Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,486	0	C	A	42.4%	131.8 / 54.2	85	intergenic (+291/‑165)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (41/8);  new base A (0/36);  total (41/44)
Fisher's exact test for biased strand distribution p-value = 1.43e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATT  >  NC_000913/29337‑29636                                                                                                                                                           |                                                                                                                                                       ttttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCcac                                                                                                                                                        <  2:50774/150‑1 (MQ=255)   ttgttgTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACaa                                                                                                                                                      >  2:1575454/1‑150 (MQ=255)        gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAaataat                                                                                                                                                >  2:970523/1‑151 (MQ=255)          tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                              >  2:1316080/1‑151 (MQ=255)          tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                              >  1:258515/1‑151 (MQ=255)            tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                             <  2:457510/150‑1 (MQ=255)              aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATaaaa                                                                                                                                          <  2:1515201/151‑1 (MQ=255)                  aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATc                                                                                                                                      <  1:1689512/151‑1 (MQ=255)                     ttttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATccc                                                                                                                                    <  1:558972/150‑1 (MQ=255)                      tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCg                                                                                                                                   <  1:761737/150‑1 (MQ=255)                         gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCa                                                                                                                                <  2:30329/150‑1 (MQ=255)                         gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                               >  2:1531691/1‑151 (MQ=255)                            cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                             >  1:1227044/1‑150 (MQ=255)                              tttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGt                                                                                                                          >  1:70164/1‑151 (MQ=255)                               ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAg                                                                                                                           >  2:929225/1‑149 (MQ=255)                                    ccACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtt                                                                                                                    >  2:1383652/1‑151 (MQ=255)                                       cTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATCTAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                  >  2:1410347/1‑150 (MQ=255)                                        ttttttCTGCTTTTTTTTATTTCATCCAT‑TCTTTTGGCTGCGCAAGCTTTTTCCAGAACAGTTT‑AAAT‑AATTTTTTTGTCGTTTAA‑GCCC‑TGAAAAACATGCAGGACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTa                                                                                                                 <  1:1636702/150‑1 (MQ=255)                                          ttttCTGCTCTTTTTTATTTCATGCAT‑TTTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTA‑AGAT‑GATTTTTTTGTCGTTTAA‑TCCT‑TGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAgc                                                                                                               <  2:207841/150‑1 (MQ=255)                                               tGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                         >  1:1453310/1‑151 (MQ=255)                                                    ttttttATTCCA‑GCAATTTTTTTTGCTGCG‑AAACCTTTTCCAAGAAAAGTT‑AAATGGATTTTTTTGTCACTAAC‑TGCG‑GAAAAAACTTGCATCGCCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                       <  1:1591177/147‑1 (MQ=255)                                                    gTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGCCTTTTAGCGCCCATAtctc                                                                                                    >  1:1235369/1‑151 (MQ=255)                                                    gTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                    >  1:743314/1‑151 (MQ=255)                                                    gTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                    >  1:123544/1‑151 (MQ=255)                                                    gTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                    >  2:126771/1‑151 (MQ=255)                                                     ttttttttTCATGCAT‑TTTTTTGGCTGCGCAAGC‑TTTTCCAAAAAAAGTTAAAAT‑AATTTTTTTGTCTTTTAA‑TCCC‑TAAAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATAtc                                                                                                      <  1:351636/148‑1 (MQ=255)                                                      ttttttttCATGAAT‑TCTTTTTGCTGCGAAGGCTTTTCCCAAAACAGGTA‑AGAT‑GTTTTTTTTGTCTTTAAA‑TCCT‑TAAAAAACAGGCATGACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                   <  2:667875/150‑1 (MQ=255)                                                       tttATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                   >  2:553215/1‑149 (MQ=255)                                                        ttATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                   >  1:365852/1‑148 (MQ=255)                                                        ttATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                   >  2:1136938/1‑148 (MQ=255)                                                        ttATTTCATGAAA‑TCTTCTTGCTGCGCAAGCTTTTTCCAGAACAGTTT‑AGAT‑GTTTTTTTTGTCGTTAAA‑TGCC‑TGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAg                                                                                                 <  2:1358684/150‑1 (MQ=255)                                                          aTTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                              >  2:1581053/1‑151 (MQ=255)                                                           tttCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                              >  2:372788/1‑150 (MQ=255)                                                           ttccATCCAA‑TTTTTTTGCGGCAGAGGCTTTTTCCAAAACGGGTT‑ATAT‑ATTTTTTTTGACTATAAC‑TCCA‑TGTAAAACATGAATGAGCCAAAAAAAATTAAAAAAAAACCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                <  1:384036/145‑1 (MQ=255)                                                            ttCAAGTAA‑TCTTTTTGCTGCCCAGCCTTTTCCCAGAACAAGAT‑ATAT‑TTTTTTTTTGTCG‑TTAA‑GCCCTTAAAAAACAGCAATGACCCAAAAAAAAAAAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                              <  1:1103200/149‑1 (MQ=255)                                                                 gaaa‑TCTTCTTGCTGCACAAGTTTTTCCAAAAACAGGTT‑AAAT‑TATTTTTTTTTCCTTTAA‑TGCC‑TGAAAAACATGCTTGACCCACAAAATAATATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                            <  1:1323798/144‑1 (MQ=255)                                                                    attcttctTGCGGCGAAAGCGTTTCCAAGAACA‑GTT‑AGGA‑TATTTTTTTTTTCCTTAATTGCC‑TGAAAAACTGGCTGGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                          <  2:1230710/144‑1 (MQ=255)                                                                      ttttcttGCTGCACAAGCTTTTCCAAAAACAGTTT‑AGAT‑TTTTTTTTTGTCGCTTAA‑TGCT‑TTTAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                    <  1:1356285/148‑1 (MQ=255)                                                                        ttcttGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                 >  1:73754/1‑151 (MQ=255)                                                                         tcttGCTGCGCAAGCTTTTCCCAGAACAGGTT‑AGAT‑GTTTTTTTTGTCGTTTAA‑TGCC‑TGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTg                                                                                   <  2:789992/148‑1 (MQ=255)                                                                          cttGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                 >  1:372946/1‑149 (MQ=255)                                                                            tgctgcGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAg                                                                               >  2:324478/1‑149 (MQ=255)                                                                            tgctgcGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                              >  1:883731/1‑150 (MQ=255)                                                                              ccgagcaactttttcCCAGAAAGGGTA‑AGAT‑AATTTTTTTGACCCTTAA‑CGCT‑TATAAAAGATGAAAAGCCCAAAAAATAAAAAAAAAACTTCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAg                                                                               <  2:983968/136‑1 (MQ=255)                                                                                    aaGCTTTTCCAAGAACAGTTT‑AGAT‑GTTCTTTTTGTCGTTTAA‑TGCC‑TAAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                      <  2:763181/150‑1 (MQ=255)                                                                                    aaGCTTTTCCAAGAACAGTTT‑AGAT‑ATTTTTTTTGTCTTTTAT‑CCCC‑TGAAAAACATGCAGGAGCAAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                       <  1:77589/149‑1 (MQ=255)                                                                                    aaGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                     >  2:1344561/1‑151 (MQ=255)                                                                                         tttccaaaaaCAGTTA‑AAAT‑AATTTTTTTTTCGTTTAA‑TCCT‑TTAAAAACATGCATGACCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                                 <  1:1383602/142‑1 (MQ=255)                                                                                          ttccaaGAACAGGTT‑AGAT‑GATTTTTTTGTCGCTAAA‑TGCC‑TGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAg                                                                <  2:1547235/145‑1 (MQ=255)                                                                                             caaGAACAGTTT‑AGAT‑GTTTTTTTTGTCCCTAAT‑TCCC‑TTAAAAACATGCAGGGCCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                            <  2:1223159/149‑1 (MQ=255)                                                                                               aaaaCAGGTT‑AGAT‑TTTTTTTTTGTCGTTAAA‑TGCT‑TAAAAAACAGGCATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                            <  1:907151/147‑1 (MQ=255)                                                                                                       tt‑AGAT‑TATTTTTTTTTCGCTTAA‑TCCC‑TAAAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                    <  1:184398/149‑1 (MQ=255)                                                                                                               gatCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                            >  1:173883/1‑151 (MQ=255)                                                                                                               gatCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                            >  1:1111028/1‑151 (MQ=255)                                                                                                                atCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                           >  2:902095/1‑151 (MQ=255)                                                                                                                  ttttttGTCGTTAAA‑TCCC‑TATAAAACATGCAGGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                          <  1:540496/149‑1 (MQ=255)                                                                                                                    ttttGTCGTTTAA‑TGCC‑TAAAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcat                                        <  1:1316034/150‑1 (MQ=255)                                                                                                                    ttttGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                       >  1:960026/1‑151 (MQ=255)                                                                                                                      ttGTCGTTTAA‑TGCC‑TAAAAAACATGCATGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatca                                      <  1:1435748/150‑1 (MQ=255)                                                                                                                      ttGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                     >  2:1218612/1‑151 (MQ=255)                                                                                                                          cgtttAT‑TGCT‑TGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                    <  1:689443/145‑1 (MQ=255)                                                                                                                          cGCTTAA‑TGCC‑TGAAAAACATGCAGGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                 <  2:650302/151‑1 (MQ=255)                                                                                                                           gCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                 >  1:1362100/1‑150 (MQ=255)                                                                                                                             ttAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                              >  2:1321159/1‑151 (MQ=255)                                                                                                                               aa‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                             >  1:278943/1‑150 (MQ=255)                                                                                                                                a‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                            >  2:1689709/1‑150 (MQ=255)                                                                                                                                 aatgcataaaaaaCAGGAATGACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                            <  1:1080292/142‑1 (MQ=255)                                                                                                                                 aatccgttaaaaaCATGTAGGACCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                            <  1:1121360/142‑1 (MQ=255)                                                                                                                                     c‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                       >  2:517600/1‑151 (MQ=255)                                                                                                                                      ctaaaaaaCTTGCATGACCCAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                             <  1:479970/141‑1 (MQ=255)                                                                                                                                         tAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                     >  1:990730/1‑150 (MQ=255)                                                                                                                                          aaaaCATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                       <  2:1515698/147‑1 (MQ=255)                                                                                                                                          aaaaCATGCATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                    <  1:657994/150‑1 (MQ=255)                                                                                                                                           aaaCATGAATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                  <  1:776037/151‑1 (MQ=255)                                                                                                                                            aaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                  >  2:370085/1‑150 (MQ=255)                                                                                                                                            aaCATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                 <  2:960516/151‑1 (MQ=255)                                                                                                                                              catgcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                >  2:224461/1‑150 (MQ=255)                                                                                                                                                tgcaggAGCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag              <  2:227783/150‑1 (MQ=255)                                                                                                                                                 gcatgAGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt             <  2:288212/150‑1 (MQ=255)                                                                                                                                                 gcatgACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt             <  2:614319/150‑1 (MQ=255)                                                                                                                                                  catgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg            >  1:1135932/1‑150 (MQ=255)                                                                                                                                                   atgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg            >  1:1356874/1‑149 (MQ=255)                                                                                                                                                      acccAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt         <  1:1531630/147‑1 (MQ=255)                                                                                                                                                      aaccAAAAAATAAAAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAgtgagtga       <  1:1206140/149‑1 (MQ=255)                                                                                                                                                      aGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg        >  2:1275383/1‑150 (MQ=255)                                                                                                                                                        ccAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat     <  2:261417/151‑1 (MQ=255)                                                                                                                                                          acaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat   >  1:1579368/1‑151 (MQ=255)                                                                                                                                                          acaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat   >  1:464855/1‑151 (MQ=255)                                                                                                                                                           caAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt  >  2:1250413/1‑151 (MQ=255)                                                                                                                                                           |                                                                                                                                                       TTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATT  >  NC_000913/29337‑29636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑