Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,133,3020TG43.3% 180.5 / 15.6 104H219P (CAC→CCC) yghSputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (11/48);  new base G (45/0);  total (56/48)
Fisher's exact test for biased strand distribution p-value = 2.44e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACGGG  >  NC_000913/3133156‑3133450
                                                                                                                                                  |                                                                                                                                                    
gTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTc                                                                                                                                                   <  4:28009/150‑1 (MQ=255)
  aGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGc                                                                                                                                                 <  1:1314075/150‑1 (MQ=255)
  aGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGcc                                                                                                                                                >  1:1301651/1‑151 (MQ=255)
  aGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGcc                                                                                                                                                >  2:97939/1‑151 (MQ=255)
    ttGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGccc                                                                                                                                               >  1:1136655/1‑150 (MQ=255)
     tGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCa                                                                                                                                              <  4:957181/150‑1 (MQ=255)
      gggCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCAt                                                                                                                                             <  4:125325/150‑1 (MQ=255)
        gCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCa                                                                                                                                          <  4:259112/151‑1 (MQ=255)
         cTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTTGGCCATcc                                                                                                                                           >  4:574807/1‑149 (MQ=255)
           gcggcgCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAg                                                                                                                                         <  4:918367/149‑1 (MQ=255)
           gcggcgCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGt                                                                                                                                        <  1:599341/150‑1 (MQ=255)
             ggcgCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCa                                                                                                                                     <  4:1332045/151‑1 (MQ=255)
             ggcgCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCa                                                                                                                                     <  1:900243/151‑1 (MQ=255)
               cgcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTcccggt                                                                                                                                       >  1:478258/1‑143 (MQ=255)
                gcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCCGTTCAAgg                                                                                                                                  >  1:305776/1‑151 (MQ=255)
                gcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAGTTCAAgg                                                                                                                                  >  1:831557/1‑151 (MQ=255)
                   gTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGTGCCCCTCCAGTTCaa                                                                                                                                    >  4:223638/1‑146 (MQ=255)
                   gTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCCGTTCAAGGaac                                                                                                                               >  4:168566/1‑149 (MQ=255)
                      gTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCtt                                                                                                                             <  1:726919/150‑1 (MQ=255)
                        aCTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTgc                                                                                                                          <  1:594466/151‑1 (MQ=255)
                         cTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCCCCCATCCCGTTTAAGGATCttt                                                                                                                            >  4:39867/1‑148 (MQ=255)
                            ttAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTTCGcc                                                                                                                       >  1:796157/1‑150 (MQ=255)
                              aaCAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTTAAAGATCTTTGCGCCCtt                                                                                                                    >  1:1214228/1‑151 (MQ=255)
                              aaCAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGGTCAAGGATCTTTTCGCCCtt                                                                                                                    >  1:887970/1‑151 (MQ=255)
                                cAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGa                                                                                                                  <  3:188025/151‑1 (MQ=255)
                                     tgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTCAAGGATCTTTGCGCCCTTTAAt                                                                                                                >  1:873446/1‑148 (MQ=255)
                                      gagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCa                                                                                                            <  4:1149151/151‑1 (MQ=255)
                                      gagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCCAGGATCTTTTCGCCCTTGAATGt                                                                                                              >  1:120729/1‑149 (MQ=255)
                                        gtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATACAGTTCAAGGATCTTTGCGCCATTGAATGTCa                                                                                                            >  1:746493/1‑149 (MQ=255)
                                           tgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTg                                                                                                        <  2:121397/150‑1 (MQ=255)
                                           tgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTCAAGGATCTTTTCGCCCTTGAATGTCAGt                                                                                                          >  1:797663/1‑148 (MQ=255)
                                            gTAGTGCATCCATTCGTTCAATCCTCCCCCATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGCGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTgc                                                                                                       <  4:423284/150‑1 (MQ=255)
                                              aGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAGGTCAAGGATCTTTTCGCCCTTGAATGTCAGt                                                                                                          >  4:342785/1‑145 (MQ=255)
                                                tGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCCGTTCAAGGATCTTTGCGCCATTGAATGTTAGTTggggcg                                                                                                   >  1:1189353/1‑150 (MQ=255)
                                                tGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCCGTTCAAAGATCTTTTCGCCATTTAATGTCCGTTggggcg                                                                                                   >  1:1248588/1‑150 (MQ=255)
                                                   aTCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGGGGCCATCCAGTTCAAGGGTCTTTGGGCCATTGAATGACAGTTgcggaggaa                                                                                                >  1:415763/1‑145 (MQ=255)
                                                    tCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCCTCCAGTTTGAAGAGTTTTGAGCCTTTTTATGTCAGTTGCGGCGGaa                                                                                                >  1:668925/1‑149 (MQ=255)
                                                    tCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTCTCGGAACTTTTTGCCCTTGAATGTTAGGTGCGGCGGAAc                                                                                               >  4:1193531/1‑150 (MQ=255)
                                                     ccATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGGTCAAGGATCTTTTCGCCCTTGAATGGCAGTTGCGGCGGAACgg                                                                                             >  1:303753/1‑151 (MQ=255)
                                                      cATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGc                                                                                            <  1:337572/151‑1 (MQ=255)
                                                         tCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGTTCAAGGGTCTTTTCGCCATTGGATGTCAGTTGCGGCGGGACGGCGAt                                                                                         >  4:574261/1‑151 (MQ=255)
                                                           gTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGAttt                                                                                       <  4:809659/151‑1 (MQ=255)
                                                              aaaTCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGTTCCAGGATCTTTGCGCCATTTAATGTCAGTTGCGGGGGAACGGCGGgttttt                                                                                    >  4:1374757/1‑151 (MQ=255)
                                                              aaaTCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAGGTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGAtttttt                                                                                    >  2:133530/1‑151 (MQ=255)
                                                               aaTCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGGTCAAAGATCTTTTCGCCCTTTAATGTCAGTTGCGGCGTAACGGCGGttttttt                                                                                   >  1:778088/1‑150 (MQ=255)
                                                                  catcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGGGGCCCCCCCAGTTCAAGGATCTTTTCGCCATTTAATGTCAGTTGCGGGGCAACGGCGGtttttt                                                                                    >  4:703892/1‑147 (MQ=255)
                                                                   atcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGt                                                                               <  3:13391/151‑1 (MQ=255)
                                                                   atcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTTCTGt                                                                               >  1:686781/1‑151 (MQ=255)
                                                                     ccccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTaa                                                                             <  1:1101431/151‑1 (MQ=255)
                                                                     ccccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTa                                                                              <  4:783075/150‑1 (MQ=255)
                                                                     ccccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTa                                                                              <  1:331403/150‑1 (MQ=255)
                                                                     ccccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAAGAACTTTTCGCCCTTTAATGTCAGTTGGGGGGGAACGGGGGGTTTTTTCttt                                                                               >  4:1117492/1‑147 (MQ=255)
                                                                        cTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTaa                                                                             <  1:1425788/148‑1 (MQ=255)
                                                                        cTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGTGCCCCTCCCGTTTAAGGATTTTTTCGCCATTGAATGTCAGTTGCGGCGGAAAGGCGGttttttttttttaa                                                                            >  4:1425657/1‑141 (MQ=255)
                                                                           tCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGGCGCCCATCCAGATAAAGGAGCTTTGAGGCCTTGAAAGTCAGGTTTGGGGGGACGGGGAGTTTTTCCTGtta                                                                             >  4:457047/1‑143 (MQ=255)
                                                                           tCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGGCCATCCAGTTCCAGGATATTTTTGCCAACTAATGTGAGTGGCGGGGGAACGGGGGTTTTTTTCTCTAACga                                                                          >  4:1065059/1‑147 (MQ=255)
                                                                             cATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCa                                                                     <  1:1242897/151‑1 (MQ=255)
                                                                              aTTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAGTTCAAGGATCTTTTCGCCCTTGAATGTCAGTTGCGGCGGAACGGCGATTTTTTTCTGTAAAGCTGcc                                                                      >  1:1307556/1‑149 (MQ=255)
                                                                                  tGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCATTTTAAGGGTCTTTTCGCCACTGAATATCCGTTTGGGGGGGAAGGCGCGTTTTTTTTTTAAAGCTGCCCGtt                                                                  >  4:1102597/1‑149 (MQ=255)
                                                                                   ggTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGGCCATCCCGCTCAAGGATCTTTGCGCCCTTGAATGTCTATTGTGGCGGGACGGCGGGTTTTTTCttttacaccgcc                                                                      >  4:1094760/1‑133 (MQ=255)
                                                                                   ggTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTtggt                                                               <  3:166311/151‑1 (MQ=255)
                                                                                   ggTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTtggt                                                               <  4:656526/151‑1 (MQ=255)
                                                                                         cAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAg                                                         <  4:685269/151‑1 (MQ=255)
                                                                                         cAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAg                                                         <  4:193025/151‑1 (MQ=255)
                                                                                          aGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTCAAGGATCTTTTCGCCCTTGAATGGCAGTTGCGGGGGAAAGGGGATTTTTTTCCTTAAAGCCGCCCGTTGGGGGTCCgg                                                        >  2:181411/1‑151 (MQ=255)
                                                                                                gcgcGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTAcg                                                  <  1:1286794/151‑1 (MQ=255)
                                                                                                 cgcgGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCCTCCAGTTCAAGGATCTTTGCGCCCTTGGATGTCAGTTGCGGGGTAACGGGGGTTTTTTTCTGTTAAGCTGCCCGGtgggggggggggtttcg                                                  >  1:1352519/1‑139 (MQ=255)
                                                                                                 cgcgGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTTCGCCATTTAATGTCAGTTGCGGCGGAAAGGCGGTTTTTTTCTGTAAAGCTGCCAGTTGGTGGGCCGGTTTTcg                                                  >  1:800356/1‑150 (MQ=255)
                                                                                                  gcggcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAGTTCCAAGAGCTTTTCGCCATTGAATGTCAGTTTCGGCGGAAAGGCGGTTTTTTTCTTGAACGCCGCCCGTTGGTGGTGAGGGTTTcg                                                  >  4:162376/1‑149 (MQ=255)
                                                                                                             tGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGt                                      <  1:1400800/150‑1 (MQ=255)
                                                                                                              gcgcGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGttt                                    <  1:802921/151‑1 (MQ=255)
                                                                                                              gcgcGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGGTCAAGGATCTTTGCGCCCTTTAATGTCAGTTGCGGCGGAACGGCGGTTTTTTTCTTTTACGCTGCCCGGTGGGGGGGAGGGTTTCGCGCAAACGCGGgtt                                    >  4:1003574/1‑151 (MQ=255)
                                                                                                               cgcgTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTg                                   <  1:261418/151‑1 (MQ=255)
                                                                                                                gcgTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGt                                  <  1:279659/151‑1 (MQ=255)
                                                                                                                  gTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCCCCAGTTCAAGGATCTTTTCGCCATTGAATGTCAGTTGCGGGGGAAAGGCGGTTTTTTTTTTTAAAGCTGCCAGGTGGGGGGGAGGGTTTCGCGCCAAAGCGGGGTGttt                                >  4:1214129/1‑150 (MQ=255)
                                                                                                                    ggTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTTAAGGAGCTTTGCGCCATTGGATGTCAGGTGCGGCGTAACGGGGGTTTTTTTCTGTTACGCTGCCCGTTGGTGGGCAGGGTTTAGCGCCAACGCGGgttgtt                                 >  4:469384/2‑148 (MQ=255)
                                                                                                                    aGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTtcat                              <  1:1475225/151‑1 (MQ=255)
                                                                                                                    aGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTtca                               <  4:1368570/150‑1 (MQ=255)
                                                                                                                    aGTGACGCTTGCAGAATTTCATCGGCGGGGTGGTGCCCATCCAGTTCAAGGATCTTTTGGCCATTGGATGTCAGTTGCGGGGTAAAGGCGAGTTTTTTCTGTTAAGCTGCCCGGTGGTGGGCAGGGTTACGCGCAAAAGCCGgttgtt                                 >  3:28437/1‑148 (MQ=255)
                                                                                                                    aGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCATTTCAAGGGGCTTTGTGCCATTTATTGTGATTTTCGGCGTGACGGCGGTTTTTTTCTTTTACGACGCCAATTTGGGGGGAGATGTTTGCGCAAACACggggttgt                                 >  4:1442665/1‑142 (MQ=255)
                                                                                                                    aGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCCCCCAACCAGTTCAAAGGTCTTTGCGCCCTTGCATGGCTGGTTCGGCGTTACGGGGATTTTTTCCTGTAACGCTGCCCGGTGGGGGGCAGGATTACGCGCCAAAGCGGGTttttttt                              >  2:139724/1‑148 (MQ=255)
                                                                                                                    aGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAGTTCAAGGATCTTTTTGCCATTGAATGTCAGGTGCGGCGTAAAGGCGGTTTTTTTCTGTTACGCTGCCCGGTGGTGGGCAGGTTTTAGCGCAAACGCGGGTTGTttat                              >  3:148233/1‑151 (MQ=255)
                                                                                                                     gTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTtcatc                             <  4:505680/151‑1 (MQ=255)
                                                                                                                         cGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATg                         <  4:739471/151‑1 (MQ=255)
                                                                                                                          gCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGc                        <  2:133075/151‑1 (MQ=255)
                                                                                                                          gCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGc                        <  4:68550/151‑1 (MQ=255)
                                                                                                                          gCTTGCAGAATTTCATCGGCGGGGGGTGGCCCCTCCCGTTTAAGGGTCTTTTCGCCCTTGAATGTCGGGTGCGGCGGTAAGGCGGTTTTTTTCTGTAAAGTTGCCCGTTGGGGGTGGGGGTTAAGCGGAAAAGCGGGTTGTTTATTaa                           >  4:848111/1‑148 (MQ=255)
                                                                                                                            ttGCAGAATTTCATCGGCGGGGGGTTGCCCCTCCAGCTCAAGGATCTTTGCGCCATTGAATGTCAGGTGCGGCGCAACGGCGAGTTTTTTCTCTAACGCTGCCCGGTGGGGGGCCGGGTTACGCGCAAAAGCGGTTTTTTCCTCAATAcc                       >  4:47953/1‑150 (MQ=255)
                                                                                                                            ttGCAGAATTTCATCGGCGGGGGGTCCCCCATCCAGTTCAAAGATCTTTTCGCCATTGAATGTTAGTTGCGGGGTAAAGGCGGTTTTTTTCTTTAACGCTGCCAGTTGGTGGTTAGGGTTACGCGCAAACGCGGGTTGTTTATTAATGcc                       >  1:1365939/1‑150 (MQ=255)
                                                                                                                                 gAATTTCATCGGCGGGGGGTCGCCCATCCAGTTTAAAGATCTTTGTGCCATTGAATGTCAGTTGCGGCGTAAAGGCGATTTTTTTCTGTAAAGCTGCCCGGTGGGGGGGAGGGTTTCGCGCAAACGCGGGTTGTTTCTCAATGCCAAGa                   >  4:635520/1‑149 (MQ=255)
                                                                                                                                   aTTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGaa               <  1:71910/151‑1 (MQ=255)
                                                                                                                                       cATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATca            <  4:58851/150‑1 (MQ=255)
                                                                                                                                        aTCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATcaac          <  4:1451417/151‑1 (MQ=255)
                                                                                                                                          cggcggGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATcaaca         >  1:792563/1‑150 (MQ=255)
                                                                                                                                          cggcggGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATcaaca         <  1:68476/150‑1 (MQ=255)
                                                                                                                                           ggcggGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATcaacaa        >  1:770904/1‑150 (MQ=255)
                                                                                                                                              ggggTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACg    >  4:36805/1‑151 (MQ=255)
                                                                                                                                               gggTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACgg   <  4:638444/151‑1 (MQ=255)
                                                                                                                                               gggTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACgg   <  1:1033836/151‑1 (MQ=255)
                                                                                                                                                ggTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACggg  <  4:1248482/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACGGG  >  NC_000913/3133156‑3133450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑