Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913535,9180AG41.6% 135.2 / 18.6 89E70G (GAG→GGG) hyihydroxypyruvate isomerase
Reads supporting (aligned to +/- strand):  ref base A (16/36);  new base G (37/0);  total (53/36)
Fisher's exact test for biased strand distribution p-value = 1.59e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCA  >  NC_000913/535768‑536065
                                                                                                                                                       |                                                                                                                                                   
gTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGa                                                                                                                                                     <  1:1693800/151‑1 (MQ=255)
gTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGa                                                                                                                                                     <  2:1256555/151‑1 (MQ=255)
gTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGa                                                                                                                                                     <  1:1505065/151‑1 (MQ=255)
   ttGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAgcg                                                                                                                                                  >  2:486167/1‑151 (MQ=255)
   ttGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAgc                                                                                                                                                   <  1:167866/150‑1 (MQ=255)
     gagaAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGcgcg                                                                                                                                                <  2:174654/151‑1 (MQ=255)
      agaAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGGGGGGGAGCGCgg                                                                                                                                               >  2:921473/1‑151 (MQ=255)
                gcAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGCCTGGGCGGTGGGGGGgcgccggtttggctg                                                                                                                                      >  2:1002227/1‑139 (MQ=255)
                  aGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGTCTGGGCGGCGGGGGGGGGGGGGATTGCCTg                                                                                                                                      >  2:187964/1‑148 (MQ=255)
                    tgtgGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGCGGCGGGGGGGGGCGGGGTTGGCTGTAtt                                                                                                                                  >  1:1248817/1‑150 (MQ=255)
                     gtgGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGGACGGGCGGGGGGGGAGCGCGGGATTTCCTGTCtt                                                                                                                                  >  1:1066488/1‑149 (MQ=255)
                     gtgGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTcc                                                                                                                                <  2:946963/151‑1 (MQ=255)
                     gtgGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGGGGGGGGGGGCGGTATTGTCTGTAtt                                                                                                                                  >  2:54284/1‑149 (MQ=255)
                        gTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGGGGCGGGGGGGCGCGGGATTGCCTGTATTCCTgg                                                                                                                             >  2:664988/1‑151 (MQ=255)
                        gTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTgg                                                                                                                             <  2:16485/151‑1 (MQ=255)
                           ttCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGcc                                                                                                                           <  2:216805/150‑1 (MQ=255)
                           ttCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGcc                                                                                                                           <  1:150804/150‑1 (MQ=255)
                                ggcgTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCGCAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTgaag                                                                                                                     <  1:964831/151‑1 (MQ=255)
                                 gcgTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGGGGGGGGGGAGGGCGGGATTGCCTGTATTCCTGGCCGTg                                                                                                                        >  1:271747/1‑147 (MQ=255)
                                  agTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGGGGGGGGGGGGCGGTATGTCTGTATTTCTTGGCGTgga                                                                                                                      >  1:279547/2‑146 (MQ=255)
                                       aaTTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGGGGGGGGGGGCGGGATTTCCTGTATTTCTGGCCGCGGAGAAGAGtt                                                                                                              >  2:1493519/1‑151 (MQ=255)
                                            aTGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCggg                                                                                                         <  1:188177/151‑1 (MQ=255)
                                                ttCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTTGGCGGCGGGGGGGGGCGGGATTTCCTTTATTCCTGGCCGTGGAGGAAAGTTTCGGGGTgg                                                                                                     >  2:564636/1‑151 (MQ=255)
                                                  ccTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGc                                                                                                    <  1:887727/150‑1 (MQ=255)
                                                  ccTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGc                                                                                                    <  2:781007/150‑1 (MQ=255)
                                                  ccTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCg                                                                                                   <  2:743466/151‑1 (MQ=255)
                                                  ccTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTAGGCGGCGGGGGGGAGCGGTTTTGCCCGTATTTCTGGGCCGGGAGAAAAGTTTTGGGGTgg                                                                                                     >  2:845161/1‑149 (MQ=255)
                                                     tATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGt                                                                                                  <  2:1439382/149‑1 (MQ=255)
                                                      aTGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTagc                                                                                               <  1:6068/151‑1 (MQ=255)
                                                       tGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGATGGGGGGCGGGGGGGCGGGGGATTTCCTGTTTTTCTGGGCGCGAAGAAGAGGTTTggggggggggag                                                                                                >  1:608740/1‑140 (MQ=255)
                                                         aCTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGGGGGGGGGGGCGGGGTTGCCTGTATTTCTGGGCGTGGAGAAGAGGTTTGGGGTGGCGGagcag                                                                                             >  2:981180/1‑150 (MQ=255)
                                                          cTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGCGGGGGGGCGCGGGATTGCCTGTATTTCTGGCCGTGGAGAAGAGTTTTGGGGTgggggag                                                                                                >  1:465124/1‑141 (MQ=255)
                                                          cTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGAGCGGGGGATTTCCTGTATTTCTTGCCGGGGAGAAAAGGTTTGGGGTgggggggga                                                                                              >  1:141283/1‑141 (MQ=255)
                                                           tACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGGGGCGGGGGGGCGGGGGATTGCCTGTATTCCTGGCCGGGGAGGAGAGGTTTGGGGTgggggag                                                                                                >  2:457904/1‑140 (MQ=255)
                                                           tACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGGGCGCGGGATTGCCTGTATTCCTGGCCGTGAAGAAGAGGTTTGGGGTGGGGTagcagc                                                                                            >  2:24392/1‑149 (MQ=255)
                                                           tACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACAGGGGGGCGGGGGGGAGCGGGAGTGGCTGTATTCCTGGGCGTGGAGGAGGGGTTTGGGGTGGGGTaggag                                                                                             >  1:469846/1‑148 (MQ=255)
                                                            aCGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTagcagcag                                                                                          <  1:98833/150‑1 (MQ=255)
                                                             cGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGAGTGGGCGGGGGGGGGGGGCGGGATTGCCTGTATTTCTTGCCGTGGAGAAGAGGTTTGGGGTgg                                                                                                     >  1:1614587/1‑138 (MQ=255)
                                                                  tggaagaaTTAAAAAATGTGCTGGGGAGTAAAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGa                                                                                       <  2:249383/144‑1 (MQ=255)
                                                                   tGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGGGGCGGGGGGGCGCGGGATTGCCTGTATTTCTGGCCGTGAAGAAGAGGTTTGGGGTGGCGGAGCAGCAGCGAtt                                                                                     >  2:1689103/1‑148 (MQ=255)
                                                                    gaagaaTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGtt                                                                                 <  1:1081105/151‑1 (MQ=255)
                                                                      agaaTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGCGGGGGGGGGCGGGATTGCCTGTATTTCTGGCCGCGAAGAAGAGTTTCGGGGTGGGGGAGCAGCAGCGATTCGTTAt                                                                               >  1:1483868/1‑151 (MQ=255)
                                                                       gaaTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATg                                                                              <  1:797664/151‑1 (MQ=255)
                                                                       gaaTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGAAGGGGGGGGGGGGGGGGGCGGGATTGCCTGTATTTCTGGGCGGGAAGAAGAGGTTTGGGGGGGCGGAGCAGCAGCgggtagtt                                                                                 >  1:742953/1‑141 (MQ=255)
                                                                           tAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTTGGGGGCGGGGGGGGGCGGGATTGCCTGTATTTCTGGCCGGGGAGAAGGGTTTCCGGGTGGGGTTGGAGGAGAGGTTTGTTATTccc                                                                           >  1:1330830/1‑150 (MQ=255)
                                                                           tAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGGGGCGGGGGAGCGCGGGTTTGCCTGTATTACTGGGCGTGGAGAAGGGATTTGGGATGGCGGAGGAACCGCGAttaggta                                                                                >  2:554049/1‑140 (MQ=255)
                                                                             aaaCATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGGGCGCGGTATTGCCTGTATTCCTGGCCGGGGAGAAGAGTTTCGGGGTGGGGTAGCAGCAGCGATTCGTTATGccc                                                                           >  2:620304/1‑148 (MQ=255)
                                                                                cATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGCATGGGGGGCGGGGGGGGGCGGGATTGTCTGTATTTCTTGGCGGGGAGAAGAGGTTCGGGGGGGGGTAGGAGGAACGAGTCGtttttgccgggcg                                                                      >  1:1580145/1‑139 (MQ=255)
                                                                                  tgtgCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGCGGCGGGGGGGCGCGGGATTTCCTGTATTTCTGGGCGTGGAGAAGAGGTTTGGGGTGGGGGAGCAGCAGCGGTTTGTTTTGTCcgcgcg                                                                      >  1:533637/1‑148 (MQ=255)
                                                                                  tgtgCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGGGCGCGGGAATGCCTGTATTCCTTGCCGGGAAGAAGAGGTTCGGGGTGGCGGAGCAGCAGCGATTCGTTATGCCCGTGCGCt                                                                    >  2:431647/1‑150 (MQ=255)
                                                                                   gtgCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGGGGGGGATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGGGGGCGGAGCAGCAGCGAGTCGTTATGCCCGGGCGCTgg                                                                  >  2:39813/1‑151 (MQ=255)
                                                                                    tgCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGGGGGGGGGCGCGGGAATGGCTGTATTCCTGGCCGGGAAGAAGAGATTCGGGGTGGCGGAGCAGCAGCGATTCGTTATGCCCGTGCGCTggg                                                                 >  2:301501/1‑151 (MQ=255)
                                                                                    tgCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGGGGGCGGGATTGGCCGTATTCCTGGCCGTGAAGAAGGGTTTTGGGGTGGGGTAGCAGCAACGGTTCGTTATGCCCCTGCGCTggg                                                                 >  2:659221/1‑151 (MQ=255)
                                                                                     gCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGGGCGCGGGATTGCCTGTATTCCTTGCCGTGAAGAAGAGGTTCGGGATGGCGGAGCAGCAGCGATTTGTTTTTCCCGGGCGCTggg                                                                 >  2:1115488/1‑150 (MQ=255)
                                                                                         gCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGGGGGCGGGATTTCCTGTATTCCTGGCCGGGGAGAAGAGGTTCGGGGTGGCGGAGCAGCAGCGATTCGTTTTTCCCGGGCGCCGGGgaa                                                              >  2:379070/1‑149 (MQ=255)
                                                                                          cGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGAGCGCGGGATTGCCTTTATTTCTGGCCGTGGAGAAGAGGTTCGGGGTGGCGGAGCAGCAGCGATTCGTTATTCCCGTGCGCTGGGgaata                                                            >  1:1282616/1‑150 (MQ=255)
                                                                                           gAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGGGCGCGGGATTGCCTGTATTTCTGGCCGGGAAGAAGAGGTTTGGGGTGGCGGAGCAGCAGCGATTCGTTATGCCCGTGGGCTGGGgaataa                                                           >  1:1549075/1‑150 (MQ=255)
                                                                                             gTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGAGGGCGGGATTGCCTGTATTCCTGGCCGTGAAGAAGAGGTTCGGGGTGGCGTAGCAGCAGCGATTCGTTATGCCCGCGCGCTGGGTAATaaaa                                                         >  2:1021701/1‑150 (MQ=255)
                                                                                               aataaACTCGAACATACGCTGCACAATTTACCGGCGGGTGACAGGGGGGCGGGGGGGGGCGGGATTGCCTTTTTTCCCTGCCGGGAAGAAGAGTTTCGGGGGGGGGTAGCGGCAGGGATTCGTTATGCCCGGGGGCTGGGGAATaaaaaa                                                       >  1:503349/1‑150 (MQ=255)
                                                                                                 taaACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGAGGGCGGGATTGCCTGTATTCCTGGCCGTGAAGAAGAGGTTTGGGGTGGGGGAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGGAATaaaaaaa                                                      >  1:902411/1‑149 (MQ=255)
                                                                                                 taaACTCGAACATACGCTGCACAATTTACCGGCGGGCGACTGGGCGGCGGGGGGGGGCGGGATTGCCTGTATTCCTGGCCGGGGAGAAGAGGTTTGGGGGGGGGGAGCAGCAGCGATTTGTTATGGCCGGGCGGTGGGGAATaaaaaaaat                                                    >  1:872767/1‑149 (MQ=255)
                                                                                                  aaaCTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTa                                                   <  1:1479844/151‑1 (MQ=255)
                                                                                                   aaCTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTaa                                                  <  2:346335/151‑1 (MQ=255)
                                                                                                   aaCTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGCGGCGGGGGGGCGCGGGATTTCCTGTATTCCTGGCCGTGGAGAAAAGGTTCGGGGTGGCGGAGCAGCAGCGAGTTGTTATTGCCGTGCGCTGGGGAATaaaaaaa                                                      >  1:657865/1‑147 (MQ=255)
                                                                                                     cTCGAACATACGCTGCACAATTTACCGGCGGGAGAATGGGCGGGGGGGGGGCGCGGGATTGCCTGTATTTCTGGCCGTGAAGAAGAGGTTCGGGGTGGGGTAGCAGCAGCGAATCGTTATGCCCCTGCGCTGGGGAATAAAAAAATTAACt                                                >  2:628192/1‑151 (MQ=255)
                                                                                                       cGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACt                                                <  1:1699188/149‑1 (MQ=255)
                                                                                                          aCATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGGGGGCGGGGTTTACTTTTTTTCTGGGCGGGGAGAAGAGGTTTGGGGGGGGGGGGCAGCAGCGGTTCGTTATTCCCGCGGGCTGGGGAATaaaaaaa                                                      >  1:389446/1‑140 (MQ=255)
                                                                                                          aCATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTg                                           <  2:191539/151‑1 (MQ=255)
                                                                                                           cATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTgg                                          <  1:1291940/151‑1 (MQ=255)
                                                                                                              aCGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTc                                        <  1:26427/150‑1 (MQ=255)
                                                                                                              aCGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCg                                       <  1:1137848/151‑1 (MQ=255)
                                                                                                              aCGCTGCACAATTTACCGGCGGGGGACAGGGCGGGGGGGGGGGGCGGGGTGGGCTGTATTTCTGGCCCGGAAGAAGAGGGTGCGGGTGGGGGAGCAGGACCGAATCGTTATTGCCGCGCCCGTGGGATTAAAAAAAATAACTGTCttggcg                                       >  1:1036924/1‑146 (MQ=255)
                                                                                                               cGCTGCACAATTTACCGGCGGGTGACTGTGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAATAGTTTCTGAATAGCGTAGCAGCAGCGATTCGTTATGCCCCTTCGCTCGGTAATAAAAAAATTAACTCTCTGATct                                       <  1:116730/150‑2 (MQ=255)
                                                                                                               cGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCgg                                      <  1:1527360/151‑1 (MQ=255)
                                                                                                               cGCTGCACAATTTACCGGCGGGGGACCTGGCGGGGGGGGGGGGCGGGATTGCCTGGATTTCTGGCCGTGGAGGAGAGTTTCTGGGGGGGGGAGCAGCAACGAATCGGTTTGCCCGGGCGGGGGGGAAAAAAAAAAATAACTATCTGGGCgg                                      >  2:613552/1‑151 (MQ=255)
                                                                                                                 cTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGGGCGCGGGATTGCCTGTATTCCTGGGCGGGGAGAAGAGGTTTTGGGGGGGGGAGCAGCAGCGGTTCGTTATGCCCCGGCGCTGGGGAATAAAAAAAATAAATGTCTGGTCgg                                      >  1:1301065/1‑149 (MQ=255)
                                                                                                                   gCAGAATTTCCCGGCGGCTGAGTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGGCTGGTCGGTaat                                  <  1:1034551/151‑2 (MQ=255)
                                                                                                                        aTTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGcc                             <  2:1334739/151‑1 (MQ=255)
                                                                                                                          ttACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCg                            <  2:596647/150‑1 (MQ=255)
                                                                                                                            aCCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCt                         <  2:1611358/151‑1 (MQ=255)
                                                                                                                             ccGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTg                        <  1:486151/151‑1 (MQ=255)
                                                                                                                                  gggTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTc                   <  1:1015159/151‑1 (MQ=255)
                                                                                                                                   ggTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTca                  <  1:1271360/151‑1 (MQ=255)
                                                                                                                                      gACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTcagc                >  2:1004289/1‑150 (MQ=255)
                                                                                                                                      gACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTcagc                >  1:1369527/1‑150 (MQ=255)
                                                                                                                                       aCTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTcagcag              <  2:769353/151‑1 (MQ=255)
                                                                                                                                            ggcggcGGGGGAGCGCGGTATTGCCTGGATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGACGGTAAAACGCCGGCTGGTTTCAGCAGTGa           >  1:6416/1‑150 (MQ=255)
                                                                                                                                              cggcggGGGAGAGCGGTAGTGCCTGTATTCCTAGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTTGTTATGCCCGGGCGCTGGGGTATACAAAAATTAACTGTCCGGTCGGTAAAAAGCCGGCTGGTTTCAGCCGCGaa          >  1:1297770/1‑149 (MQ=255)
                                                                                                                                               ggcggGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAg       <  1:457888/151‑1 (MQ=255)
                                                                                                                                                 cggGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAAc         >  1:1524934/1‑147 (MQ=255)
                                                                                                                                                  gggggAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGAtt    >  2:1034818/1‑151 (MQ=255)
                                                                                                                                                    gggAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCa  <  2:1185763/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
GTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCA  >  NC_000913/535768‑536065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: