Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8900CA52.1% 48.9 / 114.0 71L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (29/5);  new base A (0/37);  total (29/42)
Fisher's exact test for biased strand distribution p-value = 4.06e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.72e-10

CTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAAT  >  NC_000913/357740‑358036
                                                                                                                                                         |                                                                                                                                                      
cTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaac                                                                                                                                                        >  1:1453369/1‑151 (MQ=255)
  gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaaca                                                                                                                                                       <  1:1055590/150‑1 (MQ=255)
     aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc                                                                                                                                                   <  4:311546/151‑1 (MQ=255)
      aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAATTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAcc                                                                                                                                                  >  1:960258/1‑151 (MQ=255)
        cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccg                                                                                                                                                 <  4:842140/150‑1 (MQ=255)
          tAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgccg                                                                                                                                              >  1:80790/1‑151 (MQ=255)
           aGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGt                                                                                                                                             >  1:841046/1‑151 (MQ=255)
             cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtc                                                                                                                                           >  4:1024163/1‑151 (MQ=255)
             cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtc                                                                                                                                           >  2:174553/1‑151 (MQ=255)
                       aaTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagt                                                                                                                                   >  4:709733/1‑149 (MQ=255)
                         ttCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTg                                                                                                                               >  4:1091658/1‑151 (MQ=255)
                           ccccccGCAGCCGTGTGTCGGGGCCTCCCGTGTTTCTTTCCCCCCAACACCCGCGCTTTTATCAAACGCCTTGTAAAAAATTCCCTCCGGGGCGGTTGTCGGCCGGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCagtag                                                                                                                                 <  3:180308/143‑1 (MQ=255)
                            cAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCg                                                                                                                            >  4:643699/1‑151 (MQ=255)
                             aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                                           >  1:1248575/1‑151 (MQ=255)
                             aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                                           >  4:265081/1‑151 (MQ=255)
                               ccGCAGCCTTGTGTCGGCGCCTCCCGTTTTTCTTTCCCGCAAACACCCGCGTTTTTACCCACCGCCGTGTAAAAAAATGCCTCCGCGGCGGTTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                                          <  4:992550/150‑1 (MQ=255)
                               ccGCAGCCTTGTGTCAGCGGCTCCCGTGTTTCTTTCCCCCCAACACCCGCGCTTTTCCCCACCCCCTTGTGCAAAAATCCCTCCGGGGGGGCTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCg                                                                                                                            <  1:983614/148‑1 (MQ=255)
                               ccGCAGCCCTGTGTCAGCGGCTACCGTGTTTCTTTCCCGCCAACACCCGCGCTTTTACCCAACGCCATGTGAAAAAATCCCTGCGCGGCGGCTGTCTCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                                           <  1:994694/149‑1 (MQ=255)
                               ccGCAGCCCTGTGCCGGCGCCCCCCGTGTTTCTTTCCCCCAAACACCCGCGTTTTTACCCACCCCCATGTAAAAAATTGCCTCCGGGGGGGCGGCCGCCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                                          <  4:292756/150‑1 (MQ=255)
                               ccGCAGACTTGTGTCGGGGCCTCCCGTGATTCTTTCCCCCCAACACCCGCCCTTTTACCCAACGCCTTGTGAAAAATTCCTTCCGGGGCGGTTGTCGGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                                           <  1:56401/149‑1 (MQ=255)
                                cGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggg                                                                                                                        >  4:496844/1‑150 (MQ=255)
                                cGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                         >  1:372179/1‑150 (MQ=255)
                                  cagccgtgtgCCAGCGCCTCCCGTGATTCTTCCCCCCCAACACCCGCGCTTTTCCCCAACGCCATGTGAAAAAATGCCTCCGGGGCGTCTGTCTGCCAGTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCaa                                                                                                                      <  4:1232255/145‑1 (MQ=255)
                                    gcctgtgcccAGCGGCAACCGTGTTTCTTTCCCGCAAACACCCGCACTTTTACCAAACGCCAGGTAAAAAATGCCCGCCGGGGGGGCTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCaa                                                                                                                      <  4:1202687/140‑1 (MQ=255)
                                    gcccTGTGCCAGGGCCTCCCGTGTTTCTTTCCCCCCAACACCCGCGCTTTTACCCACCGCCTTGTGAAAAAATCCTTCCGGGGGGGCTGTCTGCCATTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAaga                                                                                                                    <  1:156131/149‑1 (MQ=255)
                                     aCCTGTGTCAGCGGCTCCCGTGATTCATTTCCCCCAACACCCGCGATTTTACCCAACCCCATGTCAAAAAATGCCTTCGCGGGGGCTGCCTCCCAGTTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                                           <  1:161173/143‑1 (MQ=255)
                                      ccttgtccggCGGCTCCCGTGATTCATTCCCCCCAACACCCGCGCTTTTACCCACCCCCTTGTGCAAAATTCCCTCCGCGGCGGTTGCCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAag                                                                                                                     <  1:569720/139‑1 (MQ=255)
                                       cTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggggggag                                                                                                                   >  4:15904/1‑143 (MQ=255)
                                       cTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                         >  1:878203/1‑143 (MQ=255)
                                        tgtgTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggg                                                                                                                        >  3:31396/1‑142 (MQ=255)
                                          ttccAGCGGCTCCGTTGATTATTTCCCGCCAACACCCGCGCATTTACCCACCCCCTTGTGAAAAATCGCCTCGGGGGGGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCa                                                                                                                       <  1:882375/139‑1 (MQ=255)
                                          tgccAGCGCCTCCCGTAATTCTTTCCCCCCAACACCCGCCCTTTTACCCACCCCAATGTGAAAAAATGCCTCCGCGGCGGCTGTCTCCCACTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAt                                                                                                               <  1:924682/147‑1 (MQ=255)
                                           gtCGGCGGCTCCCGTTATTCTTTCCCGCCAACCCCCGCGCATTTACCCACCGCCATGTGCAAAAAGCCCTCCGGGGGGGCTTTCTGCCGGTTATTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAg                                                                                                             <  4:221312/151‑1 (MQ=255)
                                             cAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggcagcgcaaa                                                                                                               >  4:297361/1‑137 (MQ=255)
                                               gCGGCTCCCGTGTTTCTTTCCCGCCACCACCCGCGCTTTTACCCACCGCCTTGTAAAAAAATGCTTCGGGGGCGGCTGTCTCCGAGTTTTTTTCCCCCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAg                                                                                                             <  1:618516/147‑1 (MQ=255)
                                               gCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAAAGAAAAAACttt                                                                                                        >  1:1373059/1‑151 (MQ=255)
                                               gCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGgaaaaagatt                                                                                                         >  4:745333/1‑144 (MQ=255)
                                                 ggCTCCCGTGATTCATTCCCGCCAACACCCGCGCTTTTACCCACCGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAg                                                                                                             <  1:52958/145‑1 (MQ=25)
                                                  gCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAAATTTaa                                                                                                      >  1:903117/1‑150 (MQ=255)
                                                     aCCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAAAGAAAAAGCCTTTAAAcc                                                                                                   >  1:77570/1‑151 (MQ=255)
                                                      ccGTGTTTCTTTCCCCCCAACACCCGCGCTTTTACCCACCCCCATTTGAAAAATTCCCTCCGCGGCGGCTGTCTCCCAGTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCg                                                                                                 <  1:262317/151‑1 (MQ=255)
                                                      ccGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCGGAGaaaaaaattttaacccc                                                                                                  >  4:268662/1‑137 (MQ=25)
                                                       ccgtaTTCCTTTCCCGCCACCAACCGCCATTTTACCCACCGCCATGTCAAAAAATGCCTCCGCGGGGGCTGTCTGCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGccc                                                                                                  <  4:313013/146‑1 (MQ=255)
                                                          taattcttcccccccAACACCCGCGCTTTTACCCACCCCCTTGTAAAAAAAGCCCTCCGCGGGGGCTGCCTGCCAGTTTTTTTCCCGCGAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCt                                                                                                          <  4:723792/126‑1 (MQ=255)
                                                            ttCTTTCCCCCCAACACCCGCGCTTTTACCCACCCCCTTGTGAAAAAATGCTTCCGGGGCGGCTGTCTCCCAGTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtg                                                                                            <  1:324729/150‑1 (MQ=255)
                                                                 ttccccccAACACCCGCGCTTTTACCCACCCCCTTGTGAAAAAATCCCTCCGGGGCGGTTGTCGCCCAGTTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgt                                                                                           <  1:663391/141‑1 (MQ=255)
                                                                   ccccccaccaCCCGCGCTTTCACCCAACCCAATTTGAAAAAATGCCGCCGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgttgt                                                                                        <  4:931769/144‑1 (MQ=255)
                                                                              ccgcccttttccccACCGCCATTTCAAAAAATCCTTCCGCGGGGGCTTTCTCCCATTTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctg                                                                          <  1:1440791/138‑1 (MQ=255)
                                                                               cgctcttttACCCAACGCAATGTGAAAAAGTCCCTTGGCGGCGGCGTTCGGCCATTTTTTTTCCCGCCCCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctgct                                                                        <  4:1030254/145‑1 (MQ=255)
                                                                                 cgcTTTTACCCACCCCCATGTAAAAAAATCCCTCCGGGGGGGCTTTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGctgct‑‑g                                                                     <  1:1295545/150‑1 (MQ=255)
                                                                                     tttATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGAAAAAGCATTTAACCCCCCCTGTTGTGTGGCAATCgc                                                                            >  1:399145/1‑141 (MQ=37)
                                                                                     tttACCCACCCCCATGTAAAAAATTCCCCCCGGGGGGGCTGTCTCCCATTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGt                                                                   <  4:146337/148‑1 (MQ=255)
                                                                                              cGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAACATTAAACCCCCCATCTTTTGTGGGAAACGCtggt‑‑gggtaaaaattg                                                          >  1:1154509/1‑138 (MQ=25)
                                                                                                   tgtgCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGGGAAAAAACATTTAACCCCTCCTTTTGTGTGGGGAACGCTGCT‑‑GGGGACAAAATGGGAAggg                                                   >  1:568071/1‑150 (MQ=255)
                                                                                                        aaaaaTGCCTTCGGGGCGGTTGTCCGCCACTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAg                                                <  1:462100/148‑1 (MQ=255)
                                                                                                        aaaaaTCCCTTGGCGGCGGCTGTCGCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGaa                                                 <  4:1336957/147‑1 (MQ=255)
                                                                                                         aaaaTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGAAATATATTTTACACCCCCTTGTTTTGTGGGCATCCCCGCTGGGGTAAAAATGGGAAAGGGagtggg                                             >  2:19597/1‑146 (MQ=255)
                                                                                                          aaaTGCCTCCGGGGGGGCTGTCGCCCATTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTgcgg                                           <  4:429547/151‑1 (MQ=255)
                                                                                                          aaaTCCCTTCGCGGCGGCTGTCTCCCAGTTTTTTTCCCCCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTgcgg                                           <  1:1350146/151‑1 (MQ=255)
                                                                                                           aaTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAAAAATTTTAACCCCTCCTGTTGTGTGGGAATCGCTGCT‑‑GGTGAAAAAGTGGAAAGGGGAGGgcggg                                          >  1:842602/1‑150 (MQ=255)
                                                                                                            aTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGGGAAAAAACATTAAACCCCTCCTGTTTTGTTGGGAACCGTGCT‑‑GGGTAAAATTTGGGAAGGGGAgggggggg                                         >  3:91003/1‑144 (MQ=255)
                                                                                                                ccTTGGGGGCGGCTTTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTGCGGCGa                                        <  1:888597/149‑1 (MQ=255)
                                                                                                                        ggcggcTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTgcggcg                                         <  2:93248/141‑1 (MQ=255)
                                                                                                                        ggcgcttttCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTGCGGCGaa                                       <  4:1252503/135‑1 (MQ=255)
                                                                                                                                tctcCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGa                            <  1:645096/146‑1 (MQ=255)
                                                                                                                                  tgcccttttttttcCCGCCCAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCa                              <  4:807373/133‑1 (MQ=255)
                                                                                                                                   cccattttttttcCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgcg                      <  3:103474/141‑1 (MQ=255)
                                                                                                                                    caagtttttttcCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGaa                           <  1:1193358/136‑1 (MQ=255)
                                                                                                                                         ttttttcCCCCCGAAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg               <  4:28885/148‑1 (MQ=255)
                                                                                                                                           tttccccgcagCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCgcgc                     <  1:104101/137‑1 (MQ=255)
                                                                                                                                              ttCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGAGCCAGAGAAAAAGATTTTAAAACCTCAACTTGTGTGGGAATCCCTGCT‑‑GGGAACAAAGTGGGAAGGGGAGGGCGGCGGAACAGCTCCCGAACCCCGCCCATTggggt           >  2:135547/1‑145 (MQ=255)
                                                                                                                                                ccgccgCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGAAAAAAATTTTAAACCCCCCTGTTGTGTGGGGAACGCTGCT‑‑GGGTAAAAAGTGGGAAGGGGAGGGGGGGGGAACAACTCCAGAAACACGCCAAATGAggttgc        >  1:1120970/1‑145 (MQ=255)
                                                                                                                                                   ccgcAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCa    <  1:40710/151‑1 (MQ=255)
                                                                                                                                                      caacaaATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAAt  <  4:519473/150‑1 (MQ=255)
                                                                                                                                                         |                                                                                                                                                      
CTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCT‑‑GGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAAT  >  NC_000913/357740‑358036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: