Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,807,248 +T coding (117/993 nt) proX → glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,807,2471.T99.1% 446.2 / ‑4.1 116coding (116/993 nt)proXglycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (71/44);  total (71/45)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGAC  >  NC_000913/2807101‑2807391
                                                                                                                                                   |                                                                                                                                                 
cATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACttga                                                                                                                                               <  1:261821/151‑4 (MQ=255)
 aTTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACttga                                                                                                                                               >  1:857770/1‑147 (MQ=255)
  ttATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACttga                                                                                                                                               >  2:1072759/1‑146 (MQ=255)
      cACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGaaa                                                                                                                                          <  2:1385309/150‑1 (MQ=255)
        cccTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAAcc                                                                                                                                        <  2:1327444/150‑1 (MQ=255)
         ccTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCtt                                                                                                                                      >  1:1351172/1‑151 (MQ=255)
            gCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTcc                                                                                                                                    <  2:58249/150‑1 (MQ=255)
              cAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTcc                                                                                                                                    >  2:59272/1‑148 (MQ=255)
              cAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGa                                                                                                                                 <  1:1004371/151‑1 (MQ=255)
               aaaaaaaGGAATAACAATGCAACATAGCGTGCTTTTTGCGACAGCGTTTGCCACGCTTATCTGTACACAAACTTTTGCGGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGAc                                                                                                                                <  2:174105/151‑1 (MQ=255)
                aaaaaaGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACg                                                                                                                               >  1:536586/1‑151 (MQ=255)
                 aaaaaGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACgc                                                                                                                              >  2:831896/1‑151 (MQ=255)
                  aaaaGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACgct                                                                                                                             <  1:144329/151‑1 (MQ=255)
                    aaGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACgctgc                                                                                                                           >  1:503883/1‑151 (MQ=255)
                      ggAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGctgctg                                                                                                                         >  1:88160/1‑151 (MQ=255)
                       gAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTgg                                                                                                                        >  1:700827/1‑151 (MQ=255)
                       gAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTgg                                                                                                                        <  2:86729/151‑1 (MQ=255)
                         aTAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTc                                                                                                                      <  1:409935/151‑1 (MQ=255)
                           aaCAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCa                                                                                                                     <  1:1068088/150‑1 (MQ=255)
                             cAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTc                                                                                                                  >  1:275858/1‑151 (MQ=255)
                             aaaggcaaCATAGCGTACTTTTTGCGACAGCGTTTGCCAGGCTTATCTCTACACAAACTTTTGCTGCCAATCTGCCGGGCAAAGGCATTACTGATAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGt                                                                                                                   >  2:295509/8‑150 (MQ=255)
                              aaTGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTc                                                                                                                  <  2:861376/150‑1 (MQ=255)
                               aTGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCg                                                                                                                 >  2:664272/1‑150 (MQ=255)
                               aTGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCg                                                                                                                 >  1:511790/1‑150 (MQ=255)
                               aTGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGt                                                                                                                >  2:578547/1‑151 (MQ=255)
                                 gCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTg                                                                                                               <  1:1210585/150‑1 (MQ=255)
                                 gCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTg                                                                                                               <  1:102975/150‑1 (MQ=255)
                                    aCATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCt                                                                                                           >  1:1265563/1‑151 (MQ=255)
                                     cATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTg                                                                                                          >  2:1521369/1‑151 (MQ=255)
                                     cATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTg                                                                                                          >  2:54673/1‑151 (MQ=255)
                                      aTAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTgg                                                                                                         >  2:1006879/1‑151 (MQ=255)
                                      aTAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTgg                                                                                                         >  1:273140/1‑151 (MQ=255)
                                        aGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTgg                                                                                                         >  1:94943/1‑149 (MQ=255)
                                        aGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTgg                                                                                                         >  1:737121/1‑149 (MQ=255)
                                        aGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGga                                                                                                        <  2:469324/150‑1 (MQ=255)
                                        aGCGTACTTTTTGCGACAGCGTTGGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCGCTGGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGga                                                                                                        <  2:471294/150‑1 (MQ=255)
                                         gCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTgg                                                                                                         >  2:1497041/1‑148 (MQ=255)
                                          cGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTACTGGTCAGTCGTGCGCTGgaga                                                                                                      >  2:448763/1‑150 (MQ=255)
                                             aCTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAAtt                                                                                                  >  2:1045254/1‑151 (MQ=255)
                                             aCTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAAtt                                                                                                  >  2:1285254/1‑151 (MQ=255)
                                             aCTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAAt                                                                                                   <  1:1406470/150‑1 (MQ=255)
                                              cTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCCGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAAtt                                                                                                  <  2:680815/150‑1 (MQ=255)
                                               tttttGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAAtt                                                                                                  >  2:1282086/1‑149 (MQ=255)
                                                   tGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGtt                                                                                             >  1:1224669/1‑150 (MQ=255)
                                                    gCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTta                                                                                            <  2:831828/150‑1 (MQ=255)
                                                    gCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTta                                                                                            <  1:1220018/150‑1 (MQ=255)
                                                     cGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTtat                                                                                           >  1:1229083/1‑150 (MQ=255)
                                                      gACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATAc                                                                                         >  1:639550/1‑151 (MQ=255)
                                                       aCAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATAcc                                                                                        <  1:309469/151‑1 (MQ=255)
                                                        cAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCg                                                                                       >  1:528678/1‑151 (MQ=255)
                                                        cAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCg                                                                                       >  1:1136785/1‑151 (MQ=255)
                                                          gCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTc                                                                                     >  2:963746/1‑151 (MQ=255)
                                                          gCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTc                                                                                     <  1:297072/151‑1 (MQ=255)
                                                           cGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTCCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTca                                                                                    <  2:393421/151‑1 (MQ=255)
                                                            gTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTca                                                                                    >  1:407479/1‑150 (MQ=255)
                                                              ttGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTcaac                                                                                  >  1:586201/1‑150 (MQ=255)
                                                               tGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTcaaca                                                                                 >  1:1478651/1‑150 (MQ=255)
                                                                 ccACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAAc                                                                              >  2:1043355/1‑151 (MQ=255)
                                                                 ccACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAAc                                                                              >  1:1111844/1‑151 (MQ=255)
                                                                 ccACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAAc                                                                              >  2:737865/1‑151 (MQ=255)
                                                                  cACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAAcc                                                                             <  2:1308077/151‑1 (MQ=255)
                                                                  cACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAAcc                                                                             <  1:797497/151‑1 (MQ=255)
                                                                   aCGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTTAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAAccc                                                                            >  2:1074346/1‑151 (MQ=255)
                                                                      cTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGc                                                                         >  1:254292/1‑151 (MQ=255)
                                                                       ttATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCg                                                                        >  1:1512516/1‑151 (MQ=255)
                                                                        tATCTCTACACACACTTTTGCTACCGAACTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGAGAGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCg                                                                        >  2:641734/1‑150 (MQ=255)
                                                                        tATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCg                                                                        >  2:917654/1‑150 (MQ=255)
                                                                        tATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCg                                                                        >  1:1337312/1‑150 (MQ=255)
                                                                         aTCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGa                                                                       <  1:644241/150‑1 (MQ=255)
                                                                             ctACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCAGCACTTGAAGAAGCCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGt                                                                    <  2:36163/149‑1 (MQ=255)
                                                                                cacaAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGAtt                                                               <  2:938097/151‑1 (MQ=255)
                                                                                 acaAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGAtt                                                               >  2:120108/1‑150 (MQ=255)
                                                                                  caAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTAc                                                             >  1:1387666/1‑151 (MQ=255)
                                                                                  caAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTAc                                                             <  1:301052/151‑1 (MQ=255)
                                                                                   aaaCTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACa                                                            <  1:1522385/151‑1 (MQ=255)
                                                                                     aCTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAAc                                                          <  1:1293703/151‑1 (MQ=255)
                                                                                          tgctgcCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTg                                                      <  1:1403272/150‑1 (MQ=255)
                                                                                           gctgcCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTgg                                                     >  2:1376772/1‑150 (MQ=255)
                                                                                           gctgcCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGc                                                    >  2:1522283/1‑151 (MQ=255)
                                                                                             tgcCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCt                                                   >  2:773567/1‑150 (MQ=255)
                                                                                                     tGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCgc                                          >  1:1041979/1‑151 (MQ=255)
                                                                                                      gCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTc                                            <  2:1179722/148‑1 (MQ=255)
                                                                                                      gCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCgct                                         <  1:926944/151‑1 (MQ=255)
                                                                                                       ccGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCgctt                                        <  1:1208498/151‑1 (MQ=255)
                                                                                                              aaGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCg                                 >  2:887642/1‑151 (MQ=255)
                                                                                                              aaGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCg                                 <  2:771191/151‑1 (MQ=255)
                                                                                                               aGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCg                                 >  2:37851/1‑150 (MQ=255)
                                                                                                                 gCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCg                              >  2:45611/1‑151 (MQ=255)
                                                                                                                  cATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCg                              >  2:677123/1‑150 (MQ=255)
                                                                                                                      aCTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCa                         <  2:400152/151‑1 (MQ=255)
                                                                                                                       cTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCa                         >  1:83737/1‑150 (MQ=255)
                                                                                                                           tAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCtt                    >  2:538905/1‑151 (MQ=255)
                                                                                                                            aaTCCAGATCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTc                   <  2:611986/151‑1 (MQ=255)
                                                                                                                                  gTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCAccgcc             <  1:959170/151‑1 (MQ=255)
                                                                                                                                    tCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGt           >  1:1467331/1‑151 (MQ=255)
                                                                                                                                     cAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTg          >  2:1455691/1‑151 (MQ=255)
                                                                                                                                     cAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTg          >  1:713568/1‑151 (MQ=255)
                                                                                                                                      agagCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTg          >  1:464311/1‑150 (MQ=255)
                                                                                                                                       gagCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGa         >  2:630267/1‑150 (MQ=255)
                                                                                                                                             cATCACTTGAAGAACCCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGAAGAAATTAGGTTATACCGTCAACAACCCCAGCGAAGTAGATTACAACGTGGGCTACCCCTCGCTTGCTTCCGGCGAGGCAACTTTCCCCGCCGTGAACTGGAc  <  2:1215451/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGAC  >  NC_000913/2807101‑2807391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: