Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,195	G→A	A298V (GCA→GTA)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,195	0	G	A	100.0%	371.5 / NA	98	A298V (GCA→GTA)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (56/42);  total (56/42)

TTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAA  >  NC_000913/3400050‑3400342                                                                                                                                                  |                                                                                                                                                    ttCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGcc                                                                                                                                                 >  2:520034/1‑150 (MQ=255)  tCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGcc                                                                                                                                                 <  1:41585/149‑1 (MQ=255)   cGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGccacc                                                                                                                                              >  1:1376291/1‑151 (MQ=255)   cGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGccacc                                                                                                                                              >  1:264662/1‑151 (MQ=255)      tGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCaccacc                                                                                                                                           >  1:529559/1‑151 (MQ=255)            ggTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTg                                                                                                                                       >  1:644803/1‑149 (MQ=255)             gTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGc                                                                                                                                    <  1:855863/151‑1 (MQ=255)             gTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGc                                                                                                                                    <  2:1285430/151‑1 (MQ=255)               cgccgcCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCAc                                                                                                                                  <  2:732391/151‑1 (MQ=255)                gccgccGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCAcc                                                                                                                                 <  1:813605/151‑1 (MQ=255)                  cgccgTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCAt                                                                                                                               >  1:1005253/1‑151 (MQ=255)                   gccgTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATg                                                                                                                              >  1:1001335/1‑151 (MQ=255)                   gccgTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATg                                                                                                                              >  2:611075/1‑151 (MQ=255)                    ccgTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGc                                                                                                                             <  2:1029424/151‑1 (MQ=255)                     cgTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGcc                                                                                                                            >  2:1503277/1‑151 (MQ=255)                     cgTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGcc                                                                                                                            >  2:1451805/1‑151 (MQ=255)                      gTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCcg                                                                                                                           >  2:1039823/1‑151 (MQ=255)                       tGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCcgc                                                                                                                          >  2:600952/1‑151 (MQ=255)                        gCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCcgcg                                                                                                                         >  1:5277/1‑151 (MQ=255)                         cATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCgcgc                                                                                                                        >  2:359485/1‑151 (MQ=255)                            gTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTc                                                                                                                      <  1:547851/150‑1 (MQ=255)                            gTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCg                                                                                                                     >  2:1076202/1‑151 (MQ=255)                              cGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCgg                                                                                                                    <  1:389998/150‑1 (MQ=255)                                aTCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCgg                                                                                                                    >  2:255642/1‑148 (MQ=255)                                 tCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCgg                                                                                                                    >  1:443881/1‑147 (MQ=255)                                  cATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATg                                                                                                               >  2:343188/1‑151 (MQ=255)                                  cATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATg                                                                                                               >  2:151177/1‑151 (MQ=255)                                   aTTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGt                                                                                                              >  2:245847/1‑151 (MQ=255)                                     ttCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTc                                                                                                             <  1:874625/150‑1 (MQ=255)                                     ttCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTc                                                                                                             <  1:854712/150‑1 (MQ=255)                                      tCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCgg                                                                                                           >  1:1444772/1‑151 (MQ=255)                                       ccAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGa                                                                                                          <  2:635879/151‑1 (MQ=255)                                        cAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGaa                                                                                                         <  2:918743/151‑1 (MQ=255)                                            gcTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGcc                                                                                                      >  2:55045/1‑150 (MQ=255)                                            gcTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCa                                                                                                     <  1:1081466/151‑1 (MQ=255)                                             cTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGcc                                                                                                      >  2:967690/1‑149 (MQ=255)                                              tttGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACGACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGt                                                                                                   >  1:1027007/1‑151 (MQ=255)                                               ttGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGtt                                                                                                  >  1:1458128/1‑151 (MQ=255)                                                  ccgccACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTGCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGACAGTTCcg                                                                                               <  2:751903/151‑1 (MQ=255)                                                    gccACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTcc                                                                                                <  1:270714/148‑1 (MQ=255)                                                    gccACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcggc                                                                                             >  1:976516/1‑151 (MQ=255)                                                    gccACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcggc                                                                                             >  2:530268/1‑151 (MQ=255)                                                    gccACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcgac                                                                                             <  1:842863/151‑3 (MQ=255)                                                        ccGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGc                                                                                         <  2:1227107/151‑1 (MQ=255)                                                        ccGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGc                                                                                         >  1:942639/1‑151 (MQ=255)                                                           cgcgcCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACt                                                                                      <  1:372808/151‑1 (MQ=255)                                                             cgcCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGt                                                                                    <  2:118376/151‑1 (MQ=255)                                                                             gggTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCAtt                                                                     >  2:484383/1‑150 (MQ=255)                                                                             gggTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCAtt                                                                     >  1:860998/1‑150 (MQ=255)                                                                              ggTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAc                                                                   <  1:309787/151‑1 (MQ=255)                                                                              ggTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAc                                                                   <  1:310694/151‑1 (MQ=255)                                                                               gTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAcc                                                                  <  1:1198080/151‑1 (MQ=255)                                                                               gTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAcc                                                                  >  1:1102003/1‑151 (MQ=255)                                                                               gTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAcc                                                                  <  1:475997/151‑1 (MQ=255)                                                                                 cTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAcc                                                                  >  2:61108/1‑149 (MQ=255)                                                                                   tCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACcgcg                                                               <  2:860779/150‑1 (MQ=255)                                                                                   tCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACcgc                                                                <  2:1131090/149‑1 (MQ=255)                                                                                   tCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACcg                                                                 <  1:1094939/148‑1 (MQ=255)                                                                                     aGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTc                                                            >  2:1410740/1‑151 (MQ=255)                                                                                      gCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCt                                                             >  1:61584/1‑149 (MQ=255)                                                                                       caacaaCGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTcac                                                          >  2:545325/1‑151 (MQ=255)                                                                                       caacaaCGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTcac                                                          >  1:1500861/1‑151 (MQ=255)                                                                                          caacGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAAt                                                       >  1:1069936/1‑151 (MQ=255)                                                                                           aacGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCTGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAAt                                                       <  1:490706/150‑1 (MQ=255)                                                                                           aacGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAAt                                                       >  2:845964/1‑150 (MQ=255)                                                                                           aacGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATa                                                      <  2:318144/151‑1 (MQ=255)                                                                                              gACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATAcc                                                    >  1:1460873/1‑150 (MQ=255)                                                                                              gACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCg                                                   >  1:1388890/1‑151 (MQ=255)                                                                                                 tGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCgg                                                  >  1:15032/1‑149 (MQ=255)                                                                                                  ggAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGt                                                 <  1:494779/149‑1 (MQ=255)                                                                                                    aaTGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGc                                             >  2:1472550/1‑151 (MQ=255)                                                                                                    aaTGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGc                                             <  1:432064/151‑1 (MQ=255)                                                                                                    aaTGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGc                                             >  2:1504297/1‑151 (MQ=255)                                                                                                     aTGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCg                                            <  2:1223303/151‑1 (MQ=255)                                                                                                      tGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCgg                                           >  1:1033913/1‑151 (MQ=255)                                                                                                        ccGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGtt                                         <  1:1316876/151‑1 (MQ=255)                                                                                                              tcttcCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGc                                    <  2:1195937/150‑1 (MQ=255)                                                                                                                ttcCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGt                                 >  1:142392/1‑151 (MQ=255)                                                                                                                 tcCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTg                                >  2:415985/1‑151 (MQ=255)                                                                                                                  ccATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGc                               >  1:637327/1‑151 (MQ=255)                                                                                                                   cATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCt                              >  2:509032/1‑151 (MQ=255)                                                                                                                   cATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCt                              >  1:66609/1‑151 (MQ=255)                                                                                                                    aTTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCtt                             >  1:1081886/1‑151 (MQ=255)                                                                                                                           aaCGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGAt                      <  2:798267/151‑1 (MQ=255)                                                                                                                            aCGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGCGCACCATGCCGCGCTCGGAGATGTCGGCAGCCAGTTCCGGCGGGCACTGTTCCCGTGCAACCATTACCGCGCTCACAATACCGGGCAGCGGTTCCTGCAGTGCTTCGAGGact                     >  2:852593/1‑149 (MQ=255)                                                                                                                             cGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGCGGAttt                    <  2:328356/151‑1 (MQ=255)                                                                                                                             cGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGAttt                    >  2:1435670/1‑151 (MQ=255)                                                                                                                                  aaGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCAtt                >  2:298925/1‑150 (MQ=255)                                                                                                                                   aGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTTCCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTgg              <  2:882574/151‑1 (MQ=255)                                                                                                                                    ggTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGa             <  1:352693/151‑1 (MQ=255)                                                                                                                                     gTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTgg              >  2:208297/1‑149 (MQ=255)                                                                                                                                     gTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAg            <  2:13129/151‑1 (MQ=255)                                                                                                                                       tACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGtt          >  1:402372/1‑151 (MQ=255)                                                                                                                                       tACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGt           <  1:436294/150‑1 (MQ=255)                                                                                                                                         cGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCa        <  2:1218255/151‑1 (MQ=255)                                                                                                                                            agcagTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAggg     <  2:942599/151‑1 (MQ=255)                                                                                                                                               agTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTaa  <  1:948397/151‑1 (MQ=255)                                                                                                                                               agTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTa   >  1:1252155/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAA  >  NC_000913/3400050‑3400342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑