Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,547,7720TG43.8% 142.0 / 19.2 89V367G (GTA→GGA) murPN‑acetylmuramic acid permease, EIIBC component, PTS system
Reads supporting (aligned to +/- strand):  ref base T (14/36);  new base G (39/0);  total (53/36)
Fisher's exact test for biased strand distribution p-value = 9.66e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATTCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTG  >  NC_000913/2547628‑2547920
                                                                                                                                                |                                                                                                                                                    
ttATTCCTGTTTACCTCGCGTTAACGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTAcgcg                                                                                                                                                 <  1:1120903/150‑1 (MQ=255)
 tATTCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCgg                                                                                                                                                <  1:1398826/150‑1 (MQ=255)
   ttCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGGGCAACCGCCCAGGGCGCTACGCAGTCAGGGACGCgggg                                                                                                                                              >  2:1230556/1‑150 (MQ=255)
    tCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCCACGCAGTCAGGTACGCGGGGc                                                                                                                                             <  1:497280/150‑1 (MQ=255)
      cTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCCCAAAGCGCGACGCAGTGAGGGACGCgggggg                                                                                                                                            >  1:1235276/1‑147 (MQ=255)
        gTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCCCACAGTGCGCCACCCCGGCAGGGGCGCggggggg                                                                                                                                           >  1:169906/1‑145 (MQ=255)
        gTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAAACGCCCCGTGGGCCACGCAGGCAGGGACGCgggggggtt                                                                                                                                         >  2:914719/1‑145 (MQ=255)
          ttACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGattat                                                                                                                                       <  1:1292651/150‑1 (MQ=255)
            aCCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTc                                                                                                                                     <  2:525635/150‑1 (MQ=255)
            aCCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTc                                                                                                                                     <  1:1445814/150‑1 (MQ=255)
             ccTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTccc                                                                                                                                   <  1:420151/151‑1 (MQ=255)
             ccTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTccc                                                                                                                                   <  1:853527/151‑1 (MQ=255)
             ccTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCCCCCACTGCGCTACCCCGTCCGGGACGCcgggggggttttttc                                                                                                                                   >  1:1163004/1‑140 (MQ=255)
              cTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCCCACAGGGGGCCACGCAGGCAGGGACGCGGGGCGGGTATTtcc                                                                                                                                   >  1:1280777/1‑150 (MQ=255)
                 gcgTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGcc                                                                                                                               <  1:81449/151‑1 (MQ=255)
                   gTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAAACCCACAGAGCGCCACGCAGCCAGGGTCCCGGGGCGGTTATTtcc                                                                                                                                   >  1:1047041/1‑145 (MQ=255)
                     tAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCCACGCCGTCAGGTACGCGGGGGGGTTTTTTCCCGccccc                                                                                                                            >  2:231245/1‑147 (MQ=255)
                       aTGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGGGCAACCCCACAGTGCGCCACGCCGGCAGGGACGCGGGGGGGTTATTTCCCGCCTGCTgg                                                                                                                         >  2:1017982/1‑151 (MQ=255)
                       aTGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTgg                                                                                                                         <  1:1326955/151‑1 (MQ=255)
                       aTGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCACAGCCCCCCAATGCGCCCCGCCGTGAGGGGAGCGGGGTGAGTATTACCCGccacgccg                                                                                                                         >  1:528471/1‑145 (MQ=255)
                         ggACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGc                                                                                                                       <  2:903773/151‑1 (MQ=255)
                             aGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTg                                                                                                                   <  1:233370/151‑1 (MQ=255)
                               ccAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGGCACCGCCCAAAGCGCTACGCAGTCCGGGACGCGGGGGGGTTATTCCccgcccgcctgggggtgg                                                                                                                  >  2:982436/1‑140 (MQ=255)
                                cAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTg                                                                                                                <  2:1172290/151‑1 (MQ=255)
                                cAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCCCCAACGCACAGGGGGCCACCCGGTGAGGGCCACGGGGGGGTTTTTTCCGGCCTTCCGGGCGtgtggg                                                                                                                >  2:302718/1‑146 (MQ=255)
                                    gATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGGGCCACCGCACAGGGCGCTACCCAGGCAGGGACGCGGGGGGGTTTTTTCCCGCCCGCTGGGGGGTGGGGGAcc                                                                                                            >  1:1072979/1‑151 (MQ=255)
                                    gATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCGCACAGGGCGCTACGCCGTCAGGGACGCGGGGGGGTTATTTCCGGCCCGCTGGGCGGTGGGGaa                                                                                                              >  2:815222/1‑149 (MQ=255)
                                     aTTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCCCCCCGTGCGCCACGCCGGCCGGGACGCGGGGCGGTTATTTCCCGCCCGCTGGGGGGTgggggaac                                                                                                            >  1:726155/1‑144 (MQ=255)
                                     aTTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCCCACCGGGCGCTACGCCGCCAGGTGCGCCGGGCGGATATTCCCGGCCCTCTGGGCGTTGGTGAAcc                                                                                                            >  2:45603/1‑150 (MQ=255)
                                        cAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCt                                                                                                         <  2:102695/150‑1 (MQ=255)
                                         aaCAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGGACGCGGGGCGGTTATTTCCCGCCTGCTGGGCGGTGGTGGACCGc                                                                                                          >  2:1187489/1‑148 (MQ=255)
                                         aaCAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCCCCCCAAGCGCCACGCAGGCCGGGACGCGGGGGGATTTTTTCCCGCCTGCCGGGGGTGGGGGTACCGc                                                                                                          >  1:198064/1‑148 (MQ=255)
                                           cAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCGACCCCACAGTGGGCTACGCGGTCAGGGACGCGGGGGGATTATTTCCCGCCCGCTGGGCGTTGGTGAAACGCTGGtt                                                                                                     >  2:454902/1‑151 (MQ=255)
                                            aGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCCCAATGCGCTACGCAGTCAGGTACGCGGGGGGGTTATTTCCGGCCTGCTGGGCGGTGGTGGACCGCTGGttt                                                                                                    >  2:464327/1‑151 (MQ=255)
                                               ttATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCCCAATGCGCTCCCCAGTCAGGGACGCGGGGGGGGTATTTCCGGCCTGCTGGGCGGTGGTGGACCGCTGGttt                                                                                                    >  2:1264213/1‑148 (MQ=255)
                                                tATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCGCCCAGTGCGCCACGCAGGCCGGGACGCGGGGGGGTTATTCCCCGCCCGCCGGGCGGTGGGGGACCCCTGGTTTTCgg                                                                                                >  1:248475/1‑151 (MQ=255)
                                                 aTTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCAACCCACAGGGCGCTACGCCGGTCGGGAAGCGGGGCGGGTTTTTCCCGCCCGCCCGGCGGTGGGGAACCGCTCCTTTTCgg                                                                                                >  1:1357089/1‑150 (MQ=255)
                                                  tttCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCGCCCAGGGCGCGACGCCGCCAGGGACGCGGGGGGGTTATTCCCCGGCCGCTGGGCGTTGGGGGACCGCCGGTTTTCgg                                                                                                >  1:471818/1‑149 (MQ=255)
                                                   ttCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGGGGCACCCCACAATGCGGCCCGCGGGCAGGGGGGCGGGGGGGTTTTTTCCGGCCCGCCGGGGGCTGGGGGACCggcggtttttcggg                                                                                              >  1:312953/1‑137 (MQ=255)
                                                   ttCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCCCACAGTGCGCTACGCCGGCAGGGACGCGGGGCGGTTATTTCCCGCCCGCCGGGCGGTGGGGGAACGCCGATTTTCgggg                                                                                              >  2:487336/1‑148 (MQ=255)
                                                    tCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCCCCCAGGGCGCCACGCCGGCAGGGAAGCGGGGGGGGTATTTCCCGCCCGCTGGGGGGTTGGGGACCGCTGGTTTTCGgtg                                                                                              >  1:718042/1‑149 (MQ=255)
                                                             ttCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAAACCCCCAGTGCGCCACGGCGTCAAGGCCGCCGGGGGGTTATTTCCCGCCCGCCGGGGGGTGGGGGACCGCCGATTTTAGGGGTCTCCCTcccc                                                                                   >  2:807427/1‑151 (MQ=255)
                                                               cAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCCCCCCGCGGGCTACGCCGTCCGGGACGCGGGGGGGTTATTTCCCGCCTGCTGGGGGGTGGGGGACCGCCGGTTTTCGGGGTCACCCCGcccc                                                                                  >  1:1446227/1‑150 (MQ=255)
                                                               cAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGcccc                                                                                  <  2:125937/150‑1 (MQ=255)
                                                               cAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCg                                                                                 <  2:1073008/151‑1 (MQ=255)
                                                                 aTGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGGCCAACCGCACAGGGCGCTACGCAGCCAGGGACGCGGGGCGATTATTTCCCGCCTGCTGGGCGTTGGTGAAACGCCGATTTTCGGGGGCACCCCGcccc                                                                                  >  2:761136/1‑148 (MQ=255)
                                                                    gCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGGGCGCTACGCAGGCAGGGACGCGGGGGGGTTATTTCCGGCCTGCTGGGGGGTGGGGAACCGCTGGTTTTCGGGGTCCCCCCGCCCCGCCTg                                                                             >  1:1178240/1‑150 (MQ=255)
                                                                     cAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCCCAGTGCCCTACGCAGGCAGGGACGCGGGGGGATTATTCCCGGCCCGCTGGGCGTTGGGGAACCGCTGGTTTTCGGTGTCACCCTGCCCCGCATg                                                                             >  2:548430/1‑149 (MQ=255)
                                                                     cAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGGCAGGGACGCGGGGCGATTATTTCCGGCCTGCCGGGCGGTGGGGAACCGCCGATTTTCGGTGTCACCCTGCCCCCCAt                                                                              >  2:25267/1‑148 (MQ=255)
                                                                      aGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCGCCCACCGCGCCACGCAGTCAGGGACGCGGGGGGGGTATTTCCGGCCTGCTGGGGGGTTGGGAAACGCCGGTTTACCGGGTTCCCCTGCCCCGCATGaa                                                                           >  1:260028/1‑150 (MQ=255)
                                                                      aGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGaa                                                                           <  1:806103/150‑1 (MQ=255)
                                                                      aGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAg                                                                          <  1:414481/151‑1 (MQ=255)
                                                                           cgGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGGCAGGGACGCGGGGCGGTTATTTCCGGCCCGCTGGGGGGTGGTGGAACGCTGATTTACGGTGTCACCCCGGCCCCCATGAAGcc                                                                        >  2:768574/1‑148 (MQ=255)
                                                                            gggCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGGACGCGGGGGGGTTATTCCCGGCCCGCTGGGCGTTGGTGGACCGCTGATTTTCGGTGTCACCCTGCCCCGCATGAAGCCGgtt                                                                    >  2:1070590/1‑151 (MQ=255)
                                                                              gCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAAACCCCCAGTGCGCCACGCAGTCAGGGACGCGGGGCGGGTTTTTCCGGCCCCCTGGGCGGTGGGGGACCGCCCGTTTTCGGTGTCACCCCGCCCCCCACGAAGACGgtt                                                                    >  1:317940/1‑149 (MQ=255)
                                                                               ccAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGtt                                                                 <  2:872996/151‑1 (MQ=255)
                                                                                cAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTa                                                                <  2:689621/151‑1 (MQ=255)
                                                                                 aGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGGAACCGCCCCGTGCGCCACGCGGTTAGGGACGCGGGGGGATTATTCCCCGGCTCCTGGGCGGTTGTGGACCCCTTCTTTATGGTGTTACCCCGCCCCGCATGAAGGCGttttt                                                                  >  2:1226563/1‑146 (MQ=255)
                                                                                 aGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAAGGGGCTACGCCGTCAGGGACGCGGGGCGGATATTTCCCGCCTGCTGGGCGGTGGGGGAACGCTGATTTTCGGGGTCACCCCGCCCCCCCTGAAGCCGTTTGtt                                                                 >  1:164140/1‑149 (MQ=255)
                                                                                   gTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCg                                                             <  1:828084/151‑1 (MQ=255)
                                                                                        cgcggcgcTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCCGGGACGCGGGGGGGTTATTTCCCGCCTGCTGGGCGGTGGTGGACCGCCGATTTTCGGTGTCACCCTGCCCCCCCTGAAGCCGCTTGTTTCCGcc                                                           >  2:303163/1‑148 (MQ=255)
                                                                                           ggcgcTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTa                                                     <  2:957005/151‑1 (MQ=255)
                                                                                             cgcTGGCACTCTACTGGCGGGCGCAAACGCACAGTGCGCTACGCAGTCAGGGACGCGGGGCGATTATTTCCCGCCTGCTGGGCGGTGGTGAAACGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAgg                                                   >  1:757913/1‑151 (MQ=255)
                                                                                              gcTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAggc                                                  <  1:666044/151‑1 (MQ=255)
                                                                                              gcTGGCACTCTACTGGCGGGCCCAACCGCACAGTGCGCTACGCCGGCCGGGACGCGGGGGGGTTATTCCCGGCCCGCGGCGCGGTGGGGAAACGCGGATTTTCGGTGTCACCCTGCCCCGCATGAAGCCGGTTTTTTCCGCCTGCTTTggg                                                  >  1:350105/1‑150 (MQ=255)
                                                                                               cTGGCACTCTACTGGCGGGCGCAAACGCACAGTGCGCCACGCAGTCAGGTACGCGGGGCGCTTATTCCCGGCCTGCTGGGCGGTGGGGGACCCCTGGTTTTCGGCGTCACCCCGCCCCCCCTTAAGACGGTTGTTTCCGCCTGTTTTggcg                                                 >  1:227618/1‑151 (MQ=255)
                                                                                                  gCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGgcggcg                                              <  2:742550/151‑1 (MQ=255)
                                                                                                    aCTCTACTGGCGGGCGCAACCGCACAGGGGGCTACGCAGTCAGGTACGCGGGGCGGTTATTTCCGGCCTGCCGGGCGTTGGTGAACCGCTGATTTTCGGTGTCACCCCGCCCCCCCTGAAGCCGTTTTTTACCGCCTGTTTAGgcggcg                                              >  1:433280/1‑149 (MQ=255)
                                                                                                     ctcgccTGGGGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGGGGGGCGATTATTCCCGGCCTACTGGGCGTTGGTAAACCGCTGATTTACGGTGTCCCCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGgcggcgcg                                            <  1:759581/145‑1 (MQ=255)
                                                                                                         acgggcgggcgCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGcggcgg                                        <  1:416379/147‑1 (MQ=255)
                                                                                                         aCTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGcggcgg                                        <  2:722833/150‑1 (MQ=255)
                                                                                                            ggcgggcgCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCggcgggcg                                     <  2:1357409/150‑1 (MQ=255)
                                                                                                            ggcgggcgCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCggcgggc                                      <  2:234289/149‑1 (MQ=255)
                                                                                                             gcgggcgCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGgt                                   <  2:654709/151‑1 (MQ=255)
                                                                                                              cgggcgCAACCGCACAGTGCGCTACGCAGTCAGGTACTCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGAATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGgt                                   <  2:419084/150‑1 (MQ=255)
                                                                                                                     aaCCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTAt                           >  2:714814/1‑151 (MQ=255)
                                                                                                                        cGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCgg                        >  1:1002482/1‑151 (MQ=255)
                                                                                                                                gcgcTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATa                 <  2:123731/150‑1 (MQ=255)
                                                                                                                                   cTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCt             >  1:1445807/1‑151 (MQ=255)
                                                                                                                                   cTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGCGGTTTGTTTATCGGCCTGATAGCCtg            >  2:1332772/1‑151 (MQ=255)
                                                                                                                                    tACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCtg            >  2:1168432/1‑151 (MQ=255)
                                                                                                                                     aCGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCtgg           >  2:473048/1‑151 (MQ=255)
                                                                                                                                       gCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCtggtg         >  1:1364738/1‑151 (MQ=255)
                                                                                                                                         attCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTggg       >  2:452983/3‑151 (MQ=255)
                                                                                                                                         aGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTggg       >  2:1333302/1‑151 (MQ=255)
                                                                                                                                            cAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGt     <  1:1230522/150‑1 (MQ=255)
                                                                                                                                            cAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGAGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTc    <  2:1450831/151‑1 (MQ=255)
                                                                                                                                             aGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCt   <  2:248484/151‑1 (MQ=255)
                                                                                                                                              ggTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTg  <  2:1163037/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TTATTCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTG  >  NC_000913/2547628‑2547920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: