Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9180AC42.7% 140.2 / 15.8 75intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
Reads supporting (aligned to +/- strand):  ref base A (38/5);  new base C (0/32);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 2.79e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC  >  NC_000913/2001775‑2002066
                                                                                                                                                  |                                                                                                                                                    
ataGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCccgc                                                                                                                                                <  1:181751/150‑1 (MQ=255)
   gagTGAATTCACGATAAACAGCCCTGCTTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACGGGTAAATTCCAGGCAAAAAAAAACCccgccg                                                                                                                                              <  1:772563/149‑1 (MQ=255)
    agTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATTGGACGATTAGTGGGTGAAATGAGGGGGTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCgg                                                                                                                                             >  2:746455/1‑149 (MQ=255)
     gTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTgg                                                                                                                                          >  2:61364/1‑151 (MQ=255)
       gAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCg                                                                                                                                        >  2:1292871/1‑151 (MQ=255)
        aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCgg                                                                                                                                       >  2:881200/1‑151 (MQ=255)
           tCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCgggg                                                                                                                                     >  2:219314/1‑150 (MQ=255)
             atgATACACAGCCCGGCATTTTAGGAGTTATCGGCTGTATTTTCCGTTTCTGGGGGGTTTTTAAACGGACGTTTAGTGGGTGAAGGGAGGGTTTATTGGGGGGTTACGGTTAAATTCCAGTCAGAAAAAACCCCCGCCGGTGGCGGGGAAg                                                                                                                                  <  2:815422/149‑1 (MQ=255)
              cGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGc                                                                                                                                 >  2:950649/1‑151 (MQ=255)
                  aaaCAGCCTTGGTTTATTGGGTTTATGGGAATGATTTTCCTTTTCTGCGGGGTTTTTAATCGGACGATTAGGGGGTAAAAGGAGGGTTTTTTGGGGGTTAACAGGAAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGaa                                                                                                                                   <  2:159956/145‑1 (MQ=255)
                         ccTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGAc                                                                                                                      >  2:1113958/1‑151 (MQ=255)
                           tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACa                                                                                                                     >  1:1213306/1‑150 (MQ=255)
                           tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACa                                                                                                                     >  2:586518/1‑150 (MQ=255)
                               ttATATGAGTTATCGGCATGATTATCCGTTTCTGGAGGGTTTTTAATCGGACAATTAGTGGGTAAAATGGGGGTTTTTTTGGGGTTTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                                 <  2:924687/150‑1 (MQ=255)
                               ttATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                                 >  2:293602/1‑150 (MQ=255)
                               taatatGGTTTATCGGTGTTTTTACCCTTTTCGGGGGGTTTTTTAATCGAACGATTAGTGGGTAAAATGGGGGTTTATTTGGGGTTTACAGGTAAATCCCAGCAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGAc                                                                                                                      <  1:428188/143‑1 (MQ=255)
                                  tatGATTTATGGGCATGATTATCCTTTTCTGCGGGTTTTTTAAGGGGACGATTGGTGGGGAAAATGAGGGTTTATTTGGGGGTTACGGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                              <  2:777073/150‑1 (MQ=255)
                                     gatttATCGGCATTATTTCCCTTTTCTGCGGGTTTTTTAATCGGACGATTAGGGGGGAAAAGGGGGGTTTTTTTGGGGTTTACGGGTAATTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                          <  1:1221845/148‑1 (MQ=255)
                                      aGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                          >  1:227189/1‑150 (MQ=255)
                                       gTTATCGGCATGATTATCCGTTTCTGCAGGTTTTTTAATCGGACGATTAGGGGGTAAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                          <  2:294471/149‑1 (MQ=255)
                                        ttATCGGCATGATTATCCGTTTCTGCGGGTTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGTTTATTGGGGGGTTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTa                                                                                                         <  2:1140381/149‑1 (MQ=255)
                                        taaTCGGCATGATGACCCTTTTCTGGAGGGTTTTTAATGGGACGATTAGGGGGGGAAAGGAGGGTTTTTTTGGGGGTAAGGGGTAATTCCCAGGAAAAAAAAACCTCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                        <  1:902686/148‑1 (MQ=255)
                                          aTCGGCTTAATTATCCGTTTCTGCAGGGTTTTAAATCGGACGATTGGGGGGTGAAATGGGGGTTTATTGGGGGTTTACAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGt                                                                                                      <  2:474389/150‑1 (MQ=255)
                                           tCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTg                                                                                                    >  1:1004802/1‑151 (MQ=255)
                                            cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGc                                                                                                   >  1:1186591/1‑151 (MQ=255)
                                            cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGc                                                                                                   >  2:211804/1‑151 (MQ=255)
                                                aTGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCgg                                                                                                >  2:981786/1‑150 (MQ=255)
                                                  gATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                             >  2:73653/1‑151 (MQ=255)
                                                    ttATCCTTTTCTGCGGGTTTTTTAACCGGACGATTAGGGGGTAAAAGGGGGGTTTATTGGGGGTTTAAAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                              <  2:59309/148‑1 (MQ=255)
                                                     tATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcgg                                                                                          >  1:654584/1‑151 (MQ=255)
                                                       tCCGTTTCTGCAGGGCTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggcg                                                                                        >  2:174899/1‑151 (MQ=255)
                                                        ccGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                                       >  1:52453/1‑151 (MQ=255)
                                                           tttcggggggttttttAATCGGACATTTGGTGGGTGAAATGGGGGTTTTTTTGGGGTTTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                                       <  2:355225/137‑1 (MQ=255)
                                                           tttCTGCGGGTTTTTTAATCGGACGATTGGTGGGTAAAAGGGGGTTTTTTTTGGGGGTTACAGATAAATCCCAGGAAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggc                                                                                         <  1:975281/146‑1 (MQ=255)
                                                           tttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                                     >  1:1259536/1‑150 (MQ=255)
                                                             ttgggggggTTTTTAAAGCGGATGATTATGGGGTAAAAGGGGGGGTTTTTTGGGGTTTACAGGAAAATTCCAGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaaa                                                                                    <  2:1036676/144‑1 (MQ=255)
                                                                gcgggttttttAATGGGACGATTAGGGGGAAAAATGAGGGTTTTTTTGGGGGTTACAGGTAATTCCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGc                                                                                 <  2:337179/143‑1 (MQ=255)
                                                                   gggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                            >  1:687312/1‑151 (MQ=255)
                                                                     gTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                          >  2:709720/1‑151 (MQ=255)
                                                                      ttttaAATGGGACGATTAGGGGGTAAAAGGGGGGTTTTTTTGGGGGTTACAGGAAATTTCCGGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                          <  2:1259616/150‑1 (MQ=255)
                                                                         ttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                      >  2:682775/1‑151 (MQ=255)
                                                                          tAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     >  2:1124530/1‑151 (MQ=255)
                                                                          tAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     >  2:620067/1‑151 (MQ=255)
                                                                            aaTCGGACGATTAGTGGGTGAAATGGGGGTTTATTGGGGGTTTCCAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     <  2:742592/150‑1 (MQ=38)
                                                                            aaTCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     >  1:1214937/1‑150 (MQ=255)
                                                                            aaTCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     >  2:71919/1‑150 (MQ=255)
                                                                            aaTCGGACAATTAGGGGTGAAAATGGGGGTTTTTTTGGGGGTAAAGGTTAAATTCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                      <  1:1181976/149‑1 (MQ=25)
                                                                             aggggaggaTTGGGGGGTAAAAGGAGGGGTTTTTTGGGGGTTAAAGGTAAATCCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                   <  1:322153/148‑1 (MQ=37)
                                                                               cGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                                  >  1:804787/1‑150 (MQ=255)
                                                                                ggACGTTTGGGGTGTAAAAGGAGGGTTTTTTTGGGGTTTACAGAAAATTTCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                                  <  1:755140/149‑1 (MQ=25)
                                                                                ggACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa                                                                 >  2:78876/1‑150 (MQ=255)
                                                                                   cGATTAGTGGGTGAAATGAGGGTTTATTGGGGGGTTACAGTTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                                             <  2:874927/151‑1 (MQ=255)
                                                                                      ttAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgtg                                                          >  2:1019037/1‑151 (MQ=255)
                                                                                       tAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                                         >  1:1036277/1‑151 (MQ=255)
                                                                                          tGGGTGAAATGAGGGGATATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGCTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaa                                                       >  2:399604/1‑150 (MQ=255)
                                                                                                 aaaggggggtTTATTGGGGGTTTACTGATAATTCCCGGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCa                                                  <  2:726965/143‑1 (MQ=25)
                                                                                                     gAGGGGTTATTTGGGGGTTACAGATAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaaa                                             <  2:983334/150‑1 (MQ=255)
                                                                                                       gggtTTTTTTGGGGGTTACAGATAATTTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAt                                            <  1:1298010/149‑1 (MQ=37)
                                                                                                       gggtTTTTTTGGGGGTTAAAGAAAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTg                                          <  1:184397/151‑1 (MQ=25)
                                                                                                       gggtTTATTTGGGGTTAAAAGTTAATTCCCAGGAAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAtt                                           <  1:847592/150‑1 (MQ=25)
                                                                                                            tATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                     >  2:31402/1‑151 (MQ=255)
                                                                                                             ttttGGGGGTTAAAGGTAAATCCCAGAAAAAAAAACCCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                     <  1:1292790/149‑1 (MQ=25)
                                                                                                              tttGGGGGTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                     <  1:100833/149‑1 (MQ=255)
                                                                                                                tGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAAc                                 >  2:309098/1‑151 (MQ=255)
                                                                                                                 gggggTTAAAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                                   <  2:117763/148‑1 (MQ=255)
                                                                                                                  gggtTTACAGGTAAATTCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACtt                               <  2:1213383/151‑1 (MQ=255)
                                                                                                                   gggTTACAGGAAAATCCCAGGCAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                              <  2:651141/151‑1 (MQ=255)
                                                                                                                    ggTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                             >  2:35544/1‑151 (MQ=255)
                                                                                                                      ttACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                             >  2:281569/1‑149 (MQ=255)
                                                                                                                       tACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg                          >  1:624265/1‑151 (MQ=255)
                                                                                                                            ggTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg                           <  1:470267/146‑1 (MQ=255)
                                                                                                                             gTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg                      >  2:980944/1‑150 (MQ=255)
                                                                                                                               aaaTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCt                       <  1:1144934/147‑1 (MQ=255)
                                                                                                                               aaaTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg                      <  1:330007/148‑1 (MQ=255)
                                                                                                                               aaaTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg                      <  1:1192222/148‑1 (MQ=255)
                                                                                                                                             aaaaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCa     <  1:1287847/151‑1 (MQ=255)
                                                                                                                                              aaaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAg    <  2:1041493/151‑1 (MQ=255)
                                                                                                                                                 aaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGTCCTGATAAGCGCAGCGCATCAGGc  >  2:1226646/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC  >  NC_000913/2001775‑2002066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: