Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,2770AC42.7% 216.6 / 50.6 124V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (63/8);  new base C (0/53);  total (63/61)
Fisher's exact test for biased strand distribution p-value = 7.11e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

AGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACT  >  NC_000913/3443129‑3443420
                                                                                                                                                       |                                                                                                                                               
aGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTAcc                                                                                                                                               >  2:740262/1‑151 (MQ=255)
 gACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTAccc                                                                                                                                              <  2:459924/151‑1 (MQ=255)
  aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTAcccc                                                                                                                                             >  1:843299/1‑151 (MQ=255)
   cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTAccccc                                                                                                                                            <  2:1369298/151‑1 (MQ=255)
   cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTAccccc                                                                                                                                            <  2:746748/151‑1 (MQ=255)
    gCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCg                                                                                                                                           <  1:172263/151‑1 (MQ=255)
       tAGAGTAACACAAAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTCCCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGcc                                                                                                                                         <  2:1161587/150‑1 (MQ=255)
          agTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCatat                                                                                                                                     >  2:27157/1‑151 (MQ=255)
            tAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTc                                                                                                                                   >  1:1136398/1‑151 (MQ=255)
            tAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTc                                                                                                                                   >  1:959635/1‑151 (MQ=255)
             aaCACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTc                                                                                                                                   >  2:659950/1‑150 (MQ=255)
                  aaaagggggTTGCCCGGGCTCAAAATACAGAAAATTTG‑TTGCCACCCAGTCCCAC‑CCAGTCCCCCCCCCAACCCATGACGGGATGGTCGCGGGGAAAAAAATAATACTGGAAGCGAAGATGGCCGGG‑ATTCCCCCCGCCATATTCACTTTc                                                                                                                             <  2:1519323/144‑1 (MQ=255)
                  aaaaaGCGGTTGCCCAGGCTGAAAATACAGCAAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTCCCCCCCCCGAACCATGACGCGATGGTCGCGGGAAACAAAATAATACTGGAAGCGAAGATTGCCGGG‑ATTCCCCCCGCCATATTCACTTTc                                                                                                                             <  1:1622403/149‑1 (MQ=255)
                   tAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTc                                                                                                                             >  1:146006/1‑150 (MQ=255)
                    aaagggttcgCCCGGGCTGAAAATACAGGAAATTTG‑TTGTCGCCCATTCCCAC‑CCAGTCCCCCCCCCGACCCAGGCCGCGGTGGTCGCGGGAAAAAAAAAAATACGGGAAGCGAAGATGGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAg                                                                                                                           <  2:721450/142‑1 (MQ=255)
                     aggCGGTTGCCCAGGCTAAAAATACAGCAAATTTT‑TTGTCAGCCAGTCCCAC‑CCAGTCCCCCCCCCGAACCATGACGCGATGGTCGCCGGAAACAGAAAAATACGGGAAGCGAAGTTTGCCGGG‑ATTCCCCCCGCCATATTCACTTTCAGc                                                                                                                          <  1:250848/149‑1 (MQ=255)
                     aaGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGc                                                                                                                          >  2:635053/1‑151 (MQ=255)
                       gCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCgg                                                                                                                        >  1:768255/1‑151 (MQ=255)
                         ggttcccagggCTGAAAATACAGCAAAATTT‑TTGCCACCCAGTCCCAC‑CCAGTCCCCCCCCCGACCCATGACGCGATGGTCGCCGGAAACAAAATAATACTGAAAGCGAAGTTTGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                      <  1:1069505/142‑1 (MQ=255)
                              cccGGGCTAAAAATACAGCAAAATTG‑TTGCCAGCCGTTCCCAC‑CCAGTCCCGCCCCCGACCCATGACGGGAGGGTCGCCGGAAACAAAAAAATACGGGAAGCAAAGATGGCCGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                  <  1:731581/150‑1 (MQ=255)
                               ccAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                >  2:1243347/1‑151 (MQ=255)
                               ccAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                >  2:871578/1‑151 (MQ=255)
                                cAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                               >  1:793888/1‑151 (MQ=255)
                                 aGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                                                              >  2:1363693/1‑151 (MQ=255)
                                 aGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                                                              >  1:65000/1‑151 (MQ=255)
                                  ggcggaaaaTACAGAAAATTTG‑TTGTCAGCCATTCCCAA‑CCAGTCCCGCCCCCGAACCAGGACGCGGGGGCCGCCGGAAACAAAATAATACTGGAAGCAAAGATTGCGGGG‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                <  2:1205664/142‑1 (MQ=255)
                                     gaaaaaaACAGCAAAATTG‑TTGTCGCCCAGTTCAAC‑CCAGTCCCCCCCCCAAACCATGCCGCATTGGTCGCGGGAAAAAAAATATTAGGGGAAGCAAAAATTGCGGGA‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                           <  2:1034865/147‑1 (MQ=255)
                                      gCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                         >  2:1725232/1‑151 (MQ=255)
                                        aaaaaaaagcaaaTTGTTTGCCACCCAGTCCAAC‑CCAGCCCCCCCCCCAACCAACGCCGCGATGGTCGAAGGAAAAAAAATAATTCGGGCAG‑AAAAATTGGCGGGAATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                             <  2:1779238/136‑1 (MQ=255)
                                        aaaaACAGAAAATTTG‑TTGCCA‑CCCGTTCCAACCCAGCCCCCCCCCCGACCCATGAGGGGGTGGCCGCGGGAAAAAAAAAAATCCTGGAGGCGAAAATTGCGGGG‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                         <  2:688017/149‑1 (MQ=255)
                                         aaTACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                      >  2:684660/1‑151 (MQ=255)
                                         aaTACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                      >  2:1322347/1‑151 (MQ=255)
                                             cAGCAAAATTG‑TTGTCAGCCAGTCCCAC‑CCAGTCCCCCCCCCAAACCATGACGCGATGGCCGCCGGGAACAAAATAATACTGGAAGCAAAGATTGCGGGG‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                   <  1:823856/150‑1 (MQ=255)
                                                 gAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                 >  2:323179/1‑148 (MQ=255)
                                                    aTTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                           >  1:783098/1‑151 (MQ=255)
                                                     ttg‑ttgTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                          >  2:1431255/1‑151 (MQ=255)
                                                       g‑ttgtCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                        >  1:93608/1‑151 (MQ=255)
                                                         ttgtCAGCCATTCCCAC‑CCAGTCCCCCCCCCAAACCATGCCGCGATGGTCGCGGGGAACAAAATAATACTGGAAGGGAAGTTTGCGGGA‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                        <  1:1678127/150‑1 (MQ=255)
                                                         ttgtCAGCCAGTCCCAA‑CCAGTCCCGCCCCCGAACCAGGACGGGGGGGCCGCCGGAAAAAAAATAATACTGGAAGCGAAGATGGCGGGA‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacga                                                                                         <  2:322245/149‑1 (MQ=255)
                                                         ttgtCACCCA‑TTCCAACCCAGTCCCCCCCCCGACCCATGACGCGTGGGTCGCGGGAAACAAAAAAATACTGGAAGCGAAGTTTGCCGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                        <  1:430957/150‑1 (MQ=255)
                                                          tgtCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                       >  2:874971/1‑150 (MQ=255)
                                                              aGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                  >  2:1065431/1‑151 (MQ=255)
                                                               gCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTg                                                                                 >  1:554238/1‑151 (MQ=255)
                                                                ccAGTCCCAA‑CCAGTCCCCCCCCCAACCCAGGCCGCGATGGCCGCGGGAAAAAAAAAATTACGGGAAGCGAAGTTTGCCGGA‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                  <  2:829398/149‑1 (MQ=255)
                                                                 cAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCt                                                                               >  1:404322/1‑151 (MQ=255)
                                                                 cAGTCCCAC‑CCAGCCCCCCCCCCGAACCATGACGCGAGGGTCGCGGGAAAAAAAATAATACGGAAAGCGAAGATGGCGGGG‑TTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                  <  2:1314567/148‑1 (MQ=255)
                                                                      ccAC‑CCAGTCCCCCCCCCGAACAAGACCGGGAGGGTCGCCGGAAAAAAAATAATCCTGGAAGCGAAGATTGCCGGG‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                            <  2:565767/149‑1 (MQ=255)
                                                                       cAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                         >  1:359597/1‑151 (MQ=255)
                                                                         accctgccccccccccGAACCAGGGCGCGATGGCCGCGGGAAACAAAATAATAGGGGAAGCGAAGATTGCCGGT‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                                                                          <  2:1061957/138‑1 (MQ=255)
                                                                         acccAGTCCCCCCCCCAACCCATGACGCGATGGTCCCCGGAAACAGAATAATACTGGAAGCGAAGATGGCGGGT‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                        <  1:1072904/149‑1 (MQ=255)
                                                                             aGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgcg                                                                    >  1:783968/1‑151 (MQ=255)
                                                                                 ccccccccGAACCAGGGCGCGAGGGCCGCCGGAAACAAAATAATACGGGAAGCAAAGTTGGCGGGA‑ATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTa                                                                  <  2:922641/146‑1 (MQ=255)
                                                                                    cccccGACCCAGGAGGCGAGGGTCGCCGGGAAAAAAATAATACTGGAAGCGAATATTGCGGGG‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTa                                                             <  2:235988/151‑1 (MQ=255)
                                                                                     ccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                            >  1:476158/1‑151 (MQ=255)
                                                                                           aCCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                      >  1:1362825/1‑151 (MQ=255)
                                                                                            ccATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATg                                                     >  2:378273/1‑151 (MQ=255)
                                                                                               tGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcgg                                                  >  2:528497/1‑151 (MQ=255)
                                                                                                gcggggggggTCGCGGGAAAAAAAATAATTGTGAAAGCGAAGTTTGCGGGT‑TTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg                                                   <  2:422152/141‑1 (MQ=255)
                                                                                                   gcgATGGTCGCGGGGAACAAAATAATACGGAAGGCAAAAATTGCCGGA‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg                                                   <  1:288484/146‑1 (MQ=255)
                                                                                                    cggtGGTCGCCGGGACCTGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                              >  2:1697170/4‑150 (MQ=255)
                                                                                                       tGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAcc                                          >  1:888096/1‑151 (MQ=255)
                                                                                                         gTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCAc                                        >  2:1502823/1‑151 (MQ=255)
                                                                                                          tCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACg                                       >  2:705999/1‑151 (MQ=255)
                                                                                                           cgcgggaaaaaaaataatACTGGAAGCGAAGTTTGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACg                                       <  2:514130/138‑1 (MQ=255)
                                                                                                             cgggaaaaaaaataatACTGGAAGCGAAGATTGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGc                                      <  1:473570/139‑1 (MQ=255)
                                                                                                              cGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                    >  2:760897/1‑150 (MQ=255)
                                                                                                               gggAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                   >  1:758060/1‑150 (MQ=255)
                                                                                                                ggAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                 >  2:1467788/1‑151 (MQ=255)
                                                                                                                  aaCAGAATAATACGGGAGGCGAAGATGGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                <  1:970845/150‑1 (MQ=255)
                                                                                                                     aaaataatACGGGAAGCAAAGATGGCGGGA‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                             <  1:87021/148‑1 (MQ=255)
                                                                                                                     aaaaaaataCTGGAGGGGAAGATTGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACaaa                              <  1:527304/144‑1 (MQ=255)
                                                                                                                     aGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                             >  2:970124/1‑150 (MQ=255)
                                                                                                                     aGAATAATACGGGAAGCGAAGATGGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                            <  2:1284374/151‑1 (MQ=255)
                                                                                                                        ataataCTGGAAGCGAAGATTGCGGGT‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaa                         <  1:112635/151‑1 (MQ=255)
                                                                                                                          aataCGGGAAGCAAAGATTGCCGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                       <  1:681289/151‑1 (MQ=255)
                                                                                                                           ataCTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                       >  2:1013684/1‑150 (MQ=255)
                                                                                                                           ataCGGGAAGCGAAGTTTGCGGGT‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                      <  1:188485/151‑1 (MQ=255)
                                                                                                                              cggaaaGCGAAGATTGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAg                    <  1:1404688/146‑1 (MQ=255)
                                                                                                                                ggAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                 >  1:791396/1‑151 (MQ=255)
                                                                                                                                ggAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCAATTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGAACTTGCTGACGTTTCGCGTAGATTACCACCATGCGGCGATGACCACGCTCAACAAATacaac                        >  2:793647/1‑144 (MQ=255)
                                                                                                                                ggAAGCAAAGATTGCGGGA‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                 <  1:1254307/151‑1 (MQ=255)
                                                                                                                                  aaGCGAAGATTGCCGGT‑ATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt               <  2:768220/151‑1 (MQ=255)
                                                                                                                                  aaGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt               >  1:853400/1‑151 (MQ=255)
                                                                                                                                    ggaaaGTTTGCGGTG‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGaa                  <  2:392772/143‑1 (MQ=255)
                                                                                                                                     cGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCAc            >  1:150188/1‑151 (MQ=255)
                                                                                                                                      gAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt           >  2:272288/1‑151 (MQ=255)
                                                                                                                                       aataTTGCCGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                 <  2:178545/141‑1 (MQ=255)
                                                                                                                                       aaGATTGCCGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt           <  1:593415/150‑1 (MQ=255)
                                                                                                                                       aaGATTGCCGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg          <  1:1730885/151‑1 (MQ=255)
                                                                                                                                       aaGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCa             <  1:771927/148‑1 (MQ=255)
                                                                                                                                       aaGATTGCCGGG‑ATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg          <  2:622453/151‑1 (MQ=255)
                                                                                                                                       aaGATGGCGGGT‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc              <  2:1328552/147‑1 (MQ=255)
                                                                                                                                           ttGCGGGT‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCa        <  2:783930/149‑1 (MQ=255)
                                                                                                                                           ttGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa       <  1:1358595/150‑1 (MQ=255)
                                                                                                                                           ttGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa       >  2:1020952/1‑150 (MQ=255)
                                                                                                                                           ttGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa       >  2:1176609/1‑150 (MQ=255)
                                                                                                                                               cGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact  >  1:37808/1‑151 (MQ=255)
                                                                                                                                                ggg‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCa        <  2:1551643/144‑1 (MQ=255)
                                                                                                                                                ggg‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac   <  1:659979/149‑1 (MQ=255)
                                                                                                                                                ggg‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact  >  1:1617950/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                               
AGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACT  >  NC_000913/3443129‑3443420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑