Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,025,435 T→A intergenic (+222/‑76) yodD → / → yedP uncharacterized protein/putative mannosyl‑3‑phosphoglycerate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,025,4350TA99.1% 479.5 / ‑3.8 116intergenic (+222/‑76)yodD/yedPuncharacterized protein/putative mannosyl‑3‑phosphoglycerate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (57/58);  total (58/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 116 total aligned reads displayed.

CGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTT  >  NC_000913/2025288‑2025580
                                                                                                                                                   |                                                                                                                                                 
  ttACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTc                                                                                                                                              >  2:1625000/1‑151 (MQ=255)
   tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTc                                                                                                                                              <  2:958090/150‑1 (MQ=255)
    acacCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTccc                                                                                                                                            >  2:530535/1‑151 (MQ=255)
      acCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCg                                                                                                                                           >  1:441837/1‑150 (MQ=255)
        cAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGaaa                                                                                                                                        <  2:440377/151‑1 (MQ=255)
         aGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGaaaa                                                                                                                                       <  1:836751/151‑1 (MQ=255)
         aGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGaaaa                                                                                                                                       <  2:380846/151‑1 (MQ=255)
         aGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGaaa                                                                                                                                        >  2:1572048/1‑150 (MQ=255)
          ggAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCCCGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGaaaaa                                                                                                                                      <  2:1220423/151‑1 (MQ=255)
            aaaTCTGATGTGTTCATCACCTTTTCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATTTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAat                                                                                                                                    <  2:1538684/151‑1 (MQ=255)
            aaaTCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAat                                                                                                                                    <  2:667618/151‑1 (MQ=255)
               tCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAatcat                                                                                                                                 >  1:551675/1‑151 (MQ=255)
                 tGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCt                                                                                                                               >  1:1399277/1‑151 (MQ=255)
                 tGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCt                                                                                                                               >  2:1439031/1‑151 (MQ=255)
                  gATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAatcatc                                                                                                                                <  1:5325/149‑1 (MQ=255)
                  gATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAatca                                                                                                                                  <  2:1050136/147‑1 (MQ=255)
                  gATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCtt                                                                                                                              >  2:728750/1‑151 (MQ=255)
                       gtTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATa                                                                                                                         <  1:889368/151‑1 (MQ=255)
                       gtTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATa                                                                                                                         <  2:1382964/151‑1 (MQ=255)
                         tcatcaCCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAGTGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATaa                                                                                                                        <  1:734600/150‑1 (MQ=255)
                             caCCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTtgt                                                                                                                   >  2:1255762/1‑151 (MQ=255)
                             caCCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTtgt                                                                                                                   >  2:1697762/1‑151 (MQ=255)
                              aCCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTtgtg                                                                                                                  >  1:1078532/1‑151 (MQ=255)
                               ccTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTtgtg                                                                                                                  <  2:616983/150‑1 (MQ=255)
                                  tATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCg                                                                                                                <  1:547674/149‑1 (MQ=255)
                                   aTCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGtt                                                                                                             <  1:399605/151‑1 (MQ=255)
                                   aTCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGt                                                                                                              <  1:1189776/150‑1 (MQ=255)
                                   aTCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCAACCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGt                                                                                                              <  2:540644/150‑1 (MQ=255)
                                    tCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGttt                                                                                                            >  2:810812/1‑151 (MQ=255)
                                     ccGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGttt                                                                                                            <  2:586231/150‑1 (MQ=255)
                                            ttttttCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAat                                                                                                     <  1:1025551/150‑1 (MQ=255)
                                             tttttCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAatc                                                                                                    >  2:1712598/1‑150 (MQ=255)
                                                ttCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATcc                                                                                                >  1:370573/1‑151 (MQ=255)
                                                  cGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCt                                                                                               <  1:1634109/150‑1 (MQ=255)
                                                      gACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTaa                                                                                          <  1:1278240/151‑1 (MQ=255)
                                                      gACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTaa                                                                                          <  1:230325/151‑1 (MQ=255)
                                                      gACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTa                                                                                           >  1:994870/1‑150 (MQ=255)
                                                        cAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTa                                                                                           >  1:1724131/1‑148 (MQ=255)
                                                         aaGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAag                                                                                         >  1:735268/1‑149 (MQ=255)
                                                             aaaTATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGt                                                                                   <  1:356776/151‑1 (MQ=255)
                                                             aaaTATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGt                                                                                   <  1:1684194/151‑1 (MQ=255)
                                                              aaTATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGt                                                                                   >  1:1326018/1‑150 (MQ=255)
                                                                  ttCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTgc                                                                              >  1:408617/1‑151 (MQ=255)
                                                                     gCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGAGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTCTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGcgcg                                                                           >  2:1162415/1‑151 (MQ=255)
                                                                       cATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGAc                                                                         >  1:388225/1‑151 (MQ=255)
                                                                        atatGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACt                                                                        <  1:93531/151‑1 (MQ=255)
                                                                          atgaatgaTTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTAt                                                                      >  1:65521/1‑151 (MQ=255)
                                                                           tgaatgaTTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGGGTACTGCGCGACTATg                                                                     >  2:1580576/1‑151 (MQ=255)
                                                                           tgaatgaTTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATg                                                                     >  2:144585/1‑151 (MQ=255)
                                                                            gaatgaTTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATg                                                                     <  2:726617/150‑1 (MQ=255)
                                                                             aatgaTTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGtt                                                                   >  1:49758/1‑151 (MQ=255)
                                                                              atgaTTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGttt                                                                  >  2:1649382/1‑151 (MQ=255)
                                                                              atgaTTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGttt                                                                  >  1:124465/1‑151 (MQ=255)
                                                                              atgaTTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGttt                                                                  >  1:1307668/1‑151 (MQ=255)
                                                                               tgaTTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGtttt                                                                 >  1:63554/1‑151 (MQ=255)
                                                                               tgaTTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGtttt                                                                 >  1:1042333/1‑151 (MQ=255)
                                                                                gaTTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTttc                                                                >  1:33350/1‑151 (MQ=255)
                                                                                  ttAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTttca                                                               >  1:719315/1‑150 (MQ=255)
                                                                                    aaCGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTttcaatt                                                            >  1:599544/1‑151 (MQ=255)
                                                                                    aaCGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTttcaatt                                                            <  2:580055/151‑1 (MQ=255)
                                                                                       gCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTttcaattca                                                          >  1:61223/1‑150 (MQ=255)
                                                                                          aCTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTcaaca                                                       <  1:1031917/150‑1 (MQ=255)
                                                                                            ttATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAAcc                                                    >  2:1682997/1‑151 (MQ=255)
                                                                                             tATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAAcc                                                    >  2:1563174/1‑150 (MQ=255)
                                                                                              aTGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCac                                                  <  2:1354208/151‑1 (MQ=255)
                                                                                                gAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCact                                                 >  1:206315/1‑150 (MQ=255)
                                                                                                gAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCaat                                                 <  1:1768694/150‑3 (MQ=255)
                                                                                                  aTAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCactact                                              >  2:408455/1‑151 (MQ=255)
                                                                                                   tAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTATTGCGCGACTATGTTTTCAATTCAACAACCACTACTg                                             >  1:1481341/1‑151 (MQ=255)
                                                                                                    aGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTgg                                            >  1:1103622/1‑151 (MQ=255)
                                                                                                        aTGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGtttt                                        >  2:1318559/1‑151 (MQ=255)
                                                                                                        aTGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGtttt                                        <  1:1035916/151‑1 (MQ=255)
                                                                                                          gtgACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGttttt                                       <  2:437457/150‑1 (MQ=255)
                                                                                                               gCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGAt                                 <  1:1384559/151‑1 (MQ=255)
                                                                                                                cACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATc                                <  1:242476/151‑1 (MQ=255)
                                                                                                                 aCGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCt                               <  2:838707/151‑1 (MQ=255)
                                                                                                                  cGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCtt                              <  1:771493/151‑1 (MQ=255)
                                                                                                                   gTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTg                             <  2:1527804/151‑1 (MQ=255)
                                                                                                                     cACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGAt                           <  1:1769786/151‑1 (MQ=255)
                                                                                                                     cACCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGAt                           >  2:392790/1‑151 (MQ=255)
                                                                                                                      aCCCCTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATg                          >  2:1310244/1‑151 (MQ=255)
                                                                                                                       ccccTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATgg                         >  1:769108/1‑151 (MQ=255)
                                                                                                                        cccTTGGTGGCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGc                        >  2:1570186/1‑151 (MQ=255)
                                                                                                                            tggtggCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCAccc                    >  1:853356/1‑151 (MQ=255)
                                                                                                                            tggtggCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCAccc                    >  2:1192830/1‑151 (MQ=255)
                                                                                                                             ggtggCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCct                   <  1:472528/151‑1 (MQ=255)
                                                                                                                              gtggCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCctg                  <  2:1050257/151‑1 (MQ=255)
                                                                                                                                ggCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCctgct                <  2:1032792/151‑1 (MQ=255)
                                                                                                                                 gCTCTGGCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCctgctg               <  1:622715/151‑1 (MQ=255)
                                                                                                                                      ggCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGAc            >  2:667780/1‑149 (MQ=255)
                                                                                                                                      ggCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACa           <  1:1103486/150‑1 (MQ=255)
                                                                                                                                      ggCGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACa           <  1:780152/150‑1 (MQ=255)
                                                                                                                                        cGTCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTc        >  1:1777363/1‑151 (MQ=255)
                                                                                                                                          tCAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCAt      >  1:861285/1‑151 (MQ=255)
                                                                                                                                           cAGCGGGATCGCTCCCGAAAAAATCATCTTTGATAATCTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCAt      >  1:1449765/1‑150 (MQ=255)
                                                                                                                                           cAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCAt      <  1:656628/150‑1 (MQ=255)
                                                                                                                                           cAGCGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATa     <  2:8379/151‑1 (MQ=255)
                                                                                                                                              cGGGAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGtt  >  1:497046/1‑151 (MQ=255)
                                                                                                                                               gggAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGtt  >  2:1774372/1‑150 (MQ=255)
                                                                                                                                               gggAACGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGtt  >  2:1714104/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTT  >  NC_000913/2025288‑2025580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: