Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4960TA42.1% 146.3 / 53.9 95intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (51/4);  new base A (0/40);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 1.36e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29348‑29642
                                                                                                                                                      |                                                                                                                                                  
tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAAtata                                                                                                                                                   >  1:1150777/1‑150 (MQ=255)
 aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATaa                                                                                                                                                  >  2:262198/1‑150 (MQ=255)
 aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATaa                                                                                                                                                  <  1:852657/150‑1 (MQ=255)
  aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATaaa                                                                                                                                                 >  1:682189/1‑150 (MQ=255)
     tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAAt                                                                                                                                             >  2:274593/1‑151 (MQ=255)
      aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAAt                                                                                                                                             <  1:972801/150‑1 (MQ=255)
      aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATc                                                                                                                                            >  1:303912/1‑151 (MQ=255)
      aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATc                                                                                                                                            >  2:188627/1‑151 (MQ=255)
           ttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGcc                                                                                                                                       >  2:193645/1‑151 (MQ=255)
            tGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGcc                                                                                                                                       <  1:67555/150‑1 (MQ=255)
             gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                                     >  1:972525/1‑151 (MQ=255)
               ccATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCAtt                                                                                                                                    >  2:1160179/1‑150 (MQ=255)
                cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                                   >  1:150267/1‑150 (MQ=255)
                cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                                   >  2:716398/1‑150 (MQ=255)
                  tttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGt                                                                                                                                >  2:1418282/1‑151 (MQ=255)
                   ttGCTCCTCTTTTTTCTCCTCTTTTTTTTTTCATGCAATTTTCTTGCGGCGCAAGCGTTTCCAAAAACAG‑TTTAGAGGATCTTTTTGTCGCTAAATCCTTGAAAACCATGCAT‑GACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGt                                                                                                                                <  1:796780/150‑1 (MQ=255)
                    tGGTCCTTTTTTTTCTGCTCTTTTTTTTTTCATGCATTCTTCTTGCTGCGCAAGCTTTTTCAAGAACAG‑GTTAGATAATTTTTTTGTCGTTTAATGCCTAAAAAACATGCAT‑GAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGtt                                                                                                                               <  2:869464/150‑1 (MQ=255)
                     ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                              >  2:139544/1‑150 (MQ=255)
                            ttttttCTGCTTTTTTTTATTTCAGGAATTTTTTTTGCTGCGCAAGCTTTTTCCAGAACGG‑GTAAGATGTTTTTTTTTTCTCTTAATGCCTGAAAAACATGCAT‑GAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                         <  1:564655/148‑1 (MQ=255)
                            ttttttCGGCTTTTTTTTATTTCATGAAATCTTCTGGCTGCGCAAGCTTTTCCAAAAACAG‑GTTAGATGTTTTTTTTGTCGCTTAATCCCTGAAAAACATGCAG‑GAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                        <  1:241464/149‑1 (MQ=255)
                             tttttCTCCTCTTTTTTATTTAATGAATTTTTCTTGCTGCGAAAGCTTTTTCCAGAACAG‑GTAAAATGATTTTTTTGTCGTTAAATGCCTGTAAAACTTGCAT‑GACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAg                                                                                                                      <  2:462501/150‑1 (MQ=255)
                                  cTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                 >  2:1128890/1‑150 (MQ=255)
                                      tttttttttttCCATGCATTTTTCTTGCTGCCCAAGCTTTTCCAAAAACGG‑TTTAAATTATTTTTTTGTATCTTAGTTCATAT‑AAACATTCCTGGACCCACAAAATAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                               <  2:1212835/145‑1 (MQ=255)
                                        gTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                          >  1:169359/1‑151 (MQ=255)
                                         tttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         >  1:145710/1‑151 (MQ=255)
                                         tttttATTTCATGAAATCTTCTTGCTGCGCAAGCGTTTCCCAAACCAG‑TTAAGATGATTTTTTTGTCTTTTAATGCCTGAAAAACAGGCAG‑GAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                             <  1:77249/147‑1 (MQ=255)
                                          ttttATTTCATACAATCTTCTTGCTGCTCAAGCTTTTCCCAAAAAAG‑GTAAGATAATTTTTTTGTCGCTTAATGCCTAAAAAACATGCAT‑GAGCCACAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         <  2:714254/150‑1 (MQ=255)
                                             tATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                      >  2:803447/1‑150 (MQ=255)
                                                 tCATGCATTTTTCTTGCTGCGCAAGCTTTTTCCAAAACAG‑GTTAGATGTTTTTTTTGTCGCTTAATGCCTGAAAAACATGCAT‑GAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                                  <  1:905882/150‑1 (MQ=255)
                                                   atgaatttttctcGCGGCGCATGCTTTTCCCAAAACGG‑TTTAAATGTTTTTTTTTGCTTTTAATGCCTTAAAAACATGCAT‑GACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                                <  2:1109680/142‑1 (MQ=255)
                                                    tGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                              >  1:1028327/1‑151 (MQ=255)
                                                     gCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGaa                                                                                                     >  1:1343644/1‑143 (MQ=255)
                                                      cAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGt                                                                                            >  2:303850/1‑151 (MQ=255)
                                                        aTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                          >  2:1169730/1‑151 (MQ=255)
                                                        aTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                          >  1:810654/1‑151 (MQ=255)
                                                          cttcttGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGc                                                                                        >  1:73498/1‑151 (MQ=255)
                                                          cttcttGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGc                                                                                        >  2:870237/1‑151 (MQ=255)
                                                           ttcttGCTGCGAAAGCTTTTACCAAAAAGG‑GTTAGATGATTTTTTTGTCGTTAAAGGCCTATAAAACATGCAT‑GCGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTg                                                                                         <  2:573538/149‑1 (MQ=255)
                                                            tcttGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                       >  2:1529945/1‑150 (MQ=255)
                                                              ttGCTGCGAAAGCTTTTTCCAGAACAG‑TTTAGATGTTTTTTTTGTCGTTTAATGCCTGAAAAACATGCAG‑GAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTg                                                                                         <  1:396180/146‑1 (MQ=255)
                                                               tgctgcGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                                    >  1:713494/1‑150 (MQ=255)
                                                                gctgcGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                   >  2:1348596/1‑150 (MQ=255)
                                                                gctccGCAAGCTTTTTCAAAAAAAT‑TTTATATTATTTTTTTGCCTCTAAATGCCTAAAAAACTTGCAT‑GACCCAAAAAATAAAAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                   <  2:1042759/150‑1 (MQ=255)
                                                                 ctgcGAAAGTGTTTTCAAGAACAG‑GTTAGATGATTTTTTTTTCGTTTAATGCCTGAAAAACATGCAT‑GACCCACAAAATAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAg                                                                                     <  2:285664/147‑1 (MQ=255)
                                                                  tgcGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                >  2:52537/1‑151 (MQ=255)
                                                                  tgcGAAAGCG‑TTTCCAAAACCGTGTTAGATGATTTTTTTGTCGTTTAATGCCTGAAAAACATGCAT‑AACCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaaa                                                                                  <  2:615610/149‑1 (MQ=255)
                                                                  gccgaaggctttttCAAAAAAAG‑GTTAGTTAATTTTTTTGCCGCTTAATGCTTAAAAAACATCTGA‑GCGCAAAAAAATAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                <  2:648620/141‑1 (MQ=255)
                                                                    cgcAAGCTTTTTCCAGAACAG‑TTAAGTTGATTTTTTTGTCGTTTATTCCCTAAAAAACATGCAT‑GACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAt                                                                                 <  1:849845/148‑1 (MQ=255)
                                                                      cAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                            >  1:1393798/1‑151 (MQ=255)
                                                                            ttttCAAAAACAG‑GTTAGATAATTTTTTTGTCGTTTATTCCTTTAAAAACATGCTG‑GGGCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                         <  2:1496720/148‑1 (MQ=255)
                                                                            tttcCCAGAACAG‑GTTAGATGTTTTTTTTGTCGTTAAATGCCTGAAAAACTTGCAT‑GACCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                                       <  1:438337/150‑1 (MQ=255)
                                                                            tttacaaGAACAG‑GTTAGATGTTTTTTTTGTCGTTTAAGCCCTGAAAAACATGAAT‑GAGCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                        <  2:1125289/143‑1 (MQ=255)
                                                                                caaaaaCAG‑GTTAGATGATTTTTTTGTCGTTTAATGCTTAAAAAACATGCAT‑GACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                                  <  2:573309/147‑1 (MQ=255)
                                                                                  aGAACAG‑GTTAGATGATTTTTTTGTCGTTAAATGCCTGAAAAACATGCAT‑GACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                    <  1:189201/147‑1 (MQ=255)
                                                                                   aaaaaG‑GTTAGATATTTTTTTTGTCGTTAAATCCCTAAAAAACATGCAT‑GACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                               <  2:1080071/147‑1 (MQ=255)
                                                                                    aaCAG‑GTTAGATGTTCTTTTTGTCGCTTAATGCTTGAAAAACATGCAT‑GACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAtt                                                                <  1:354864/149‑1 (MQ=255)
                                                                                    aaCAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                              >  1:1321040/1‑151 (MQ=255)
                                                                                        g‑gTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                          >  2:1035103/1‑151 (MQ=255)
                                                                                           ttAGATGATTTTTTTGTCTTTTAATCCCTGAAAACCATGCTT‑GACCAAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                         <  2:16858/150‑1 (MQ=255)
                                                                                            tAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAt                                                       >  2:1161222/1‑151 (MQ=255)
                                                                                            tAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAt                                                       >  1:772278/1‑151 (MQ=255)
                                                                                               atgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                                     >  2:1132525/1‑150 (MQ=255)
                                                                                                tgttttttttGTCGTTTAATCCCTGAAAAACATGCAT‑GACCCAAAAAATAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                           <  2:58214/138‑1 (MQ=255)
                                                                                                     tttttGTCTTTTAATCCTTAAAAAACATGCAT‑GACCCAAAAAAAAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                <  2:83350/149‑1 (MQ=255)
                                                                                                     tttttGTCGCTTAATGCCTGAAAAACAGGCAT‑GAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtca                                               <  1:650741/150‑1 (MQ=255)
                                                                                                      ttttGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                             >  2:1314615/1‑151 (MQ=255)
                                                                                                           tCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAGTATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                         >  2:1402566/1‑150 (MQ=255)
                                                                                                            cgtttatgccagaaaaaaTATGCAT‑CACCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                        <  2:553981/136‑1 (MQ=255)
                                                                                                            cgtttAATCCTTGAAAAACATGCAT‑GACCCAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                       <  1:841097/148‑1 (MQ=255)
                                                                                                             gCTTAATGCCTGTAAACCATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                       >  1:570673/1‑150 (MQ=255)
                                                                                                              cTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTCGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                      >  1:1026475/1‑150 (MQ=255)
                                                                                                               ttAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                    >  1:483115/1‑151 (MQ=255)
                                                                                                                 aaTGCTTGAAAAACATGCAG‑GAGCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                                     <  1:1293157/148‑1 (MQ=255)
                                                                                                                  aTGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                  >  1:185635/1‑150 (MQ=255)
                                                                                                                    gCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                >  2:40005/1‑150 (MQ=255)
                                                                                                                     cttaaaaaaCATGCAG‑GACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                         <  2:1381052/135‑1 (MQ=255)
                                                                                                                       tGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGc                            >  1:299184/1‑151 (MQ=255)
                                                                                                                          aaaaaaTGCAT‑GAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaaa                         <  1:913015/151‑1 (MQ=255)
                                                                                                                          aaaaCATGCAT‑GAGCCACAAAATAATATAAAAAATCAAGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGACTTGATTTACGTCATCATTGTGAATTAATATGCa                           >  2:994848/1‑149 (MQ=255)
                                                                                                                          aaaaCATGCAG‑GACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                           <  1:1149541/149‑1 (MQ=255)
                                                                                                                          aaaaCAGGCAT‑GACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                             <  1:1160231/147‑1 (MQ=255)
                                                                                                                           aaaCATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaaa                         >  2:21194/1‑150 (MQ=255)
                                                                                                                           aaaCATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                        >  2:678826/1‑151 (MQ=255)
                                                                                                                                tgcat‑gAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                   >  2:1209069/1‑151 (MQ=255)
                                                                                                                                 gcat‑gAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                   >  2:1469240/1‑150 (MQ=255)
                                                                                                                                    t‑gAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt               >  2:15333/1‑151 (MQ=255)
                                                                                                                                      gacccAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                  <  1:394254/144‑1 (MQ=255)
                                                                                                                                      gacccAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga                 <  1:768543/145‑1 (MQ=255)
                                                                                                                                      gaccaaaaaaaaaATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga                 <  2:91560/141‑1 (MQ=255)
                                                                                                                                       acccAAAAAATAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAgtgagtga             <  1:1398568/149‑1 (MQ=255)
                                                                                                                                       aGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg              >  2:573104/1‑150 (MQ=255)
                                                                                                                                       aGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg              >  2:1334664/1‑150 (MQ=255)
                                                                                                                                        gCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAgtgagtga             >  2:991661/1‑150 (MQ=255)
                                                                                                                                           aaaaaaaaataaaaaaatTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat         <  2:682161/149‑1 (MQ=255)
                                                                                                                                            aaaaaaaATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt        <  1:1106036/150‑1 (MQ=255)
                                                                                                                                              aaaaaaTAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct      <  1:1399838/151‑1 (MQ=255)
                                                                                                                                               aataatATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtctc     >  1:458375/1‑151 (MQ=255)
                                                                                                                                                   atatAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg  >  2:328778/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
TAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAG‑GTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29348‑29642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑