Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	45.5%	142.2 / 37.1	88	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (3/45);  new base G (40/0);  total (43/45)
Fisher's exact test for biased strand distribution p-value = 6.74e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354141‑354435                                                                                                                                                    |                                                                                                                                                     ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGa                                                                                                                                                    <  1:941412/150‑1 (MQ=255)        ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcg                                                                                                                                            <  2:1199422/151‑1 (MQ=255)        ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATgcg                                                                                                                                            >  2:913408/1‑151 (MQ=255)         cGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcgc                                                                                                                                           <  1:730601/151‑1 (MQ=255)          gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcgc                                                                                                                                           <  2:743971/150‑1 (MQ=255)           tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTg                                                                                                                                         >  1:377984/1‑151 (MQ=255)           tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGaag                                                                                                                                              >  1:1652069/1‑144 (MQ=255)             aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                       <  2:481951/151‑1 (MQ=255)                 tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCTGGGAggg                                                                                                                                   >  1:915034/1‑151 (MQ=255)                  gACGGTTAACACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAggg                                                                                                                                   <  1:756919/150‑1 (MQ=255)                        tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtg                                                                                                                             <  2:30339/150‑1 (MQ=255)                         aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc                                                                                                                           <  1:534573/151‑1 (MQ=255)                         aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtgg                                                                                                                            <  2:1612790/150‑1 (MQ=255)                              tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGGAAAAAGGGCCGGAAGGGGAgggggg                                                                                                                           >  1:428263/1‑145 (MQ=255)                               ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg                                                                                                                       <  2:640993/149‑1 (MQ=255)                               ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg                                                                                                                       <  2:1281422/149‑1 (MQ=255)                               ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg                                                                                                                       <  2:538757/149‑1 (MQ=255)                                tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtggc                                                                                                                     <  2:1166371/150‑1 (MQ=255)                                 aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGAAAAAAAGGCTGAGAGGGCAggggggggg                                                                                                                        >  2:1494842/1‑145 (MQ=255)                                    ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGGAAGGGGCGGGGGGGGGGGG‑Gttt                                                                                                                >  1:443212/1‑150 (MQ=255)                                     tcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGG‑GGTTg                                                                                                               >  2:952860/1‑150 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGt                                                                                                              <  2:631436/150‑1 (MQ=255)                                       gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCa                                                                                                            <  1:783698/151‑1 (MQ=255)                                          cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGC‑GGTTGTCAtt                                                                                                          >  2:616084/1‑150 (MQ=255)                                             aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTTGGTGGGGGGGAAGAGGAGCTGAAAGGGCAGGGGGGGGGGGG‑GTTTggcctttc                                                                                                        >  1:634072/1‑142 (MQ=255)                                             aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGTTGGGGGGAAAGAAGCGCTGAAAGGGGCGGGGGGGGGGGC‑GGTTGTGATTcc                                                                                                        >  2:1571344/1‑149 (MQ=255)                                               tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGG‑GGTTGTTCTTCCgga                                                                                                     >  1:726742/1‑148 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGaa                                                                                                     <  1:528583/149‑1 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGGAAGGGCCGGGGGGGGGGGG‑GGTTGTCATTcccga                                                                                                     >  1:154137/1‑146 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGGTGGGCTGGAAGGGCGGGGGGCGGGGGC‑GGTTGTCATTCCGgaa                                                                                                    >  2:478881/1‑150 (MQ=255)                                                  cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGCGCTGGAAGGGGAGGGGGGGGGGGG‑GGTTGTCATTTCGaaa                                                                                                    >  1:908980/1‑148 (MQ=255)                                                    aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAagagag                                                                                               <  2:589117/151‑1 (MQ=255)                                                    aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAagagag                                                                                               <  2:1340855/151‑1 (MQ=255)                                                      gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCg                                                                                             <  2:139570/151‑1 (MQ=255)                                                      gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCg                                                                                             <  2:1315643/151‑1 (MQ=255)                                                      gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGGAAGGGCAGGTGGCGGGGGG‑GGTTTTTATTCCGAAaga                                                                                                  >  2:1566376/1‑146 (MQ=255)                                                      gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGCGCTGAAAAGGGAGGGGGGGGGGGC‑GGTTGTCATTCCGAAAGAGAGCg                                                                                             >  2:257733/1‑151 (MQ=255)                                                       tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCCGGAAGGGGAGGGGGGGGGGGG‑GGTTGTGATTCCGGAAGAGAGCg                                                                                             >  2:154750/1‑150 (MQ=255)                                                         aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCTGCAAGGGCAGGTGGGGGGGGG‑GGTTGTTATTCCGGAagagag                                                                                               >  2:735764/1‑146 (MQ=255)                                                         aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGGAGGGGGCGGGGGG‑GGTTGTCATTTCGAAAGAGAGCg                                                                                             >  2:1269725/1‑148 (MQ=255)                                                             aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCt                                                                                      <  2:1232233/151‑1 (MQ=255)                                                             aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGTGCCGAAAGGGCAGGGGGGGGGGGC‑GGTTGTATTTCCGCAAGAGAGCGGCAGTCt                                                                                      >  2:1519439/1‑151 (MQ=255)                                                               gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTg                                                                                     <  1:777773/150‑1 (MQ=255)                                                                 tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGCCATTCCGAAAGAGAGCGACAGTCTGGa                                                                                   <  2:461605/150‑1 (MQ=255)                                                                   ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGa                                                                                <  2:788105/151‑1 (MQ=255)                                                                    cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                               <  2:904306/151‑1 (MQ=255)                                                                     gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATc                                                                              <  1:650024/151‑1 (MQ=255)                                                                         aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGGAAAGGGAGGGGGGGGGGGG‑GGTTGTCATTTCGGAAGAGAGGGAAAGTCTGGgagaaagtg                                                                           >  2:304896/1‑145 (MQ=255)                                                                            ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGGGGGGGG‑GGTTGTGATTCCGAAAGAGAGCGGCAGACTGGGAGATCGTGAt                                                                         >  1:1386094/1‑149 (MQ=255)                                                                                     cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGCAAGGGCAGGGGGGGGGGGG‑GGTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGAACGTGGTGTgg                                                                     >  1:123608/1‑144 (MQ=255)                                                                                       cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGc                                                            <  1:306321/151‑1 (MQ=255)                                                                                       cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGCTGAAAGGGCAGGGGGCGGGGGC‑GGTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGc                                                            >  2:656837/1‑151 (MQ=255)                                                                                           agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCCGAAAGGGCAGGGGGCGGGGGG‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGGTGCGCACTc                                                              >  2:862879/1‑145 (MQ=255)                                                                                            gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGGGGGGGC‑GGTTGTCATTCCGAAAGAGAGCGACAGGCTGGGAGATCGGGATGTGGGGCCCTCGCCag                                                         >  1:481913/1‑149 (MQ=255)                                                                                               tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                                     <  1:1600358/150‑1 (MQ=255)                                                                                               tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGTAAGGGCAGGTGGGGGGGGG‑GCTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCCCTCGCCag                                                         >  1:596330/1‑146 (MQ=255)                                                                                                 aaGAGAGTATCTGCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTTTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCCCTCGCGAGAGAAGGc                                                  <  1:1648242/151‑1 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                  <  1:1009860/151‑1 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                  <  1:748473/151‑1 (MQ=255)                                                                                                  agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                 >  2:1582958/1‑151 (MQ=255)                                                                                                  agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGC‑GGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                 >  2:204623/1‑151 (MQ=255)                                                                                                   gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGG‑GTTTGTCCTTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCg                                                 >  2:1503021/1‑150 (MQ=255)                                                                                                    agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAt                                               <  2:547793/151‑1 (MQ=255)                                                                                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                              <  2:979024/151‑1 (MQ=255)                                                                                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                              <  1:878253/151‑1 (MQ=255)                                                                                                       gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                            <  2:647822/151‑1 (MQ=255)                                                                                                       gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGG‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGGGGCACTCGCAAGAGAAGGGGGTTAt                                            >  2:965611/1‑151 (MQ=255)                                                                                                             ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCt                                      <  2:26856/151‑1 (MQ=255)                                                                                                             ccAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGTTGGGGGGGAAGATGGGCGGAAAGGGGAGGGGGCGGGGGG‑GGTTTTGATTCCCAAAGAGAGCGGCAGTCTGGGAGATCGGGGTGGGGGGCCCCCGCCagaggaagggaat                                              >  2:151837/1‑134 (MQ=255)                                                                                                              cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCCGAAAGGGGAGGTGGGGGGGGG‑GTTTGTCATTTCGGAAGAGAGCGGCAGGCTGGAAGATCGGGGGGTGGCGCACTCGCAAGAGAAGGCGAATATGGCGCtg                                     >  1:296243/1‑151 (MQ=255)                                                                                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAATGCGCTGGAAGGGGAGGGGGCGGGGGG‑GGTTGTTATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCCAGAGAAGGGGAATATGGGGGtgg                                    >  1:1536955/1‑151 (MQ=255)                                                                                                                 ccATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtg                                  <  1:38090/151‑1 (MQ=255)                                                                                                                 ccATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGACGCtggtg                                  <  2:676911/151‑1 (MQ=255)                                                                                                                  cATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGAAAGGGGAGGGGGCGGGGGG‑GGTTGTCATTTCGGAAGAGAGCGGCAAGCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAAGCGGTTATGGCGCtggggg                                 >  1:496431/1‑151 (MQ=255)                                                                                                                    tCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGCGGGGGG‑GGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGGGGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                                 >  1:169287/1‑149 (MQ=255)                                                                                                                      cGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGG‑GGTTGTCATTCCGAAAGAGAGGGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGGGATTATGGCGCtggggg                                 >  1:840682/1‑147 (MQ=255)                                                                                                                        ggCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGGAAGGGGAGGGGGCGGGGGCGGTTTGTC‑TTTCGGAAGAGAGCGGCAGGCTGGGAGATTGTGATGGGGGGCACTCGCCAGAGAAGGGGATTATGGCGGTGGGGGGCAGcc                           >  1:464549/1‑151 (MQ=255)                                                                                                                             tGCCGAAGTGGCGGGGGGTTGGGGGAAAAATGCGCTGAAAGGGGCGGGGGCGGGGGC‑GGTTGTCGTTCCGAAAGAGAGCGACAATCTGGAAGATCGTGATGTGGGGCACTCGCAAGAGAAGGGGGTTATGGGGCTGGTGGACCGCCAGAtt                      >  1:1016252/1‑151 (MQ=255)                                                                                                                              gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                      <  2:1673824/150‑1 (MQ=255)                                                                                                                              gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTg                     <  2:914432/151‑1 (MQ=255)                                                                                                                                 gAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCa                  <  1:942399/151‑1 (MQ=255)                                                                                                                                  aaGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCaa                 <  2:1560721/151‑1 (MQ=255)                                                                                                                                      ggCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt             <  1:1187255/151‑1 (MQ=255)                                                                                                                                          gtggcTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTCGgcc         >  1:660659/1‑151 (MQ=255)                                                                                                                                           tggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc         >  1:658024/1‑150 (MQ=255)                                                                                                                                             gtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgcc      <  2:1602225/151‑1 (MQ=255)                                                                                                                                                ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg    <  2:956035/150‑1 (MQ=255)                                                                                                                                                 gggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgc  >  1:952638/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354141‑354435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑