Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,326,4500TG43.4% 194.5 / 23.8 107G114G (GGT→GGG) atoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (10/50);  new base G (46/0);  total (57/50)
Fisher's exact test for biased strand distribution p-value = 2.40e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

GCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGAAAAC  >  NC_000913/2326306‑2326600
                                                                                                                                                |                                                                                                                                                        
gcgTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATgg                                                                                                                                                      >  2:1062433/1‑149 (MQ=255)
 cgTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaaa                                                                                                                                                   <  2:1130521/151‑1 (MQ=255)
   tCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGgaaa                                                                                                                                                  >  2:511532/1‑150 (MQ=255)
    cAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTTTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaaa                                                                                                                                                   <  2:991044/148‑1 (MQ=255)
    cAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaaaa                                                                                                                                                  <  2:1146520/149‑1 (MQ=255)
    cAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAat                                                                                                                                                 <  1:1345033/150‑1 (MQ=255)
    cAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAat                                                                                                                                                 <  1:810964/150‑1 (MQ=255)
    cAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAat                                                                                                                                                 <  1:38137/150‑1 (MQ=255)
     aGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGgaaa                                                                                                                                                  >  2:642326/1‑148 (MQ=255)
        cACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGgaaa                                                                                                                                                  >  2:1357227/1‑145 (MQ=255)
         aCTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGA‑g                                                                                                                                           >  1:88440/1‑150 (MQ=255)
         aCTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGAAAATATGA‑g                                                                                                                                           >  2:1189803/1‑150 (MQ=255)
          cTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAata                                                                                                                                                >  2:1276575/1‑145 (MQ=255)
            gTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑Gtt                                                                                                                                         <  2:1450770/149‑1 (MQ=255)
            gTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTa                                                                                                                                       <  2:164069/151‑1 (MQ=255)
              tAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAg                                                                                                                                      <  1:1742534/150‑1 (MQ=255)
                 aaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAAT‑AAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGA‑GTTTAGccccc                                                                                                                                 >  1:1511446/1‑151 (MQ=255)
                   aGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCt                                                                                                                                <  2:1734160/151‑1 (MQ=255)
                      gggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTAGCCCCCtact                                                                                                                             >  1:1386240/1‑151 (MQ=255)
                      gggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGA‑GGTTAGCCCCCtac                                                                                                                              >  2:775815/1‑150 (MQ=255)
                       ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCtactt                                                                                                                            <  2:1421844/151‑1 (MQ=255)
                             cAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGG‑GTTTAGCCCCCcactttatc                                                                                                                        >  2:1269329/1‑145 (MQ=255)
                              aGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GGTTAGCCCCCtacttact                                                                                                                         >  1:1327903/1‑147 (MQ=255)
                              aGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGA‑GTTTAGCCCCCcacttac                                                                                                                          >  2:168966/1‑146 (MQ=255)
                               gAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATg                                                                                                                    <  2:1035696/151‑1 (MQ=255)
                                aaaCGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTAGCCCCCCACTTTCTCGATg                                                                                                                    >  2:1636588/1‑150 (MQ=255)
                                  aCGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAAAGA‑GGTTAGCCCCCCACTTACTCGATg                                                                                                                    >  2:1614022/1‑148 (MQ=255)
                                   cGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCaa                                                                                                                 >  1:304259/1‑150 (MQ=255)
                                       gtgCGGATTCACGGTCAATAAAGTTTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGTCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGCATGGAAAATATAA‑GATTAGCCCCCCACTTACTCCTTGCacaac                                                                                                              >  2:383997/1‑148 (MQ=255)
                                       gtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTTGCCCCCTACTTACTCGATGCaaaa                                                                                                               >  1:831743/1‑148 (MQ=255)
                                         gCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACg                                                                                                          <  1:502661/151‑1 (MQ=255)
                                          cGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GGTTAGCCCCCCACTTACTCGATGCaaaa                                                                                                               >  2:1621658/1‑145 (MQ=255)
                                           ggATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGG‑GGTTAGCCCCCCACTTACTCGATGCAAAAGCa                                                                                                            >  2:438625/1‑147 (MQ=255)
                                               tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTTGCCCCCTACTTACTTGGTGCAAAAGCACGCCCTgg                                                                                                    >  1:1426182/1‑151 (MQ=255)
                                                cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGt                                                                                                   <  1:225312/151‑1 (MQ=255)
                                                cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATTGAAAATATGA‑GTTTTGCCCCCTACTTACTCGATGCAAAAACACGctc                                                                                                       >  1:590590/1‑147 (MQ=255)
                                                  cGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGtt                                                                                                  >  1:739514/1‑150 (MQ=255)
                                                    gTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATc                                                                                               <  2:858506/151‑1 (MQ=255)
                                                     tCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGA‑GGTTAGCCCCCTACTTTCTCGATGCAAAAGCACGCTCTGGtt                                                                                                  >  1:1337862/1‑147 (MQ=255)
                                                       aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTc                                                                                            <  1:1682166/151‑1 (MQ=255)
                                                            aGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCCCTGGTTATCGTCTTgg                                                                                        >  2:144562/1‑150 (MQ=255)
                                                             gTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgag                                                                                      <  1:604939/151‑1 (MQ=255)
                                                                   ggTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAATATGA‑GTTTAGCCCCCCCCTTACTCGATGCAAAAGCACGCCCTGGTTATCGTCTTGGAG‑ACgg                                                                                 >  2:205086/1‑149 (MQ=255)
                                                                      tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAgg                                                                            >  1:1714200/1‑151 (MQ=255)
                                                                       cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGt                                                                           <  2:1745458/151‑1 (MQ=255)
                                                                             ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTTGCCCCCCACCTACTCGCTGCAAAAGAACGCTCTGGGTATTGTCTTGGAG‑ACGGACAGGGTTAt                                                                       >  2:1295576/1‑149 (MQ=255)
                                                                              tAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTAt                                                                       <  1:1760669/148‑1 (MQ=255)
                                                                              tAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATg                                                                      <  1:851828/149‑1 (MQ=255)
                                                                              tAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATg                                                                      <  2:542803/149‑1 (MQ=255)
                                                                               aaaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGAAAATAAGA‑GTTTATCCCCCCACTTACTCGATGCGAAAACACGCGCTGGTTATCGTCTTGGAG‑ACCGACAGGGTTATGACg                                                                   >  2:284632/1‑151 (MQ=255)
                                                                               aaaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGA‑GGTTAGCCCCCCACCTACTCGATGCAAAAACACGCTCTGGGTATCGTCTTGGAG‑ACGGACCGGGTTTTGAc                                                                    >  2:320694/1‑150 (MQ=255)
                                                                               aaaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGA‑GTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCTCTGGGTATCGTCTTTGAG‑ACGGACAGGTTTATGACg                                                                   >  1:185533/1‑151 (MQ=255)
                                                                                  aGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTaa                                                                <  2:79232/151‑1 (MQ=255)
                                                                                  aGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTAGCCCCCTACTTTCTCGATGCAAAAGCACGCCCTGGGTATTGTCTTTGAG‑ACGGACAGGGTTATGACGt                                                                  >  2:190737/1‑149 (MQ=255)
                                                                                   gtgtGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGGAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGGTTATGACGTaa                                                                >  1:1447625/1‑150 (MQ=255)
                                                                                     gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATc                                                              <  2:807297/150‑1 (MQ=255)
                                                                                     gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGCGGGGGGTGGAAAATAAGACATTTCGCCCCC‑ACTTACCCGATGCAAAAAGACGCCCTGGTTATCTTCTTCGAG‑ACGCACACGGTTATTCCGTaagac                                                             >  2:1377230/1‑148 (MQ=255)
                                                                                       ggCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAGAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTg                                                           <  2:187276/151‑1 (MQ=255)
                                                                                       ggCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCt                                                            <  1:1561882/150‑1 (MQ=255)
                                                                                        gcgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTgc                                                          <  2:1091710/151‑1 (MQ=255)
                                                                                         cgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGTTGGAAAATATGT‑GTTTAGGCCCCCACTTACTCGAAGCCAAAACACGGCCCGGGTATCGTCTTGGAGAACGGA‑AGGGTTTTGACGGAAACCTgc                                                          >  2:1632931/1‑150 (MQ=255)
                                                                                          gcTTGCCGCCCGGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTgcgc                                                        <  1:1531764/151‑1 (MQ=255)
                                                                                             tGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCCTGGAG‑ACGGACAGGTTTATGACGTAATCCTGcgcg                                                       <  2:819405/149‑1 (MQ=255)
                                                                                              gccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATg                                                    <  1:234344/151‑1 (MQ=255)
                                                                                               ccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATgg                                                   <  2:841004/151‑1 (MQ=255)
                                                                                               ccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGA‑GGTTAGCCCCCCACCTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTTGGG‑ACGGGCAGGGTTATTACGGAATCCTGCGCGGTgg                                                   >  2:215092/1‑151 (MQ=255)
                                                                                                 gccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGGTTATGACGTAATCCTGCGCGATGGcc                                                 >  1:727499/1‑151 (MQ=255)
                                                                                                 gccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGA‑GGTTAGCCCCCTACTTAATCGATGCAAAAGCACGCTCTGGTTATCGGCTTGGGG‑AAGGGCAGGGTTATTACGGAATCCTGCGCGGTGGcc                                                 >  2:554403/1‑151 (MQ=255)
                                                                                                 gccCAGGACATTCAGGCAGGTCAGGCGCAGCGCATTGTGGCGGCGGGTAGCGAAAATGTGA‑ATTTAGCCCCCTAACTTCACCATTGTAAAGCACCCGCTGGGTTTCGTCCGGGAG‑AAGGACAGGATTGTGATGGAAtaca                                                            >  2:1311019/1‑137 (MQ=255)
                                                                                                   ccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAACACGCTCTGGTTATCGTCTTGGGG‑ACGGACAGGGTTATGACGTAATCCTGCGCGGTGGcc                                                 >  1:925428/1‑149 (MQ=255)
                                                                                                      ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGAtg                                            <  1:1062496/151‑1 (MQ=255)
                                                                                                      ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGAtg                                            <  1:434539/151‑1 (MQ=255)
                                                                                                         cATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTgc                                         <  2:789701/151‑1 (MQ=255)
                                                                                                         cATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATTGAAAATATGA‑GTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGGG‑AAGGACAGGGTTATGACGTAATCCTGCGCGATGGGCTGAtggg                                          >  2:825013/1‑148 (MQ=255)
                                                                                                          aTTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTgcg                                        <  1:1276661/151‑1 (MQ=255)
                                                                                                          aTTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTAGCCCCCCACTTTCTCGATGCAAAAACACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGGTTTTGACGTAATCCTGCGCGATGGCCTGATGTgcg                                        >  2:832753/1‑151 (MQ=255)
                                                                                                           ttCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGTTGGAAAATATGA‑GTTTTTTCCCCCACTTACTCGGTGGGAAAGCACGGTCCGGTTATCGTCTTTGAG‑ACGGAGAGGTTTATGATGTAATTCTGCGCGGTGGCCTGGTGTgcgc                                       >  1:405223/1‑151 (MQ=255)
                                                                                                                  aggTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCAt                                <  2:1712248/151‑1 (MQ=255)
                                                                                                                      cAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGA‑GTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCCCCCATGGtt                            >  1:209810/1‑151 (MQ=255)
                                                                                                                       aGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGA‑GGTTAGGCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGGTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGtt                            >  1:1741106/1‑150 (MQ=255)
                                                                                                                        ggCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTAt                          <  1:1707146/151‑1 (MQ=255)
                                                                                                                          cgcAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATc                         >  1:713490/1‑150 (MQ=255)
                                                                                                                          cgcAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCa                        <  1:40232/151‑1 (MQ=255)
                                                                                                                             agagCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCatat                     <  1:1675265/151‑1 (MQ=255)
                                                                                                                             agagCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCatat                     <  1:812540/151‑1 (MQ=255)
                                                                                                                               agCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATgg                   <  1:653494/151‑1 (MQ=255)
                                                                                                                                   ttGTGGCGGGGGGGATGGGAAAAATGA‑GTTTATCCCCCTACTTACTCGATGCAAAAGCACGCCCTGGTTATCGTCTTTGAG‑ACGGGCAGGGTTATGACGTAATCCTGCGCGATGGCCTGGTGTGCGCCCCCCCTGGGTTTCATATgggg                 >  2:880114/1‑149 (MQ=255)
                                                                                                                                    tgtgGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGAt               <  1:186610/150‑1 (MQ=255)
                                                                                                                                     gtgGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGAtt              <  1:511553/150‑1 (MQ=255)
                                                                                                                                      tgGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAc            >  2:281774/1‑151 (MQ=255)
                                                                                                                                        gCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAccg          <  1:1362658/151‑1 (MQ=255)
                                                                                                                                        gCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAccg          <  1:1140658/151‑1 (MQ=255)
                                                                                                                                          ggggggTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAccgcc        >  2:676147/1‑151 (MQ=255)
                                                                                                                                          ggggggTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAccgcc        >  2:1200148/1‑151 (MQ=255)
                                                                                                                                           gggggTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAccgccg       >  1:655179/1‑151 (MQ=255)
                                                                                                                                            ggggTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAccgccg       >  2:210366/1‑150 (MQ=255)
                                                                                                                                               gTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCTCCCATGGTTATCATATGGGGATTACCGCCGaaaa   <  2:1721039/151‑1 (MQ=255)
                                                                                                                                               gTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGaaa    >  2:785941/1‑150 (MQ=255)
                                                                                                                                               gTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGaaa    >  1:266701/1‑150 (MQ=255)
                                                                                                                                                tATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGAAAAc  >  1:34204/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                        
GCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG‑ACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGAAAAC  >  NC_000913/2326306‑2326600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑