Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,335,317 T→G Y393S (TAT→TCT)  murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,335,3170TG99.0% 413.2 / NA 103Y393S (TAT→TCT) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (48/54);  total (49/54)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

CACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCGGCG  >  NC_000913/3335169‑3335463
                                                                                                                                                    |                                                                                                                                                  
cacTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGaa                                                                                                                                                  <  2:1420593/151‑1 (MQ=255)
 acTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGaaa                                                                                                                                                 <  1:1449561/151‑1 (MQ=255)
   tAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAAt                                                                                                                                                >  1:1671332/1‑150 (MQ=255)
    aaaCCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATAc                                                                                                                                              <  1:1165417/151‑1 (MQ=255)
     aaCCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACg                                                                                                                                             >  1:809028/1‑151 (MQ=255)
     aaCCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACg                                                                                                                                             <  1:138551/151‑1 (MQ=255)
        cGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATc                                                                                                                                          >  1:317362/1‑151 (MQ=255)
         gAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCCCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACg                                                                                                                                             >  1:1583670/1‑147 (MQ=255)
          aaaaaaTGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCa                                                                                                                                         >  1:1111865/1‑150 (MQ=255)
            aaaaTGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTACTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAacc                                                                                                                                      <  2:1343013/151‑1 (MQ=255)
             aaaTGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAacca                                                                                                                                     <  2:1409880/151‑1 (MQ=255)
             aaaTGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAacc                                                                                                                                      >  1:1525456/1‑150 (MQ=255)
              aaTGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAaccac                                                                                                                                    >  1:1254844/1‑151 (MQ=255)
              aaTGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAaccac                                                                                                                                    >  2:157197/1‑151 (MQ=255)
                   gTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACcgtcg                                                                                                                               >  2:1410414/1‑151 (MQ=255)
                   gTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACcgtcg                                                                                                                               <  1:648708/151‑1 (MQ=255)
                     ggTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACcgtcgt                                                                                                                              <  2:1623180/150‑1 (MQ=255)
                         tGGCGGTAGCCCCGCGAAAGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCCCACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCtt                                                                                                                         >  1:257128/1‑151 (MQ=255)
                           gCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTcc                                                                                                                       <  2:1109931/151‑1 (MQ=255)
                           gCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCACTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCACCCACCGTCGTCCCTTcc                                                                                                                       <  1:547951/151‑1 (MQ=255)
                           gCGGGAGACCCGGGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTATCACACGCTCAATAGTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTcc                                                                                                                       >  1:300400/1‑151 (MQ=255)
                               tAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCa                                                                                                                    >  2:1104409/1‑150 (MQ=255)
                               tAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCa                                                                                                                    >  2:1409939/1‑150 (MQ=255)
                               tAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCa                                                                                                                    >  2:187680/1‑150 (MQ=255)
                                 gCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATa                                                                                                                 <  2:1237166/151‑1 (MQ=255)
                                 gCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATa                                                                                                                 <  1:1375524/151‑1 (MQ=255)
                                    ccGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATAc                                                                                                                >  1:1650146/1‑149 (MQ=255)
                                       cgAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATAcagcc                                                                                                            >  1:1567389/1‑150 (MQ=255)
                                        gAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATAcagccag                                                                                                          <  1:944281/151‑1 (MQ=255)
                                         aaCGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATAcagccagc                                                                                                         >  1:906285/1‑151 (MQ=255)
                                         aaCGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATAcagccagc                                                                                                         <  2:517201/151‑1 (MQ=255)
                                           cGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATAcagccagcca                                                                                                       <  2:1238642/151‑1 (MQ=255)
                                           cGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATAcagccagcca                                                                                                       <  1:685678/151‑1 (MQ=255)
                                             gggCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATAcagccagccagc                                                                                                     <  1:802634/151‑1 (MQ=255)
                                              ggCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATAcagccagccagc                                                                                                     >  2:1003370/1‑150 (MQ=255)
                                               gCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCAc                                                                                                   >  1:1106457/1‑151 (MQ=255)
                                                  gCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAg                                                                                                <  2:749934/151‑1 (MQ=255)
                                                   ccAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTTAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAgg                                                                                               <  1:713718/151‑1 (MQ=255)
                                                     aGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGc                                                                                              >  1:1616363/1‑150 (MQ=255)
                                                      gCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCtt                                                                                            <  1:1453713/151‑1 (MQ=255)
                                                          tcAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTg                                                                                        >  2:854081/1‑151 (MQ=255)
                                                            aGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGAt                                                                                      <  2:49871/151‑1 (MQ=255)
                                                             gACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATg                                                                                     <  1:450743/151‑1 (MQ=255)
                                                             gACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATg                                                                                     <  2:250410/151‑1 (MQ=255)
                                                              aCGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGc                                                                                    <  1:659169/151‑1 (MQ=255)
                                                                gATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCAc                                                                                  <  2:1651955/151‑1 (MQ=255)
                                                                  ttattCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGc                                                                                >  1:332485/1‑151 (MQ=255)
                                                                   tattCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGc                                                                                >  2:1152035/1‑150 (MQ=255)
                                                                   tattCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGc                                                                                >  1:1178602/1‑150 (MQ=255)
                                                                       cGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGAt                                                                            <  2:1660163/150‑1 (MQ=255)
                                                                       cGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATc                                                                           <  2:1092761/151‑1 (MQ=255)
                                                                         ccTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGc                                                                                >  2:761052/1‑144 (MQ=255)
                                                                          cTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGt                                                                        >  1:1225704/1‑151 (MQ=255)
                                                                             tCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTg                                                                      <  1:967983/150‑1 (MQ=255)
                                                                               acacGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGcc                                                                    >  2:916176/1‑150 (MQ=255)
                                                                                  cGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATa                                                                 <  2:1470616/150‑1 (MQ=255)
                                                                                  cGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATa                                                                 >  2:1538773/1‑150 (MQ=255)
                                                                                   gCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAAc                                                               >  1:1668106/1‑151 (MQ=255)
                                                                                      cAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCtg                                                            <  1:129154/151‑1 (MQ=255)
                                                                                      cAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCt                                                             <  1:646312/150‑1 (MQ=255)
                                                                                       aaTATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCtg                                                            >  2:1035779/1‑150 (MQ=255)
                                                                                        atatTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCtgtg                                                          <  1:562314/151‑1 (MQ=255)
                                                                                          atTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTgcg                                                        <  2:269532/151‑1 (MQ=255)
                                                                                              gCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAg                                                    <  1:1582681/151‑1 (MQ=255)
                                                                                               cACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGa                                                   <  1:678535/151‑1 (MQ=255)
                                                                                                 ccTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGaaa                                                 <  2:133097/151‑1 (MQ=255)
                                                                                                  cTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGaa                                                  >  1:353552/1‑149 (MQ=255)
                                                                                                   tAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGt                                               >  2:954589/1‑151 (MQ=255)
                                                                                                    agaGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGtt                                              <  1:417451/149‑1 (MQ=255)
                                                                                                    aaaGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAg                                                <  2:70034/149‑1 (MQ=255)
                                                                                                    aaaGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTACCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAg                                                <  1:1209148/149‑1 (MQ=255)
                                                                                                        cgcgcAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTc                                          >  2:171493/1‑151 (MQ=255)
                                                                                                         gcgcAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCa                                         <  1:332037/151‑1 (MQ=255)
                                                                                                             aGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAacac                                     >  2:1376513/1‑151 (MQ=255)
                                                                                                                ttGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCg                                   <  2:509707/150‑1 (MQ=255)
                                                                                                                 tGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGt                                  <  2:1090862/150‑1 (MQ=255)
                                                                                                                 tGTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTg                                 >  2:1462162/1‑151 (MQ=255)
                                                                                                                  gTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTg                                 <  1:1214887/150‑1 (MQ=255)
                                                                                                                  gTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGa                                <  1:953844/151‑1 (MQ=255)
                                                                                                                  gTCTTCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCACCAGAAAGTTTTTCAACACCGTg                                 <  1:1329887/150‑1 (MQ=255)
                                                                                                                      tCAATGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACaa                             >  1:160378/1‑150 (MQ=255)
                                                                                                                        aaTGCGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATa                          <  2:1030422/151‑1 (MQ=255)
                                                                                                                            cGTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACgg                      >  1:1072462/1‑151 (MQ=255)
                                                                                                                             gTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACgg                      >  1:47971/1‑150 (MQ=255)
                                                                                                                             gTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGt                     <  1:1156676/151‑1 (MQ=255)
                                                                                                                             gTTCGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGt                     <  2:214468/151‑1 (MQ=255)
                                                                                                                                cGTAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTAt                   <  1:1269102/150‑1 (MQ=255)
                                                                                                                                  tAGCCACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGc                >  2:961918/1‑151 (MQ=255)
                                                                                                                                      cACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTc            >  2:1400166/1‑151 (MQ=255)
                                                                                                                                      cACGATCGATGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTc            <  2:1222100/151‑1 (MQ=255)
                                                                                                                                        cgatcgatGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGa          <  2:679381/151‑1 (MQ=255)
                                                                                                                                          atcgatGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGAtt        >  1:1432571/1‑151 (MQ=255)
                                                                                                                                            cgatGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTc      <  2:1478802/151‑1 (MQ=255)
                                                                                                                                            cgatGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTc      >  2:1002529/1‑151 (MQ=255)
                                                                                                                                            cgatGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTc      >  1:1668628/1‑151 (MQ=255)
                                                                                                                                             gatGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCg     <  1:1604670/151‑1 (MQ=255)
                                                                                                                                             gatGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCg     >  1:621238/1‑151 (MQ=255)
                                                                                                                                              atGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCgg    >  2:236672/1‑151 (MQ=255)
                                                                                                                                              atGTGAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCgg    <  2:1254889/151‑1 (MQ=255)
                                                                                                                                               tgtgAGAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCGGc   >  1:859010/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTCCGCAATACAGCCAGCCAGCACCAGGCTTGCTGATGCACGCAGATCGGTTGCCATAACCTGTGCGCCAGAAAGTTTTTCAACACCGTGACAAATAACGGTATTGCTTTCGATTTCGGCG  >  NC_000913/3335169‑3335463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑