Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,277	0	A	C	46.6%	102.6 / 28.4	74	V274G (GTA→GGA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (34/5);  new base C (0/34);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 1.34e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACT  >  NC_000913/3443130‑3443426                                                                                                                                                        |                                                                                                                                                      gACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑Accc                                                                                                                                                    <  1:503591/151‑1 (MQ=255)  aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑Acccc                                                                                                                                                   <  1:766611/151‑1 (MQ=255)    gCATAGAGTAACACATAAAGCGGTTGCCCAGGCTTCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCg                                                                                                                                                 <  1:176981/151‑1 (MQ=255)     cATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGc                                                                                                                                                >  2:1023260/1‑151 (MQ=255)     cATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGc                                                                                                                                                <  2:898702/151‑1 (MQ=255)      aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGcc                                                                                                                                               <  1:368298/151‑1 (MQ=255)        agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCat                                                                                                                                             >  2:348151/1‑151 (MQ=255)         gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCat                                                                                                                                             >  2:1279952/1‑150 (MQ=255)          agTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCatat                                                                                                                                           >  1:517254/1‑151 (MQ=255)               cacaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACt                                                                                                                                      >  1:1138793/1‑151 (MQ=255)                 caTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACttt                                                                                                                                    >  1:1170620/1‑151 (MQ=255)                    aagggggtgccccGGGCTGAAAATACAGCAAATTTGTTGCCAGCCGGTTCCACCCAG‑TCCCCCCCCCGAACCATGACGCGATGGTCGCGGGAAAAAAAATAATACGGGAAGCG‑AAGTTTGCGGGGA‑TT‑CCCCCCGCCATATTCACTTTCa                                                                                                                                  <  2:673868/140‑1 (MQ=255)                    aaaGCGGTTGCCCGGGCTGAAAATACAGCAAATTTGTTGTCAGCCGTTTCCAACCAG‑TACCCCCCCCGAACCATGACGGGATGGTCGCGGGAAACAAAATAATAGTGGAAGCG‑AAGATGGCGGGTT‑TT‑CCCCCCGCCATATTCACTTTCAg                                                                                                                                 <  2:531299/151‑1 (MQ=255)                         ggtgcccgaGGCTGAAAATACAGAGAAATTGTTGTCAGCCAGTTCCAACCAG‑TCCCCCCCCCGAACCATGACGCGATGGTCGCCGGAAACAAAATAATACTGGAAGCA‑AAGATGGCGGGGA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                            <  2:882582/143‑1 (MQ=255)                         ggTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGt                                                                                                                             >  1:660937/1‑150 (MQ=255)                           ttccccAGGTTGAAAATGCAGCAAATTTTTTGCCAGCCAGTCCCACCCAG‑TCCCGCCCCCGAACCATGCCGCGATGGCCGCGGGAAACAGAAAAATACTGGAAGCA‑AAGATTGCCGGGA‑TT‑CCCCCCGCCATATTCACTTTCAGCgg                                                                                                                              <  2:1196869/144‑1 (MQ=255)                            tGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                         >  1:1156285/1‑151 (MQ=255)                              cccGGGCTAAAAATACAGCAAATTTGTTGTCCCCCAGTCCCACCCGG‑CCCCCCCCCCGACCCAGGCCGCGGTGGCCGCGGGAAACAAAAAAATAGTGGAAGAA‑AAAATTG‑CGGGA‑TTCCCCCCCGCCATATTCACTTTCAGCGGTaaa                                                                                                                          <  1:672448/148‑1 (MQ=255)                              cccAGGCTCCAAAAACAGCGAAATTGTTGTCACCCAGTCCCACCCAG‑CCCCCCCCCCAAACCAGGGCGCGATGGTCGCGGGGAACAGAATAATACTGGAAGCA‑AAGATTGCCGGGA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAAtgt                                                                                                                       <  2:259453/151‑1 (MQ=255)                                cAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                                     >  1:556468/1‑151 (MQ=255)                                cAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                                     >  2:2326/1‑151 (MQ=255)                                  ggCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                                   >  2:1265881/1‑151 (MQ=255)                                  ggCTGAAAATACAGCAAATTTGTTGTCGGCCTTTCCCAACCAG‑TCCCCCCCCCAAACCATGACGCGAGGGTCGCGGGAAACAAAAAAATACGGGAAGCG‑AAGATTGCCGGGA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                                   <  2:1243978/151‑1 (MQ=255)                                         aaTACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                            >  1:563904/1‑151 (MQ=255)                                          aTACAGCAATTTTGTTGTCA‑CCAATTCCAACCCGTTCCCCCCCCCGACCCATGCCGCGCGGGCCCCGGGAAAAAAAAAAATAC‑GGAAGGGAAATTTTGCGGGTT‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgca                                                                                                           <  1:255594/151‑1 (MQ=255)                                           tACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                            >  2:1245906/1‑149 (MQ=255)                                                  aattttttgcCACCCAGTCCCACCCAG‑ACCCCCCCCCGACCCATGACGCGATGGCCGCCGGGAAAAAAATAATACTGGAAGCA‑AAGTTGGCCGGGA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                       <  1:393311/141‑1 (MQ=255)                                                  aatttgttgCCACCCATTCCAAACCAG‑CCCCCCCCCCGACCAAGGCCGGTATGTTCGCGGGGAAAAAAATAATACGGGAAGAG‑AAG‑TTGGCGGGATTT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagca                                                                                                        <  1:1065338/143‑1 (MQ=255)                                                  aaaTTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                   >  1:624511/1‑151 (MQ=255)                                                        ttgtCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                              >  1:575284/1‑150 (MQ=255)                                                        ttgtCAGCCAGTCCCACCCAG‑TCCCCCCCCCAACCCAGGCCGCGATGGCCGCGGGAAAAAAAAAAATACTGGAAGCG‑AAGTTGGCGGGGA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacga                                                                                               <  1:390318/149‑1 (MQ=255)                                                            cAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                                                                         >  1:293195/1‑151 (MQ=255)                                                               ccgttcccAACCAG‑TCCCCCCCCCGACCCAGGACGGGAGGGTCGCGGGAAAAAAAATAATTCGGAAGGCA‑AAGTTGGCGGGGA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGAcc                                                                                          <  2:1044967/141‑1 (MQ=255)                                                                cAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCt                                                                                     >  2:621009/1‑151 (MQ=255)                                                                       aaCCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                              >  2:795342/1‑151 (MQ=255)                                                                        acccagccccccccccGACCCATGACGCGAGGGTCGCGGGAAAAAAAATAATACTGGAAGCA‑AAGATTGCGGGGA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                                  <  2:989617/136‑1 (MQ=255)                                                                         ccAG‑TCCCCCCCCCAAACCATGGCGCGTTGGTCGCGGGAAAAAAAATAATACTGGAAGGG‑AAGATTGCCGGTT‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcg                                                                            <  1:310129/151‑1 (MQ=255)                                                                          ccagccccGCCCCCGACCCAGGACGCGATGGTCGCCGGGAAAAAAAAAATACTGGAAGCG‑AAGATTGCGGGGA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcg                                                                            <  2:334732/145‑1 (MQ=255)                                                                             gtcccccccccGAACCATGCCGCGGGGGTCGCCGGAAAAAAAATAATCCGGGAAGCG‑AAGATTGCGGGAA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgc                                                                           <  2:1123434/143‑1 (MQ=255)                                                                              tACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                         >  2:450517/1‑150 (MQ=255)                                                                                  ccccccGACCCAGGACGCGTGGTTCGCGGGAAAAAAAATAATCCGGGAAGCG‑AAGTTTGCGGGGA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                    <  1:365719/150‑1 (MQ=255)                                                                                   cccccGAACCATGCCGGGATGGCCGCGGGAAACAGAATAATACGGGAAGCA‑AAGTTTGCCGGAA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                    <  2:1150589/150‑1 (MQ=255)                                                                                    ccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                                  >  1:975322/1‑151 (MQ=255)                                                                                       caagcataggcgcgAGGGTCGCGGGAGAGAGAAGAATATTGGAAGGA‑AGGATGGCGGGGA‑TT‑GCGGCCGCCATATTGACGTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACCCGGGGACGTTGCTGACGTTTcgc                                                                           <  1:387142/129‑1 (MQ=255)                                                                                           ccaggccgggATGGTCGCCGGAAAAAAAATAATACTGGAAGCA‑AAGTTTGCCGGTA‑TC‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATg                                                           <  1:1022865/142‑1 (MQ=255)                                                                                             aTGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg                                                         >  2:1156188/1‑151 (MQ=255)                                                                                                  gggggggTCGCGGGGAAAAAAAAAATCCGGAAAGCA‑AAGTTTGCGGGTA‑TC‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg                                                         <  1:62413/141‑1 (MQ=255)                                                                                                  gcgATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGt                                                     >  2:338918/1‑150 (MQ=255)                                                                                                     aTGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAc                                                 >  2:573948/1‑151 (MQ=255)                                                                                                             cGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                          >  1:748755/1‑150 (MQ=255)                                                                                                              ggaaaCAAATTAATACTGGAAGCG‑AAGTTTGCCGGGA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                          <  2:520125/146‑1 (MQ=255)                                                                                                                 aaaaaaataatACGGGAAGCG‑AAGTTTGCCGGGA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                      <  1:1254571/145‑1 (MQ=255)                                                                                                                 aaCAAAATAATATGGAAAGAG‑AGGTTTGGGGGAA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaacaa                                     <  1:97672/151‑1 (MQ=255)                                                                                                                 aaCAAAATAATACGGAAAGGG‑AAGATTGCGGGAA‑TC‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                      <  1:423122/150‑1 (MQ=255)                                                                                                                   cAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                   >  1:222100/1‑151 (MQ=255)                                                                                                                    aGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                   >  1:253970/1‑150 (MQ=255)                                                                                                                     aaataatACGGGAAGGA‑AAGTTGCCCGGAA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                 <  1:94065/150‑1 (MQ=255)                                                                                                                       ataataCGGGAAGCG‑AGGATGGCGGGGA‑TC‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaa                               <  2:726060/151‑1 (MQ=255)                                                                                                                         aataCGGGAAGCG‑AAGATGGCCGGGT‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                              <  1:1234008/150‑1 (MQ=255)                                                                                                                          ataCTGGAAGCG‑AAGATTGCCGGTT‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                              <  1:227574/149‑1 (MQ=255)                                                                                                                               ggAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                       >  2:628922/1‑151 (MQ=255)                                                                                                                                ggaggagaagtgtGCGGGAA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGa                         <  1:890467/137‑1 (MQ=255)                                                                                                                                     g‑AAGTTGGCCGGTA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCa                   <  1:195739/149‑1 (MQ=255)                                                                                                                                     g‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt                 >  2:523851/1‑151 (MQ=255)                                                                                                                                        agtatGCTGGGA‑TA‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGGCACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg                <  1:105486/146‑1 (MQ=255)                                                                                                                                         gagtcccGGGA‑TT‑AAACCAGCCATATTCACTTTCGGCGGTAAATGTGTGCTGTGTGCAGCATAGACACGACGACCTTGCTGACGTTGCGCGCAGTTTACCACACTGCGGCGTCGACCACGCTCAACAAATACAACaaa                           >  1:1048619/6‑138 (MQ=255)                                                                                                                                           ttGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa             >  2:116688/1‑150 (MQ=255)                                                                                                                                            tGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaaa            >  1:219859/1‑150 (MQ=255)                                                                                                                                            tGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat           >  1:500041/1‑151 (MQ=255)                                                                                                                                                ggta‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact        <  1:609086/147‑1 (MQ=255)                                                                                                                                                gggA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatacta       <  2:147372/151‑1 (MQ=255)                                                                                                                                                gggA‑TT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac         <  2:1146351/149‑1 (MQ=255)                                                                                                                                                      t‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaatact  >  2:414748/1‑151 (MQ=255)                                                                                                                                                      t‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaatact  >  2:734731/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                      GACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAG‑TACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCG‑AAGATTGCCGGGA‑TT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACT  >  NC_000913/3443130‑3443426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑