Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,234	0	G	T	45.2%	85.0 / 50.0	73	L25F (TTG→TTT)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (8/32);  new base T (33/0);  total (41/32)
Fisher's exact test for biased strand distribution p-value = 1.60e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATT  >  NC_000913/1245091‑1245384                                                                                                                                                |                                                                                                                                                          agccGATAAAGTCGGTTCCCAGTAACAACAACAGAATCCTTGTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGCCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTga                                                                                                                                                          <  1:116553/143‑2 (MQ=255)   ccGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCtt                                                                                                                                                  <  2:715897/151‑1 (MQ=255)    cGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTa                                                                                                                                                 <  2:1145346/151‑1 (MQ=255)    cGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTa                                                                                                                                                 <  2:843603/151‑1 (MQ=255)    cGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTGTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTa                                                                                                                                                 <  2:811735/151‑1 (MQ=255)           gTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACa                                                                                                                                          <  2:1194008/151‑1 (MQ=255)               gTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTa                                                                                                                                      <  1:248239/151‑1 (MQ=255)                ttCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTTTTTAAAAttt                                                                                                                                       >  2:1267097/1‑149 (MQ=255)                 tCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTTAACAttt                                                                                                                                       >  2:162941/1‑148 (MQ=255)                    cGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGATGCGGTTATTTaaaaatttaaaat                                                                                                                                 >  1:370076/1‑140 (MQ=255)                     ggTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTg                                                                                                                                <  2:940938/151‑1 (MQ=255)                        aacaacaacAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGGGCTTTTTTAACATTTAGaaat                                                                                                                                 >  1:188690/1‑145 (MQ=255)                         acaacaacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑Ca                                                                                                                           <  1:1257402/151‑1 (MQ=255)                                  gAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTg                                                                                                                  <  1:843850/151‑1 (MQ=255)                                    aTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTGAACATTTTGAATTTAT‑TAATCCTTCt                                                                                                                   >  2:599100/1‑148 (MQ=255)                                    aTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCCTATTTAACATTTAGAATTTAT‑CAATCCtt                                                                                                                     >  2:817839/1‑146 (MQ=255)                                           tttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTGGACGTGATTATTATAGATATAGGAGTGAG‑CTATTCTTCTGGCGGa                                                                                                             >  2:260245/1‑147 (MQ=255)                                            ttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAACAATTAGAATTTTA‑CAAACCTTCTTGCAGA‑TTa                                                                                                         >  2:917399/1‑149 (MQ=255)                                             tAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAAAATTTATAATTGAT‑CAAACCTTCTGGCAGaatta                                                                                                         >  1:1199168/1‑145 (MQ=255)                                             tAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGGTGGGCTTATTGAAAATTTAGAAATGATGAAATCCTT‑TGGCAGA‑tt                                                                                                          >  2:756426/1‑147 (MQ=255)                                             tAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTTTTTAACATTTTGAAATTAT‑CAATCCTTCTGGCAGA‑TTaa                                                                                                        >  2:1059166/1‑149 (MQ=255)                                             tAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTTGGTGCGGCTTTTTTACATTTTGAATTTAT‑CAATACtttttggaggtt                                                                                                           >  1:892445/1‑137 (MQ=255)                                              aaTTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAACAATTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAAcc                                                                                                     >  2:517672/1‑151 (MQ=255)                                               attattGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTTTTTAAAAATTTGGATTGAT‑CAAACCTTTTGGGCGA‑TTAAGcc                                                                                                     >  1:288737/1‑150 (MQ=255)                                                  attGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAAAATTTAGAATTGAT‑CAAAACCTCTGGGAGA‑TTAAGCCaaa                                                                                                  >  1:1290628/1‑150 (MQ=255)                                                  attGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCaaa                                                                                                  <  2:1168547/150‑1 (MQ=255)                                                   ttGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTTTTTAACATTTAGAATTTAT‑TAAAACTTCTGGCAGA‑TTAAGCCaaaa                                                                                                 >  1:696168/1‑150 (MQ=255)                                                    tGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGTTGGGCTTTTTTAAAATTTTGAATTTAT‑TAAACCTTCTGGGAGA‑TTAAACCcaaa                                                                                                 >  1:770961/1‑149 (MQ=255)                                                    tGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTTAACATTTAAAAATGAT‑CAATCCTTCTGGCAGA‑TTAAACCaaaa                                                                                                 >  2:1080680/1‑149 (MQ=255)                                                     gTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATa                                                                                               <  1:1077120/150‑1 (MQ=255)                                                            tgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAAAATTTTTAATTTAT‑CAATCCCTCTGGCAGA‑TTAAGCCAAAAAAC‑CCGtt                                                                                        >  1:262804/1‑149 (MQ=255)                                                                 ttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTTAAAATTTAGAAATTAT‑CAAACCTTTTGGCAGA‑TTAAGCCAAAATAA‑CCGTTCgg                                                                                     >  1:1147927/1‑147 (MQ=255)                                                                      gtgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCtt                                                                             <  2:313700/150‑1 (MQ=255)                                                                       tgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAACATTTAAAAATGAT‑CAATCCTTCTGGCAGA‑ATAAACCAAAATAA‑CCGGTCGGCAAAGCtt                                                                             >  2:600735/1‑149 (MQ=255)                                                                         aTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGt                                                                         <  1:453248/151‑1 (MQ=255)                                                                            cAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCCTATTTAACATTTAAAATTGAT‑CAATCCTTCTCGCAGA‑TTAAGCCAAAAAAA‑CCCGTCGGGAAAGCTTCAGtt                                                                        >  2:564231/1‑149 (MQ=255)                                                                            cAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTa                                                                       <  1:949521/150‑1 (MQ=255)                                                                            cAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTAc                                                                      <  1:282425/151‑1 (MQ=255)                                                                              gAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTAcgc                                                                    <  1:1109221/151‑1 (MQ=255)                                                                                aaataaatATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGa                                                                  <  2:1180809/151‑1 (MQ=255)                                                                                 aataaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAAAATTTTGAATTGAT‑CAATCCTTCTGGCAGA‑TTTAGCCAAAAAAC‑CCGTTCGGCAATGCTTCCGTTAcgcg                                                                   >  2:732917/1‑149 (MQ=255)                                                                                   taaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAAAAATTTGAATTGAT‑CAAACCTTCTGGGCGA‑TTAAGGCCAAATAC‑CCGTTCCGCCAAGCTTTAGtt                                                                        >  1:52150/1‑142 (MQ=255)                                                                                     aataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAAAATTTAGAATTTAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAAAAC‑CCCGTCGGCAATGCTTCAGTTACGCGAAGAgg                                                             >  2:1100781/1‑151 (MQ=255)                                                                                       tatTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTTAAAAATTAGAATTGAT‑CAAACCTTCTGGCAGA‑TTTAACCAAAATAA‑CCGTTCGGCAAAGCTTCCGTTAcgc                                                                    >  1:407880/1‑142 (MQ=255)                                                                                        atTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAACATTTTGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAACCAAAAAAC‑CCGTTCGGCAAAGCTTCCGTTACCCGAAGAggga                                                           >  2:116218/1‑148 (MQ=255)                                                                                        atTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAAt                                                          <  2:1183898/151‑1 (MQ=255)                                                                                          tttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATg                                                         <  1:247088/150‑1 (MQ=255)                                                                                           ttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTTAAATTTTGAATTGTT‑CAAATCTTCTTGCAGATTTAAGCCAAAAAAC‑CCTTTC‑GCAATGCTTTAATTAcggcgagaaggaagg                                                        >  1:891153/1‑137 (MQ=255)                                                                                               aCAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACAGTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGa                                                   <  2:740476/151‑1 (MQ=255)                                                                                                cAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGaa                                                  <  1:661083/151‑1 (MQ=255)                                                                                                cAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGATTTTGGTGCGCCTATTGGACATTTAAAAATGGT‑CTATTCTTCTTGCCGA‑TTAAGCCAAAAAACACCATTGGGCAA‑GCTTTCATTTCCCGAAGAgg                                                             >  2:940923/1‑140 (MQ=255)                                                                                                    aGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTc                                               <  1:1135379/150‑1 (MQ=255)                                                                                                     gCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCtt                                             <  1:1018119/151‑1 (MQ=255)                                                                                                     gCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCtt                                             <  2:1218015/151‑1 (MQ=255)                                                                                                        cgTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAAAATATTGAATTTAT‑AAATTCCTTTGGCAGA‑TTAAGCCAAAATAA‑CCCTTCTGCAAAGATTCAGTTATGCGGAGAGGGAAGGTCTGAATTCttttt                                          >  1:1108355/2‑151 (MQ=255)                                                                                                        aGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTTTTTAACATTTAGAAATTAT‑TAAAACTTCTGGGAGA‑TTAAACCAAAATAA‑CCCTTCGGCAATGCTTCAGTTACGCGGAGAGGGAAGGGTTGAATTCttttt                                          >  1:375742/1‑151 (MQ=255)                                                                                                         gTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCtttttt                                         <  2:1072862/151‑1 (MQ=255)                                                                                                           cAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGCGCGCTTATTTAACATTTAGAATTTAT‑CAAACCTTCTTGGAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGGCTGAATTCttttttt                                        >  1:1082552/1‑150 (MQ=255)                                                                                                                  tAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAAAATTTAGAATTGAT‑TAAACCTTCTGGGAGA‑TTAAGGCCAAATAA‑CCGTTCGGCAATGGTTCAGTTTCGCGGAGAGGGATGGTCTGAATTTTTTTTTTTGtt                                    >  1:278186/1‑147 (MQ=255)                                                                                                                     aaaTGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCCTTTTGAACAATTTTAATTGAT‑CAATCCTTCTGGCAGA‑TTTAGCCAAAAAAC‑CCGTTCGGCCATGCTTTAGTTACGCGAAGAGGAATGGTCTTAATTCTTTTTTTTGTTGCTaaa                              >  1:279838/1‑150 (MQ=255)                                                                                                                      aaTGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAACAATTAGAAATGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAAAAA‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTTGGTGCTaaa                              >  2:282126/1‑149 (MQ=255)                                                                                                                      aaTGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAAAATTTTGAAATTAT‑CAATACTTCTTGCAGA‑TTAAACCAAAAAAC‑CCCTTCGGCAATTGTTCAGTTTCGCGAAGAGGGATGGTCTGAATTTTTTTTTTTGGTTCTAAAtt                            >  1:685566/1‑151 (MQ=255)                                                                                                                      aaTGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAACATTTTGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAAAAA‑CCCTTTGGCAATGCTTCAGTTACGCGAAGAGGAAAGGTCTGAATTCTTTTTTTTGGTGTTaaa                              >  2:1117090/1‑149 (MQ=255)                                                                                                                          gggCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTctct                        <  2:188711/151‑1 (MQ=255)                                                                                                                             cGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGa                     <  2:1100167/151‑1 (MQ=255)                                                                                                                              ggATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAg                    <  2:1222390/151‑1 (MQ=255)                                                                                                                               gATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAgt                   <  1:117777/151‑1 (MQ=255)                                                                                                                                  aGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAgtgt                 <  1:1227444/150‑1 (MQ=255)                                                                                                                                    tGAGGCGGTTTTTGCTGCGCTTATTGTACATTTTTAATTGAT‑TAATCCTTCTGGCAGA‑TTAAGCCCAAAAAC‑CCGTTCGGCAATGCTTTTGTTTCGCGAAGAGGGATGGTCTGAATTTTTTTTTTGGTTGCTAAATTTTCTTGGGTGTCt               >  1:441787/1‑150 (MQ=255)                                                                                                                                       ggCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGAt           <  2:44007/151‑1 (MQ=255)                                                                                                                                          ggTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAt        <  2:59790/151‑1 (MQ=255)                                                                                                                                             tttGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtgtg     >  1:672827/1‑151 (MQ=255)                                                                                                                                              ttGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtgtg     >  2:1063588/1‑150 (MQ=255)                                                                                                                                              ttGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtgtg     >  1:501022/1‑150 (MQ=255)                                                                                                                                                ggCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGatt  <  1:732862/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                          ATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAT‑CAATCCTTCTGGCAGA‑TTAAGCCAAAATAC‑CCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATT  >  NC_000913/1245091‑1245384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑