Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,807,248	+T	coding (117/993 nt)	proX →	glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,807,247	1	.	T	100.0%	362.5 / NA	93	T39I (ACT→ATT)	proX	glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (54/39);  total (54/39)

CGGGCATTATCACCCTGCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCAC‑TGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGAC  >  NC_000913/2807097‑2807391                                                                                                                                                         |                                                                                                                                                 cGGGCATTATCACCCTGCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCAc                                                                                                                                                   >  2:860207/1‑151 (MQ=255) cGGGCATTATCACCCTGCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCAc                                                                                                                                                   <  1:550870/151‑1 (MQ=255)  gggCATTATCACCCTGCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCAC‑t                                                                                                                                                 >  2:732036/1‑151 (MQ=255)    gCATTATCACCCTGCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCAC‑ttg                                                                                                                                               >  2:93325/1‑149 (MQ=255)     cATTATCACCCTGCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCAC‑ttg                                                                                                                                               >  2:631196/1‑148 (MQ=255)      aTTATCACCCTGCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCAC‑ttgaa                                                                                                                                             <  2:778933/151‑5 (MQ=255)      aTTATCACCCTGCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCAC‑ttga                                                                                                                                              >  2:442077/1‑147 (MQ=255)         aTCACCCTGCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTgaaga                                                                                                                                            >  1:188661/1‑150 (MQ=255)          tCACCCTGCCAAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTgaaga                                                                                                                                            >  1:520558/1‑150 (MQ=255)           cACCCTGCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAAc                                                                                                                                         >  2:604204/1‑151 (MQ=255)              ccTGCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCtt                                                                                                                                      >  2:844655/1‑151 (MQ=255)                 gCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCa                                                                                                                                   <  1:616865/151‑1 (MQ=255)                      aaaaaaGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACg                                                                                                                               <  2:210264/151‑1 (MQ=255)                       aaaaaGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACgc                                                                                                                              >  2:160995/1‑151 (MQ=255)                          aaGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACgctgc                                                                                                                           >  1:439494/1‑151 (MQ=255)                           aGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGCGCACCATCACTTGAAGAAACCTTCCAGACgctgct                                                                                                                          >  2:1342506/1‑151 (MQ=255)                           aGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACgctgct                                                                                                                          >  1:279648/1‑151 (MQ=255)                               aTAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTc                                                                                                                      >  1:18092/1‑151 (MQ=255)                                 aaCAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTc                                                                                                                      >  1:218366/1‑149 (MQ=255)                                  aCAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGt                                                                                                                   >  1:506014/1‑151 (MQ=255)                                   cAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTc                                                                                                                  <  2:306546/151‑1 (MQ=255)                                   cAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTc                                                                                                                  <  2:1093743/151‑1 (MQ=255)                                      tGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGAACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTg                                                                                                               >  2:890875/1‑151 (MQ=255)                                      agCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTg                                                                                                               >  1:117010/2‑151 (MQ=255)                                       gCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTgc                                                                                                              >  1:1148503/1‑151 (MQ=255)                                       gCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTgc                                                                                                              <  1:406780/151‑1 (MQ=255)                                       gCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTg                                                                                                               <  1:12697/150‑1 (MQ=255)                                        cGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTgcg                                                                                                             >  1:210817/1‑151 (MQ=255)                                         gACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTgcgc                                                                                                            >  1:1437822/1‑151 (MQ=255)                                            aTAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTgg                                                                                                         >  2:976283/1‑151 (MQ=255)                                             tAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCt                                                                                                           >  2:1289603/1‑148 (MQ=255)                                             tAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTgg                                                                                                         >  2:932007/1‑150 (MQ=255)                                              aGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGga                                                                                                        <  2:986352/150‑1 (MQ=255)                                              aGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGga                                                                                                        <  1:667749/150‑1 (MQ=255)                                                cGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGaa                                                                                                     <  2:277604/151‑1 (MQ=255)                                                cGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGaa                                                                                                     <  1:1337896/151‑1 (MQ=255)                                                  tACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAAt                                                                                                   >  1:387675/1‑151 (MQ=255)                                                  tACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAAt                                                                                                   >  2:1302032/1‑151 (MQ=255)                                                  tACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAAt                                                                                                   >  1:122526/1‑151 (MQ=255)                                                      ttttGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTa                                                                                                 <  1:135490/149‑1 (MQ=255)                                                         tGCGACAGCGTTTGCCACGCTTATCTCTACACAATCTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCAGCTCTTGAAGAAGCCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGt                                                                                              <  2:652936/149‑1 (MQ=255)                                                         tGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGtt                                                                                             >  1:960489/1‑150 (MQ=255)                                                         tGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGCGCGCTGGAGAAATTAgg                                                                                               >  1:140050/1‑148 (MQ=255)                                                             aCAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATAcc                                                                                        <  1:768916/151‑1 (MQ=255)                                                              cAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCg                                                                                       <  2:326036/151‑1 (MQ=255)                                                               aGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGt                                                                                      >  2:72612/1‑151 (MQ=255)                                                                 cGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTca                                                                                    <  1:436690/151‑1 (MQ=255)                                                                    ttGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTcaac                                                                                  <  1:1230796/150‑1 (MQ=255)                                                                    ttGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTcaac                                                                                  <  1:15802/150‑1 (MQ=255)                                                                        cACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAAcc                                                                             <  2:70767/151‑1 (MQ=255)                                                                        cACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAAcc                                                                             <  2:744305/151‑1 (MQ=255)                                                                          cGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCa                                                                           <  2:590329/151‑1 (MQ=255)                                                                              tATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGc                                                                         <  2:1395238/149‑1 (MQ=255)                                                                               aTCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGa                                                                       >  2:1417520/1‑150 (MQ=255)                                                                                 ctctACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGt                                                                    >  1:1408450/1‑151 (MQ=255)                                                                                  tctACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGt                                                                    >  2:679984/1‑150 (MQ=255)                                                                                    tACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGt                                                                    >  1:1277913/1‑148 (MQ=255)                                                                                      cacaAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGAtt                                                               >  2:1171613/1‑151 (MQ=255)                                                                                        caAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTAc                                                             >  1:29087/1‑151 (MQ=255)                                                                                         aaaCTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTAc                                                             >  2:1026222/1‑150 (MQ=255)                                                                                         aaaCTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTAc                                                             <  1:1351466/150‑1 (MQ=255)                                                                                          aaCTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACaa                                                           <  1:464105/151‑1 (MQ=255)                                                                                              tttGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGt                                                        <  2:405145/150‑1 (MQ=255)                                                                                                tgctgcCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTg                                                      <  1:412304/150‑1 (MQ=255)                                                                                                 gctgcCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTgg                                                     <  2:339310/150‑1 (MQ=255)                                                                                                   tgcCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCt                                                   >  1:1355549/1‑150 (MQ=255)                                                                                                    gcCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTac                                                 <  2:322640/151‑1 (MQ=255)                                                                                                    gcCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTa                                                  <  2:841398/150‑1 (MQ=255)                                                                                                       gATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACAcc                                              >  1:161038/1‑151 (MQ=255)                                                                                                       gATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACAcc                                              >  2:186128/1‑151 (MQ=255)                                                                                                            gCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACAcc                                              <  2:1066383/146‑1 (MQ=255)                                                                                                            gCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACAcc                                              <  1:88916/146‑1 (MQ=255)                                                                                                               gggCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCgcttgc                                      >  1:548054/1‑151 (MQ=255)                                                                                                                ggCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCgcttgct                                     <  1:253695/151‑1 (MQ=255)                                                                                                                 gCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCgcttgctt                                    >  2:1037569/1‑151 (MQ=255)                                                                                                                  cAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTc                                   >  2:127972/1‑151 (MQ=255)                                                                                                                  cAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTc                                   <  1:789652/151‑1 (MQ=255)                                                                                                                   aaaGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTcc                                  >  2:1200648/1‑151 (MQ=255)                                                                                                                   aaaGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTcc                                  >  1:105938/1‑151 (MQ=255)                                                                                                                    aaGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCgcttgctt                                    >  1:178078/1‑148 (MQ=255)                                                                                                                     aGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCgg                                >  1:1101201/1‑151 (MQ=255)                                                                                                                      ggCATTACTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGc                               <  2:869259/151‑1 (MQ=255)                                                                                                                            aCTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGc                          >  2:901011/1‑150 (MQ=255)                                                                                                                            aCTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGc                          >  2:363254/1‑150 (MQ=255)                                                                                                                             cTGTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCa                         >  2:887588/1‑150 (MQ=255)                                                                                                                               gTTAATCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCaa                        <  2:1394990/149‑1 (MQ=255)                                                                                                                                  aaTCCAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCtt                    <  1:735192/150‑1 (MQ=255)                                                                                                                                     ccAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCAc                 <  2:554549/150‑1 (MQ=255)                                                                                                                                     ccAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCAc                 <  1:840303/150‑1 (MQ=255)                                                                                                                                      cAGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCAccg               >  1:886454/1‑151 (MQ=255)                                                                                                                                       aGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCAccgc              >  1:986092/1‑151 (MQ=255)                                                                                                                                       aGTTCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCAccgc              >  1:1117170/1‑151 (MQ=255)                                                                                                                                         ttCAGAGCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCAccgcc             <  1:765569/150‑1 (MQ=255)                                                                                                                                            agagCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTg          >  2:737441/1‑150 (MQ=255)                                                                                                                                              agCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAAc       >  2:387777/1‑151 (MQ=255)                                                                                                                                              agCACCATCACTTAAAGAACCCTCCCAGACGCTGCTGGTCAGTCGTGCGCTGGAAAAATTAGTTTATCCCTTCAACAACCCCAGCGAATTAAATTACACCGTGGGCTACACCTCGCTTGCTCCCGGCGATGCACCCTTCACCGCCGTGaa        <  1:160989/150‑1 (MQ=255)                                                                                                                                               gCACCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACt      >  1:86808/1‑151 (MQ=255)                                                                                                                                                 aCCATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTgg    >  2:65598/1‑151 (MQ=255)                                                                                                                                                   cATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGAc  >  1:747532/1‑151 (MQ=255)                                                                                                                                                   cATCACTTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGAc  >  1:1286986/1‑151 (MQ=255)                                                                                                                                                         |                                                                                                                                                 CGGGCATTATCACCCTGCC‑AAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCAC‑TGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGATTACAACGTTGGCTACACCTCGCTTGCTTCCGGCGATGCAACCTTCACCGCCGTGAACTGGAC  >  NC_000913/2807097‑2807391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑