Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 576,891 C→T intergenic (‑66/‑507) nmpC ← / → essD DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage/DLP12 prophage; putative phage lysis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913576,8910CT99.0% 379.2 / ‑4.0 100intergenic (‑66/‑507)nmpC/essDDLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage/DLP12 prophage; putative phage lysis protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (52/47);  total (52/48)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 101 total aligned reads displayed.

TATAAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATCAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACG  >  NC_000913/576744‑577037
                                                                                                                                                   |                                                                                                                                                  
tataAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTaa                                                                                                                                                  <  2:552296/150‑1 (MQ=255)
tataAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTaa                                                                                                                                                  <  1:766016/150‑1 (MQ=255)
 ataAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCtaattaat                                                                                                                                                 <  1:216762/150‑1 (MQ=255)
  taAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAAtg                                                                                                                                                >  1:586889/1‑150 (MQ=255)
         cAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGattatt                                                                                                                                        >  2:1394468/1‑151 (MQ=255)
          aGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTAttt                                                                                                                                       <  2:251002/151‑1 (MQ=255)
           gCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTGCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGatta                                                                                                                                          <  1:808441/147‑1 (MQ=255)
              gCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCa                                                                                                                                     <  2:1347192/149‑1 (MQ=255)
                 tGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATg                                                                                                                                >  1:803221/1‑151 (MQ=255)
                   aGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTa                                                                                                                              <  2:142144/151‑1 (MQ=255)
                    gCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTa                                                                                                                              >  1:352831/1‑150 (MQ=255)
                    gCAGACATCGCAATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTa                                                                                                                              >  1:1528219/1‑150 (MQ=255)
                       gACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGtt                                                                                                                          <  2:967647/151‑1 (MQ=255)
                       gACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGtt                                                                                                                          <  1:119915/151‑1 (MQ=255)
                            cGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTgg                                                                                                                      <  2:724163/150‑1 (MQ=255)
                               cATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTtc                                                                                                                  >  2:1328034/1‑151 (MQ=255)
                                   aGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCg                                                                                                               <  1:1011676/150‑1 (MQ=255)
                                       cTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTg                                                                                                           >  1:204058/1‑150 (MQ=255)
                                        tGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGAt                                                                                                         >  2:643878/1‑151 (MQ=255)
                                        tGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGAt                                                                                                         >  1:817550/1‑151 (MQ=255)
                                         gATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGa                                                                                                          <  1:80655/149‑1 (MQ=255)
                                         gATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGAt                                                                                                         <  2:1210887/150‑1 (MQ=255)
                                          aTGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGAttt                                                                                                       <  2:1386427/151‑1 (MQ=255)
                                             cAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTtgtg                                                                                                    >  1:495811/1‑151 (MQ=255)
                                                 tACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAg                                                                                                 >  1:459173/1‑150 (MQ=255)
                                                  aCAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGt                                                                                                <  1:1409850/150‑1 (MQ=255)
                                                  aCAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTa                                                                                               <  1:394962/151‑1 (MQ=255)
                                                     gcagAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGAt                                                                                            <  1:493457/151‑1 (MQ=255)
                                                      cagAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGt                                                                                                >  1:117249/1‑146 (MQ=255)
                                                         aaaTTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATctct                                                                                        >  1:972638/1‑151 (MQ=255)
                                                          aaTTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTa                                                                                       <  2:161851/151‑1 (MQ=255)
                                                            ttGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTAtt                                                                                     >  2:14185/1‑151 (MQ=255)
                                                             tGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTAttt                                                                                    >  1:906091/1‑151 (MQ=255)
                                                              gCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTAttt                                                                                    <  2:763442/150‑1 (MQ=255)
                                                              gCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTAttt                                                                                    <  1:92138/150‑1 (MQ=255)
                                                               ccACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTAttttt                                                                                  <  1:177211/151‑1 (MQ=255)
                                                                cACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTa                                                                                 <  2:417416/151‑1 (MQ=255)
                                                                cACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTa                                                                                 <  2:178447/151‑1 (MQ=255)
                                                                 aCTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTAt                                                                                >  1:430576/1‑151 (MQ=255)
                                                                    gTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTg                                                                             >  1:1250543/1‑151 (MQ=255)
                                                                       aaTTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAAc                                                                          <  1:705476/151‑1 (MQ=255)
                                                                         ttttttCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGAGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACtt                                                                        >  1:1204952/1‑151 (MQ=255)
                                                                          tttttCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACttt                                                                       >  2:1218477/1‑151 (MQ=255)
                                                                            tttCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACttttt                                                                     >  2:649765/1‑151 (MQ=255)
                                                                            tttCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACttttt                                                                     >  2:1154220/1‑151 (MQ=255)
                                                                            tttCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACtttt                                                                      <  1:492591/150‑1 (MQ=255)
                                                                               cATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCt                                                                  >  2:675039/1‑151 (MQ=255)
                                                                               cATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCt                                                                  >  2:886435/1‑151 (MQ=255)
                                                                               cATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCt                                                                  >  1:432501/1‑151 (MQ=255)
                                                                               cATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCt                                                                  <  1:306040/151‑1 (MQ=255)
                                                                                     gAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGaa                                                            <  1:1029916/151‑1 (MQ=255)
                                                                                     gAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGa                                                             <  2:1101139/150‑1 (MQ=255)
                                                                                      aGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAAt                                                           >  2:485729/1‑151 (MQ=255)
                                                                                      aGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAAt                                                           <  1:1237991/151‑1 (MQ=255)
                                                                                      aGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAAt                                                           >  1:1480829/1‑151 (MQ=255)
                                                                                       gCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATc                                                          <  2:612784/151‑1 (MQ=255)
                                                                                         ccTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCt                                                        >  2:22555/1‑151 (MQ=255)
                                                                                          cTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCt                                                        >  1:1450360/1‑150 (MQ=255)
                                                                                             tttttGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTAtt                                                     <  1:721489/150‑1 (MQ=255)
                                                                                             tttttGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTc                                                    >  2:103548/1‑151 (MQ=255)
                                                                                                ttGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATg                                                 >  1:927657/1‑151 (MQ=255)
                                                                                                   aaCTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGct                                              >  1:1348082/1‑151 (MQ=255)
                                                                                                    aCTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGctc                                             >  1:275017/1‑151 (MQ=255)
                                                                                                     cTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGctc                                             <  1:1233751/150‑1 (MQ=255)
                                                                                                       attattAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCtt                                           <  1:1217298/150‑1 (MQ=255)
                                                                                                       attattAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCtt                                           <  1:482672/150‑1 (MQ=255)
                                                                                                          attaAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCt                                       <  2:1133627/151‑1 (MQ=255)
                                                                                                          attaAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCt                                       >  2:796718/1‑151 (MQ=255)
                                                                                                          attaAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCt                                       >  2:765428/1‑151 (MQ=255)
                                                                                                            taAAAAATGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTg                                      >  2:126042/1‑150 (MQ=255)
                                                                                                             aaaaaaTGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGa                                     <  1:516143/150‑1 (MQ=255)
                                                                                                             aaaaaaTGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGa                                     >  2:670327/1‑150 (MQ=255)
                                                                                                             aaaaaaTGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGa                                     >  1:597331/1‑150 (MQ=255)
                                                                                                               aaaaTGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGaata                                  <  2:354850/151‑1 (MQ=255)
                                                                                                               aaaaTGATGTCACTGCGCGATAAATATTCATCTAATCAATGTTATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGaata                                  <  2:677999/151‑1 (MQ=255)
                                                                                                                aaaTGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGaata                                  >  2:1000568/1‑150 (MQ=255)
                                                                                                                 aaTGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGaataaa                                <  1:1230925/151‑1 (MQ=255)
                                                                                                                 aaTGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGaataaa                                <  2:168169/151‑1 (MQ=255)
                                                                                                                 aaTGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGaataa                                 >  2:1522384/1‑150 (MQ=255)
                                                                                                                 aaTGATGTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGaataa                                 >  1:343152/1‑150 (MQ=255)
                                                                                                                  atgatgTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGaataaaa                               <  2:297341/151‑1 (MQ=255)
                                                                                                                  atgatgTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGaataaaa                               <  1:1506402/151‑1 (MQ=255)
                                                                                                                  atgatgTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGaataa                                 >  1:543912/1‑149 (MQ=255)
                                                                                                                       gTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGaataaaaataa                           <  1:711639/150‑1 (MQ=255)
                                                                                                                       gTCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGaataaaaataa                           <  1:246279/150‑1 (MQ=255)
                                                                                                                        tCACTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAaaataaat                         >  2:831674/1‑151 (MQ=255)
                                                                                                                          aCTGCGCGATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCt                       >  2:1010453/1‑151 (MQ=255)
                                                                                                                              cgcgATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTAtt                    >  1:188510/1‑150 (MQ=255)
                                                                                                                                cgATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGc                 >  2:1211429/1‑151 (MQ=255)
                                                                                                                                 gATAAATATTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGcc                >  2:882680/1‑151 (MQ=255)
                                                                                                                                      atatTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGACTCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAAttt           >  2:1534736/1‑151 (MQ=255)
                                                                                                                                      atatTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAAttt           <  1:466951/151‑1 (MQ=255)
                                                                                                                                        atTCATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTtat         >  1:529642/1‑151 (MQ=255)
                                                                                                                                           cATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTatat       <  2:665367/150‑1 (MQ=255)
                                                                                                                                           cATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTatat       <  1:244152/150‑1 (MQ=255)
                                                                                                                                           cATCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATAtt      >  1:959571/1‑151 (MQ=255)
                                                                                                                                             tCTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTa     >  1:1435468/1‑150 (MQ=255)
                                                                                                                                              cTAATTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAAc   >  1:433965/1‑151 (MQ=255)
                                                                                                                                                aaTTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTa     <  1:1530078/147‑1 (MQ=255)
                                                                                                                                                aaTTAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACg  <  1:1460901/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATAAATTTCAGCTGCCTGAGCAGACATCGCCATTAGTACTGATGCAGCTACAGCAGAAATTGCCACTGTTAATTTTTTCATCGTGAGCCCTTTTTTTGAACTATTATTAAAAAATGATGTCACTGCGCGATAAATATTCATCTAATCAATGTGATTATTTCAAGATGTAAGTTTTGGTTTCTCGTTTGATTTGTGAAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACG  >  NC_000913/576744‑577037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: