Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,679,3040AC43.1% 99.5 / 20.8 72Y293S (TAC→TCC) ydgHDUF1471 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (30/11);  new base C (0/31);  total (30/42)
Fisher's exact test for biased strand distribution p-value = 2.13e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGC  >  NC_000913/1679157‑1679451
                                                                                                                                                   |                                                                                                                                                   
aTGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTAcc                                                                                                                                                   >  2:182550/1‑150 (MQ=255)
    cACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGCTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATc                                                                                                                                              <  2:170129/151‑1 (MQ=255)
        aaaGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCAccc                                                                                                                                          >  2:1050658/1‑151 (MQ=255)
        aaaGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCAccc                                                                                                                                          <  1:510037/151‑1 (MQ=255)
                  aaCTAAAAAAAGCGACCGCGGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCAAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCa                                                                                                                                <  1:585448/151‑1 (MQ=255)
                     tGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAgg                                                                                                                              >  2:80143/1‑150 (MQ=255)
                       aaCAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAAc                                                                                                                           >  2:52328/1‑151 (MQ=255)
                         cAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACg                                                                                                                          >  1:519602/1‑150 (MQ=255)
                          aaaGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTg                                                                                                                        >  1:777723/1‑151 (MQ=255)
                                 ccGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCGGGCAACTCCGGCAACATGACCGAATTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGTGGtaa                                                                                                                    <  2:1068547/148‑1 (MQ=255)
                                  cGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAAc                                                                                                                >  2:231936/1‑151 (MQ=255)
                                      gCGATGATGGTCCCGTTCGACATCACAAAATTCTCGGGCAACTAGGGCAACATGCCCGAATTCTCCTATCAGGTTGCGAACCGTGCCGCGAAGAAAGGTGCTAAGTTTTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGa                                                                                                            <  2:140590/151‑1 (MQ=255)
                                      gCGATGATGGTCCCGTTCGACAGCATCAAATTCTCGGGCAACTACGGCAACATGACCGAAGTCCCCTATCAGGTTGCGAAACGTGCCGCAAAGAAAGGTGCTAAGTATTACCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCt                                                                                                              <  2:484845/149‑1 (MQ=255)
                                       cGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGAc                                                                                                           >  1:1069354/1‑151 (MQ=255)
                                        gatgatGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGAcc                                                                                                          >  2:1056933/1‑151 (MQ=255)
                                              ggTCCCGTTCGACAGCATCAATTCCTCTGGCATTTACGGCAATATGGCCGAAGTCTCCTACCGGTTTGCGAAACTGCCCGCGAAAAAAGGGGTTAACTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgc                                                                                                    <  2:160077/151‑1 (MQ=255)
                                               gTCCCGTCCGACAGCATCAAATTCTCTGGCAACTACGGAAACAGGACCGAAGTCCCCTATCAGTTTGCAAAACGGGCCGCAAAGAAAGGTGCAAAGTTTTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAg                                                                                                     <  1:1083052/149‑1 (MQ=255)
                                                 cccTTTCGACAGCACAAAATTCTCTGGCAACTCCGGAACAAGGCCCGAAGTCTCCTATCAGGTTGCAAAACGTCCCGCGAAAAAAGGTGCTAAGTTTTACCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCa                                                                                                 <  2:202961/151‑1 (MQ=255)
                                                 cccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc                                                                                                  <  2:441120/150‑1 (MQ=255)
                                                  ccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAg                                                                                                <  2:243486/151‑1 (MQ=255)
                                                  ccGTTCGACAGCATAAAATTCTGTGGCAATTGCGGCAACATGCCGGAAGTCTCCTATCAGTTGGCAAAACGTGCCGCAAAAAAAGGTGGTAAGTATTACCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAg                                                                                                <  2:914097/151‑1 (MQ=255)
                                                   cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc                                                                                                  >  1:160601/1‑148 (MQ=255)
                                                        gACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCCCCTATCAGGTTGCGAAACGTGCCGCAAAGAAAGGTGCTAATTATTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc                                                                                             <  2:983384/148‑1 (MQ=255)
                                                           accATCAATTTCTCTGGCAATTCCGGAAAAAGGACCGAATTCTCCTATCAGGTTGCAAAACGTGCGGGAAAAAAGGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGtata                                                                                       <  1:978161/149‑1 (MQ=255)
                                                             cATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataa                                                                                      >  1:414211/1‑150 (MQ=255)
                                                              ataaaaTTCTCGGGCAACTCCGGAAACATGACGGAAGTCTCTTATCAGGTTGCGAACCGTGCCGCGAAGAAAGGTCCTAAGTATTCCCACAACACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataa                                                                                      <  2:497398/146‑1 (MQ=255)
                                                                 aaaTTCTCTGGCACCGCCTGCAAAATGCCCGAATTCTCCTCCCAGTTTGGCAACGGTGCCGCGAAAAAGGGTGCTAAGTATTCCCAATCCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAAc                                                                                 <  2:1052853/151‑1 (MQ=255)
                                                                 aaaTTCTCTGGCAACTCCGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGta                                                                                         <  2:825090/143‑1 (MQ=255)
                                                                 aaaTTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGTGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataaataa                                                                                  >  1:170528/1‑150 (MQ=255)
                                                                    ttCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTAt                                                                              >  1:173141/1‑151 (MQ=255)
                                                                    ttCTCTGGCAACTACGGCAACAGGCCCGAAGTCTCTTATCAGTTTGCAAAACGGCCCGCGAAGAAAGGTGCTAAGTATTACCACTCCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTa                                                                               <  1:988165/150‑1 (MQ=255)
                                                                         tGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGt                                                                         >  1:545091/1‑151 (MQ=255)
                                                                          ggCAACTACGGAAACATGCCCGAAGTCTCCTATCAGGTTGCAAAACGTGCCGGGAAGAAGGGTGCTAAGTATTCCCCCATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGtt                                                                        <  1:762401/151‑1 (MQ=255)
                                                                          gccAACTGCGGCAACAGGACCGAAGTCCCCTTGCAGGTTGCAAAACGGACCGCGAAGAAGGGTGCTAATTATTCCCACATCGTCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCa                                                                           <  1:715850/146‑1 (MQ=255)
                                                                            cAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGtt                                                                        >  1:963952/1‑149 (MQ=255)
                                                                             aactccgaaaaCATGACCGAAGTCTCCTATCAGGTTGCGAAACGGGCCGCGAAAAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTAc                                                                      <  1:347257/141‑1 (MQ=255)
                                                                                  cGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCtat                                                                >  1:466110/1‑151 (MQ=255)
                                                                                  cGGAAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAAAAAGGTGTTAAGTATTCCCACACCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTcc                                                                   <  2:937438/148‑1 (MQ=255)
                                                                                    gCAACATGACCAAAGTCTCCTAACAGGTTGGAAAACGTGCCGAGAAGAAAGGTGCAAATTTTTCCCACACCAGCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTcc                                                                   <  2:1184888/146‑1 (MQ=255)
                                                                                      aaCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATaa                                                              >  1:547851/1‑149 (MQ=255)
                                                                                       aCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATaa                                                              >  1:144266/1‑148 (MQ=255)
                                                                                                aattctcTTATCAGTTGGCGAAACGGGCCGCAAAAAAAGGTGCTAATTATTCCCCCACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGAt                                                   <  1:976114/147‑1 (MQ=255)
                                                                                                aaGTCTCCTATCGGGTTGCAAAACGTGCGGCAAAGAAGGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCtt                                                      <  2:316987/147‑1 (MQ=255)
                                                                                                aaGTCTCCTATCAGGTTGCAAAACGTGCCGCAAAGAAAGGTGCTAAGTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATg                                                  <  1:815062/151‑1 (MQ=255)
                                                                                                    ctcCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATgccgc                                              >  2:952575/1‑151 (MQ=255)
                                                                                                      ccTATCAGGTTGCGAACCGTGCCGCGAAGAAAGGTGCTAAGTATTCCCACACCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATgccgc                                              <  2:841546/149‑1 (MQ=255)
                                                                                                      ccTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCt                                            >  1:824853/1‑151 (MQ=255)
                                                                                                      ccTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCt                                            >  2:314031/1‑151 (MQ=255)
                                                                                                      ccTATCAGGTGGCGAAACGTGCCGCGAAAAAGGGTGCAAAGTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATgccgcc                                             <  1:576782/150‑1 (MQ=255)
                                                                                                            gggTTGCGAAGCGTGCCGCGAAGAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCa                                      <  1:665858/150‑1 (MQ=255)
                                                                                                               ttGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCAttt                                   >  2:1001069/1‑151 (MQ=255)
                                                                                                               ttGCAAAACGTGCCGCGAAGAAAGGTACTAATTTTTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACACCTATAAGGCGGCTTGATGCCGCCTTTCGCAtt                                    <  1:10420/150‑1 (MQ=255)
                                                                                                                    aaaCGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGt                              >  1:379805/1‑151 (MQ=255)
                                                                                                                    aaaCGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGt                              >  2:432521/1‑151 (MQ=255)
                                                                                                                     aaCGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTc                             >  1:1278236/1‑151 (MQ=255)
                                                                                                                       cGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTcac                           >  1:10427/1‑151 (MQ=255)
                                                                                                                        gggCCGAGAAGAAAGTTGCTAATTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTcaca                          <  1:1031803/149‑1 (MQ=255)
                                                                                                                           ccGAGAAGAAGGGTGATAAGTTTTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACaa                         <  1:699512/149‑1 (MQ=255)
                                                                                                                              caaagaaAGGTGTTAATTATTCCCACACCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACaaa                        <  2:1002857/145‑1 (MQ=255)
                                                                                                                                aagaagGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAAtt                      <  1:184633/147‑1 (MQ=255)
                                                                                                                                aagaaAGGTGCTAAGTTTTCCCACACCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCTCCTTTCGCATTTTTAGTCACAAATTTc                    <  1:619057/149‑1 (MQ=255)
                                                                                                                                aagaaAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTccc                  >  1:1269657/1‑151 (MQ=255)
                                                                                                                                aaaaaaGGTGCAAAGTATTACCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAAtt                      <  2:486831/144‑1 (MQ=255)
                                                                                                                                aaaaaaGGTCCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAAttt                     <  1:395277/145‑1 (MQ=255)
                                                                                                                                   aaaGGTGCTAATTTTTACCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCcgc               <  1:1278120/151‑1 (MQ=255)
                                                                                                                                     aGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCcgcg              >  2:1191701/1‑150 (MQ=255)
                                                                                                                                         gCTAAGTATTCCCCCATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCAt         <  2:515115/151‑1 (MQ=255)
                                                                                                                                            aaGTTTTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgc      <  2:1041878/151‑1 (MQ=255)
                                                                                                                                            aaGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgc      <  2:1175914/151‑1 (MQ=255)
                                                                                                                                            aaGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtg       <  2:855005/150‑1 (MQ=255)
                                                                                                                                               tATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgcatg   >  1:372938/1‑151 (MQ=255)
                                                                                                                                                aTTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgcatgc  >  1:300129/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGC  >  NC_000913/1679157‑1679451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: