Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	213,341	0	A	G	42.2%	120.2 / 29.2	83	G337G (GGA→GGG)	tilS	tRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (8/40);  new base G (35/0);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 4.74e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGC  >  NC_000913/213191‑213485                                                                                                                                                       |                                                                                                                                                   aGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGa                                                                                                                                                    <  2:1531587/151‑1 (MQ=255)     gCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAgt                                                                                                                                                 <  2:295675/150‑1 (MQ=255)            ccTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGc                                                                                                                                          >  1:265192/1‑150 (MQ=255)               gTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCtt                                                                                                                                        >  2:572850/1‑149 (MQ=255)               gTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTa                                                                                                                                       <  2:1476852/150‑1 (MQ=255)                 ttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAt                                                                                                                                     <  2:84827/150‑1 (MQ=255)                  tACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑g                                                                                                                                   <  1:602086/150‑1 (MQ=255)                   aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCg                                                                                                                                 <  1:309191/151‑1 (MQ=255)                   aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGGGTACAGCTTAAT‑Gc                                                                                                                                  >  1:17810/1‑150 (MQ=255)                    cGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGCGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGCGGCGGGGAGTGGAAAGCCTaaaac                                                                                                                                   >  2:393204/1‑146 (MQ=255)                       ttGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCtttaatcggggg                                                                                                                              >  2:1115468/1‑141 (MQ=255)                       ttGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGa                                                                                                                              <  1:1216155/150‑1 (MQ=255)                        tGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAg                                                                                                                             <  2:296463/150‑1 (MQ=255)                         gggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAgg                                                                                                                            <  2:1073119/150‑1 (MQ=255)                             gcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGata                                                                                                                       <  1:582295/151‑1 (MQ=255)                               gTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAGGGGACAGGCTAAA‑GCggggggggggttt                                                                                                                     >  2:944309/1‑144 (MQ=255)                                tttGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTAAT‑GCGGGGGGCGATAtt                                                                                                                     >  1:231548/1‑150 (MQ=255)                                  tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCg                                                                                                                   <  2:1285996/150‑1 (MQ=255)                                   gAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAAT‑GCGGGAGGCGGTATTcccc                                                                                                                 >  1:485333/1‑151 (MQ=255)                                   gAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGcc                                                                                                                 <  2:232803/151‑1 (MQ=255)                                     aaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGGGGACAGCTTAAT‑GCGGGGGGCGATATTTGccc                                                                                                                >  1:213904/1‑150 (MQ=255)                                       tCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAAT‑GCGGGGGGCGATATTCGCCCTcc                                                                                                             >  1:77790/1‑151 (MQ=255)                                        ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTcc                                                                                                             <  1:1206844/150‑1 (MQ=255)                                        ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCcg                                                                                                            <  1:154203/151‑1 (MQ=255)                                           aCGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGACGGGGAGTGTAAAGCTTTAA‑GCGGGAGGGGGTAtttacc                                                                                                                 >  1:642202/1‑139 (MQ=255)                                            cGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTAAAGCTTTAT‑TCGGGGGGGGGTATTCGCCCTcccc                                                                                                           >  2:1313104/1‑146 (MQ=255)                                             gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAGGGGACCGCCTACT‑TCGGCGGGGGATATTTGCCCCCcccg                                                                                                          >  2:1119896/1‑148 (MQ=255)                                                aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAg                                                                                                     <  2:923393/150‑1 (MQ=255)                                                  cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTTAT‑GCGGGGGGGGGTATTCGCCCCCcgcg                                                                                                          >  2:747830/1‑143 (MQ=255)                                                      cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGc                                                                                              <  1:1401059/151‑1 (MQ=255)                                                       gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCg                                                                                             <  2:1453376/151‑1 (MQ=255)                                                        cAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCATAAT‑GCGGGGGGCGATATTCGCCCTCCCCGTGCCGACGAAGCgg                                                                                            >  1:1324462/1‑151 (MQ=255)                                                        cAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGGTTTAT‑ACGGGCGGCGATATACGCCCTCCCCGGGGCGACGaa                                                                                                >  1:283463/1‑147 (MQ=255)                                                         aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGt                                                                                           <  1:968560/151‑1 (MQ=255)                                                           cTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTTAA‑GCGGGAGGCGGTATTTGCCCTCCGCGTGCCGACGAAGCgg                                                                                            >  1:184594/1‑148 (MQ=255)                                                             gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAg                                                                                        <  1:632339/150‑1 (MQ=255)                                                               ggtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAAT‑TCGGGAGGGGATATTCGCCCTCCGCGGGGAGACGAAGAGGGCAgcg                                                                                      >  1:687688/1‑150 (MQ=255)                                                               ggtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgcgt                                                                                     <  2:686146/151‑1 (MQ=255)                                                                   gATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgcgtgc‑gt                                                                                <  2:27210/151‑1 (MQ=255)                                                                    aTTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑Gtt                                                                               <  1:1135710/151‑1 (MQ=255)                                                                     ttAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAGGGGACAGCTTAAA‑GCGGGGGGGGAAATTTGCCCCCCCCGTGCAGAAGAAGGGGGCAgcgggg                                                                                   >  2:485171/1‑144 (MQ=255)                                                                      tAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑Gttt                                                                              <  1:660111/150‑1 (MQ=255)                                                                      tAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑Gttt                                                                              <  1:1209934/150‑1 (MQ=255)                                                                       aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTc                                                                             <  2:1145406/150‑1 (MQ=255)                                                                         aTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTTAAGCTTAAT‑GCGGGGGGGGATATTTGCCCTCCCCGTGCAGACGAAGCGGGCAGCGGGC‑GGTTCaa                                                                           >  2:640017/1‑150 (MQ=255)                                                                          tCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCCTTAT‑TCGGGGGGCGGTATTCTCCCCCCCCGGGCAGACGAAGAGGTGCGCGTGG‑GGTTCa                                                                            >  2:1037474/1‑148 (MQ=255)                                                                            cGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAGTGGAGAGCTTAAT‑GAGGGGGGCGGTATTTGCCCTCCCCGCGCAGACGGAACGGGCCGCGTGCGGTTTCaagc                                                                         >  2:1011251/1‑148 (MQ=255)                                                                                      cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTAATGCTTAATAGCGGG‑GGGGATATTTTCCCCCCCCGCGGAGGAGAAACAGGCAGGCTGC‑GGTTCAAAGGGCCCGGAtttg                                                             >  1:495172/1‑149 (MQ=255)                                                                                       aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAAT‑TCGGGGGGGGGTATTCGCCCCCCCCGTGCAGACGAAGGGGTCAGCGTGG‑GGTTCCAAGCGGCAGGATagct                                                            >  1:616816/1‑151 (MQ=255)                                                                                         aGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGGGGGGAAAGCTTAAT‑TCGGGGGGGGATTTTTGCCCCCCCCCTGCCGGCGAAGAGGGCAGCGGGC‑GGGTCAAAGCGCCCGGag                                                                >  2:58233/1‑144 (MQ=255)                                                                                            gAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCat                                                        <  2:130410/150‑1 (MQ=255)                                                                                            gAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCCGGGGAGAGGACAGCTTTAA‑GCGGGGGGGGATATTTGCCCTCCCCGTGCAGAAGGAGCGGGCCGCGGGG‑GTTTCAAAACGCCAGGGtttgtgga                                                         >  2:444178/1‑143 (MQ=255)                                                                                              aaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATAtt                                                     <  2:1159553/151‑1 (MQ=255)                                                                                              aaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATAtt                                                     <  2:172158/151‑1 (MQ=255)                                                                                                 cATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGt                                                   <  2:1088366/150‑1 (MQ=255)                                                                                                  aTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAGCTTTAA‑TCGGGGGGGGGTAATTGGCCCCCCCGGGGAGAAGAAAAGGGCACCGGGC‑GGTTCTAAACGCCCCGGTTGCTGCaaaattt                                                   >  2:939631/1‑143 (MQ=255)                                                                                                     gtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGGGTACAGCTTAAA‑GCGGGGGGCGGGATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGCGTGC‑GGTTCAAAGCGCCCGGGTTTCTGCATATTGTCggg                                               >  1:1082061/1‑150 (MQ=255)                                                                                                       gCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCCGGGGAGGGTAAAGCTTTAT‑GCGGGGGGGGGTAATTGCCCCCCGCGCGCAGAAGAAGCGGGCAGCGGGG‑GGTTCAAAACGCCCGGGTTGCTGCCTAtttttgg                                                >  2:678978/1‑142 (MQ=255)                                                                                                         cGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTaa                                          <  2:1532138/151‑1 (MQ=255)                                                                                                          gTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGGTTAAT‑GCGGGGGGGGGTATTTGCCCTCCCCGTGCAGACGAAGCGGGCAGCGGGC‑GTTTCAAAGCGCCCGGGTTGGCGCATATTGTCGGGGGTaa                                          >  1:116559/1‑150 (MQ=255)                                                                                                          gTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAc                                         <  2:43869/151‑1 (MQ=255)                                                                                                            ggCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcg                                        <  1:801836/150‑1 (MQ=255)                                                                                                              cAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTTAT‑GGGGGGGGGGGTATTCGCCCTCCCCGGGCAGACGAAGCGGGCAGCGGGC‑GGTTCAAAACGCCAGGGTTGCTGCATATTTTTggggggaaacgcg                                     >  1:1361031/1‑141 (MQ=255)                                                                                                               aGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAAA‑TCGGGAGGCGGTATTTGCCCCCCCCGGGCAGACGAAACGGGCAGCGGGC‑GGTTTAAAACGCCCGGAGTTCTGCATATTGTTGGGCGTAAaggcgg                                    >  2:483905/1‑151 (MQ=255)                                                                                                                gACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAAT‑TCGGGGGGGGATATTTGCCCCCCCCGGGCAGACGAAGCGGGCAGCGGGC‑GTTTTAAAACGCCAGGGTTGCTGCATATTTTCGGGGGGAAaggcgg                                    >  1:1180981/1‑150 (MQ=255)                                                                                                                 aCGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGAc                                  <  1:439454/151‑1 (MQ=255)                                                                                                                   gTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAAT‑GCGGGGGGGGGTATTTGCCCTCCCCGTGCAGACGAAGCGGGCAGCGTGC‑GGTTTAAAACGCCAGGGTTGCTGCATATTTTCGGGCGTAAcggcgg                                    >  1:36269/1‑147 (MQ=255)                                                                                                                     ggCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACAGCTTAAA‑TCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGGCCGCGGGG‑GGTTCAAAACGCCAGGAGTGCTGCATATTGTCGGGCGGAAccgcggggggg                               >  2:1275238/1‑145 (MQ=255)                                                                                                                     ggCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTaa                              <  2:1390454/151‑1 (MQ=255)                                                                                                                      gCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTAAA‑ACGGGGGGGGGTATTCGCCCTCCGCGGGCAGACGAAAAGGGCAGCGGGG‑GTTTTCAAACGCCCGGGGTTCTGCATATTGTCGGGGGGAACGGGGGGCGGaa                              >  2:665023/1‑150 (MQ=255)                                                                                                                        ttCAACCGCTGGAATTACCGGCGGGGCTGGGGCGAACACAACTTAAT‑GCGGGAGGGAATATTCGCCCCTCGCGCGCAGGAGAAACGGGCCGCTTGC‑GGCTACAAACGCCGGGAATGATGCGTATTTTCGGGAGGAACGGCGGACGAAAGc                            >  1:1209896/1‑150 (MQ=255)                                                                                                                          cAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTa                          <  1:300027/150‑1 (MQ=255)                                                                                                                           aaCCGCTGGAATTACCGGCGGGGCTGGGGAGGGTAAAGCTTTAA‑GCCGGGGGGGGTATTCGCCCTCCCCGTGCAGACGAAGCGGGCCGCGGGC‑GGTTTAAAACGCCAGGAATGCTGCATATTGTTGGGGGGAAAGGCGGGCGGaaagtga                         >  2:1116066/1‑145 (MQ=255)                                                                                                                           aaCCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTTGCCCTCCGCGTGCCGACGAAGCGGGCAGCGGGC‑GGTTTAAAGCGCCAGGGTTGCTGCATATTGTCGGGCGTAACGGCGGGCGGTAGCTaa                         >  1:467453/1‑150 (MQ=255)                                                                                                                            aCCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAg                       <  2:1017139/151‑1 (MQ=255)                                                                                                                             ccGCTGGAATTACCGGCGGGGCTGGGGAGTGTACACCCTAAT‑TCGGGGGGCGATATTCCCCCCCCCCCCGGAGACGCAAAGGGGCGCGTGC‑GGTTCAAAAAGCCAGGCTTGCTGCGTTTTTTTGGGCGCAACGGCGGaactaaacgaaa                        >  1:1053820/1‑138 (MQ=255)                                                                                                                                  ggAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGaaaa                   <  1:515446/149‑1 (MQ=255)                                                                                                                                   gAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCt                <  1:485190/151‑1 (MQ=255)                                                                                                                                    aaTTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTg               <  1:781647/151‑1 (MQ=255)                                                                                                                                     aTTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTgg              <  2:1406335/151‑1 (MQ=255)                                                                                                                                             cggGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGc       >  2:1443238/1‑150 (MQ=255)                                                                                                                                             cggGGATGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCt      >  2:864679/1‑151 (MQ=255)                                                                                                                                              ggggCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTg     >  1:70023/1‑151 (MQ=255)                                                                                                                                                 gCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGc  <  2:77787/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                   AGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAT‑GCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGC  >  NC_000913/213191‑213485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑