Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,555,1090TA44.9% 139.5 / 43.6 99N178K (AAT→AAA) uxuRfructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (12/42);  new base A (44/0);  total (57/42)
Fisher's exact test for biased strand distribution p-value = 3.92e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTA  >  NC_000913/4554962‑4555257
                                                                                                                                                    |                                                                                                                                                      
gCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATc                                                                                                                                                       <  1:407890/149‑1 (MQ=255)
  aaCTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAAAccca                                                                                                                                                    >  1:1019152/1‑148 (MQ=255)
   aCTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAAtg                                                                                                                                                  <  1:1284856/151‑1 (MQ=255)
   aCTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAAccca                                                                                                                                                    >  1:335299/1‑147 (MQ=255)
      ggAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTgg                                                                                                                                               <  2:549469/151‑1 (MQ=255)
      ggAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTgg                                                                                                                                               <  1:858405/151‑1 (MQ=255)
           agCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCa                                                                                                                                          <  1:973892/151‑1 (MQ=255)
            gCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAACAAACCAATGTGGGTcc                                                                                                                                           >  1:510412/1‑149 (MQ=255)
            gCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAg                                                                                                                                         <  1:1052288/151‑1 (MQ=255)
            gCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAg                                                                                                                                         <  2:1375577/151‑1 (MQ=255)
              gTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGtt                                                                                                                                       <  1:312462/151‑1 (MQ=255)
                gAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGc                                                                                                                                     <  2:671687/151‑1 (MQ=255)
                 aaCTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGca                                                                                                                                    <  2:271406/151‑1 (MQ=255)
                  aCTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAAACAATGTGGGTCCCGTTGca                                                                                                                                    >  1:36579/1‑150 (MQ=255)
                    tGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAAACAATGTGGGTCCAGTTGcac                                                                                                                                   >  1:332656/1‑149 (MQ=255)
                      gCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAAACCAA‑GTGGGGCCCGTTGCTCAAcc                                                                                                                               >  2:967042/1‑151 (MQ=255)
                        ttCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAAAAATCCAAAGGGGGGTCAGTTGCACAggc                                                                                                                               >  2:1190872/1‑147 (MQ=255)
                         tCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCAt                                                                                                                             <  2:1081665/150‑1 (MQ=255)
                          ccAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCt                                                                                                                           <  1:808486/151‑1 (MQ=255)
                          ccAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAAATCAATGTGGATGCCGCTGGACAGCCCTc                                                                                                                            >  1:936727/1‑150 (MQ=255)
                              tGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGAt                                                                                                                       >  1:273820/1‑151 (MQ=255)
                                 gcCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCAATGTGGGTCCCGTTGCACAGCCCTCTgggt                                                                                                                       >  1:121749/1‑146 (MQ=255)
                                  ccGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAAAAATCCAATGTGGGTCCCGTTGCACAGCCATCTGGAt                                                                                                                       >  1:238241/1‑147 (MQ=255)
                                  ccGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGGTCCAGTTGCACAGCCCTCTGGGTggc                                                                                                                    >  1:470597/1‑148 (MQ=255)
                                   cGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCCATGTGGATCCCGTTGCACAGCCATCTgg                                                                                                                         >  1:769526/1‑144 (MQ=255)
                                      gcagccTCGAAAGCGGTTCCATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCt                                                                                                               <  1:833332/151‑1 (MQ=255)
                                       cagcagCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAAACCAATGTGGGTCCCGTTGCACAGCCCTCTGGGTGGCAGcc                                                                                                                >  2:1350828/1‑149 (MQ=255)
                                        agcagcGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAAACCAAAGTGGGTCCAGTTGCACAGCCACCTGGGTGGAAGCCTGt                                                                                                             >  2:787166/1‑151 (MQ=255)
                                        agcagcGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCAAAGTGGGTCCCGTTGCACAACCATCTGGGTGACAACCTg                                                                                                              >  1:1042299/1‑150 (MQ=255)
                                             cGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAAACCAATGTGGATCCAGTTGCACAGCCATCTGGGTGACAGCCTGTATCg                                                                                                         >  1:53832/1‑150 (MQ=255)
                                              gAAAGCGGTGACATGCAGTTCCATCTTGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAAAAAAACAAAGTGGGTCCAGCTGTACAGCCCTCTTGGTGACAGCCTGTAttg                                                                                                         >  2:1121917/1‑147 (MQ=255)
                                               aaaGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCAATGTGGGTCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGc                                                                                                        >  1:520329/1‑149 (MQ=255)
                                               aaaGCGGAGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑aa                                                                                                     <  1:823650/151‑1 (MQ=255)
                                                   cGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAaga‑g                                                                                                <  1:817864/151‑1 (MQ=255)
                                                    ggTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑Gt                                                                                               <  1:1214626/151‑1 (MQ=255)
                                                     gTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAACAATCCAATGTGGGGCCAATTGCACCGCCCACTGGGTGAAAGCCCGGATCGC‑TAaga‑g                                                                                                >  1:1181296/1‑149 (MQ=255)
                                                         cATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCCGTGGC‑GGGAAAACAATCCAATGTGGGTCCAGTTGCTCAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGtt                                                                                           <  2:1100739/150‑1 (MQ=255)
                                                           tGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAAACAATGTGGGTCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑Gtgggtggg                                                                                        >  2:1477037/1‑151 (MQ=255)
                                                             cAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCg                                                                                      <  1:1345925/151‑1 (MQ=255)
                                                              aGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCCATGTGGGTCCCGTTTGCCAGCCCTCTGGGTGGCAGCCCGTTTTCC‑AAAGAGGGGGTTgggg                                                                                       >  2:575047/1‑149 (MQ=255)
                                                                 tCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAACAAAACAATGTGGGTTCAGTTGCACAACCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GAGGTTGGGCg                                                                                      >  1:977158/1‑147 (MQ=255)
                                                                 tCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAAAAAAACAAAGTGGGTCCAGTTGCCCAGCCATCCTGATGAAAGCCTGGATCGC‑AAAGA‑GGGGGTggggggac                                                                                   >  2:1220481/1‑145 (MQ=255)
                                                                 tCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGGACCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑CAAGA‑GAGGGTgggggctc                                                                                   >  1:618763/1‑145 (MQ=255)
                                                                 tCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCAATGTGGGTCCAGTTGCACAGCCCTCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGGTGGGCGGTc                                                                                   >  1:93249/1‑150 (MQ=255)
                                                                  ccATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATcac                                                                                 <  2:327252/151‑1 (MQ=255)
                                                                   cATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAACAA‑CCCACGTGGGATCAGTTGCACAACCCCGCGGGTGAAAGCCTGTGTCGC‑AAAGA‑GGGGGTggg                                                                                        >  2:373757/1‑143 (MQ=255)
                                                                   cATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATcaca                                                                                <  2:711916/151‑1 (MQ=255)
                                                                   cATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATcaca                                                                                <  1:210850/151‑1 (MQ=255)
                                                                       tcGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAAAAAACCAAAGCGGAGCCCGGTGCACAGCCCTCTGGGTGACAACCTGGATTGC‑AAAGA‑GGGGGTGGGCggacacca                                                                               >  2:1161920/1‑141 (MQ=255)
                                                                       tcGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAAACAAAGGGGGTCCCGGTGGACAGCCCTCTTGGTGAAAGCCTGTATCGC‑CAAGA‑GGGGGTGGGGGAGCACaaa                                                                              >  2:335916/1‑149 (MQ=255)
                                                                         gCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGGTCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑CAAGA‑GGGGGTGGGCGATCACaaa                                                                              >  1:1461674/1‑147 (MQ=255)
                                                                         gCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCCGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGGTGGGCGATCACaaa                                                                              >  1:1020900/1‑147 (MQ=255)
                                                                         gCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGa                                                                          <  2:1492241/151‑1 (MQ=255)
                                                                         gCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCAATGTGGGTCCAGTTGCACAGCCCTCTGGGTGACAGCCCGTATCGC‑AAAGA‑GTGGGTGGGCGGTCCCaaa                                                                              >  1:481018/1‑147 (MQ=255)
                                                                           tATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAAACCCATGTGGGTCCAGTTGCCCAGCCCCCTGGGTGACAGCCCGTATCGC‑AAAGA‑GTGGTTGGGGGCTCAaaaaaaaaac                                                                        >  1:402923/1‑145 (MQ=255)
                                                                           tATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAAACCCATGTGGATCCAGGTGCACAGCCATCTGGATGACAGCCCGTATTGC‑CAAAA‑GGGGGTGGGGGGTCACaaa                                                                              >  1:359681/1‑145 (MQ=255)
                                                                              tGCCGAAGCAAGGCATAACAGCATGCTGGCGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAAGCCATGTGGGTACCGTTGGGCGGCCCTGTTGGTGGAAGGCAGTATCGC‑CAAGC‑GTGGGGGGGGGATCACAAAAAAATCCt                                                                      >  2:932627/1‑150 (MQ=255)
                                                                               gCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTcg                                                                    <  2:890779/151‑1 (MQ=255)
                                                                               gCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAAAACAAAGTGGAACCAGTTGCCCCGCCATCTGGGTGACAGCCTGTATCTC‑AAAGA‑GGGGTTGGGCGATCACAAACAGATcc                                                                       >  1:478109/1‑148 (MQ=255)
                                                                                  gAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCCAAGTGGATCCCGGTGCACAGCCATCTGGATGACAGCCTGTATCGC‑CAAGA‑GGGGGTGGGGGGTCACAAACAGATCCTcgcc                                                                  >  1:809209/1‑150 (MQ=255)
                                                                                  gAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCAATGTGGGTCCAGTTGCCCAGCCCTCTGGATGACAGCCCGTATCGC‑CAAAA‑GTGGGTGGGCGATCACAAACAGATCCTcgcc                                                                  >  1:563981/1‑150 (MQ=255)
                                                                                  gAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCAACATGGATCAAGATGCACAGACACCTGGGTGACAGCCCGTATAGCAAAAGA‑GGGGTTGGGGGAGAACaacaaaatctcgcc                                                                   >  2:280458/1‑138 (MQ=255)
                                                                                      cAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGtt                                                             <  2:171720/151‑1 (MQ=255)
                                                                                        aCGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTaa                                                           <  2:151883/151‑1 (MQ=255)
                                                                                        aCGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTaa                                                           <  1:725576/151‑1 (MQ=255)
                                                                                         cGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAAAAAACCAATGTGGGTCCAGTTGCACCGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GGGGGTGGGCGGTCACAAACAAAACCTCGCCGCGTTaa                                                           >  1:1325370/1‑150 (MQ=255)
                                                                                           cATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCa                                                        <  2:1019108/151‑1 (MQ=255)
                                                                                            aTAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAAAAAACCCATGTGGGTCCCGGTGCACAGCCCACTGGGTGACAGCCCGTATTGC‑AAAGA‑GGGGGTGGGGGATCCCAAACAAAACCTCCCCCCGTTaa                                                           >  2:1495462/1‑147 (MQ=255)
                                                                                                cAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCaaaaaa                                                   <  2:1146681/151‑1 (MQ=255)
                                                                                                 aGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAACAAACAAATGTGGGACCCGGTGCCCCGCCATCCGGGTGACAGCCCGGATCGC‑CAAAA‑GGGGGTGGGCGAACACCAAAAAAAACCCCCCGCGCTTATCaaaaaa                                                   >  2:391646/1‑150 (MQ=255)
                                                                                                 aGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCCATGTGGGTCCCGGTTCACAGCCATCTGGGTGACAGCCTGTAACGC‑CAAGA‑GTGGTTGGGCGATCACAAAAAGATCCTCCCCGCGGTAATCaaaaaa                                                   >  2:1334605/1‑150 (MQ=255)
                                                                                                 aGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAAACAATGTGGGTCCAGTTGCACAACCATCTGGATGACAGCCCGTATCGC‑AAAGA‑GGGGTTGGGGGATCACAAACAGATCCTCGCCGCGTTAAATaaaaaa                                                   >  1:176350/1‑150 (MQ=255)
                                                                                                    aTGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCAATGTGGGTTCCGGTGGACAGCCCTCTGGATGACAGCCTGTATCGC‑AAAGA‑GGGGGTGGGGGATCACAAAAAGATCCTCGCCCCGGTAATCaaaaaaaa                                                 >  1:542105/1‑147 (MQ=255)
                                                                                                      gCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAACAAACCAATGTGGGAACAGTTGCACAGTCATCTTGGTGGAAACCTCTATCGC‑CAAGA‑GAGGTTGGGGGGGCACAAACAGAAACCCCCCGCGGTAATAAAAAAAGATGcc                                             >  2:668306/1‑151 (MQ=255)
                                                                                                       cTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCAATGTGGGTCCAGTTGCCCAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GGGGGTGGGCGATCACAAAAAGATCCTCGCCGCGTTAATCAAAAAAAAAGccc                                            >  1:741124/1‑151 (MQ=255)
                                                                                                        tggtggAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAAAAAACCAATGTGGGTCCAGGTGCACAGCCACCCGGATGAAAGCCTGTATCGC‑CAAGA‑GTGGGTGGGGGATCACCAAAAAATCCTCGCCCCGCTAATTAAAAAAAATGccc                                            >  1:1142953/1‑150 (MQ=255)
                                                                                                          gtggAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAg                                         <  2:810247/151‑1 (MQ=255)
                                                                                                          gtggAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCCATGTGGGTCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GGGGGTGGGGGGTCACAAACAAATCCCCCCCCCGTTAATCAAAAAAGaaggcc                                            >  1:116053/1‑143 (MQ=255)
                                                                                                            ggAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAgc                                        <  2:27569/150‑1 (MQ=255)
                                                                                                            ggAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCAATGTGGGTCCAGTTGCACAACCATCCGGATGACAGCCCGTATCGC‑AAAAA‑GGGGGTGGGCGGTCACAAACAAATCCCCGCCGCGGTAATAAAAAAAGAttaccggg                                         >  2:418687/1‑142 (MQ=255)
                                                                                                            ggAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCAAATTGGGTCCAGTTGCCCAGCCATCTGGATGACAACCCGTATCGC‑CAAAA‑GGGGGTGGGGGGTCACAAACAGAACCTCGCCGCGGTAATCAAAAAAAATGacc                                            >  1:1330973/1‑146 (MQ=255)
                                                                                                             gAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAgcgg                                      <  1:905333/151‑1 (MQ=255)
                                                                                                                cTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAAAAAACCCATGTGGGTCCCGTTGCACAACCCTCCGGTGGACAACCCGTTTTCC‑AAAGA‑GAGGCTGGGCGGACAAAAAAAGAATCCCCCCGCGCTAATCAAAAAAAAAGGCCGAgcgg                                      >  1:704663/1‑148 (MQ=255)
                                                                                                                 tGTTCCGTCAGTCCTGGCAGTGGC‑GGGCAAAAAAACCAATATGGGTCCCGTTGTACAGCCATCTCGGTGAAAAACTGTATCGC‑CAAAA‑GGGGGTGGGGGATCACAAACAAATCCTCGCCGCGGTAATAAAAAAAAAAGCCCCAgcggcg                                    >  1:87497/1‑149 (MQ=255)
                                                                                                                  gTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGaa                                  <  1:772911/150‑1 (MQ=255)
                                                                                                                     ccGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCt                               <  1:859940/150‑1 (MQ=255)
                                                                                                                      cGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGGGCCAGGTGCACAGCCATCTGGGTGACAGCCTGTATCGC‑AAAGA‑GGGGGTGGGCGGTCACAAAAAGATCCTCGCCGCGTTAATAAAAAAAAAAGCCCGAGCGGGGAAGCTgg                             >  2:1357279/1‑151 (MQ=255)
                                                                                                                       gTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGc                            <  2:1072361/151‑1 (MQ=255)
                                                                                                                          aGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAAt                         <  2:1200892/151‑1 (MQ=255)
                                                                                                                             ccTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAAtgt                       <  2:1140253/150‑1 (MQ=255)
                                                                                                                                ggCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGgca                   <  1:1494467/151‑1 (MQ=255)
                                                                                                                                      ggC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATc              <  1:1482178/150‑1 (MQ=255)
                                                                                                                                        c‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTgg           >  1:1162960/1‑151 (MQ=255)
                                                                                                                                        c‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTgg           <  1:1516523/151‑1 (MQ=255)
                                                                                                                                           ggAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGaa         <  1:1190926/151‑1 (MQ=255)
                                                                                                                                           ggAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGaa         <  1:481969/151‑1 (MQ=255)
                                                                                                                                           ggAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGa          >  2:1051616/1‑150 (MQ=255)
                                                                                                                                            gAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGaaa        <  2:1101034/151‑1 (MQ=255)
                                                                                                                                               aaCAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACg     >  2:1492138/1‑151 (MQ=255)
                                                                                                                                                    tCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTa  >  2:129479/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
GCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGC‑AAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTA  >  NC_000913/4554962‑4555257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑