAAATGTTGGTGTAGTAACCTTTATTGGGCACGCCCGGCCCCACCCCAAAACCAGCACTAt                                >  2:211654/1‑139 (MQ=255)
                                                                                                                               tCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGc                 <  2:1038889/150‑1 (MQ=255)
                                                                                                                                cGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGa               <  2:289693/151‑1 (MQ=255)
                                                                                                                                cGCCAGCGGCGGGGAAGGGGCTTCAAAATCGATCTTTTTGTACGGGGTCACCTTCTTCTCCACATCGAGGGGGGAACTGTTGGGGTAGTAACCCTTTTCGGTCACCCCCCGCCCCCCGCCAAACTCAGCAGTATCGAGACGGCAGaa                   >  1:719220/1‑147 (MQ=255)
                                                                                                                                 gCCAGCGGCGGGGAAGGGGCCTCAAAGGTGGTCTTTTCGGACGGGGTCCCCCTTTTTTCCCCCTCGAGGGGGGAAATGTTGGTGTAGTAACCTTTATCTGTCCCCCCCCGCCCCCCCCCAAAATCAGCCGGATCGGGACGGGAGaa                   >  1:975994/1‑146 (MQ=255)
                                                                                                                                       ggcggGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGcaca        <  1:527276/151‑1 (MQ=255)
                                                                                                                                           ggTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGtt    >  1:1166966/1‑151 (MQ=255)
                                                                                                                                             tAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGtttt  >  2:264166/1‑151 (MQ=255)
                                                                                                                                             |                                                                                                                                                      
CACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTT  >  NC_000913/4460730‑4461021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: