Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,473,612 A→C L157* (TTA→TGA)  rpsG ← 30S ribosomal subunit protein S7

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,473,6120AC98.2% 430.9 / ‑0.3 110L157* (TTA→TGA) rpsG30S ribosomal subunit protein S7
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (54/54);  total (55/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-02

Only 100 of 110 total aligned reads displayed.

GATACCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGA  >  NC_000913/3473470‑3473758
                                                                                                                                              |                                                                                                                                                  
gATACCGATGTTACGGTGGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCa                                                                                                                                                <  2:37596/147‑1 (MQ=255)
gATACCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAAc                                                                                                                                              <  2:842538/149‑1 (MQ=255)
 aTACCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGt                                                                                                                                           >  1:668426/1‑151 (MQ=255)
 aTACCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGt                                                                                                                                           >  2:1596172/1‑151 (MQ=255)
   aCCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTa                                                                                                                                          <  1:608365/150‑1 (MQ=255)
      gATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAgtgt                                                                                                                                      <  2:572141/151‑1 (MQ=255)
      gATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAgtgt                                                                                                                                      >  1:1659490/1‑151 (MQ=255)
            aCGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAAc                                                                                                                                >  1:367446/1‑151 (MQ=255)
             cGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAAc                                                                                                                                <  2:860955/150‑1 (MQ=255)
                   cGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgt                                                                                                                         >  1:1565642/1‑151 (MQ=255)
                    gTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgtt                                                                                                                        >  2:1314768/1‑151 (MQ=255)
                     tGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgttg                                                                                                                       >  2:1211890/1‑151 (MQ=255)
                        gATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCt                                                                                                                    <  1:999440/151‑1 (MQ=255)
                         aTGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCtt                                                                                                                   >  1:1115522/1‑151 (MQ=255)
                          tGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCtt                                                                                                                   <  1:686464/150‑1 (MQ=255)
                           gggTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTc                                                                                                                  <  1:627255/150‑1 (MQ=255)
                           gggTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCg                                                                                                                 <  2:1660528/151‑1 (MQ=255)
                            ggTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCgg                                                                                                                <  2:1011735/151‑1 (MQ=255)
                              tgttgtACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGcc                                                                                                              >  1:405098/1‑151 (MQ=255)
                               gttgtACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGcc                                                                                                              >  1:359658/1‑150 (MQ=255)
                                ttgtACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCa                                                                                                             <  2:611990/150‑1 (MQ=255)
                                ttgtACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCAt                                                                                                            >  2:1718360/1‑151 (MQ=255)
                                     cGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACgg                                                                                                        >  1:1259050/1‑150 (MQ=255)
                                      gAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTg                                                                                                      >  2:720510/1‑151 (MQ=255)
                                       aGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTg                                                                                                      >  1:839474/1‑150 (MQ=255)
                                       aGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGa                                                                                                     <  2:1368250/151‑1 (MQ=255)
                                        gCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGaa                                                                                                    <  2:65451/151‑1 (MQ=255)
                                         ccATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAAc                                                                                                   >  1:1261551/1‑151 (MQ=255)
                                          cATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACg                                                                                                  >  2:8783/1‑151 (MQ=255)
                                           aTTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGt                                                                                                 >  1:908076/1‑151 (MQ=255)
                                             ttGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCACCGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACggc                                                                                                >  1:301237/1‑148 (MQ=255)
                                             ttGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTc                                                                                                <  1:559602/150‑1 (MQ=255)
                                                 ttCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGGGCCCGCCTGGTGACTAAAACACCGAAGGGATCACCAACGGTAGTGTGCGAGCGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCa                                                                                            <  2:1086712/150‑1 (MQ=255)
                                                 ttCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCa                                                                                            <  2:1151566/150‑1 (MQ=255)
                                                 ttCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCAc                                                                                           >  2:911494/1‑151 (MQ=255)
                                                   ccTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGt                                                                                         <  2:630537/151‑1 (MQ=255)
                                                      cGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGttt                                                                                       <  1:124397/150‑1 (MQ=255)
                                                      cGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGtt                                                                                        <  2:1258964/149‑1 (MQ=255)
                                                        tttATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCtt                                                                                    >  2:620222/1‑151 (MQ=255)
                                                         ttATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCtt                                                                                    >  2:829534/1‑150 (MQ=255)
                                                           aTCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAAc                                                                                 >  1:1293692/1‑151 (MQ=255)
                                                            tCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACt                                                                                >  2:1267746/1‑151 (MQ=255)
                                                               tttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACt                                                                                <  2:118354/148‑1 (MQ=255)
                                                                ttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGc                                                                              >  1:1771057/1‑149 (MQ=255)
                                                                ttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCa                                                                             <  1:1451289/150‑1 (MQ=255)
                                                                 tAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGt                                                                           >  1:1323621/1‑151 (MQ=255)
                                                                   ggCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTAc                                                                         >  1:1623753/1‑151 (MQ=255)
                                                                    gCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTAcc                                                                        <  2:138224/151‑1 (MQ=255)
                                                                     cGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCt                                                                       >  1:1475136/1‑151 (MQ=255)
                                                                      gTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCtt                                                                      >  2:840684/1‑151 (MQ=255)
                                                                       ttCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCttt                                                                     >  2:436991/1‑151 (MQ=255)
                                                                        tCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCttt                                                                     <  2:1642797/150‑1 (MQ=255)
                                                                         cAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGt                                                                   >  1:596862/1‑151 (MQ=255)
                                                                         cAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGt                                                                   >  1:83724/1‑151 (MQ=255)
                                                                           aTTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGttt                                                                 <  2:1055560/151‑1 (MQ=255)
                                                                           aTTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGttt                                                                 <  1:1762837/151‑1 (MQ=255)
                                                                              tAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTc                                                               <  2:67259/150‑1 (MQ=255)
                                                                               aaGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTg                                                             <  2:1612470/151‑1 (MQ=255)
                                                                               aaGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTg                                                             >  1:1117609/1‑151 (MQ=255)
                                                                                aGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgc                                                            <  1:331497/151‑1 (MQ=255)
                                                                                   aGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgcag                                                          <  2:500067/150‑1 (MQ=255)
                                                                                    gCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgcagca                                                        <  2:164325/151‑1 (MQ=255)
                                                                                     cccAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATGCGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCAt                                                       <  2:21503/151‑1 (MQ=255)
                                                                                      ccAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATc                                                      <  1:1615596/151‑1 (MQ=255)
                                                                                       cAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATc                                                      >  2:1281343/1‑150 (MQ=255)
                                                                                         aaGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATc                                                      >  1:1165545/1‑148 (MQ=255)
                                                                                           gCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGaaa                                                 <  2:393675/151‑1 (MQ=255)
                                                                                            cGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGaa                                                  >  2:1718514/1‑149 (MQ=255)
                                                                                             gggCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgt                                               >  2:679182/1‑151 (MQ=255)
                                                                                               gctgctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgttc                                             <  2:97800/151‑1 (MQ=255)
                                                                                                  gctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgttcgtt                                          <  2:1027996/151‑1 (MQ=255)
                                                                                                  gctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgttcgtt                                          <  2:821100/151‑1 (MQ=255)
                                                                                                  gctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgttcgt                                           <  1:1594167/150‑1 (MQ=255)
                                                                                                  gctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgttcg                                            <  1:710294/149‑1 (MQ=255)
                                                                                                    ttACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGttcgttcg                                        >  2:1727996/1‑151 (MQ=255)
                                                                                                     tACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGttcgttcg                                        <  2:1441347/150‑1 (MQ=255)
                                                                                                     tACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGc                                       >  1:1645293/1‑151 (MQ=255)
                                                                                                      aCTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGcc                                      <  2:948181/151‑1 (MQ=255)
                                                                                                      aCTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGcc                                      >  2:750701/1‑151 (MQ=255)
                                                                                                            aGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGgcgc                                <  1:1034522/151‑1 (MQ=255)
                                                                                                                cccgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCag                              >  2:120539/1‑149 (MQ=255)
                                                                                                                 ccgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGc                           >  1:1058674/1‑151 (MQ=255)
                                                                                                                 ccgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGc                           <  1:1527951/151‑1 (MQ=255)
                                                                                                                  cgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGcc                          >  2:346406/1‑151 (MQ=255)
                                                                                                                   gccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGcc                          >  1:642238/1‑150 (MQ=255)
                                                                                                                   gccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGcc                          >  1:157028/1‑150 (MQ=255)
                                                                                                                   gccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCa                         <  2:1565507/151‑1 (MQ=255)
                                                                                                                    ccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCAt                        >  1:742984/1‑151 (MQ=255)
                                                                                                                    ccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCAt                        >  2:867031/1‑151 (MQ=255)
                                                                                                                      tGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATgg                      >  2:264418/1‑151 (MQ=255)
                                                                                                                         tGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGAtt                   >  1:431189/1‑151 (MQ=255)
                                                                                                                         tGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGAtt                   >  2:778825/1‑151 (MQ=255)
                                                                                                                          gACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGAttt                  <  2:474590/151‑1 (MQ=255)
                                                                                                                           aCTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGAttt                  >  1:905391/1‑150 (MQ=255)
                                                                                                                                aaCTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCAcc            <  1:441642/151‑1 (MQ=255)
                                                                                                                                  cTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACcgc          >  2:650585/1‑151 (MQ=255)
                                                                                                                                       aaGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTa     <  2:1441974/151‑1 (MQ=255)
                                                                                                                                          ggATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGa  <  1:194346/151‑1 (MQ=255)
                                                                                                                                          ggATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGa  <  2:1293602/151‑1 (MQ=255)
                                                                                                                                           gATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACg   >  1:740233/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
GATACCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGA  >  NC_000913/3473470‑3473758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: