Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,245,2340GT45.5% 119.4 / 62.7 99L25F (TTG→TTTycgYuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (14/40);  new base T (45/0);  total (59/40)
Fisher's exact test for biased strand distribution p-value = 4.01e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATT  >  NC_000913/1245086‑1245384
                                                                                                                                                     |                                                                                                                                                        
tCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTg                                                                                                                                                          <  1:671671/149‑1 (MQ=255)
       ccGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCt                                                                                                                                                 <  1:1434541/150‑1 (MQ=255)
        cGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTa                                                                                                                                               <  1:1227983/151‑1 (MQ=255)
          aTAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTtttt                                                                                                                                             >  1:1066605/1‑151 (MQ=255)
            aaaGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGa                                                                                                                                           <  2:523955/151‑1 (MQ=255)
              aGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTgcgcctttt                                                                                                                                             >  2:922581/1‑142 (MQ=255)
               gTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACa                                                                                                                                        <  1:1275705/151‑1 (MQ=255)
                 cGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACAt                                                                                                                                       <  1:263321/150‑1 (MQ=255)
                     tCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAACAttt                                                                                                                                     >  1:186785/1‑148 (MQ=255)
                      cccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGCGGGGTTATTGAAAAttt                                                                                                                                     >  1:1419010/1‑147 (MQ=255)
                      cccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGGGCTTTTTTAAAAATTAGa                                                                                                                                  >  1:3375/1‑150 (MQ=255)
                       ccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAAAAttt                                                                                                                                     >  1:427457/1‑146 (MQ=255)
                          gTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGGGCTTATTTAACAATTTGAAtt                                                                                                                               >  2:1551497/1‑149 (MQ=255)
                             acaacaacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGGTTTTTTAAAATTTTGAAtt                                                                                                                               >  1:1233510/1‑146 (MQ=255)
                                   acaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCtt                                                                                                                    <  1:242923/151‑1 (MQ=255)
                                     aGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGGCCCTTTTGTACATTTAGAATTGa                                                                                                                             >  2:1384952/1‑140 (MQ=255)
                                     aGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTTAAAAATTTGAAATGATTAATCCTTCt                                                                                                                  >  1:1377723/1‑151 (MQ=255)
                                          ccTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTTAAAATTTTGAAATTATCAATCCTTCt                                                                                                                  >  1:1441060/1‑146 (MQ=255)
                                          ccTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCa                                                                                                              <  2:1136800/150‑1 (MQ=255)
                                          ccTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACAATTAGAATTGATCAATCCTTCTgg                                                                                                                >  2:379245/1‑148 (MQ=255)
                                            ttttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAACATTTATAATTTATCAATCCtt                                                                                                                    >  2:1533650/1‑142 (MQ=255)
                                             tttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTTGCGTTTTTTAACATTTTGAATTTATTAATCCtttttgggga                                                                                                            >  1:1130655/1‑141 (MQ=255)
                                             tttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGAt                                                                                                           <  2:634838/150‑1 (MQ=255)
                                               tttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAACATTTTAAAATTATTAATCCTTTTTGCAGAtt                                                                                                          >  2:1682225/1‑149 (MQ=255)
                                               tttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTTGCAGAtt                                                                                                          >  2:1277288/1‑149 (MQ=255)
                                               tttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCCGCCCTTATTTAACATTTAAAAATGATTAATCCTTCTTGCAggat                                                                                                          >  1:103450/1‑146 (MQ=255)
                                                ttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTTTTGAAAATTTTGAATTTATCAATCCTTCTTGCAGATTaa                                                                                                        >  1:793220/1‑150 (MQ=255)
                                                ttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTGAACATTTTGAAATGTTCAATTCTTTTGGCCGGTTaa                                                                                                        >  1:631490/1‑150 (MQ=255)
                                                ttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCCTTTTTAAAATTTTGAAATTATCAATCCTTTTTGCAGAtt                                                                                                          >  1:212119/1‑148 (MQ=255)
                                                 tAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAg                                                                                                       <  1:798198/150‑1 (MQ=255)
                                                  aaTTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAACATTTAGAATTTATCAATCCTTCTGGCAGATTAAGcc                                                                                                     >  2:658825/1‑151 (MQ=255)
                                                   attattGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCa                                                                                                    <  1:946378/151‑1 (MQ=255)
                                                        tGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTTAACATTTTGAATTGATCAATCCTTCTGGGCGATTAAACCaaaa                                                                                                 >  2:1613313/1‑149 (MQ=255)
                                                        tGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAAt                                                                                                <  2:104870/150‑1 (MQ=255)
                                                          tttCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAAAATTTAGAATTGATTAATCCTTCTGGGAGATTAAGACaaaaaaac                                                                                             >  1:1579800/1‑147 (MQ=255)
                                                          tttCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTTGCTGCGCTTTTTTAAAAATTTGAATTTATTAATCCTTCTTGCAGATTAAGCCaaaaaaac                                                                                             >  1:111334/1‑147 (MQ=255)
                                                          tttCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGATTTTGGTGCGCTTATTGTACATTTAAAATTGATCAAACCTTCTGGGAAAATTAACCAAAATaac                                                                                             >  2:1264277/1‑149 (MQ=255)
                                                             cGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCCTTTTGAAAAATTATAATTGATCAAACCTTCTGGCCGATTAAAACCAAAAAccc                                                                                            >  1:1097161/1‑149 (MQ=255)
                                                             cGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGt                                                                                          <  2:584805/151‑1 (MQ=255)
                                                              gttgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAACATTTAGAAATGATTAATCCTTCTGGGAGATTAAGGCaaaaaaaccctt                                                                                         >  1:585814/1‑143 (MQ=255)
                                                              gttgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTTAACATTTATAATTGATCAATTCTTCTTGCCGATTAAGCCAAAATAacc                                                                                            >  1:663380/1‑148 (MQ=255)
                                                                tgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTc                                                                                        <  1:290887/150‑1 (MQ=255)
                                                                 gttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCgg                                                                                      <  2:10550/151‑1 (MQ=255)
                                                                   ttttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCCtt                                                                                         >  2:1493813/1‑146 (MQ=255)
                                                                   ttttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTTAAAATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAAAAACCGTTCgg                                                                                      >  1:1424491/1‑149 (MQ=255)
                                                                   ttttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGc                                                                                     <  2:265556/150‑1 (MQ=255)
                                                                      tGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAAt                                                                                  <  1:1704423/150‑1 (MQ=255)
                                                                          gtgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTc                                                                             <  1:70371/151‑1 (MQ=255)
                                                                          gtgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTc                                                                             <  2:13310/151‑1 (MQ=255)
                                                                             aTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAACATTTAGAATTGATCAATACTTCTGGCAGATTAAGCCCAAATACCCGTTTGGCAATGCTTTAg                                                                           >  1:477394/1‑150 (MQ=255)
                                                                               tCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTTAACATTTTTAATTGATCAATCCCTCTGGGAGAATAAACCAAAATAACCGTTCGGCAATGCTTCTGtt                                                                         >  2:1377487/1‑150 (MQ=255)
                                                                                  gAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGGTGCGCTTTTTTAACATTTAGAATTTTTCAATCCTTCTGGCAGAATAAACCAAAATAACCGTTCGGCAATGCTTCAGTTAc                                                                       >  2:277389/1‑149 (MQ=255)
                                                                                  gAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTAcgc                                                                     <  1:793891/151‑1 (MQ=255)
                                                                                  gAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTAcgc                                                                     <  2:1212729/151‑1 (MQ=255)
                                                                                   aaaaTAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTTAACAATTAGAATTTATCAAACCTTTTGGCAGATTAAGCCAAAAAAACCGTTCGGCAATGCTTCAGTTAcgc                                                                     >  2:744559/1‑150 (MQ=255)
                                                                                   aaaaTAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTAcgc                                                                     <  2:828422/150‑1 (MQ=255)
                                                                                     aataaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTTAAAATTTAGAAATGATCAATCCTTCTTGCAGATTAAGCCAAAAAAACCGTTCGGCAATGCTTTAGTTAcgcg                                                                   >  2:422642/1‑149 (MQ=255)
                                                                                     aataaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTTGGGGCGCTTATTTAAAAATTAGAAATTATCAAACCTTCTGGCAGATTAAGCCAAAATAACCGTTCGGCAATGCTTTAGGTAcgcg                                                                   >  1:309270/1‑149 (MQ=255)
                                                                                       taaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTAagc                                                                     >  2:482136/1‑146 (MQ=255)
                                                                                       taaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGa                                                                  >  2:284020/1‑148 (MQ=255)
                                                                                        aaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAaga                                                               <  2:1096387/150‑1 (MQ=255)
                                                                                            atTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCCTTTTTAAAAATTTAAATTTATCAATCCTTTTGGGAGAATTAACCAAAAAACCCGTTCTGGAATTGTTCCGTTTAGCGGAGAggga                                                           >  1:347954/1‑148 (MQ=255)
                                                                                             ttttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTGAAAATTTTGAATTTATCAATCCTTCTTGCAGATTAAGCCAAAAAAACCGTTCGGCAATGCTTCAGTTACGCGAAGAggga                                                           >  2:621866/1‑147 (MQ=255)
                                                                                              tttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGTACATTTAGAAATTATCAATCCCTCTTGCAGATTTAACCAAAATACCCCTTCCGGAAAGCTTCAGTTTCGCGGAgggg                                                             >  1:735903/1‑146 (MQ=255)
                                                                                              tttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGGTGCGCTTATTTAAAATTTAAAATTGATAAAAACCTCTGGCAGATTAAAGCAAAAAAACCGGTCGGCAAATCTTCCGTTACGCGGAGAGGGATgg                                                        >  1:1684048/1‑151 (MQ=255)
                                                                                               ttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATgg                                                        <  2:237948/150‑1 (MQ=255)
                                                                                               ttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATgg                                                        <  2:107893/150‑1 (MQ=255)
                                                                                                 aGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGGGAGGCGGATTTTGCCGCGCTTCTTTAAAATTTTGAAATGATCAATCCCTTTGGGAGATTAAACCCAAATATCCGCTGCGCAATGCCTTAGTTACGCGGAGAGGGAAGGtt                                                      >  2:1402452/1‑149 (MQ=255)
                                                                                                  gACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTTTTGCAGATTTTAAAATGATTAATCCCTTTGGCAGATTAAGCCAAAATACCCGCTCCGCAATGCTTCAGCTAAGCGGAGAgg                                                             >  2:709792/1‑142 (MQ=255)
                                                                                                   aCAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTTTTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATAACCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATgggc                                                      >  1:376431/1‑146 (MQ=255)
                                                                                                      gTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCCCTTTTTGAACAATTAGAATTGTTCAATCCCTCTTGGAGATTAAGCCAAAATAACCGTTCGGCAATTCTTCAGTTACGCGAAGGGGAATGGTCttgatt                                                >  1:751436/1‑146 (MQ=255)
                                                                                                      gTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCCGCGCTTTTTTAACTTTTTAAATTGATTAATCCTTCTGGGAGATTAAGGCCAAAAAACCGTTCGGCGATGCTTTCGTTTCGCGAAGAGGGATGGGCTGAAtt                                                >  1:1325282/1‑151 (MQ=255)
                                                                                                       tAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTTTTTAAAAATTTGAAATTATCAATACTTCTTGGAGGATTAGCCCAAATACCCCTTCGGCAATGCTTTTGTTAAGCGGAGAGGGATgggatggaatt                                               >  1:1167757/1‑142 (MQ=255)
                                                                                                          ccAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGGAGATTAAACCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGGATGGTCTGAATTCttt                                            >  2:284771/1‑151 (MQ=255)
                                                                                                          ccAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCtt                                             <  2:1137665/150‑1 (MQ=255)
                                                                                                           cAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCtttt                                           <  2:1377773/151‑1 (MQ=255)
                                                                                                            aGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAAAATTTAGAATTGATCAATCCTTCTGGCAGATTAAACCAAAATACCCGGTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCttttt                                          >  2:1398951/1‑151 (MQ=255)
                                                                                                            aGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCttttt                                          <  1:1269146/151‑1 (MQ=255)
                                                                                                              tCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTGAACATTTAGAATTTATCAAACCTTCTGGCAGATTAAGACAAAATAACCGTTTGGCAATGCTTCAGTTAAGCGGAGAGGGATGGGCTGAATTCttttttt                                        >  1:1543267/1‑151 (MQ=255)
                                                                                                                  gCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGtt                                    <  1:1276414/151‑1 (MQ=255)
                                                                                                                    gTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGc                                  <  2:1579863/151‑1 (MQ=255)
                                                                                                                      tAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTTTTTAACATTTAAAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACACCTTCCGCAATGCTTTCGTTACGCGAAGAGGGATGGTCTGAATTCTTTTTTTTGTTGCt                                 >  2:1173704/1‑150 (MQ=255)
                                                                                                                       aaaaaTGGGGCGGATAGTGAGGCGGCTTTTGCTGCGCTTTTTTAAAATTTAGAATTTATCAATCCTTCTTGCAGAATAAACCAAAATAACCGTTCGGCAATGCTTCAGTTACGCGGAAGGGGAAGGTTTGAATTTTTTTTTTTGTTGCt                                 >  2:1045005/1‑149 (MQ=255)
                                                                                                                        aaaaTGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTaaa                              <  2:258712/151‑1 (MQ=255)
                                                                                                                         aaaTGGGGCGGATAGTGAGGCGGTTTTTGGGGCGGTTTTTTAAAATTTAGAATTGATCAAACCTTCTGGCCGATTAAGCCCAAAAACCCCTTCGGCAATGCTTCAGTTTCGCGGAGAGGAAGGGGGTTAATTTTTTTTTTTGGTGTTaaa                              >  1:1505267/1‑150 (MQ=255)
                                                                                                                            tGGGGCGGATAGTGAGGCGGTTTTTGGTGCGGTTATTGAACATTTTGAATTGATCAATCCCTCTTGCAGAATAAGACAAAATAACCGTTCGGCAATGGTTTAGGTACGCGGAGAGGGATGGGCTGAATTCTTTTTTTTGTTGGTAAAt                             >  2:172742/1‑148 (MQ=255)
                                                                                                                            tGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAACATTTTGAATTTATCAATCCTTCTGGCAGATTAAGCCAAAATACCCCTTCGGCAATGCTTCAGTTACGCGGAGAGGGATGGGCTGAATTCTTTTTTTTGTTGCTAAATtct                          >  2:846226/1‑151 (MQ=255)
                                                                                                                             ggggCGGATAGTGAGGCGGTTTTTGGTGCGCCTTTTTGACATTTTGAATTGATCAATCCTTCTGGGAGAGTAAGCCCAAAAACCCGTTCGGCAATGCTTTAGTTTCGCGCAGAGGGAAGGTGTGAATTTTTTTTTTTGGTGCTAAATtct                          >  1:1530636/1‑150 (MQ=255)
                                                                                                                                gCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTgg                      <  1:922547/151‑1 (MQ=255)
                                                                                                                                    aTAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAgtg                  <  2:1609710/151‑1 (MQ=255)
                                                                                                                                       gTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCt               <  2:1172308/151‑1 (MQ=255)
                                                                                                                                          aGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGa            <  2:793245/151‑1 (MQ=255)
                                                                                                                                             cGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTa         <  2:1419061/151‑1 (MQ=255)
                                                                                                                                              ggTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAt        <  2:1167801/151‑1 (MQ=255)
                                                                                                                                              ggTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAt        <  2:168362/151‑1 (MQ=255)
                                                                                                                                               gTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtg       >  1:1662056/1‑151 (MQ=255)
                                                                                                                                               gTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtg       <  2:924552/151‑1 (MQ=255)
                                                                                                                                                    tGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGat   >  1:1238995/1‑151 (MQ=255)
                                                                                                                                                    tGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGa    <  2:1477742/150‑1 (MQ=255)
                                                                                                                                                     ggCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGatt  >  2:644041/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
TCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATT  >  NC_000913/1245086‑1245384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: