Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	58.0%	‑1.1 / 97.8	81	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (3/31);  new base T (47/0);  total (50/31)
Fisher's exact test for biased strand distribution p-value = 2.58e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-05

ATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206291‑3206584                                                                                                                                                 |                                                                                                                                                        aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAc                                                                                                                                                    >  2:140533/1‑150 (MQ=255)   ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCATACaa                                                                                                                                                  >  1:860925/1‑150 (MQ=255)    tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACaaa                                                                                                                                                 >  2:1246539/1‑150 (MQ=255)      gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                              <  1:414450/151‑1 (MQ=255)       cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACa                                                                                                                                             <  2:1042688/151‑1 (MQ=255)          aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTCGACAaaaaaaaa                                                                                                                                           >  1:795617/1‑150 (MQ=255)          aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAACaaa                                                                                                                                           >  2:91037/1‑150 (MQ=255)               tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGCATTTTTTCTGACAAAAACCAAAAcag                                                                                                                                      >  2:220352/1‑148 (MQ=255)                 ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATgg                                                                                                                                     >  2:1058876/1‑149 (MQ=255)                 ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATgg                                                                                                                                     >  2:615093/1‑149 (MQ=255)                   aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGtt                                                                                                                                 <  2:184269/151‑1 (MQ=255)                        cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAg                                                                                                                                   >  2:563484/1‑144 (MQ=255)                          gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAAAAAAACTGGAGTTGTCatg                                                                                                                           >  1:1439793/1‑150 (MQ=255)                          gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCat                                                                                                                            <  2:77897/149‑1 (MQ=255)                          gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCat                                                                                                                            <  1:404401/149‑1 (MQ=255)                           ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAACTGAGGTGCCatg                                                                                                                           >  2:1218817/1‑149 (MQ=255)                           ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAAAAATAAAAAAACTGGAGTTGGCatg                                                                                                                           >  1:260865/1‑149 (MQ=255)                               ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAtga‑tga                                                                                                                      <  2:868773/149‑1 (MQ=255)                                 cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGTATTTTTTTAAACAAATAAAAAAAATagagatttctttaag                                                                                                                        >  1:663829/1‑135 (MQ=255)                                   cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGA‑TGAGCGa                                                                                                                  >  2:579689/1‑149 (MQ=255)                                      tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATAGAGGTGCCCTGA‑TGAGCGaaa                                                                                                                >  2:630286/1‑148 (MQ=255)                                        ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCTAACAAAAAAAAAAAATGGGGGTGCCAcga‑tgaagggaaaaaa                                                                                                            >  2:221495/1‑140 (MQ=255)                                          cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGtaataa                                                                                                         <  1:786069/151‑1 (MQ=255)                                          cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGtaata                                                                                                          <  1:770638/150‑1 (MQ=255)                                            ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTTTCGCAAAAACAAAAACCAGTGGTGCCatgg‑tgggggca                                                                                                                 >  1:911286/1‑135 (MQ=255)                                            ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAAAAAAAATTGAGGTGCCATGA‑agaggggaaagaaaaa                                                                                                         >  2:624538/1‑137 (MQ=255)                                             tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGc                                                                                                       <  1:228606/150‑1 (MQ=255)                                              cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAAAAAAAAAAATGGAGTTGCCATTA‑AGAGCGGAAAtaaaaa                                                                                                         >  2:628187/1‑147 (MQ=255)                                                tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCaa                                                                                                     <  1:487308/149‑1 (MQ=255)                                                      cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAg                                                                                              <  1:857857/150‑1 (MQ=255)                                                      cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAACAACAGGCAGt                                                                                             <  2:25864/151‑1 (MQ=255)                                                        cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGa                                                                                           <  2:1407977/151‑1 (MQ=255)                                                         gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAAAACAAAAAATGGAGGTGTCATGA‑TGAGCGAAAAGAATAAACAAAAAGCAggg                                                                                            >  1:1012907/1‑147 (MQ=255)                                                           tGATTCACGAACTGTTAATTACATATTCATGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAAACAAAAAAAAAAAATGGAGGTGTCATGA‑TGAGGGAAAGAAATAAAAAAAAGGGAGGGaa                                                                                          >  2:538058/1‑149 (MQ=255)                                                           tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCCGACAAAAACAAAAAATGGAGGTGCCATGA‑TGAGGGAAAGGAAAAAAAAAAAGGCaaggga                                                                                          >  2:1210767/1‑144 (MQ=255)                                                            gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                        <  1:274154/150‑1 (MQ=255)                                                             aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTCTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAAGAGGCAGTGAATaat                                                                                      <  1:930734/151‑2 (MQ=255)                                                             aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAAAAAAAAAAAAGGAGTTTGCCTGA‑TGAGGGAAAAAAAAAAAAAAAAGGCAGGGAaaaa                                                                                       >  1:903129/1‑150 (MQ=255)                                                                cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAAACAAAAAAAAAAAATGGAGGTGTCATTA‑TGAGGGGAAAGAATAAACAAAAAGCAGTGGATAAGtt                                                                                    >  1:757746/1‑150 (MQ=255)                                                                   gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAAAAGAGGTGGCATGG‑TGAGGGAAAAAAAAAAAAAACAAGCAGGGaaaa                                                                                        >  1:904639/1‑141 (MQ=255)                                                                    aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAAAAAAAAAGGAGTTGGCATGA‑TGAGGGGAAGGAAAAAACaaaaaggagggaa                                                                                          >  2:891044/1‑129 (MQ=255)                                                                    aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAAACAAAAAAAAAAAAAGGAGTTGTCATGG‑TGAGGGAAAGGAATAAGAAAAAAGGAGGGAATaa                                                                                       >  1:56999/1‑143 (MQ=255)                                                                     aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAACAAAAATAAAAACTGGAGGTTTCGTGA‑TGGGCGGAAGGGAAAAACAACAGGGAGTGAATAAGTTGACa                                                                                >  1:177489/1‑149 (MQ=255)                                                                        gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                            <  1:478371/150‑1 (MQ=255)                                                                        gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                            <  2:1357346/150‑1 (MQ=255)                                                                        gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                           <  1:728560/151‑1 (MQ=255)                                                                           aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCCTGA‑TGAGGGGAAGGAATAAACAAAAAGGAGGGAATAAGTTTACAgagaat                                                                          >  2:784672/1‑147 (MQ=255)                                                                           aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAAACAAAAAAAAAAAATGGAGGTGCCATGA‑TGAGCGGAAGGAATAAAAAAAAAGCAGAGAAAAAAATGACAgagaat                                                                          >  1:114642/1‑147 (MQ=255)                                                                                 atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTa                                                                  <  1:569132/150‑1 (MQ=255)                                                                                  tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAATGGAGGTGTCATGA‑TGAGCGGAAGAAATAAACAAAAAGGAGGGAAAAAATTGACAGAGATTGTCG‑CTaa                                                                 >  1:1318566/1‑150 (MQ=255)                                                                                    ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATTGAGGTGGCATGA‑TGAGCGAAAGAAATAAACAAAAAGCAGGGAATAAGATGAAAGAGAATGTTG‑CTaa                                                                 >  2:705260/1‑148 (MQ=255)                                                                                      cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTa                                                            <  2:1061378/151‑1 (MQ=255)                                                                                       aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAAACAAAAAATGGAGGTTCCATGA‑TGAGCGGAAGTAATAAACAAAAAGCCGGGAATAAATTGACAGAGATTTTTG‑CTAAAttt                                                             >  1:858459/1‑149 (MQ=255)                                                                                        cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAAAAAAAATGGAGGTGGCATGA‑TGAGGGAAAGAAAAAAACAAAAAGCAGGGAATAAGTTGACAAAGAATGGCG‑GTaaaattaac                                                          >  2:1035029/1‑144 (MQ=255)                                                                                             gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTTTCCATGA‑TGAGCGAAAGTAATAAACAACAAGCAGTGAATAAATTGACAGAGATTGTCG‑CTAACTTTACcccc                                                       >  2:77511/1‑149 (MQ=255)                                                                                                 aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAACAAAAAATGGAGGTTGCATGA‑TGAGCGAAAGTAATAAAAAAAAAGCAGAGAAAAAATTGACAAAGAATGTTG‑CTCAATTTTCCCCCCTGAtt                                                 >  1:703826/1‑151 (MQ=255)                                                                                                    atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAAAAAAAAAAAATGGAGGTGGCATGG‑TGGGGGAAAGTAATAAAAAAAAAAAAGGGAAAAAAGTGAAAGAGAATGTTG‑CTAAATTTAACCCCCTGAttttt                                              >  1:252587/1‑149 (MQ=255)                                                                                                      ataAAACCACACTTTTAGTGGTAGTTTGTGGCGGCCAATTTTTTCACACAACAACAAAAAATGGAGTTGCCATAG‑TGAGCGAAAGTAATAAACCAAAAGGAGGGAATAAAATGGGAAAGAAAGTCG‑CTAAACTTAcacatctgattttt                                              >  1:1084147/1‑136 (MQ=255)                                                                                                       taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAACTTGAGTTGGCATGG‑TGAGCGGAAGAAAAAAAAAACAAGCAGGGGATAAAATGACAGAGAATTTCG‑CTAACTTTTCCCCCCTGATTTTTAcc                                           >  2:1301092/1‑151 (MQ=255)                                                                                                        aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAAATGGAGGTGGCCTGA‑TGAGGGAAAGGAATAAACAAAAAGCAGGGAATAAAATGACAGAGATTGTTGCCTAATTTTA‑CGCCCTGATTTTTAccc                                          >  1:848583/1‑151 (MQ=255)                                                                                                           aCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACCAAAAAAAAAAATGGAGTTTGCATGAGTGGGGGAAAG‑AATAAACAAAAAGCAGGGGAAAAGTTGACAGAGAATGTCG‑CTAAATTTTACCCCCTGATTTTTAcccc                                         >  1:393528/1‑148 (MQ=255)                                                                                                            ccACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGACAAAAACAAAAAATTGAGGTGGCCTGA‑TGAGCGGAAGGAAAAAAAAAAAAGCAGGGAATAAATTTACAGAGATTTTCG‑CTAAATTTTCCGCCCTTATTTTTACCCCaa                                       >  2:1330775/1‑150 (MQ=255)                                                                                                             cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGA‑TGAGCGGAAGTAAAAAACAACAAGCAGGGAATAAGGTGACAGAGATTGTTG‑CTAACTTTACCGCCATGATTTTTTccc                                          >  2:1193766/1‑146 (MQ=255)                                                                                                               caTTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGGCAAATACAAAAAATTGAGGTGGCCTGG‑TGAGCGGAAAAAATAAAGAACAAGGCGGGAATAAGGTGAGAGAGGATTTCG‑CTAACTTTACCACCCTTATTTTTACACCAATGcc                                   >  2:750702/1‑151 (MQ=255)                                                                                                                  ttttAGTGGTAGTTTGTGGGGGTAAATTTTTCCAGAAAAATAAAAAAACGGGAGTTGCCATGA‑TGAGCAAAAGAAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCtg                                 <  1:772201/150‑1 (MQ=255)                                                                                                                  ttttAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAAATACAAAAAAAGGGGGTGGCATGG‑TGAGGGAAAGAAATAAAAAACAGGGAGGGAAAAAAATGAAAAAGAATTTCG‑CTAAACTTACCGCCCTGATTTTTACCCGAAAGcc                                   >  1:1197678/1‑148 (MQ=255)                                                                                                                  ttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCACCCAAATAAAAAAAATCGGGGTTCCCTGG‑TGAGGGGAAATAATAAACAAAAAGCAGAGAAAAAGTTGACAGAGATTGTCG‑CTAAATTTACCGCCCTGATTTTTTCCCGAAAGCCtg                                 >  1:759093/1‑150 (MQ=255)                                                                                                                      aGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGAGGTGCCATGA‑TGAGCGAAAGTAATAAACAACAAGCAGTGAATAAGATGACAGAGATTTTCG‑CTAACTTTACCGCCCTGATTTCTACCCGAATGCCtgat                               >  1:1001308/1‑148 (MQ=255)                                                                                                                         ggTAGTTTGTGGGGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                         <  1:642604/151‑1 (MQ=255)                                                                                                                         ggTAGTTTGTGGGGGTAAATTTTCCCAGAAAAATACAAAAACTGGAGTTGCCATGA‑GGGGGGAATTTAATAAGCAACGGGCAGTGAATAAGTTTACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                          <  2:997212/150‑1 (MQ=255)                                                                                                                              tttGTGGCGGTGAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATTA‑TGAGCGAAAGAAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCG‑CTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg                      <  2:833697/149‑1 (MQ=255)                                                                                                                              tttGTGGCGGTGAATTTTTCCAGAAAAATAAAAAAACGGGAGTTGCCATGA‑TGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                     <  2:277298/150‑1 (MQ=255)                                                                                                                               ttGGGGGGGTGAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                    <  1:1054664/150‑1 (MQ=255)                                                                                                                                 gtgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAATTAATAAGCAACAGGCAGTGAATAATTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                 <  1:1440427/151‑1 (MQ=255)                                                                                                                                 gtgGCGGTGAATTTTTCCAGAAAAAAAAAAAAACTGGAGTTGCCATGA‑TGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTAAAAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa                  <  1:977644/150‑1 (MQ=255)                                                                                                                                 gtgGCGGTAAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa                  <  2:1166241/150‑1 (MQ=255)                                                                                                                                 ggggCGGTGAATTTTTCAAGACAAATAAAAAAACGGGAGTTGCCATAA‑TGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAATTGGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa                  <  2:87994/148‑1 (MQ=255)                                                                                                                                   ggCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCAAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCG‑CAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa                <  2:1265027/150‑1 (MQ=255)                                                                                                                                     cGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt              >  2:1275840/1‑150 (MQ=255)                                                                                                                                        tGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa           >  2:1246059/1‑150 (MQ=255)                                                                                                                                            tttttCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc      >  2:1329858/1‑151 (MQ=255)                                                                                                                                                 ccAGACAAATAAAAAAACGGGAGTTGCCATGA‑TGAGGGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGCCG‑CTACCTTTACCGCCATGATTTCTACTCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa  <  2:186668/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                        ATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206291‑3206584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑