Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	51.1%	50.1 / 136.2	92	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (6/39);  new base T (47/0);  total (53/39)
Fisher's exact test for biased strand distribution p-value = 5.71e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.54e-08

ATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206291‑3206584                                                                                                                                                   |                                                                                                                                                        aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACa                                                                                                                                                   <  4:691053/151‑1 (MQ=255)  tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTcacaca                                                                                                                                                   >  1:387269/1‑150 (MQ=255)   ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCCGACaa                                                                                                                                                  >  1:1396318/1‑150 (MQ=255)      gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATAc                                                                                                                                              <  4:722599/151‑1 (MQ=255)       cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACa                                                                                                                                             <  4:1141147/151‑1 (MQ=255)        aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGA‑ATTTTTCAGACAAAAACa                                                                                                                                             >  4:154497/1‑150 (MQ=255)        aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCCGACAAATa                                                                                                                                               >  1:495012/1‑148 (MQ=255)         aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACaaa                                                                                                                                           <  1:216890/151‑1 (MQ=255)         aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATAGTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAATTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACaaa                                                                                                                                           <  4:770779/151‑1 (MQ=255)           aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCCGacaaaaacaaaa                                                                                                                                          >  3:34334/1‑150 (MQ=255)           aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACaaaaa                                                                                                                                         <  4:787437/151‑1 (MQ=255)             aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCCGAaaaaaaaaaaaa                                                                                                                                         >  1:1025430/1‑149 (MQ=255)               tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGCGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTg                                                                                                                                      <  2:168167/150‑1 (MQ=255)                gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGACAAATACaaaaa                                                                                                                                         >  1:230337/1‑146 (MQ=255)                 ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                   <  1:813030/151‑1 (MQ=255)                  cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGt                                                                                                                                  <  4:912818/151‑1 (MQ=255)                  cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑ATTTTTCCGACAAATACAAAAAATgg                                                                                                                                     >  1:1033402/1‑148 (MQ=255)                   aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCACAcaaaaacaaaaa                                                                                                                                         >  4:1271512/1‑143 (MQ=255)                   aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGtt                                                                                                                                 <  1:811562/151‑1 (MQ=255)                    ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGA‑TTTTTTCAAACAAAAACAAAAAATGGAggt                                                                                                                                 >  4:467742/1‑148 (MQ=255)                      cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑ATTTTTCAGACAAAAACAAAAAAATGAGTTgg                                                                                                                               >  4:29528/1‑149 (MQ=255)                       ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCa                                                                                                                            <  3:173483/151‑1 (MQ=255)                          gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCatga                                                                                                                         <  1:922862/151‑1 (MQ=255)                           ctctGGCGCAATATTCATTCTTATTTTCGCGTCGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCACAcaaaaacaaaaaa                                                                                                                                        >  4:360457/1‑135 (MQ=255)                             ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCatgat                                                                                                                        <  4:643952/149‑1 (MQ=255)                                gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACACATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCCGACAaaaaaaaaaaaatgaggtggc                                                                                                                              >  1:1308780/1‑130 (MQ=255)                                 cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGAAAAAAAAAAAAAATTGAGGTG‑CCATGATGAggg                                                                                                                   >  1:982840/1‑148 (MQ=255)                                   cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGAAAAATAAAAAAAATAGAGTTGTCCAT‑ATGAGCgga                                                                                                                 >  4:1422752/1‑148 (MQ=255)                                    aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTTAAAAAAAAAAAAAAACAGGGGGTG‑CCCTGATGAGCgga                                                                                                                 >  3:104064/1‑147 (MQ=255)                                     atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGACAAAAACAAAAAATGGGGGTG‑CCATGATGAGCGaaa                                                                                                                >  1:323973/1‑149 (MQ=255)                                     atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGt                                                                                                              <  1:197960/151‑1 (MQ=255)                                       attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGtaa                                                                                                            <  1:1305241/151‑1 (MQ=255)                                         tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑TGA‑TTTTTTCCGAAAAAAAAAAAAAATGGAGTTG‑CCatgattggcggaaagaa                                                                                                            >  1:1449063/1‑137 (MQ=255)                                         tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGtaat                                                                                                           <  1:1377472/150‑1 (MQ=255)                                         tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGtaat                                                                                                           <  4:1106630/150‑1 (MQ=255)                                          cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGtaataa                                                                                                         <  4:363154/151‑1 (MQ=255)                                                tATTTTCGCGTTGATTCACGAACTGTTAATTACACATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑a                                                                                                     <  4:521252/148‑1 (MQ=255)                                                  ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGAAAAAAACAAAAAATGGAGTTG‑CCATGATGAGCGgaaagaaaaaacaa                                                                                                     >  1:1038098/1‑136 (MQ=255)                                                    ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTTCCAAAAATACAAAAACTTGAGGTG‑CCCTGGTGAGCGGAAGAAATAAAG‑AACa                                                                                                  >  1:1468732/1‑147 (MQ=255)                                                    ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGACAAATAAAAAAAATGGAGGTG‑GCATGATGAGCGAAAGGAATAAac‑aacaag                                                                                                >  1:112841/1‑147 (MQ=255)                                                            gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATaa                                                                                      <  1:243626/151‑1 (MQ=255)                                                             aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAg                                                                                     <  4:307588/151‑1 (MQ=255)                                                              ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGA‑GGA‑ATTTTTCAGACAAATAAAAAAAAAGGGGGTT‑GCATTATTAGCGAAAGGAATAAAC‑AAAAAGCAGGGAATa                                                                                       >  1:764844/1‑148 (MQ=255)                                                               tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGA‑TTTTTTCAGACAAAAAAAAAAACTGGAGGTG‑CCATGATGAGCGGAAGAAATAAGC‑AACAAGCAGGGAATaa                                                                                      >  1:1195054/1‑148 (MQ=255)                                                               tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGACAAAAACAAAAACTGGAGTTG‑CCATGATGAGGGGAAGTAATAAAC‑AAAAAGCAGGGAaaaa                                                                                      >  1:1413709/1‑148 (MQ=255)                                                               tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGACAAAAAAAAAAAATGGAGGTG‑CCATGATGAGCGAAAGTAATAAAC‑AAAAAGCAGGGAAAAAGtt                                                                                   >  1:416596/1‑151 (MQ=255)                                                                cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGtt                                                                                   <  4:784802/150‑1 (MQ=255)                                                                 aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAA‑TTTTTCCA‑ACAAAAAAAAAAAATTGAGGTT‑TCATGGTGGGGGGAAATAATAAAA‑AAAAAGAAGGGAATaa                                                                                      >  4:86148/1‑146 (MQ=255)                                                                   gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACa                                                                               <  1:206712/151‑1 (MQ=255)                                                                      cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGC‑GAA‑TTTTTTTAAACAAAAACAAAAAATGGAGTTT‑GCATGATGAGGGGAAGAAATAAAC‑AAAAAGCAGTGAAAAAGTTGaaa                                                                               >  4:555905/1‑146 (MQ=255)                                                                       tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGACCAAAAAAAAAAATGGAGTTG‑CCCTGGTGGGGGGAAGAAAAAAAC‑AAAAAGCAGGGAATAAAGTGACa                                                                               >  1:50072/1‑147 (MQ=255)                                                                       tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑ATTTTTCAGACAAAAACAAAAAATTGGGTTG‑CCCTGATGAGCGAAAGTAAAAAGC‑AAAAGGGAGGGAATAAGATGACa                                                                               >  1:177154/1‑147 (MQ=255)                                                                           aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTg                                                                        <  2:44468/150‑1 (MQ=255)                                                                            aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                      <  1:1126753/151‑1 (MQ=255)                                                                             ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGACAAATACAAAAAATGGAGTTG‑CCATGATGAGCGAAAAGAAAAAAC‑AACAAGCAGTGAAAAAATTGAAAgagaat                                                                         >  1:479688/1‑145 (MQ=255)                                                                                cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGACAAAAACAAAAAATGGAGTTG‑CCATGATGAGCGAAAGTAAAAAAC‑AACAAGCAGTGAATAAGTTGACAGAGATTGt                                                                       >  1:306286/1‑146 (MQ=255)                                                                                   atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAAc                                                                <  1:681739/150‑1 (MQ=255)                                                                                   atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACt                                                               <  4:913143/151‑1 (MQ=255)                                                                                    ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑ATTTTTCCTACAAATACAAAAAACTGAGTTG‑CCCTGATGAGTGCAAATAATAAGC‑AACAAACAGTGAATAAATTGACAGAGAGTGTTGCTaa                                                                 >  2:69573/1‑148 (MQ=255)                                                                                     tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAAAAAAAAATAAAAAATCGAATTG‑CCATAAAGACGGAAAATAAAAAAC‑AACAGGGAGAGAAAAAATTGAAAGAAATTTTCGCGaaactt                                                             >  1:1320702/1‑147 (MQ=255)                                                                                     tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑ATTTTTCACAAAAAAACAAAAAATGGAGGTG‑GCATGATGAGCGGAAAGAATAAAA‑AAAAAGCAGTGAATAAAATGACAGAGAATTTTGCTAACttt                                                             >  1:616812/1‑151 (MQ=255)                                                                                       aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑ATTTTTCAAACAAATAAAAAAAAAGGAGGTG‑GCATGATGGGAGAAAGAAAAAAGA‑AACAAGGCGGGAATAAAGTGACAGAGAATTTCGCTCACttt                                                             >  1:394231/1‑149 (MQ=255)                                                                                         ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGA‑ATTTTTCAAACAAAAAAAAAAAATTGAGGTT‑TTATGTTGGGGGAAAGGAATAAGC‑AAAAAGGAGGGGATAAGATGAAAGAGAATGTCGCTAAAtttttc                                                          >  4:490612/1‑147 (MQ=255)                                                                                              cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAAACAAAAAAAAAAAATGGAGGTG‑GCATGGTGGGGGGAAGTAATAAAC‑AAAAAGCAGTGAATAAATTGACAGAGATTGTCGCTAAATTTACCGcc                                                       >  4:1024461/1‑148 (MQ=255)                                                                                               aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCCGACAAATACAAAAAATGGAGGTG‑CCCTGATTAGGGAAAGTAATAAGG‑AAAAGGGAGAGAAAAAAGTGAAAGAAAATGTCGCCAACCTTACCCCCAt                                                     >  1:1331336/1‑149 (MQ=255)                                                                                               aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGACAAAAAAAAAAAATGGAGGTG‑CCATGATGAGCGAAAGAAATAAAA‑AAAAGGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACcccc                                                       >  1:524390/1‑147 (MQ=255)                                                                                               aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATg                                                    <  4:1290489/150‑1 (MQ=255)                                                                                                 aaaaTATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAtt                                                 <  1:1241533/151‑1 (MQ=255)                                                                                                    atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGA‑TTTTTTCCGCCAAAAACAAAAAATGGAGGTG‑CCATGATGAGCGAAAGGAATAAAA‑AACAGGCAGGGAAAAAATTTTCAGAGATTGTCCCTAACTTTATCCCCACGAttt                                                >  1:910371/1‑149 (MQ=255)                                                                                                    atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GCA‑ATTTTTCAGACAAATAAAAAAAATGGAGTTG‑TGCTTGTGAGGGCAAGAAATAAGC‑AACAGGGAGAGAAAAAAATGGGAGAGATTGTCGCTAACCTTTACGCCATGAatttt                                              >  4:73547/1‑149 (MQ=255)                                                                                                      ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGA‑TTTTTTCCGACAAAAACAAAAACTTGGGGTG‑GCATGGTGTGCGAAAGGAAAAAACAAAAAGGCGGGGAA‑AAGTTGACAGAGATTTTCGCCAAATTTTCCGCCCTGAttt                                                >  1:45320/1‑147 (MQ=255)                                                                                                        aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAAACAAAAAAAAAAAAAGGAGGTG‑GCATGATGGGGGGAAGTAATAAAC‑AACAAGCAGGGAATAAAATGACAGAGATTGTTGCTCACTTTAACGCCCTGATTTTTAccc                                          >  4:1387946/1‑151 (MQ=255)                                                                                                          aaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGACAAAAAAAAAAAATGGGGGTG‑GCATGGTGGGGGGAAGGAATAAAA‑AACAAGGAGAGAAAAAATTGACAGAGAATGTCGCTAACTTTTCCGCCATGATTTTTAccc                                          >  4:527628/1‑149 (MQ=255)                                                                                                           aCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCCAACAAAAAAAAAAAATGGAGGTG‑CCATGATGAGGGGAAGTAATAAAA‑AAAAAGCCGTGAAAAAATTGACAGAGATTTTCGCTAACTTTTCCGCCATGATTTTTAccc                                          >  1:1445894/1‑148 (MQ=255)                                                                                                            ccACATTTTTAGTGGTAGTTTGTGGCGTT‑GAA‑TTTTTCCAGACAAAAACAAAAACTGGAGTTG‑CCATGATGAGCAAAAGTAATAAGC‑AACGGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                       <  1:1331065/150‑1 (MQ=255)                                                                                                            ccACATTTTTAGTGGTAGTTTGTGGCGGG‑GAA‑TTTTTTCCGACAAAAAAAAAAAACGGAGTTG‑TCATGATGGGGGGAAAGAATAAAC‑AACAAGCAGTGAATAAAGTGAAAGGGATTTTCGCGAACTTTACCCCCATGATTTTTAccc                                          >  4:1106896/1‑147 (MQ=255)                                                                                                             cacaTTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGACAAAAACAAAAAATGGAGTTG‑CCCTGATGGGCGGAAGGAATAAGC‑AAAAAGCAGGGAATAAAGTGACAGAGATTGTCGCTAACTTTAACGCCATGATTTCTACCCGaa                                       >  1:459555/1‑149 (MQ=255)                                                                                                               caTTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTTCAGACAAAAACAAAAAATGGAGTTG‑CCATGATGAGCGAAAGTAATAAAC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCCAATGcc                                   >  1:336602/1‑151 (MQ=255)                                                                                                                  ttttAGTGGTAGTTTGTGGCGGC‑GAATTTTTTCAAGCCAATAAAAAAAACCGGAGTTA‑CCGGGTTGGGGGAAATGAAAAAG‑‑AACAAGGAGGGGATAAGTTGAGAGAAATTGGCGCTAACTTTTCCGCCCTGGTTTTTTcccccaagcc                                   >  1:739137/1‑141 (MQ=255)                                                                                                                     tAGGGGTATTTTGTGGCGGT‑AAA‑TTTTTCCGAACAAATCAAAAGACTGGATTTT‑CCTAAATGGGAGAAAGTAATAAGC‑AACAGAGGTTGAATAGATTGACAGGGTTTGTCGGTAATTTTACCGCCATGATTTCTACCCGAATGCCtg                                 <  1:708801/147‑1 (MQ=255)                                                                                                                              tttGGGGCGGT‑GAA‑TTTTTCCAGAAAAATACAAAACCGGGAGTGG‑CCATGATGAGCAAAAGTAATAAGC‑AACAGGCAGTAAATAAGTGGACAGAGATGGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg                      <  4:1192247/149‑1 (MQ=255)                                                                                                                                tgtgGCGGT‑GAA‑TTTTACCAGAAAAAAAAAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTAAATAGGTTGACAGAGTTTGTCGTTATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                   <  4:922786/150‑1 (MQ=255)                                                                                                                                    gCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt              <  1:505298/151‑1 (MQ=255)                                                                                                                                      ggT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa             >  1:912982/1‑150 (MQ=255)                                                                                                                                      ggT‑GAA‑TTTTTCCAGACAAATAAAAAAACTGGAGTTG‑CCATGATGAGAAAAAGAAATAAGC‑AACAGGCAGTGAATAAGTGGACAGAGATTGTCGTTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa            <  3:19963/151‑1 (MQ=255)                                                                                                                                      ggT‑AAA‑TTTTTCCAGAAAAATAAAAAAACTGGAGTTG‑CCATGAGGAGCAAAAGTAAAAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt              <  4:617224/149‑1 (MQ=255)                                                                                                                                          gAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc         >  4:837433/1‑151 (MQ=255)                                                                                                                                           aa‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc         >  4:537908/1‑150 (MQ=255)                                                                                                                                           aa‑TTTCTCCAGACAAATAAAAAACCTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa           <  1:740601/148‑1 (MQ=255)                                                                                                                                               ttttCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGGGAAAGTAATAAGA‑AACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc      <  1:1218615/150‑1 (MQ=255)                                                                                                                                                tttCCAGAAAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt     <  1:1011282/150‑1 (MQ=255)                                                                                                                                                  tCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGATGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCATGATGACGTGGTGGATAAACTAAAACAGCTaa   >  4:1166670/1‑150 (MQ=255)                                                                                                                                                   ccAGAAAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa  <  1:576159/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                        ATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑TTTTTCCAGACAAATACAAAAACTGGAGTTG‑CCATGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206291‑3206584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑