Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8900CA56.6% 14.7 / 67.4 53L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (17/6);  new base A (0/30);  total (17/36)
Fisher's exact test for biased strand distribution p-value = 3.12e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-07

GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGAC  >  NC_000913/357742‑358039
                                                                                                                                                        |                                                                                                                                                       
gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaac                                                                                                                                                         <  1:1232236/149‑1 (MQ=255)
 aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaa                                                                                                                                                     <  1:1301364/151‑1 (MQ=255)
   aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaaa                                                                                                                                                    <  1:1205811/150‑1 (MQ=255)
   aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc                                                                                                                                                   >  1:1303713/1‑151 (MQ=255)
      cGTAGCGCATCAGGCAATTCCACCCGCTGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAcc                                                                                                                                                  <  2:1219159/149‑1 (MQ=255)
            gcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtc                                                                                                                                           >  2:554059/1‑150 (MQ=255)
               tCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCTCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctctc                                                                                                                                       >  1:1057746/1‑151 (MQ=255)
                 aGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTcc                                                                                                                                      >  1:1185853/1‑150 (MQ=255)
                       ttCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCCCTCCAGGAGTg                                                                                                                               >  2:674692/1‑151 (MQ=255)
                         ccACCCGCAGCCTTGTGTCGGCGGCTCCCGTGTTTCTTTCCCGCCAACACCCGCGCTTTTACCAACCGCCTGGCGAAAAAATCCCTCCGGGGGGGTTGTCTGCCAGTTTTTTTCCCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGc                                                                                                                             <  1:1324225/151‑1 (MQ=255)
                          cAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCg                                                                                                                           >  2:715539/1‑151 (MQ=255)
                             ccGCAGCCTTGTCTCGGCGCCTCCTGTTATTTATTCCCGCAAACACCCGCGCATTAATCCAACGCCTTGTGAAAAATGTCTTCCGCGGCGCCTGTCTGCAATTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCagtag                                                                                                                                 <  2:171586/143‑1 (MQ=255)
                              cccagccatgtgCCGGCGGCTGCCGTGATCCATCCCCCCCAACACCCGCGCTTTTACCCACCCCGATGTAAAAAAGTTCGTCGGCGGCGCCTGCCGCCCCGCTATTTTCCCCGCCAAACAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                                         <  2:1456042/141‑1 (MQ=255)
                              cGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                        >  1:547115/1‑150 (MQ=255)
                                  gcccTGTGTCAGCGCCTACCGTTTTTCTTTCCCGCCAACACCCGCGCTTTTACCCACCGCCATGTAAAAAAATGCCTTCGCGGCGGCTTTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAag                                                                                                                    <  1:657477/148‑1 (MQ=255)
                                     cTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                        >  1:585558/1‑143 (MQ=255)
                                        tttgggCGTCTACCGTGATTCTTCCCCCCCAACACCCGCGCTTTTATCCAGCCCCATGGGAAAAAGTCCCTCCGCGGCGGCTGTGTCCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGaaa                                                                                                               <  1:664317/144‑1 (MQ=255)
                                        tgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggggagggggaa                                                                                                              >  2:692297/1‑140 (MQ=255)
                                             gcgcCTCCCGTGTTTCATTCCCCCCACCACCCGCGCTTTTCCCCACCCCTTTGTAAAAAAATCCTTCGGCGGCGGCTGTCTCCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAg                                                                                                            <  2:98300/147‑1 (MQ=255)
                                               ggCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGaaaaaaattt                                                                                                        >  1:858129/1‑142 (MQ=255)
                                               ggCTACCGTAATTCTTTCCCGCCAACACCCGGGCTTTTACCCACCCCCCTGTAAAAAAACGCCTGCGCGGGGGCTGCCTCCCAGTTTTTTTCCCGCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAt                                                                                                              <  1:15446/143‑1 (MQ=255)
                                               gcctcccGTGTTTCTTTCCCGCCACCACCCCCGCTTTTACCAACCGCCAGGTCAAAAAAGCCTTCCGCGGCGGTTGCCTGCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGaa                                                                                                                <  2:230456/136‑1 (MQ=255)
                                               gcctcccGTGATTAATTCCCGCAACCACCCGCGCTTTTACCCACCCCCATGTGAAAAAAGCCCTTCGCGGCGGCTTTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCt                                                                                                          <  1:949156/142‑1 (MQ=255)
                                                gCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGgaaaaaattt                                                                                                        >  1:181921/1‑141 (MQ=255)
                                                 cccccGTGTTTCTTTCCCGCCAACACCCCCGATTTTACCCACCGCTATGTCAAAAAATCCTTCCGCGGCGGCTTTCTGCCAGTTTTTTTCCCGCCACAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAg                                                                                                     <  2:1217260/147‑1 (MQ=255)
                                                 cTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAGAAGaaaaaaactt                                                                                                        >  2:1323704/1‑140 (MQ=25)
                                                   ccggttaTTAATTCCCGCCAACACCCCCCCATTTACCCACCCCATTGTGAAAAAATCCCTCGGCGGCGGCTTTCGGCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGccc                                                                                                  <  2:1346563/145‑1 (MQ=255)
                                                   aCCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGAAAAAACTTTAAAccc                                                                                                  >  1:598663/1‑151 (MQ=255)
                                                     cggtttttaTTTCCCCCAACAACCCGCGCATTTACCCACCCCCTTGTAAAAAATTCCCTCCGGGGCGGTTGTCGGCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCtt                                                                                                         <  2:1254825/135‑1 (MQ=255)
                                                              ttccccccAAAACCCGCCCTTTTACCCACCCCAATGTAAAAAAATCCCTGCGCGGGGGCTGTCTGCCAGTTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgt                                                                                           <  1:592878/141‑1 (MQ=255)
                                                              tccccGCCACCACCCGCTTTTTTACCCCACGCCATGTAAAAAAATCCTTCCGCGGGGGCTGTCTGCCAGTTTTTTTCCCGCCACACAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGACCGGCAtgttg                                                                                         <  2:608206/146‑1 (MQ=255)
                                                                 cccccAACACCCGCGCTTTTACCCACCGCCTTGTGAAAAATTGCTTTGGGGGCGGCTTTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTtgtgt                                                                                      <  1:252997/146‑1 (MQ=255)
                                                                  ccccaaccacccccgcTTTTACCCACCCCCATGGGAAAAATTCCCTGCGGGGCGGTTGTCGCCAATTTTTTTTCCCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgt                                                                                           <  2:954952/131‑1 (MQ=255)
                                                                      aacaccCGCGCATTTACCCACCCCCTTGTGAAAAAATTCCTTCGGGGCGGCTTTCTGCCATTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAAt                                                                                <  1:1368108/150‑1 (MQ=255)
                                                                            ccgcacttttccccACCGCCATGTAAAAAAGTGCATGGGCGGGGGCGGTCGGCGGTTTTTTTGCCCGCCACAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATc                                                                               <  2:688549/134‑1 (MQ=255)
                                                                              gcgcATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAAAAAAAAAAACTTTAACCCCTCCTGTTTTGTTGGAATccc                                                                             >  2:1309730/1‑144 (MQ=25)
                                                                                      aTCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGAAAAAAATTTTAACCCCTCCATTTGTGTTGCGAACGCTGCTggggaaaa                                                                 >  2:774083/1‑144 (MQ=25)
                                                                                        ccaccgccagggaaaaaaaTGCCTGCGCGGCGCCTGTCTCCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAc                                                                   <  2:1276166/132‑1 (MQ=255)
                                                                                            ccccttgtaaaaaaaTGCTTCCGCGGCGGTTGCCGGCCAGTTTTTTTCCCCCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtg                                                               <  2:852803/136‑1 (MQ=255)
                                                                                              ccagggaaaaaaaTGCCTCCGGGGCGGTTTTCGGCCAGTTTTTTTCCCCCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGa                                                           <  2:751548/140‑1 (MQ=255)
                                                                                                  gtcaaaaaaTCCCTGCGGGGCGGTTGTCTCCCATTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAgg                                                       <  1:62683/144‑1 (MQ=255)
                                                                                                  gtaaaaaaaGGCCTCCGCGGGGCTTTTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAgg                                                       <  2:75230/144‑1 (MQ=255)
                                                                                                   taaaaaaaTCCTTCCGCGGCGCTTGTCTGCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAggg                                                      <  2:476208/145‑1 (MQ=255)
                                                                                                   taaaaaaaTCCCTCCGCGGCGGCTTTCTCCCATTTTTTTTTCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATAGCTGCTGGTAACAATGTGGAAAGGGa                                                     <  1:97284/146‑1 (MQ=255)
                                                                                                   aacAAAAATGCCTCCGCGGCGGTTGTCTGCCGGTTATTTTCCCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAg                                                   <  2:1143154/149‑1 (MQ=255)
                                                                                                        aaaTGCCTGCGGGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcgg                                              <  2:588244/151‑1 (MQ=255)
                                                                                                         aaTGCCTTCGGGGGCGCCTTTCTCCCGTCTTTTTTCCCCCCAAACAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcgg                                              <  2:1513976/151‑1 (MQ=255)
                                                                                                              cTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAAATTTTAAACCCCCCTGTTTTGTGGGGATTGCTGCTGGGAACAAAGGGGGAAGGGGAgggggggg                                            >  2:1343207/1‑140 (MQ=255)
                                                                                                                      ggcggcTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTc                                   <  1:437978/150‑1 (MQ=255)
                                                                                                                      ggcggcTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGACGAGGAAAAGAATATTACCCGACATATTGTGTGGGGCTCGGCGCTGGGGACAATATTGAAAAGGGAGGGCGGGGAGTCAGc                                     >  2:166503/1‑148 (MQ=255)
                                                                                                                                    atcttttttcCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTaa                     <  1:190109/145‑1 (MQ=255)
                                                                                                                                       ttttttcCCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg                  <  2:165898/148‑1 (MQ=255)
                                                                                                                                            ttcccgccgAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGtt              <  1:1205206/147‑1 (MQ=255)
                                                                                                                                                cgccgaAACAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCt         <  1:1103636/151‑1 (MQ=255)
                                                                                                                                                       acaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGac  <  1:585739/151‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                       
GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGAC  >  NC_000913/357742‑358039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: