Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913354,2880TG49.3% 90.7 / 39.3 75V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (5/33);  new base G (37/0);  total (42/33)
Fisher's exact test for biased strand distribution p-value = 2.79e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354138‑354434
                                                                                                                                                        |                                                                                                                                                    
aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGt                                                                                                                                                      <  2:764586/151‑1 (MQ=255)
   ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGa                                                                                                                                                    <  1:284369/150‑1 (MQ=255)
            gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCt                                                                                                                                          <  1:181374/151‑1 (MQ=255)
             tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTGGGGGGGGAAGATGCGCt                                                                                                                                          >  2:697742/1‑150 (MQ=255)
              gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGGGGAAGATGCGCT‑g                                                                                                                                        >  2:326990/1‑150 (MQ=255)
                cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑Gaaa                                                                                                                                     <  2:164480/151‑1 (MQ=255)
                    gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGG‑GGTTGGGGGGAAA‑ATGGGCG‑GGAAgggg                                                                                                                                 >  1:677358/1‑150 (MQ=255)
                    gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGATGCGCT‑GAAAggg                                                                                                                                  >  2:784177/1‑150 (MQ=255)
                     aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCGTCCGGGCGTTGCCGAAGTGGC‑GGG‑GGGTGGGGGGGAAGATGGGCT‑GGAAggg                                                                                                                                  >  1:1140417/1‑149 (MQ=255)
                       ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAgg                                                                                                                              <  1:813357/151‑1 (MQ=255)
                          tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtg                                                                                                                            <  2:44102/150‑1 (MQ=255)
                            tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGGGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTTGGGGGGAAGATGCGCC‑GGAAGGGGAggggggg                                                                                                                         >  2:1086219/1‑149 (MQ=255)
                            tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGAAGCGCT‑GAAAGGGCAgggggcg                                                                                                                         >  2:101649/1‑151 (MQ=255)
                             cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtggcg                                                                                                                         <  2:997399/150‑1 (MQ=255)
                                 ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtggcggtg                                                                                                                      <  1:599417/149‑1 (MQ=255)
                                  tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTTGGGGGGAAGATGCGCC‑GGAAGGGGAggggggggggg                                                                                                                     >  1:32532/1‑149 (MQ=255)
                                  tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCT‑GGAAGGGCAGggggcgggggcg                                                                                                                   >  1:1084311/1‑151 (MQ=255)
                                       tcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAAATGCGCT‑GAAAGGGGAGGGGGGGGGGGCGttt                                                                                                                >  2:414663/1‑149 (MQ=255)
                                          ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCAt                                                                                                           <  1:1012480/151‑1 (MQ=255)
                                           gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCAtt                                                                                                          <  1:339400/151‑1 (MQ=255)
                                           gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGG‑GGGTGGGGGGGAAGATGGGCT‑GGAAGGGGAggggggggggggggttttcctt                                                                                                          >  1:164131/1‑140 (MQ=255)
                                               aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGa                                                                                                      <  2:803244/151‑1 (MQ=255)
                                                cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGG‑GGGTGGGGGGGAAGATGGGCT‑GAAAAGGGAGGTGGGGGGGGCGTTTGTTAtttc                                                                                                        >  2:1059693/1‑146 (MQ=255)
                                                  gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa                                                                                                     <  2:350674/149‑1 (MQ=255)
                                                      aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAaga‑gag                                                                                              <  2:1269432/151‑1 (MQ=255)
                                                      aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGAAGCGCT‑GAAAGGGGAGGGGGGGGGGGGGTTTTTCATTCCGaaa                                                                                                    >  1:244283/1‑146 (MQ=255)
                                                      aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGC‑GGGTGGGGGGAAAGGATCGCT‑GAAAAGGGAGGGGGGGGGGGGGGTTGTCATTTCGCAaga‑ggg                                                                                              >  2:1132577/1‑149 (MQ=255)
                                                        gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGATGCGCT‑GGAAGGGGAGGGGGCGGGGGGGGTTGTCATTTCGAAaga‑gag                                                                                              >  1:590333/1‑149 (MQ=255)
                                                           aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGAGGGGCT‑GGAAGGGGAGGGGGCGGGGGCGGTTGTTCTTTCGCAAGA‑GAGCggc                                                                                          >  1:621796/1‑148 (MQ=255)
                                                             tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGGAAGATGGGCT‑GAAAGGGCAGGGGGCGGTGGGGGTTGTCATTCCGAAAGA‑GGGCGACAGt                                                                                       >  2:1136557/1‑151 (MQ=255)
                                                              aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGt                                                                                       <  1:1019017/150‑1 (MQ=255)
                                                               aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCt                                                                                     <  2:45740/151‑1 (MQ=255)
                                                                   tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGa                                                                                  <  1:814748/150‑1 (MQ=255)
                                                                   tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTTGGGGGGAAGAAGCGCTGGAAAGGGC‑GGGGGGGGGGGCGGTTGTCCTTCCGAAAGA‑GAGGGGCAGTc                                                                                      >  2:560026/1‑146 (MQ=255)
                                                                   tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGG‑GGTTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGGGTTTGTCATTTCGAAAGA‑GAGCGACAGTCTgg                                                                                   >  2:1060254/1‑149 (MQ=255)
                                                                     ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGa                                                                               <  2:838656/151‑1 (MQ=255)
                                                                     ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGa                                                                               <  2:335376/151‑1 (MQ=255)
                                                                     ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGa                                                                               <  2:225267/151‑1 (MQ=255)
                                                                         gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGt                                                                           <  1:991659/151‑1 (MQ=255)
                                                                           aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGa                                                                         <  1:987822/151‑1 (MQ=255)
                                                                           aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGa                                                                         <  1:678987/151‑1 (MQ=255)
                                                                              ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGAtgt                                                                      <  2:1115170/151‑1 (MQ=255)
                                                                              ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGC‑GGGTGGGGGGGAAGAGGGGCT‑GTGAGGGCCGGCGGCGGGGGGGCTTGTTATTCCGCAAGA‑GGGCGACAGGCTGGAAGAtaggggt                                                                        >  1:1221807/1‑143 (MQ=255)
                                                                                      aCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCT‑GGAAGGGCAGGTGGCGGTGGCGGTTGGCATTTCGAAAGA‑GAGGGACAGGCTGGAAGATTGTGAtgttggg                                                                  >  1:1043975/1‑143 (MQ=255)
                                                                                       cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACt                                                              <  1:1148392/150‑1 (MQ=255)
                                                                                          gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGGGGAAGAAGCGCT‑GAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGGAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGc                                                           >  1:292967/1‑150 (MQ=255)
                                                                                          gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAAATGGGGT‑GAAAGGGCAGGGGGGGGGGGGGGTTGTTCTTTCGGAAGG‑GAGGGACAGTCTGGGAGATAGTGCTGTggg                                                                   >  2:1181446/1‑141 (MQ=255)
                                                                                             agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTTGGGGGGAAA‑ATGCGCT‑GGAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGGAGATCGGGATGGGGGGCACCCGCa                                                          >  1:1100095/1‑148 (MQ=255)
                                                                                              gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                      <  2:877316/151‑1 (MQ=255)
                                                                                              gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                      <  2:487280/151‑1 (MQ=255)
                                                                                               aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCT‑GGAAAGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCaa                                                         >  2:404051/1‑147 (MQ=255)
                                                                                               aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGAAGGGCT‑GGAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACCCGCAag                                                        >  2:608505/1‑148 (MQ=255)
                                                                                                  gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                   <  2:1277495/150‑1 (MQ=255)
                                                                                                   aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGCGGGGGGAAAGATGCGAT‑GAAAGGGCAGGGGGCGGGGGCGGTTGTCATTTCGTAAAA‑GAGCGAAAGTTTGGAAGATAGTGGTGTGGGGCACTTGCAagag                                                      >  2:588036/1‑146 (MQ=255)
                                                                                                    agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGC‑GGTTTGGGGGGAAAATGGGCTGGAAAGGGC‑GGGGGGGGGGGGGTTTGGCAATGCGAAAGAGGAGCG‑CAGTCTGGGAGGTCGTGAGGTGGCGCACCCCCAACAGGAAGCg                                                >  1:208031/1‑151 (MQ=255)
                                                                                                     gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCT‑GAAAGGGGCGGGGGGGGGGGCGGTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGGAGATCGGGATGTGGGGCACTCGCAAGAGAAgg                                                  >  2:979638/1‑148 (MQ=255)
                                                                                                      agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAt                                              <  1:1132505/151‑1 (MQ=255)
                                                                                                          tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGTGGGGGGGGGGGTTGTCATTTCGAAAGA‑GAGCGACAGTCTGGGAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGGTTAt                                           >  2:218008/1‑150 (MQ=255)
                                                                                                            tctcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAAAAGCGCT‑GAAAGGGGCGGGGGGGGGGGGGGTTGTCATTCCGGAAGA‑GAGCGACAGTCTGGGAGATCGGGGTGTGGCGCCCCCGCAAAAGAAGGCGGTTATggg                                        >  1:1146179/1‑150 (MQ=255)
                                                                                                            tctcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAAATTCGCT‑GGAAGGGGAGGGGGGGGGGGGGTTTGTCCTTCCGAAAAA‑GAGCGACAGGCTGGAAGATCGGGATGTGGCGGGCTAGCCAGAGGAGGGGATTATgg                                         >  2:531315/1‑150 (MQ=255)
                                                                                                              tcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGAAGCGCT‑GAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATTgcg                                       >  2:471539/1‑150 (MQ=255)
                                                                                                               ccAGCCGTCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcgc                                      <  2:1140489/150‑1 (MQ=255)
                                                                                                                    cATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTCGCAAGAGAAGGCGATTATGGCGCtggggg                                >  2:917438/1‑151 (MQ=255)
                                                                                                                     aTCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGAAAGA‑GAGCGACAGGCTGGAAGATCGGGATGTGGGGCACTCGCAAGAGAAGGCGATTATTGCGCtggtgg                                >  2:997217/1‑150 (MQ=255)
                                                                                                                      tCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGAAGTGCT‑GGAAGGGCAGGGGGGGGGGGGGGTTGTCATTTCCGAAGA‑GAGCGACAGTCTGGAAGATTGGGTGGTGGGGCACTCGCACGAGAAGGGGGGTATGGCGGtggggg                                >  1:867476/1‑149 (MQ=255)
                                                                                                                       ccGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGATGGGCT‑GAAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGGGGTTATGGCGCtggtgg                                >  1:1056290/1‑148 (MQ=255)
                                                                                                                            cGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGTGGCGGTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGGGGACAGcc                          >  2:808743/1‑149 (MQ=255)
                                                                                                                                    aaGTGGC‑GGT‑GGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCa                 >  1:1231482/1‑150 (MQ=255)
                                                                                                                                      gTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACt              <  2:878072/151‑1 (MQ=255)
                                                                                                                                        ggC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt            <  1:770886/151‑1 (MQ=255)
                                                                                                                                         gC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg           >  1:318435/1‑151 (MQ=255)
                                                                                                                                          c‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg          >  1:1164712/1‑151 (MQ=255)
                                                                                                                                          c‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg          >  1:987300/1‑151 (MQ=255)
                                                                                                                                                    ggggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg  <  1:784118/151‑1 (MQ=255)
                                                                                                                                                    ggggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg   <  1:500751/150‑1 (MQ=255)
                                                                                                                                                    ggggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg   <  2:137702/150‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                    
ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGA‑GAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354138‑354434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑