Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,676,106	0	C	A	49.4%	88.2 / 57.3	81	L240F (TTG→TTT)	yphD	putative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (36/5);  new base A (0/40);  total (36/45)
Fisher's exact test for biased strand distribution p-value = 6.45e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGAT  >  NC_000913/2675957‑2676252                                                                                                                                                      |                                                                                                                                                    aGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCcaac                                                                                                                                                     >  2:1335828/1‑150 (MQ=255)  gCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCcaacaa                                                                                                                                                   <  2:562772/151‑1 (MQ=255)    gTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACaaa                                                                                                                                                  >  1:353988/1‑150 (MQ=255)      aaTCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATg                                                                                                                                               >  1:440345/1‑151 (MQ=255)         cACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGcc                                                                                                                                             <  2:87233/150‑1 (MQ=255)            cAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑c                                                                                                                                        <  2:1470095/151‑1 (MQ=255)               cacaCCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CAcc                                                                                                                                     >  1:1285198/1‑151 (MQ=255)               cacaCCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CAcc                                                                                                                                     >  2:748352/1‑151 (MQ=255)                 cacCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CAccg                                                                                                                                    <  1:1040264/150‑1 (MQ=255)                  acCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CAccgcc                                                                                                                                  >  2:1629884/1‑151 (MQ=255)                         aaTGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAACTGCCGGT‑CACCGCCGCtaat                                                                                                                            >  1:325109/1‑150 (MQ=255)                          aTGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCtaata                                                                                                                           >  1:1184746/1‑150 (MQ=255)                               ccGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATccc                                                                                                                      >  1:1644514/1‑150 (MQ=255)                                  aaCGGGGACCCGCGACCGCCGGAAAGTGCCGTCCCGCCGACCACGCCCGCGGCGATGACGTCAAACCCCAGACCGTTTGCGGCACCGGCGTTCCCAGACCCGAGGCGCGCCGCCAAAAAAATGCCGGT‑CACCGCCGCTAATAATCCCGaa                                                                                                                   <  2:499088/150‑1 (MQ=255)                                         acccGCGCCCGCCGGAAAGTGCCGTCCCGCCGACCACGCCCGCGGCGATGACGTCAACCTCCAGCCCGTTTGCGGCACCTGCTTTCCCAGAACCGAGGCGCGCCGCAAAAAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTaa                                                                                                            <  2:59996/148‑1 (MQ=255)                                          cccggccccgcggGAAAGTGCCGCCCCGCCGCCCCCGCCCGCGGGGATAACGTCAAACCCCAGCCCGTTTGGGGCCCCGGCGTTACCAGAACCGGGGCGCGCCGCCAAAAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTaaa                                                                                                           <  1:313105/143‑1 (MQ=255)                                               gACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATa                                                                                                       >  1:374759/1‑149 (MQ=255)                                                aCCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATa                                                                                                       >  2:72076/1‑148 (MQ=255)                                                 ccgcggGAAAGTCCCGTCCCGCCACCCACGCCCGCGGCGAGGACGTCAACCTCCAGACCGTTGGCGGCCCCTGCGTTCCCAGACCCGAGGCGCGCCGCAAAAAAAATGCGGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATaa                                                                                                      <  2:7154/148‑1 (MQ=255)                                                 ccgcggGAAAGGCCCGTCCCGCCGCCCACGCCCGGGGCGATGACGCCAACCCCCAGCCCGTTTGCGGCACCTGCGTTCCCAGAACGGGGGCGCGCCGCAAAAAAAAGGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAg                                                                                                     <  1:705586/149‑1 (MQ=255)                                                  cgccgAAAAGGCCCGTCCCGCCGCCCACGCCCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTCCCAGACCCGAGCCCCGCCACCAAAAAAATGCGGGC‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAAt                                                                                                  <  2:574277/151‑1 (MQ=255)                                                   gcagaaaaggcgcGTCCCGCCGACCACGCCCGGGGCAAGAACGTAAACCCCCAGGCCGTTTGCGGCCCCGGCGTTACCAGACCCGGGGCGCGCCGCAAAAAAAAGGCCGTT‑CACCGCCGCTAATAATCCCGAAAGGGt                                                                                                              <  1:70471/126‑1 (MQ=255)                                                    cgggAAAGTCCCGTCCCGCCGACCCCGCCCGCGGCGAGAACGTAAAACCCCAGCCCGTTGGCGCCCCTTGCGTTACCAAACCCGGGGCGCGCGGCAAAAAAAAGGCCGGC‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAAt                                                                                                  <  1:923456/147‑1 (MQ=255)                                                    cgagAAAGGGCCGCACCCCCGCCCACGCCCGGGGCGATGACGTAAAACACCAAACCGTTGCCGCCCCCTGGGTTACAAAAACAGGGGGGGGCGGAAAAAAAAATGCGTGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAg                                                                                                     <  1:1239718/143‑1 (MQ=255)                                                    ccgGAAAGAGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgc                                                                                                >  2:21627/1‑151 (MQ=255)                                                      gaaaggTGCCGCCCCGCCGCCCCCGCCCGGGGCGATGACGTAAAACCCCAGACCGTTTGCGGCCCCTGCGTTACCAAAACCGAGGCGCGCCGCAAAAAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATaa                                                                                                      <  1:442672/138‑1 (MQ=255)                                                      gaaaaGTGCCGGCCCGCCGCCCACGCCCGCGGCGATGACGTAAACCTCCAGCCCGTTGGCGGCACCTGCGTTACCAAAACCGGGGCGCGCGGCAAAAAAAAGGCCGGT‑CGCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcg                                                                                               <  1:358966/148‑1 (MQ=255)                                                        aaaGGGCCTTCCCGCCGACCACGCCCGGGGCGATGACGTAAAACTCCAGCCCGTTGGCGCCCCCTGCGTTACCAGAACCGAGGCGCGCCGCAAAAAAAAGGCGGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAAt                                                                                                  <  1:1603501/145‑1 (MQ=255)                                                        aaaGGGCCGTCCCGCCGCCCGCGACCGCGGGGATGGCGTCAACCTCCGGCCCGTTTGCGGCCCCTGCTTTACCAGACCCGGGGCGCGCCGCAAAAAAAAGCCCGGT‑CGCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcg                                                                                               <  2:1380709/148‑1 (MQ=255)                                                         aaGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCAcc                                                                                           >  1:1188489/1‑151 (MQ=255)                                                           gTGCCGCCCCGCCGCCCCCGCCGGCGGCGAGGACGCCAACCCCCAGCCCGTTGGCGGCCCCTGCGTAACCAGACCCGAGGCGCGCCGCAAAAAAAAGGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCAccc                                                                                          <  1:772347/150‑1 (MQ=255)                                                              ccGTCCCGCCGCCCCCGCCCGCGGCGATGACGTCAAACCCCAGCCCGTTTGCGGCCCCTGCGTTCCCAGACCCGAGGCGCGCCCCCAAAAAAAGGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcga                                                                                        <  2:945081/149‑1 (MQ=255)                                                              ccGTCCCGCCGCCCACGACCGCGGCGATGACGTCAAACCCCAGACCGTTTGCGGCCCCTGCGTTACCAGACCCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCAccc                                                                                          <  2:1122347/147‑1 (MQ=255)                                                               cGTCCCCCCGACCCCGCCCGCGGCGATGACGTCAAACCCCAGACCGTTGGCGGCCCCGGCGTTCCCAAACCCGAGGCGCGCCGCAAAAAAAAGGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcgac                                                                                       <  1:1257306/149‑1 (MQ=255)                                                                  cccgccgCCCCCGCCCGCGGCGATGCCGTAAACCCCCAGCCCGTTTGCGCCCCCTGGGTCACCAGAACCGGGGCGCGCCGCAAAAAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACg                                                                                  <  1:14728/150‑1 (MQ=255)                                                                   ccgccgCCCACGCCCGCGGCGAGGCCGTCAACCCCCAGCCCGTTTGCGGCCCCTGCGTTCCCAGAACCGAGGCGCGCCGCAAAAAAAAGGCGGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGt                                                                                 <  1:733231/151‑1 (MQ=255)                                                                    cgccgcCCACGCCGGCGGCGATGCCGGCAATCTCCAGCCCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAAAAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAAc                                                                                   <  2:803949/148‑1 (MQ=255)                                                                          ccccgcccGCGGCGATGACGTCAAACTCCAGCCCGTTTGCGGCCCCTGCTTTCCCAGACCCGGGGCGCGCCGCCAAAAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGAt                                                                             <  2:760310/142‑1 (MQ=255)                                                                          ccccGACCGCGGCGATGACGTCAAACCCCAGCCCGTTTGCGGCCCCTGCTTTACCAGAACCGAGGCGCGCCGCAAAAAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATg                                                                            <  2:227645/146‑1 (MQ=255)                                                                          ccACGCCCGCGGCGATGACGTCAAACCCCAGCCCTTTTGCGGCCCCTGCGTTCCCAGACCCGAGGCGCGCCGCCAAAAAAATGCGGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGAt                                                                             <  1:1226481/148‑1 (MQ=255)                                                                          ccACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGcc                                                                          >  1:784515/1‑151 (MQ=255)                                                                          ccACGACCGCGGCGATGACGAAAAACCCCAGACCTTTTGCGCCCCCTGCGTTACCAAAACCGAGGGGCGCCGCAAAAAAAAGGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATg                                                                            <  1:1585473/149‑1 (MQ=255)                                                                              gACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGcac                                                                      >  2:248078/1‑151 (MQ=255)                                                                               cccGAGGCAATGACGTCAACCTCCAGCCGGTTTGCGACTCGTGAGTAACCAAACCCGAGGCGCGCGCCCAAAAAAATGCCGTT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGcac                                                                      <  2:623878/149‑1 (MQ=255)                                                                                  gcggcgATGACGTCAACCCCCAGACCGTTGGCGGCCCCTGCGTTCCCAGACCCGAGGCGCGCCGCAAAAAAAAGGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACt                                                                  <  2:1317562/151‑1 (MQ=255)                                                                                    ggcgATGACGTCAACCCCCAGACCGTTTGCGGCCCCTGCGTTACCAGACCCGAGGCGCCCCGCCAAAAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTg                                                                 <  2:713327/150‑1 (MQ=255)                                                                                    ggcgATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTgc                                                                >  2:90120/1‑151 (MQ=255)                                                                                    ggcgATGACGTAAACCTCCAGACCTTTTGCGGCCCCTGCGTTACCAGAACCGAGGCGCGCCCCAAAAAAAAGGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACt                                                                  <  2:1608421/149‑1 (MQ=255)                                                                                      cgATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGGGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCAACGAACGTTGATGCCGCACAACTgcgc                                                              >  1:288066/1‑151 (MQ=255)                                                                                      cgATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTgcgc                                                              >  2:162408/1‑151 (MQ=255)                                                                                      cgATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTgcgc                                                              >  2:209869/1‑151 (MQ=255)                                                                                       gATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGcgcc                                                             >  1:1541140/1‑151 (MQ=255)                                                                                        aTGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGcgccg                                                            >  1:1701351/1‑151 (MQ=255)                                                                                            cGTCAACCTCCAGACCGTTTGCGGCCCCTGCGTTACCAGAACCGAGGCGCGCCGCAAAAAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGcgccgc                                                           <  2:1512434/148‑1 (MQ=255)                                                                                             gTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGt                                                        >  1:173224/1‑150 (MQ=255)                                                                                              tCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGt                                                        >  2:1187523/1‑149 (MQ=255)                                                                                              tCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGt                                                        >  2:679245/1‑149 (MQ=255)                                                                                                aacccccAGCCCTTTTGCGCCCCCTGCTTTCCCAAACCCGGGGCGCCCCGCAAAAAAAATGCCGGC‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTa                                                       <  2:2966/143‑1 (MQ=255)                                                                                                aacccccAGCCCTTTGGCGCCCCCTGCTTTCCCAGACCCGGGGCGCGCCGCAAACAAAATGCCGTT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTa                                                       <  2:259339/143‑1 (MQ=255)                                                                                                aaaCTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGc                                                     >  1:1511851/1‑150 (MQ=255)                                                                                                 acccccggcccGTTGGCGGCCCCGGCTTCCCCAGACCCGGGGCGCGCCGCAAAAAAAATGCGGGC‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCAt                                                   <  2:984103/142‑1 (MQ=255)                                                                                                   ccccAGCCCGTTTGCGGCACCTGCGTTCCCAGACCCGAGGCGCGCCGCCAAAAAAAGGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTa                                                 <  2:711153/149‑1 (MQ=255)                                                                                                      cAGCCCTTTGGCGGCCCCGGCGTTACCAGACCCGAGGCGCGCCGCCAAAAAAATGCCGTT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCg                                              <  2:866603/151‑1 (MQ=255)                                                                                                          ccGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCa                                           >  2:686037/1‑150 (MQ=255)                                                                                                           cGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAAc                                         >  2:1428881/1‑151 (MQ=255)                                                                                                             tttGCGCCCCCTGCGTTCCCAAACCCGGGGCGCCCCCCAAAAAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACg                                        <  2:413818/150‑1 (MQ=255)                                                                                                             ttggcgcccccggCGTTCCCAGACCCGGGGCGCCCCCCAAAAAAAATGCCGTT‑CCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAAc                                         <  2:1223228/137‑1 (MQ=255)                                                                                                                   cccccggCGTTCCCAAACCCGGGGCGCGCCGCCAAAAAAATGCCGGT‑CCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCaaaaa                                 <  2:456799/145‑1 (MQ=255)                                                                                                                     cccTGCGTACCCAGACCCGGGGCGCCCCCCAAAAAAAATCCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCaaaaa                                 <  1:990332/148‑1 (MQ=255)                                                                                                                        tGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTACAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCg                              >  1:1616823/1‑149 (MQ=255)                                                                                                                          cGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAgcg                          >  2:443606/1‑151 (MQ=255)                                                                                                                           gTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAgcgc                         >  2:95073/1‑151 (MQ=255)                                                                                                                              aCCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACACCTGCGCCGCCGTAGCATTCCCGCCAACGGCAAAAACCAAGCGcc                        >  2:78091/1‑149 (MQ=255)                                                                                                                                 aGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGa                     >  2:338978/1‑149 (MQ=255)                                                                                                                                     ccGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCgg               >  1:439544/1‑151 (MQ=255)                                                                                                                                        aGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGttt            >  1:177398/1‑151 (MQ=255)                                                                                                                                         ggccccccgcaAAAAAAAGGCCGTC‑CCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGtttt           <  1:100299/145‑1 (MQ=255)                                                                                                                                         ggCGCGCCCCAAAAAAAAGGCCGTT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGttt            <  2:1576897/150‑1 (MQ=255)                                                                                                                                          gcgcgcCGAAAAAAAAAGGACGGT‑TACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGttt            <  1:216695/149‑1 (MQ=255)                                                                                                                                           cgcgcCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGc         >  1:1347501/1‑151 (MQ=255)                                                                                                                                            gcgcCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCg        >  1:1543112/1‑151 (MQ=255)                                                                                                                                            gcgcCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCg        >  2:1215491/1‑151 (MQ=255)                                                                                                                                                ccccAAAAAAAAGGCGGTCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCgg       <  1:230987/147‑1 (MQ=255)                                                                                                                                                   caacaaAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCtgat  >  1:957982/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                    AGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGAT  >  NC_000913/2675957‑2676252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑