Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,678,039	0	T	G	47.0%	95.4 / 18.2	66	N108T (AAC→ACC)	yphE	putative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (4/31);  new base G (31/0);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 8.34e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCT  >  NC_000913/2677890‑2678187                                                                                                                                                      |                                                                                                                                                      ccaGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGt                                                                                                                                                       <  2:57558/150‑1 (MQ=255)  caGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTCAACGAGGCAGAGGgtt                                                                                                                                                     >  1:126000/1‑151 (MQ=255)  caGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGAGGGAGAGGgtt                                                                                                                                                     >  1:604175/1‑151 (MQ=255)  caGCTGCTTTTGCGCCGGGCTTAGCGCTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCGAACCAGGCAGAGGgtt                                                                                                                                                     >  2:1672415/1‑151 (MQ=255)   aGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGtttt                                                                                                                                                    <  1:194511/151‑1 (MQ=255)    gctgctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGACCGAGGCCGAGGgtttt                                                                                                                                                   >  1:88861/1‑150 (MQ=255)    gctgctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCCAGGGCGAGGgttt                                                                                                                                                    >  1:647405/1‑150 (MQ=255)             gcgcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCCAGGGAGAGGgtttttccccc                                                                                                                                             >  1:1668369/1‑141 (MQ=255)              cgcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTc                                                                                                                                         <  2:242136/151‑1 (MQ=255)               gcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGGCCCAGGCAGAGGGGTTTCCCCACTGt                                                                                                                                          >  1:1149027/1‑149 (MQ=255)                ccGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAg                                                                                                                                       <  2:402192/151‑1 (MQ=255)                 cGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑c                                                                                                                                     <  2:339636/151‑1 (MQ=255)                   ggCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCCCGGCCGAGGGTTTTCGCCCCCGTCCG‑ccc                                                                                                                                   >  1:1579736/1‑151 (MQ=255)                        aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTcc                                                                                                                               <  2:57328/150‑1 (MQ=255)                          cGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACTGACCGAGGCAGAGGGTTTTCGCCACTGTCAG‑CCCTTccacc                                                                                                                            >  2:178646/1‑151 (MQ=255)                          cGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTTACCGAGGCAGAGGGTTTTCGCCACTGTCCG‑CCCCTccccc                                                                                                                            >  2:221020/1‑151 (MQ=255)                            ttGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCCCCCCCGTCAG‑CCCTTccccc                                                                                                                            >  2:818019/1‑149 (MQ=255)                             tGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAg                                                                                                                          <  2:251603/150‑1 (MQ=255)                               aaaCAAGTTGTTCAGGCCTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAg                                                                                                                          <  1:907814/148‑1 (MQ=255)                                 aCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGCGGCAGAGGGTTTTCGCCCCTGTCAG‑CCCCTCCACCCGACtt                                                                                                                      >  2:1033902/1‑150 (MQ=255)                                 aCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTGCCGCCACTGTCAG‑CCCTTCCACCAGACTTa                                                                                                                     <  1:300584/151‑1 (MQ=255)                                  cAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTaa                                                                                                                    <  2:1594258/151‑1 (MQ=255)                                   aaGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTaa                                                                                                                    <  2:1231217/150‑1 (MQ=255)                                    aGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACt                                                                                                                  <  1:1127095/151‑1 (MQ=255)                                    aGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCGCCACTGTCAG‑CCCTTCCCCCAGACTTaa                                                                                                                    >  2:1070877/1‑149 (MQ=255)                                    aGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCGCCACCGTCAG‑CCCTTCCCCCAGACttta                                                                                                                    >  2:129421/1‑147 (MQ=255)                                            aGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCAATGACCGAGGGAGAGGGTTTTCGCCCCTGTCAG‑CCCTTCCACCAGACCTAACTCCTGa                                                                                                             >  2:834861/1‑148 (MQ=255)                                            aGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGCAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGCGGCAGAGGGTTTCCGCCCCTGTCAG‑CCCCTCCCCCCCACTTAACTCCTGAt                                                                                                            >  1:1205727/1‑149 (MQ=255)                                             ggACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATaaa                                                                                                         <  1:1045572/151‑1 (MQ=255)                                                 tAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGGAGAGGGTTTTCGCCACTGTCAG‑CCCCTCCCCCACACTTAACTCCCGATaaa                                                                                                         >  2:490458/1‑147 (MQ=255)                                                  aaCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACcgcg                                                                                                    <  2:1312540/151‑1 (MQ=255)                                                  aaCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGCGGCGGAGGGTTTTCGCCCCTGCTAG‑CCCCTCCCCCCGCCTTAACACCTGATaaa                                                                                                         >  1:313073/1‑146 (MQ=255)                                                     gTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCCAGGCAGAGGGTTTTCGCCCCCGTCAG‑CCCCTCCACCCGACTTAAATCCCGATAAACcgcgc                                                                                                   >  1:1672602/1‑149 (MQ=255)                                                        aaCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGAACGCGGCAGAGGGTTTTCGCCCCCGTCAA‑CCCTTCCACCAACCTTAAATCCTCATAAAACCCCCGCAcc                                                                                              >  1:1103327/1‑151 (MQ=255)                                                             ccAGCGCCTGTAAGCAGCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCAGGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAg                                                                                         <  1:1676357/151‑1 (MQ=255)                                                             ccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGAGCCAGGCAGAGGGTTTCCCGCCCTGTCAA‑CCCCTCCACCCGAATTAAATCCCGATAAAACGCGCGCAccccc                                                                                           >  2:914769/1‑149 (MQ=255)                                                               aGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCGCCCCCGTCAG‑CCCTTCCACCAGACTTAAATCCTGATAAACCGCGCGCAccccccggt                                                                                       >  2:614091/1‑147 (MQ=255)                                                                    cTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCCGAGGGTTTTCGCCACTGTCAG‑CCCTTCCCCCAGACTTAACTCCTGATAAACCCCGCGCCCCCCCAGTTCAGCg                                                                                  >  2:1490226/1‑151 (MQ=255)                                                                     tGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGGGGCAGGGGGTTTCCGCCCCCGTCAG‑CCCTTCCCCCCCACCTTACTCCTGGTAAAACGCGCGCccccccccttccac                                                                                   >  1:444914/1‑141 (MQ=255)                                                                        aaGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCAc                                                                              <  1:26246/151‑1 (MQ=255)                                                                        aaGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCAc                                                                              <  1:1590550/151‑1 (MQ=255)                                                                        aaGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCTACCCCGGGCGAGGGTTTTCGCCCCTGTCAG‑CCCCTCCACCCCACCTAAATCCTGCTAAAACCCGCccccccccccgt                                                                                       >  1:1672542/1‑138 (MQ=255)                                                                         aGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGGCCCAGGCAGAGGGTTTTCGCCACTGTCAG‑CCCCTCCCCCACACTTAACTCCTGATAAACCGCGCGCCCCCCCCGCTCAGCGGccc                                                                              >  1:556490/1‑148 (MQ=255)                                                                           cAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACgg                                                                            <  1:602762/150‑1 (MQ=255)                                                                           cAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAAGCAGAGGGGTTTCCCCCCGCACCG‑CCCTTCCCCCCGACTTAAATCCTGATAAAACGCGCGCCCCCCCCGGTCacccggccg                                                                             >  2:1647416/1‑141 (MQ=255)                                                                            aaCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAAACGCGCGCACCCCCCGTTCAGCgg                                                                                 >  2:984719/1‑144 (MQ=255)                                                                                 tGGGCATCCTGCGCCATTTGCAGGAAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTc                                                                      <  2:1277380/150‑1 (MQ=255)                                                                                     cATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCGCGGCAGAGGGTTTTCCCCACCGTCCG‑CCCCTCCCCCCCACTTAAATCCTTATAAACCCCCCCCACCCCCCGTTCAACGGCACCGCGAGTCacc                                                                   >  2:822968/1‑147 (MQ=255)                                                                                       tCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGCCCCAGGCCGAGGTTTTTCCCCACCGTCAG‑CCCCTCCACCCGACTTAACTCCTGGTAAACCCCGCGCACCCCCCCTTCAGCGGCCCCGCGAGTCAGCg                                                                  >  1:302499/1‑148 (MQ=255)                                                                                          tGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGGAGAGGGTTTTCGCCCCTGTCAG‑CCCTTCCACCCGACTTAAATCCTGATAAAACGCGCGCACCCCCCGTTCAGCGGCACGGCGAGTCagcttagctt                                                            >  2:131281/1‑151 (MQ=255)                                                                                          tGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCGCCCCCGTCAG‑CCCTTCCACCCCACTTAACTCCCGATAAAACGCCCGCACCCCCCCTTCAGCGGCACCGCGGGTCAGCGCAGc                                                              >  1:1096144/1‑149 (MQ=255)                                                                                                  ttGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCGACCGAGGCCGAGGGTTTTCGCCCCTGTCCGTCCCTTCC‑CCACACTTAACACCTGTTAAACCGCCCCCACCCCCCCGTCAGCGGCACGGCGGGGCACGGGAGCTTCGTCAcc                                                     >  1:176941/1‑150 (MQ=255)                                                                                                    gCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCtt                                                   <  1:284158/150‑1 (MQ=255)                                                                                                      aGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCTAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCa                                                <  1:659934/151‑1 (MQ=255)                                                                                                          aatcaatcATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTcg                                            <  1:1007672/151‑1 (MQ=255)                                                                                                          aatcaatcATGCCGTTGCGGCGGGGCCACTGACCCAGGCAGAGGGTTTTCCCCCCCCTCAG‑ACCTTCCCCCCGACTTAAATCCTGATAAAACCCGCGCACCCCCCCTTCAACGGGACGGCGAGGCAACGTAGCCTCGTCACCCTcccgcc                                             >  1:1233782/1‑146 (MQ=255)                                                                                                               atcaTGCCGTTGCGGCGGGGCCACTGACCCAGGGAGAGGGTTTTCGCCCCTGTCAG‑CCCCTCCCCCCCACTTAACTCCTGATAAAACCCGCGCACCCCCCGTTCAGCGGCACCGGGGGGCAGCGGAGCTTCGTCACCCTcccctc                                             >  1:1148811/1‑141 (MQ=255)                                                                                                                tcaTGCCGTTGCGGCGGGGCCCCAGACCGAGGGAGAGGGTTTTCCCCCCTGTCAG‑CCCCTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCCCTTCAGCGGCACGGCGAGGCAGCGGAGCTTCGTCACCCTCCCGTCGCGgctc                                      >  1:887460/1‑151 (MQ=255)                                                                                                                 caTGCCGTTGCGGCGGGGCCCCCGACCCGGGCAGAGGGTTTTCGCCCCCGTCCG‑CCCCTCCCCCCGACTTAACTCCTGATAAAACGCGCGCACCCCCCGGTCAGCGGGACGGCGAGTCAGCGGAGCTTCGTCACCCTCCCGt                                              >  1:421638/1‑142 (MQ=255)                                                                                                                 caTGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGGCTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCg                                     <  1:948509/151‑1 (MQ=255)                                                                                                                 caTGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGtctc                                      <  1:1329503/150‑1 (MQ=255)                                                                                                                  aTGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGtctc                                      <  1:59480/149‑1 (MQ=255)                                                                                                                   tGCCGTTGCGGCGGGGCCACTCACCGAGGGAGAGGGTTTCCGCCACTGTCCG‑CCCCTCCCCCAGACTTAAATCCTGATTAACCACCCGCACCCCCAGCTCTGCGGCACGGCGGGGCAGCCGAGCTTCCTCACCTTCCAGTCCCGTCCCGcc                                   >  1:23328/1‑151 (MQ=255)                                                                                                                           cggcggGGCCCCTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAAACGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCCCCTTCCAGTCGCGTCTCGCCAATcc                              >  2:530247/1‑148 (MQ=255)                                                                                                                              cggGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGatat                        >  1:1418507/1‑151 (MQ=255)                                                                                                                                      cTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCt                 <  1:656533/150‑1 (MQ=255)                                                                                                                                        gACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATc              <  1:796730/151‑1 (MQ=255)                                                                                                                                          ccGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCg            <  1:921527/151‑1 (MQ=255)                                                                                                                                           cGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCgg           <  1:723496/151‑1 (MQ=255)                                                                                                                                               gCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGt       >  2:453465/1‑151 (MQ=255)                                                                                                                                                  gggattttCCGCCACTGTCAG‑CCCCGCCGCCAGACTTAACTCCGGATAAGCCGCGCACACCCCCAGTTCAGCGGCACGGGGAGTTAGCGTAGCTTCGTCACCTTCCAGTGGCGTCTCGCCAATCCAGACATCACCGCTATCCGGGCGtt      <  1:1185999/145‑1 (MQ=255)                                                                                                                                                  gagTTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTc     <  2:542621/150‑1 (MQ=255)                                                                                                                                                    ggTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCt  <  2:4346/151‑1 (MQ=255)                                                                                                                                                    ggTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCt  <  1:746049/151‑1 (MQ=255)                                                                                                                                                    ggTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCt  <  2:928715/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      CCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAG‑CCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCT  >  NC_000913/2677890‑2678187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑