Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG44.0% 166.6 / 44.4 101V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (2/54);  new base G (44/0);  total (47/54)
Fisher's exact test for biased strand distribution p-value = 2.10e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

CTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAA  >  NC_000913/599431‑599723
                                                                                                                                                 |                                                                                                                                                    
cTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGGAGTGGCGGGCGgggggggg                                                                                                                                                  >  4:1210323/1‑148 (MQ=255)
    acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCt                                                                                                                                             <  4:464131/151‑1 (MQ=255)
    acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTcc                                                                                                                                              >  4:235531/1‑150 (MQ=255)
      gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGgggggggggttc                                                                                                                                              >  2:114403/1‑142 (MQ=255)
      gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGc                                                                                                                                           <  1:356807/151‑1 (MQ=255)
        cTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCg                                                                                                                                          >  1:1225947/1‑150 (MQ=255)
        cTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTcc                                                                                                                                              >  1:1280760/1‑146 (MQ=255)
          ttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCAGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGCAGTGGCGGGGGGGGCGGCGACCCTCCGac                                                                                                                                        >  4:237612/1‑149 (MQ=255)
           tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTccctcgctcc                                                                                                                                      >  4:1419579/1‑143 (MQ=255)
            aaTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATcc                                                                                                                                      <  4:748305/150‑1 (MQ=255)
             aTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCgg                                                                                                                                    <  1:555827/151‑1 (MQ=255)
             aTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCgg                                                                                                                                    <  1:1146039/151‑1 (MQ=255)
             aTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCgg                                                                                                                                    >  1:359750/1‑151 (MQ=255)
               cacaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCa                                                                                                                                  <  4:608749/151‑1 (MQ=255)
               cacaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCa                                                                                                                                  <  4:1370716/151‑1 (MQ=255)
                acaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATCCTGCGATTCgg                                                                                                                                    >  1:329755/1‑148 (MQ=255)
                 caTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCaa                                                                                                                                 <  4:730564/150‑1 (MQ=255)
                  aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGCcaa                                                                                                                                >  2:175311/1‑150 (MQ=255)
                    cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGGTCTGCGGGCCCGCaaaaa                                                                                                                              >  4:1320936/1‑150 (MQ=255)
                    cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGGCCGGCGCTCCGGCaaaa                                                                                                                               >  4:475190/1‑149 (MQ=255)
                    cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCaaaaa                                                                                                                              <  2:160502/150‑1 (MQ=255)
                     gTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACg                                                                                                                            <  4:920252/151‑1 (MQ=255)
                     gTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCcaaaa                                                                                                                              >  1:726083/1‑149 (MQ=255)
                          aaaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCga                                                                                                                       <  1:254675/151‑1 (MQ=255)
                          aaaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCAAAAACGccc                                                                                                                         >  1:736451/1‑149 (MQ=255)
                           aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGATCCCGCCAAAAAGccc                                                                                                                         >  4:940789/1‑148 (MQ=255)
                             aGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGGCCGGCAAAAAAGccc                                                                                                                         >  4:708374/1‑146 (MQ=255)
                             aGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCCGCGAAAAACCCCgcg                                                                                                                      >  4:861747/1‑147 (MQ=255)
                                  gTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGCAAAAAAGCCCGGGAAggg                                                                                                                 >  1:884650/1‑147 (MQ=255)
                                    gAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATc                                                                                                              <  1:490651/150‑1 (MQ=255)
                                    gAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCCGCGATCCGGCAAAAAAGCCCGAGAAGGGATcg                                                                                                             >  1:17334/1‑151 (MQ=255)
                                       aaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgccg                                                                                                          <  1:1115176/151‑1 (MQ=255)
                                        aTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTTCCGCGATCCCGCCAAAAAGCCCGAGGAGGGaatgcc                                                                                                           >  3:14403/1‑144 (MQ=255)
                                          ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCgccgcc                                                                                                        <  4:294754/150‑1 (MQ=255)
                                          ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCgccgcc                                                                                                        <  4:637738/150‑1 (MQ=255)
                                            cGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCG‑Tg                                                                                                    >  4:1193672/1‑151 (MQ=255)
                                               gCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGaa                                                                                                  <  1:1100363/150‑1 (MQ=255)
                                                 ccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGa                                                                                               <  3:114398/151‑1 (MQ=255)
                                                 ccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGGAGGGATCGCCGCCG‑GGAAgg                                                                                                >  1:472694/1‑150 (MQ=255)
                                                  cGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGAc                                                                                              <  1:1212688/151‑1 (MQ=255)
                                                  cGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑GGAAgg                                                                                                >  1:718245/1‑149 (MQ=255)
                                                  cGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAAATAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGAc                                                                                              <  1:1224978/151‑1 (MQ=255)
                                                      tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGTCCGGGAAAAAAGCCCCGGAAGAGATCGCCGCCG‑GGGAgggaaaa                                                                                           >  4:596741/1‑145 (MQ=255)
                                                      tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑GGAAGGACaaa                                                                                           >  1:827845/1‑150 (MQ=255)
                                                           gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGa                                                                                      <  4:296507/150‑1 (MQ=255)
                                                           gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGGAGGACAAACTgg                                                                                       >  1:940233/1‑149 (MQ=255)
                                                             gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAAc                                                                                   <  3:142996/151‑1 (MQ=255)
                                                                aTGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCt                                                                                <  3:88214/151‑1 (MQ=255)
                                                                aTGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCCAAAACGCCCGAGAAGGGATCGCCGCCG‑TGAAGGACAAACCGGAAAAGc                                                                                 >  4:798341/1‑150 (MQ=255)
                                                                aTGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAAAGc                                                                                 >  1:1120080/1‑150 (MQ=255)
                                                                 tGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTGCCGCCGGCCGGGGAAAACGCCCCGGAAGTGATCGCCCCCG‑GGGAGGGCAAACCggga                                                                                     >  4:13446/1‑143 (MQ=255)
                                                                  gTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCCTCGGTCCCGCCCAAAAGCCCGGGAAGGGGGCGCCGCCGCGGAAGGAAAACTTGGaa                                                                                     >  4:391178/1‑145 (MQ=255)
                                                                  gTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCG‑CGGAGGACAAAACGGAAACGCTg                                                                               >  3:175734/1‑150 (MQ=255)
                                                                    tGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGaa                                                                             <  4:1152878/150‑1 (MQ=255)
                                                                     gCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGaaaa                                                                           <  3:142443/151‑1 (MQ=255)
                                                                      cGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCG‑TGAAGGACAAACTGGAAAAGCTGaaa                                                                            >  1:105023/1‑149 (MQ=255)
                                                                             ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTg                                                                   <  2:186179/151‑1 (MQ=255)
                                                                               aGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCTGCGATCCCGCAAAAAAGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGGAACGCTGAAAAGTAGTCTGcc                                                                 >  1:730119/1‑151 (MQ=255)
                                                                                 aTTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCgg                                                               <  3:97414/151‑1 (MQ=255)
                                                                                 aTTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCgg                                                               <  1:1143064/151‑1 (MQ=255)
                                                                                 aTTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCCGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAAAAGTCTGCCgg                                                               >  2:186341/1‑151 (MQ=255)
                                                                                   tGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGa                                                              <  2:62683/150‑1 (MQ=255)
                                                                                   tGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGa                                                              <  4:525857/150‑1 (MQ=255)
                                                                                    ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAg                                                            <  1:1279923/151‑1 (MQ=255)
                                                                                    ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAg                                                            <  1:632457/151‑1 (MQ=255)
                                                                                    ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAg                                                            <  1:969614/151‑1 (MQ=255)
                                                                                      cccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGc                                                          <  1:758281/151‑1 (MQ=255)
                                                                                      cccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGc                                                          <  1:1066977/151‑1 (MQ=255)
                                                                                          gagaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTgg                                                      >  1:435676/1‑151 (MQ=255)
                                                                                           agaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCCGCGATCCGGCCAAAACGCCCGCGAAGGGGTCGCCGCCG‑GGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCggggg                                                     >  4:1449510/1‑150 (MQ=255)
                                                                                           agaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCCGCAAAAACGCCCGCGAAGTGATCGCCGCCG‑TGAAGGGCAAACTGGAAACGCCGAAAAGTAGTCTGCCGGGAGGCgggg                                                      >  1:345748/1‑150 (MQ=255)
                                                                                               gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCg                                                         <  4:347023/143‑1 (MQ=255)
                                                                                               gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGAATCTGCGATCCCGGAAAAAAACCCCAGAAGTGGTCGCCGGCG‑AGGAGGTAAAAATGGGAACGCTGGAAAGGAGGCTGCCGGGAggggggaacggg                                                 >  1:347049/1‑141 (MQ=255)
                                                                                                cgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCAAAAACGCCCGGGAAGGGAACGCCGCCG‑TGAAGGACAAACTGGAAAAGCTGAAAAGGAGTCTGCCGGGAGGCgggggg                                                    >  4:311055/1‑145 (MQ=255)
                                                                                                cgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGAGCCTGCGATCCGGCGAAAAAGCCCGAGAAGAGATCGCCGCCG‑GGAAAGACAAACTGGAAACGCTGAAAAGGAGTCTGCCGGGAGGCggggg                                                     >  3:30656/1‑145 (MQ=255)
                                                                                                 gcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCCGCCAAAACGCCCCAGAAGTGATCGCCGCCG‑TGGAGGGCAAACTGGGAACGCTGCAAAGTAGGCTGCCGGGAGGCGGGGGGGTAg                                                >  4:856061/1‑150 (MQ=255)
                                                                                                     gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTaca                                           <  1:673968/151‑1 (MQ=255)
                                                                                                     gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTaca                                           <  1:373830/151‑1 (MQ=255)
                                                                                                       gCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGGAGTGATCGCCGCCG‑TGGAGGAAAAACTGGAAACGCTGAAAAAGAGTCTGCCGGAAGGCGTGGAGATAGGTacaa                                          >  1:591753/1‑150 (MQ=255)
                                                                                                         aTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACAt                                       <  1:1407684/151‑1 (MQ=255)
                                                                                                           tGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATa                                      <  1:1020574/150‑1 (MQ=255)
                                                                                                            gCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACg                                    <  1:1201091/151‑1 (MQ=255)
                                                                                                            gCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACg                                    <  4:1313313/151‑1 (MQ=255)
                                                                                                               gAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGGGGAGATAGTTACAACATACGATc                                 >  1:1303482/1‑151 (MQ=255)
                                                                                                               gAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCCATCCGGCAAAAACGCCCGAGGAGGGATCGCCGCCG‑TGAAGGACAAACTGGGAACGCTGAAAAGGAGTCTGCCGGGAGGGGTGGGGATAGTTACAACATa                                      >  2:183173/1‑146 (MQ=255)
                                                                                                                aaCTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCg                                <  4:861184/151‑1 (MQ=255)
                                                                                                                    aaaCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagc                            <  4:636247/151‑1 (MQ=255)
                                                                                                                      aCGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagcc                           >  1:1440691/1‑150 (MQ=255)
                                                                                                                       cGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagccag                         <  1:882474/151‑1 (MQ=255)
                                                                                                                           gaaggcgaagTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATAAGATCGCAGCCAGCTc                      >  1:1143212/1‑150 (MQ=255)
                                                                                                                            aaggcgaagTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCa                     >  1:1084490/1‑150 (MQ=255)
                                                                                                                             aggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCAtt                   <  4:492583/151‑1 (MQ=255)
                                                                                                                             aggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCAtt                   <  1:206881/151‑1 (MQ=255)
                                                                                                                                 gaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGAcc               <  4:364515/151‑1 (MQ=255)
                                                                                                                                 gaagTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCCAAAAAGCCCCGGAAGAGATCGCCGCCG‑CGGAGGACAAACTGGGAAAGCTGAAAAAGAGGCTGCCGGGAGGGGGGGGGGTAGTTACAACATACGATCGCAGCCAGCTCATTGAcc               >  1:103853/1‑151 (MQ=255)
                                                                                                                                    gTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAAGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgc             <  4:1452125/150‑1 (MQ=255)
                                                                                                                                       gcgggcggGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCa         <  4:1142830/151‑1 (MQ=255)
                                                                                                                                       gcgggcggGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCa         <  1:1431513/151‑1 (MQ=255)
                                                                                                                                         gggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATc       <  4:1303370/151‑1 (MQ=255)
                                                                                                                                              gggTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACaa  <  1:235547/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCG‑TGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAA  >  NC_000913/599431‑599723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑