Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,496	0	T	A	46.8%	119.9 / 55.6	94	intergenic (+301/‑155)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (43/7);  new base A (0/44);  total (43/51)
Fisher's exact test for biased strand distribution p-value = 1.00e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29346‑29642                                                                                                                                                            |                                                                                                                                                   tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                    >  1:258515/1‑151 (MQ=255) tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                    >  2:1316080/1‑151 (MQ=255)   tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                                   <  2:457510/150‑1 (MQ=255)     aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATaaaa                                                                                                                                                <  2:1515201/151‑1 (MQ=255)         aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATc                                                                                                                                            <  1:1689512/151‑1 (MQ=255)            ttttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATccc                                                                                                                                          <  1:558972/150‑1 (MQ=255)             tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCg                                                                                                                                         <  1:761737/150‑1 (MQ=255)                gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCa                                                                                                                                      <  2:30329/150‑1 (MQ=255)                gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                                     >  2:1531691/1‑151 (MQ=255)                   cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                                   >  1:1227044/1‑150 (MQ=255)                     tttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGt                                                                                                                                >  1:70164/1‑151 (MQ=255)                      ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAg                                                                                                                                 >  2:929225/1‑149 (MQ=255)                           ccACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtt                                                                                                                          >  2:1383652/1‑151 (MQ=255)                              cTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATCTAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                        >  2:1410347/1‑150 (MQ=255)                               ttttttCTGCTTTTTTTTATTTCATCCAT‑TCTTTTGGCTGCGCAAGCTTTTTCCAGAACAGTTT‑AAAT‑AATTTTTTTGTCGTTTAA‑GCCC‑TGAAAAACATGCAGGACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTa                                                                                                                       <  1:1636702/150‑1 (MQ=255)                                 ttttCTGCTCTTTTTTATTTCATGCAT‑TTTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTA‑AGAT‑GATTTTTTTGTCGTTTAA‑TCCT‑TGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAgc                                                                                                                     <  2:207841/150‑1 (MQ=255)                                      tGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                               >  1:1453310/1‑151 (MQ=255)                                           ttttttATTCCA‑GCAATTTTTTTTGCTGCG‑AAACCTTTTCCAAGAAAAGTT‑AAATGGATTTTTTTGTCACTAAC‑TGCG‑GAAAAAACTTGCATCGCCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                             <  1:1591177/147‑1 (MQ=255)                                           gTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGCCTTTTAGCGCCCATAtctc                                                                                                          >  1:1235369/1‑151 (MQ=255)                                           gTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                          >  1:123544/1‑151 (MQ=255)                                           gTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                          >  2:126771/1‑151 (MQ=255)                                           gTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                          >  1:743314/1‑151 (MQ=255)                                            ttttttttTCATGCAT‑TTTTTTGGCTGCGCAAGC‑TTTTCCAAAAAAAGTTAAAAT‑AATTTTTTTGTCTTTTAA‑TCCC‑TAAAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATAtc                                                                                                            <  1:351636/148‑1 (MQ=255)                                             ttttttttCATGAAT‑TCTTTTTGCTGCGAAGGCTTTTCCCAAAACAGGTA‑AGAT‑GTTTTTTTTGTCTTTAAA‑TCCT‑TAAAAAACAGGCATGACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         <  2:667875/150‑1 (MQ=255)                                              tttATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         >  2:553215/1‑149 (MQ=255)                                               ttATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         >  2:1136938/1‑148 (MQ=255)                                               ttATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         >  1:365852/1‑148 (MQ=255)                                               ttATTTCATGAAA‑TCTTCTTGCTGCGCAAGCTTTTTCCAGAACAGTTT‑AGAT‑GTTTTTTTTGTCGTTAAA‑TGCC‑TGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAg                                                                                                       <  2:1358684/150‑1 (MQ=255)                                                 aTTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                    >  2:1581053/1‑151 (MQ=255)                                                  tttCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                    >  2:372788/1‑150 (MQ=255)                                                  ttccATCCAA‑TTTTTTTGCGGCAGAGGCTTTTTCCAAAACGGGTT‑ATAT‑ATTTTTTTTGACTATAAC‑TCCA‑TGTAAAACATGAATGAGCCAAAAAAAATTAAAAAAAAACCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                      <  1:384036/145‑1 (MQ=255)                                                   ttCAAGTAA‑TCTTTTTGCTGCCCAGCCTTTTCCCAGAACAAGAT‑ATAT‑TTTTTTTTTGTCG‑TTAA‑GCCCTTAAAAAACAGCAATGACCCAAAAAAAAAAAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                    <  1:1103200/149‑1 (MQ=255)                                                        gaaa‑TCTTCTTGCTGCACAAGTTTTTCCAAAAACAGGTT‑AAAT‑TATTTTTTTTTCCTTTAA‑TGCC‑TGAAAAACATGCTTGACCCACAAAATAATATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                                  <  1:1323798/144‑1 (MQ=255)                                                           attcttctTGCGGCGAAAGCGTTTCCAAGAACA‑GTT‑AGGA‑TATTTTTTTTTTCCTTAATTGCC‑TGAAAAACTGGCTGGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                                <  2:1230710/144‑1 (MQ=255)                                                             ttttcttGCTGCACAAGCTTTTCCAAAAACAGTTT‑AGAT‑TTTTTTTTTGTCGCTTAA‑TGCT‑TTTAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                          <  1:1356285/148‑1 (MQ=255)                                                               ttcttGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                       >  1:73754/1‑151 (MQ=255)                                                                tcttGCTGCGCAAGCTTTTCCCAGAACAGGTT‑AGAT‑GTTTTTTTTGTCGTTTAA‑TGCC‑TGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTg                                                                                         <  2:789992/148‑1 (MQ=255)                                                                 cttGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                       >  1:372946/1‑149 (MQ=255)                                                                   tgctgcGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAg                                                                                     >  2:324478/1‑149 (MQ=255)                                                                   tgctgcGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                                    >  1:883731/1‑150 (MQ=255)                                                                     ccgagcaactttttcCCAGAAAGGGTA‑AGAT‑AATTTTTTTGACCCTTAA‑CGCT‑TATAAAAGATGAAAAGCCCAAAAAATAAAAAAAAAACTTCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAg                                                                                     <  2:983968/136‑1 (MQ=255)                                                                           aaGCTTTTCCAAGAACAGTTT‑AGAT‑GTTCTTTTTGTCGTTTAA‑TGCC‑TAAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                            <  2:763181/150‑1 (MQ=255)                                                                           aaGCTTTTCCAAGAACAGTTT‑AGAT‑ATTTTTTTTGTCTTTTAT‑CCCC‑TGAAAAACATGCAGGAGCAAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                             <  1:77589/149‑1 (MQ=255)                                                                           aaGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                           >  2:1344561/1‑151 (MQ=255)                                                                                tttccaaaaaCAGTTA‑AAAT‑AATTTTTTTTTCGTTTAA‑TCCT‑TTAAAAACATGCATGACCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                                       <  1:1383602/142‑1 (MQ=255)                                                                                 ttccaaGAACAGGTT‑AGAT‑GATTTTTTTGTCGCTAAA‑TGCC‑TGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAg                                                                      <  2:1547235/145‑1 (MQ=255)                                                                                    caaGAACAGTTT‑AGAT‑GTTTTTTTTGTCCCTAAT‑TCCC‑TTAAAAACATGCAGGGCCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                                  <  2:1223159/149‑1 (MQ=255)                                                                                      aaaaCAGGTT‑AGAT‑TTTTTTTTTGTCGTTAAA‑TGCT‑TAAAAAACAGGCATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                                  <  1:907151/147‑1 (MQ=255)                                                                                              tt‑AGAT‑TATTTTTTTTTCGCTTAA‑TCCC‑TAAAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                          <  1:184398/149‑1 (MQ=255)                                                                                                      gatCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                                  >  1:1111028/1‑151 (MQ=255)                                                                                                      gatCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                                  >  1:173883/1‑151 (MQ=255)                                                                                                       atCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                 >  2:902095/1‑151 (MQ=255)                                                                                                         ttttttGTCGTTAAA‑TCCC‑TATAAAACATGCAGGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                <  1:540496/149‑1 (MQ=255)                                                                                                           ttttGTCGTTTAA‑TGCC‑TAAAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcat                                              <  1:1316034/150‑1 (MQ=255)                                                                                                           ttttGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                             >  1:960026/1‑151 (MQ=255)                                                                                                             ttGTCGTTTAA‑TGCC‑TAAAAAACATGCATGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatca                                            <  1:1435748/150‑1 (MQ=255)                                                                                                             ttGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                           >  2:1218612/1‑151 (MQ=255)                                                                                                                 cgtttAT‑TGCT‑TGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                          <  1:689443/145‑1 (MQ=255)                                                                                                                 cGCTTAA‑TGCC‑TGAAAAACATGCAGGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                       <  2:650302/151‑1 (MQ=255)                                                                                                                  gCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                       >  1:1362100/1‑150 (MQ=255)                                                                                                                    ttAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                    >  2:1321159/1‑151 (MQ=255)                                                                                                                      aa‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                   >  1:278943/1‑150 (MQ=255)                                                                                                                       a‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                  >  2:1689709/1‑150 (MQ=255)                                                                                                                        aatgcataaaaaaCAGGAATGACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                  <  1:1080292/142‑1 (MQ=255)                                                                                                                        aatccgttaaaaaCATGTAGGACCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                  <  1:1121360/142‑1 (MQ=255)                                                                                                                            c‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                             >  2:517600/1‑151 (MQ=255)                                                                                                                             ctaaaaaaCTTGCATGACCCAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                   <  1:479970/141‑1 (MQ=255)                                                                                                                                tAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                           >  1:990730/1‑150 (MQ=255)                                                                                                                                 aaaaCATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                             <  2:1515698/147‑1 (MQ=255)                                                                                                                                 aaaaCATGCATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                          <  1:657994/150‑1 (MQ=255)                                                                                                                                  aaaCATGAATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                        <  1:776037/151‑1 (MQ=255)                                                                                                                                   aaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                        >  2:370085/1‑150 (MQ=255)                                                                                                                                   aaCATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                       <  2:960516/151‑1 (MQ=255)                                                                                                                                     catgcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                      >  2:224461/1‑150 (MQ=255)                                                                                                                                      atggaagaacaaaaaaaaaaaATAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                      <  1:1554385/136‑1 (MQ=255)                                                                                                                                       tgcaggAGCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag                    <  2:227783/150‑1 (MQ=255)                                                                                                                                        gcatgAGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                   <  2:288212/150‑1 (MQ=255)                                                                                                                                        gcatgACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                   <  2:614319/150‑1 (MQ=255)                                                                                                                                         catgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                  >  1:1135932/1‑150 (MQ=255)                                                                                                                                          atgaccaaaaaaaTAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                  <  2:781770/140‑1 (MQ=255)                                                                                                                                          atgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                  >  1:1356874/1‑149 (MQ=255)                                                                                                                                             acccAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt               <  1:1531630/147‑1 (MQ=255)                                                                                                                                             aaccAAAAAATAAAAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAgtgagtga             <  1:1206140/149‑1 (MQ=255)                                                                                                                                             aGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg              >  2:1275383/1‑150 (MQ=255)                                                                                                                                               ccAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat           <  2:261417/151‑1 (MQ=255)                                                                                                                                                 acaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat         >  1:1579368/1‑151 (MQ=255)                                                                                                                                                 acaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat         >  1:464855/1‑151 (MQ=255)                                                                                                                                                 aaaaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                      <  2:1198670/136‑1 (MQ=255)                                                                                                                                                 aaaaaaTAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata          <  2:344259/148‑1 (MQ=255)                                                                                                                                                  caAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt        >  2:1250413/1‑151 (MQ=255)                                                                                                                                                   aaaaaaaaaaaaaaaaTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAgtgagtga             <  2:1008046/145‑1 (MQ=255)                                                                                                                                                   aaaaTAATAAAAAAACGCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc       <  1:1331127/151‑1 (MQ=255)                                                                                                                                                   aaaaTAAAAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat         <  1:901323/149‑1 (MQ=255)                                                                                                                                                    aaaTAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct      >  2:1585902/1‑151 (MQ=255)                                                                                                                                                     aataatAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtctc     <  1:1618045/151‑1 (MQ=255)                                                                                                                                                      ataataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct    >  1:585813/1‑151 (MQ=255)                                                                                                                                                        aataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg  >  2:659885/1‑151 (MQ=255)                                                                                                                                                        aataAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct    <  1:1061169/149‑1 (MQ=255)                                                                                                                                                         atatAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg  >  2:1539468/1‑150 (MQ=255)                                                                                                                                                            |                                                                                                                                                   TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAA‑TCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGAT‑GATCTTTTTGTCGCTTAA‑TGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29346‑29642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑