Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913826,9420AC42.4% 85.9 / 13.6 59V104G (GTG→GGG) ybhSputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (24/10);  new base C (0/25);  total (24/35)
Fisher's exact test for biased strand distribution p-value = 9.62e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGCATTTGCCAGATCTGCCAGATCCCTTCGACATACCCCTGTACAAAGTTAGCGGTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCGCTACGCTGTTCCAGTAAAATCCCGACCC  >  NC_000913/826796‑827089
                                                                                                                                                   |                                                                                                                                                     
gctGCATTTGCCAGATCTGCCAGATCCCTTCGACATACCCCTGTACAAAGTTAGCGGTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAaccacc                                                                                                                                                     >  1:163531/1‑149 (MQ=255)
         gccagatctgccagatcCCTTCGACATACCCCTGTACAAAGTTAGCGGTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAAt                                                                                                                                         <  2:406665/151‑1 (MQ=255)
            agatctgccagatcCCTTCGACATACCCCTGTACAAAGTTAGCGGTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAAtttt                                                                                                                                      >  1:1233350/1‑151 (MQ=255)
                  gccagatcCCTTCGACATACCCCTGTACAAAGTTAGCGGTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCg                                                                                                                                <  1:180448/150‑1 (MQ=255)
                  gccagatcCCTTCGACATACCCCTGTACAAAGTTAGCGGTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAACAACCACCAGACCGCGAATTTTCCCCg                                                                                                                                <  2:863057/150‑1 (MQ=255)
                         cccTTCGACATACCCCTGTACAAAGTTAGCGGTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCAtt                                                                                                                        >  1:379963/1‑151 (MQ=255)
                          ccTTCGACATACCCCTTTACAAAGTTAGGGGTATTCGGCCCACTGCCGTCGGTAATCCCCTGAATCGGTGCGGTGGCGTTGGCGCCCCCCATCTGTTCCGCAAAACCCACCGAAAAACCCCCCAGCCCGCAATTTTTCCCCGCCTGCAttt                                                                                                                       <  1:201428/151‑1 (MQ=255)
                           cTTCGACATACCCCTGTACAAAGTTAGCGGGATTCGGCTAACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCAtttt                                                                                                                      >  2:1396736/1‑151 (MQ=255)
                                 cccaCCCCGGAACAAGTTTCGCGGTTTTCGTCTCACTCCCGTGGGTGATCACCTAAACCGTTGCGGGGGCGTTGCCGCGCTTCTTCTCTCCCCAAAACTCACCCGAAATAGCCACCAGCCCTAGAATTTTCCCCGCCTGCATTTTGGCGAt                                                                                                                <  1:380090/148‑1 (MQ=255)
                                    cccccTGTACAAAGTTAGCGGTATTCGGCTCACGGCCGTCGGTGATCACCTGAATCGGTGCGGGGGCGTTGGCGCGCTCCATCTGTTCCGCAAAACCCCCCGGAATACCCCCCAGCCCGCGAATTTCCCCCGCCTGCATTTTGGCGATc                                                                                                               <  2:543679/148‑1 (MQ=255)
                                     ccccTTTACAAAGTTAGCGGTATTCGGCTCCCTGCCGTCGGTAATCACCTGAATCGGGGGGGTGGCGTGGGCGCGCTCCATCTGTCCCAAAAAACCCCCGGGAATAACCACCAGACCGCAATTTTTCCCCGCCTGCATTTTGGCGATCAGt                                                                                                            <  2:959947/151‑1 (MQ=255)
                                     ccccTGAACAAAGTTGGGGTTATTCGGCTCACGGCCGTGGGTGACCCCCTGAATCGGGGGGGGGGCTTGGGCGCCCTCCATCTGTCCCGAAAAACCCCCGGAAATACCCCCCAGACCGCGATTTTTCCCCGCCTGCATTTTGGCGATCa                                                                                                              <  1:1418694/149‑1 (MQ=255)
                                      cccTGTACAAAGTTAGCGGTATTCGGCCCCCGGCCGCCGTTGATCCCCTGAAGCGGTGCGGTGGCGTTGGCGCGCCCCATCTGTCCCGCAAAATCCACGGAAAAACCCCCAAGCCCGCGATTTTTCCCCGCCTGCATTTTGGCGATCAGtt                                                                                                           <  2:243694/151‑1 (MQ=255)
                                          gTACAAAGTTAGCGGTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTg                                                                                                       >  2:624339/1‑151 (MQ=255)
                                            aCAAATTTAGCGTTATGCGGCCCCCTGCCGTCGGTGATCCCCTGAACCGGTGCGGGGGCTTGGGCGCGCTCCATCTTTCCCACAAAACCCCCCGAAATACCCCCCAGCCCGCGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGAc                                                                                                     <  1:1241821/151‑1 (MQ=255)
                                            aCAAAGTTGGCGGTATTCGGCTCCCTGCCGTCGTTGATCACCTGAATGGGTGGGGTGGCGTTGGCGCGCTCCATCTGTCCCGAAAAACCCACCGAAATAACCCCCAGACCGCGATTTTTCCCCGCCTGCATTTTGGCGATCAGTTcc                                                                                                         <  2:759177/147‑1 (MQ=255)
                                             cAAAGTTAGCGGCATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACg                                                                                                    >  2:885479/1‑151 (MQ=255)
                                              aaaGTTGGGGGTATCCGCCTCCCTCCCGTCGGTAATCACCTAAATCGGTGCGGGGGCGTTGGCGCCCTCCATCTGTCCCAAAAAATCCACCGGAATACCCCCCAGCCCGCGAATTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGa                                                                                                      <  1:919774/148‑1 (MQ=255)
                                              aaaGTTGGCGGTATTCGCCTCACGGCCTTCGGTGACCACCTGGTTCGGGGGGGTGGCGTGGGCCCCCTCCATCTGTCCCGAAAAACCCACCGAAATAACCACCAGCCCGCGATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACg                                                                                                    <  1:1349032/150‑1 (MQ=255)
                                              aaaGTTAGCGTTTTTCGGCTCACTGCGGTCGGTGATCCCCTGAAGCGGGGCGGGGGGGTGGGCGCGCTCAATCTGTCCCGAAAAACCCCCCGGAATACCCACCAGCCCGCGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGa                                                                                                      <  2:1334022/148‑1 (MQ=255)
                                              aaaGTTAGCGGTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACgg                                                                                                   >  1:1452717/1‑151 (MQ=255)
                                              aaaGTTAGCGGTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACgg                                                                                                   >  1:475587/1‑151 (MQ=255)
                                              aaaGTTAGCGGGATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGAGATCAGTTCCTGACgg                                                                                                   >  2:408909/1‑151 (MQ=255)
                                                  ttAGCGTTATTCGGCTCCCTGCCGTCGGTGATCCCCTGAATCGGGGCGGTGGCGTTGGCGCGCTCCATCTGTCCCGCAAAACCCCCCGAAATACCCCCCAGACCGCAATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTa                                                                                                <  2:569565/150‑1 (MQ=255)
                                                  ttAGCGGTTTTCGGCTCCCTGCCGTGGGTGATCCCCGGAATCGGTGGGGGGGGGTTGGCGCGCCCCATCTGTCCCGAAAAACCCCCCGAAATACCCCCCAGCCCGCGAATTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGtt                                                                                                 <  2:583115/149‑1 (MQ=255)
                                                    aGCGGTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCACGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCg                                                                                             >  1:927723/1‑151 (MQ=255)
                                                       gtttttCGGCTCCCTGCCGTCGGTGACCACCGAAATCGGTGCGGGGGGGTTGGCGCGCTCCATCTGTCCCGAAAAACCCCCCGAAATACCCCCCAGCCCGCGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTAt                                                                                               <  2:1162462/142‑1 (MQ=255)
                                                       ggTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTg                                                                                          >  2:822358/1‑151 (MQ=255)
                                                         tATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGAt                                                                                        >  2:1312208/1‑151 (MQ=255)
                                                              gcctcccggCCGTGGGTAATCACCTGATTGGGGGCGGTGGCGTGGGCGCGCCCCATCTGTCCCGAAAAACCCCCCGAAAAACCCACCAGCCCGCGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGAtggtgg                                                                                   <  2:1233385/143‑1 (MQ=255)
                                                               gCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGc                                                                                  >  1:1400934/1‑151 (MQ=255)
                                                                ctcccggCCGTGGGTGACCCCCTGATGGGGGGGGGTGGCGTGGGCGCCCCCCATCTTTCCCGAAAAACCCCCGGAAAAACCCCCCAGCCCGCAATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGAtg                                                                                       <  2:766285/139‑1 (MQ=255)
                                                                ctcccgcccGTCGGTAACCCCCTGAATCGGTGGGGGGGCGTGGCCGCCCTCCTTTTGTCCCGAAAAATCCCCGGAAATACCCCCCAGCCCGCAATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGAtggtgg                                                                                   <  1:725146/142‑1 (MQ=255)
                                                                ctccccgCCGTGGGTGACCCCCTAAACGGGGGCGGTGGCGTTGGCGCGCTCCATCTTTCCCAAAAAACCCACCGAAATACCCCCCAGCCCGCGATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGAtggtg                                                                                    <  1:499980/142‑1 (MQ=255)
                                                                 tCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGt                                                                                >  2:670327/1‑151 (MQ=255)
                                                                  cACTGCCGTCGGTGATCCCCTGAATCGGTGCGGTGGCGTTGGCGCCCTCCTTCTGTCCCGCAAACCCCCCGGAAATAACCCCCAGCCCGCAATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTc                                                                               <  1:145197/151‑1 (MQ=255)
                                                                   aCTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCg                                                                              >  1:307350/1‑151 (MQ=255)
                                                                        cGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGt                                                                          >  1:442996/1‑150 (MQ=255)
                                                                            gttGATCCCCGGAACCGGTGCGGTGGCGTTGGCCCGCTCCATTTGTCCCCAAAAACCCACGGGAATACCCCCCAGCCCGCAATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAg                                                                        <  1:1040549/146‑1 (MQ=255)
                                                                            ggTGATCCCCTGAATCGGTGCGGTGGCGTGGGCGCGCCCCATCTTTCCCGAAAAACCCCCCGAAATAACCCCCAGCCCGCAATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGc                                                                     <  1:1437437/151‑1 (MQ=255)
                                                                            ggTGATCCCCTGAACCGGGGGGGGGCCGTGGGCGCGCCCCTTCTTTCCCGAAAAACCCCCCGAAATACCCCCCAGCCCGCGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTa                                                                         <  1:768764/147‑1 (MQ=255)
                                                                                  ccccTGAATCGGTGCGGTGGCGGTGGCGCGCTCCATCTTTCCCGAAAAATCCACCGGAATACCCCCCAGCCCGAGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAg                                                                        <  2:475600/140‑1 (MQ=255)
                                                                                  ccccTGAACCGGTGCGGTGGCGTTGGCGCGCTCCTTCTGTTCCGCAAATCCCCCGGGAAAACCCCCCAGCCCGCAATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAAcc                                                                <  1:574739/148‑1 (MQ=255)
                                                                                   aCCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCgg                                                              >  2:283795/1‑151 (MQ=255)
                                                                                    cctaattggttgcggtgGCGTGGGCGCGCTCCATCTGTTCCGAAAAACCCACCGAAATACCCCCCAGCCCGCAATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGa                                                                   <  2:1218049/135‑1 (MQ=255)
                                                                                     cTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGAGAACCGGt                                                             >  1:1094545/1‑150 (MQ=255)
                                                                                        aaTCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATg                                                         >  2:865455/1‑151 (MQ=255)
                                                                                                  gtggcggtggcgCGCTCCATTTGTCCCGCAAACTCCCCCGAAATACCCCCCAGCCCGCTATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGa                                               <  1:381979/151‑1 (MQ=255)
                                                                                                          ggCGCGCTCCTTCTGTCCCCAAAAACCCCCGGAAAAACCCCCCAGCCCGGAATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAAt                                            <  1:665244/146‑1 (MQ=255)
                                                                                                           gcgcgcTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAgcg                                      >  1:1157747/1‑151 (MQ=255)
                                                                                                                 tCCATCTGTTCCGCAAAATCCATCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCtt                                >  1:737075/1‑151 (MQ=255)
                                                                                                                      cTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGCGAAATCCAGCGCCGCTTCGc                             >  2:648177/1‑149 (MQ=255)
                                                                                                                       tGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCGCTAc                          >  2:53911/1‑151 (MQ=255)
                                                                                                                           ccccaaacccccccGAAATACCCCCCGGCCCGGGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCg                              <  2:268908/131‑1 (MQ=255)
                                                                                                                                aaaCCCACGGGAAAACCCACCCCCCCGGGAATTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCGCTACGCTGTTCCa                 <  1:664647/151‑1 (MQ=255)
                                                                                                                                    cccccGAAATAACCCCCGGCCCGGGAATTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCGCTACGCTGTTCCAGt               <  1:421230/146‑1 (MQ=255)
                                                                                                                                         ggAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCGCTACGCTGTTCCAGTAAAATcc        >  2:337916/1‑151 (MQ=255)
                                                                                                                                         gaaaTATCCACCCGACCGGTAATTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCGCTACGCTGTTCCAGTaaaa           <  1:207020/146‑1 (MQ=255)
                                                                                                                                          gAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCGCTACGCTGTTCCAGTAAAATccc       >  2:807096/1‑151 (MQ=255)
                                                                                                                                           aaaaaCCCCCAGCCCGCAATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCGCTACGCTGTTCCAGTAAAATCCCg      <  2:45282/148‑1 (MQ=255)
                                                                                                                                               accaccAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCGCTACGCTGTTCCAGTAAAATCCCGAccc  >  1:405914/1‑151 (MQ=255)
                                                                                                                                               accaccAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCGCTACGCTGTTCCAGTAAAATCCCGAccc  >  2:70338/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCTGCATTTGCCAGATCTGCCAGATCCCTTCGACATACCCCTGTACAAAGTTAGCGGTATTCGGCTCACTGCCGTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTGGGTGAAATCCAGCGCCGCTTCGCTACGCTGTTCCAGTAAAATCCCGACCC  >  NC_000913/826796‑827089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: