Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,452	0	C	A	42.9%	105.2 / 49.8	84	intergenic (‑196/‑11)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (14/34);  new base A (36/0);  total (50/34)
Fisher's exact test for biased strand distribution p-value = 2.35e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGT  >  NC_000913/3206305‑3206601                                                                                                                                                    |                                                                                                                                                        tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGCATTTTTTCTGACAAAAACCAAAAcag                                                                                                                                                       >  2:220352/1‑148 (MQ=255)   ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATgg                                                                                                                                                      >  2:1058876/1‑149 (MQ=255)   ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATgg                                                                                                                                                      >  2:615093/1‑149 (MQ=255)     aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGtt                                                                                                                                                  <  2:184269/151‑1 (MQ=255)          cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAg                                                                                                                                                    >  2:563484/1‑144 (MQ=255)            gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAAAAAAACTGGAGTTGTCatg                                                                                                                                            >  1:1439793/1‑150 (MQ=255)            gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCat                                                                                                                                             <  1:404401/149‑1 (MQ=255)            gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCat                                                                                                                                             <  2:77897/149‑1 (MQ=255)             ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAACTGAGGTGCCatg                                                                                                                                            >  2:1218817/1‑149 (MQ=255)             ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAAAAATAAAAAAACTGGAGTTGGCatg                                                                                                                                            >  1:260865/1‑149 (MQ=255)                 ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAtga‑tga                                                                                                                                       <  2:868773/149‑1 (MQ=255)                   cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGTATTTTTTTAAACAAATAAAAAAAATagagatttctttaag                                                                                                                                         >  1:663829/1‑135 (MQ=255)                     cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGA‑TGAGCGa                                                                                                                                   >  2:579689/1‑149 (MQ=255)                        tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATAGAGGTGCCCTGA‑TGAGCGaaa                                                                                                                                 >  2:630286/1‑148 (MQ=255)                          ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCTAACAAAAAAAAAAAATGGGGGTGCCAcga‑tgaagggaaaaaa                                                                                                                             >  2:221495/1‑140 (MQ=255)                            cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGtaataa                                                                                                                          <  1:786069/151‑1 (MQ=255)                            cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGtaata                                                                                                                           <  1:770638/150‑1 (MQ=255)                              ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTTTCGCAAAAACAAAAACCAGTGGTGCCatgg‑tgggggca                                                                                                                                  >  1:911286/1‑135 (MQ=255)                              ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAAAAAAAATTGAGGTGCCATGA‑agaggggaaagaaaaa                                                                                                                          >  2:624538/1‑137 (MQ=255)                               tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGc                                                                                                                        <  1:228606/150‑1 (MQ=255)                                cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAAAAAAAAAAATGGAGTTGCCATTA‑AGAGCGGAAAtaaaaa                                                                                                                          >  2:628187/1‑147 (MQ=255)                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCaa                                                                                                                      <  1:487308/149‑1 (MQ=255)                                        cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAg                                                                                                               <  1:857857/150‑1 (MQ=255)                                        cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAACAACAGGCAGt                                                                                                              <  2:25864/151‑1 (MQ=255)                                          cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGa                                                                                                            <  2:1407977/151‑1 (MQ=255)                                           gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAAAACAAAAAATGGAGGTGTCATGA‑TGAGCGAAAAGAATAAACAAAAAGCAggg                                                                                                             >  1:1012907/1‑147 (MQ=255)                                             tGATTCACGAACTGTTAATTACATATTCATGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAAACAAAAAAAAAAAATGGAGGTGTCATGA‑TGAGGGAAAGAAATAAAAAAAAGGGAGGGaa                                                                                                           >  2:538058/1‑149 (MQ=255)                                             tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCCGACAAAAACAAAAAATGGAGGTGCCATGA‑TGAGGGAAAGGAAAAAAAAAAAGGCaaggga                                                                                                           >  2:1210767/1‑144 (MQ=255)                                              gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                                         <  1:274154/150‑1 (MQ=255)                                               aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTCTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAAGAGGCAGTGAATaat                                                                                                       <  1:930734/151‑2 (MQ=255)                                               aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAAAAAAAAAAAAGGAGTTTGCCTGA‑TGAGGGAAAAAAAAAAAAAAAAGGCAGGGAaaaa                                                                                                        >  1:903129/1‑150 (MQ=255)                                                  cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAAACAAAAAAAAAAAATGGAGGTGTCATTA‑TGAGGGGAAAGAATAAACAAAAAGCAGTGGATAAGtt                                                                                                     >  1:757746/1‑150 (MQ=255)                                                     gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAAAAGAGGTGGCATGG‑TGAGGGAAAAAAAAAAAAAACAAGCAGGGaaaa                                                                                                         >  1:904639/1‑141 (MQ=255)                                                      aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAAAAAAAAAGGAGTTGGCATGA‑TGAGGGGAAGGAAAAAACaaaaaggagggaa                                                                                                           >  2:891044/1‑129 (MQ=255)                                                      aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAAACAAAAAAAAAAAAAGGAGTTGTCATGG‑TGAGGGAAAGGAATAAGAAAAAAGGAGGGAATaa                                                                                                        >  1:56999/1‑143 (MQ=255)                                                       aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAACAAAAATAAAAACTGGAGGTTTCGTGA‑TGGGCGGAAGGGAAAAACAACAGGGAGTGAATAAGTTGACa                                                                                                 >  1:177489/1‑149 (MQ=255)                                                          gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                             <  1:478371/150‑1 (MQ=255)                                                          gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                             <  2:1357346/150‑1 (MQ=255)                                                          gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                            <  1:728560/151‑1 (MQ=255)                                                             aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCCTGA‑TGAGGGGAAGGAATAAACAAAAAGGAGGGAATAAGTTTACAgagaat                                                                                           >  2:784672/1‑147 (MQ=255)                                                             aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAAACAAAAAAAAAAAATGGAGGTGCCATGA‑TGAGCGGAAGGAATAAAAAAAAAGCAGAGAAAAAAATGACAgagaat                                                                                           >  1:114642/1‑147 (MQ=255)                                                                   atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTa                                                                                   <  1:569132/150‑1 (MQ=255)                                                                    tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAATGGAGGTGTCATGA‑TGAGCGGAAGAAATAAACAAAAAGGAGGGAAAAAATTGACAGAGATTGTCG‑CTaa                                                                                  >  1:1318566/1‑150 (MQ=255)                                                                      ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATTGAGGTGGCATGA‑TGAGCGAAAGAAATAAACAAAAAGCAGGGAATAAGATGAAAGAGAATGTTG‑CTaa                                                                                  >  2:705260/1‑148 (MQ=255)                                                                        cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTa                                                                             <  2:1061378/151‑1 (MQ=255)                                                                         aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAAACAAAAAATGGAGGTTCCATGA‑TGAGCGGAAGTAATAAACAAAAAGCCGGGAATAAATTGACAGAGATTTTTG‑CTAAAttt                                                                              >  1:858459/1‑149 (MQ=255)                                                                          cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAAAAAAAATGGAGGTGGCATGA‑TGAGGGAAAGAAAAAAACAAAAAGCAGGGAATAAGTTGACAAAGAATGGCG‑GTaaaattaac                                                                           >  2:1035029/1‑144 (MQ=255)                                                                               gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTTTCCATGA‑TGAGCGAAAGTAATAAACAACAAGCAGTGAATAAATTGACAGAGATTGTCG‑CTAACTTTACcccc                                                                        >  2:77511/1‑149 (MQ=255)                                                                                   aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAACAAAAAATGGAGGTTGCATGA‑TGAGCGAAAGTAATAAAAAAAAAGCAGAGAAAAAATTGACAAAGAATGTTG‑CTCAATTTTCCCCCCTGAtt                                                                  >  1:703826/1‑151 (MQ=255)                                                                                      atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAAAAAAAAAAAATGGAGGTGGCATGG‑TGGGGGAAAGTAATAAAAAAAAAAAAGGGAAAAAAGTGAAAGAGAATGTTG‑CTAAATTTAACCCCCTGAttttt                                                               >  1:252587/1‑149 (MQ=255)                                                                                        ataAAACCACACTTTTAGTGGTAGTTTGTGGCGGCCAATTTTTTCACACAACAACAAAAAATGGAGTTGCCATAG‑TGAGCGAAAGTAATAAACCAAAAGGAGGGAATAAAATGGGAAAGAAAGTCG‑CTAAACTTAcacatctgattttt                                                               >  1:1084147/1‑136 (MQ=255)                                                                                         taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAACTTGAGTTGGCATGG‑TGAGCGGAAGAAAAAAAAAACAAGCAGGGGATAAAATGACAGAGAATTTCG‑CTAACTTTTCCCCCCTGATTTTTAcc                                                            >  2:1301092/1‑151 (MQ=255)                                                                                          aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAAATGGAGGTGGCCTGA‑TGAGGGAAAGGAATAAACAAAAAGCAGGGAATAAAATGACAGAGATTGTTGCCTAATTTTA‑CGCCCTGATTTTTAccc                                                           >  1:848583/1‑151 (MQ=255)                                                                                             aCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACCAAAAAAAAAAATGGAGTTTGCATGAGTGGGGGAAAG‑AATAAACAAAAAGCAGGGGAAAAGTTGACAGAGAATGTCG‑CTAAATTTTACCCCCTGATTTTTAcccc                                                          >  1:393528/1‑148 (MQ=255)                                                                                              ccACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGACAAAAACAAAAAATTGAGGTGGCCTGA‑TGAGCGGAAGGAAAAAAAAAAAAGCAGGGAATAAATTTACAGAGATTTTCG‑CTAAATTTTCCGCCCTTATTTTTACCCCaa                                                        >  2:1330775/1‑150 (MQ=255)                                                                                               cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGA‑TGAGCGGAAGTAAAAAACAACAAGCAGGGAATAAGGTGACAGAGATTGTTG‑CTAACTTTACCGCCATGATTTTTTccc                                                           >  2:1193766/1‑146 (MQ=255)                                                                                                 caTTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGGCAAATACAAAAAATTGAGGTGGCCTGG‑TGAGCGGAAAAAATAAAGAACAAGGCGGGAATAAGGTGAGAGAGGATTTCG‑CTAACTTTACCACCCTTATTTTTACACCAATGcc                                                    >  2:750702/1‑151 (MQ=255)                                                                                                    ttttAGTGGTAGTTTGTGGGGGTAAATTTTTCCAGAAAAATAAAAAAACGGGAGTTGCCATGA‑TGAGCAAAAGAAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCtg                                                  <  1:772201/150‑1 (MQ=255)                                                                                                    ttttAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAAATACAAAAAAAGGGGGTGGCATGG‑TGAGGGAAAGAAATAAAAAACAGGGAGGGAAAAAAATGAAAAAGAATTTCG‑CTAAACTTACCGCCCTGATTTTTACCCGAAAGcc                                                    >  1:1197678/1‑148 (MQ=255)                                                                                                    ttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCACCCAAATAAAAAAAATCGGGGTTCCCTGG‑TGAGGGGAAATAATAAACAAAAAGCAGAGAAAAAGTTGACAGAGATTGTCG‑CTAAATTTACCGCCCTGATTTTTTCCCGAAAGCCtg                                                  >  1:759093/1‑150 (MQ=255)                                                                                                        aGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGAGGTGCCATGA‑TGAGCGAAAGTAATAAACAACAAGCAGTGAATAAGATGACAGAGATTTTCG‑CTAACTTTACCGCCCTGATTTCTACCCGAATGCCtgat                                                >  1:1001308/1‑148 (MQ=255)                                                                                                           ggTAGTTTGTGGGGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                          <  1:642604/151‑1 (MQ=255)                                                                                                           ggTAGTTTGTGGGGGTAAATTTTCCCAGAAAAATACAAAAACTGGAGTTGCCATGA‑GGGGGGAATTTAATAAGCAACGGGCAGTGAATAAGTTTACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                                           <  2:997212/150‑1 (MQ=255)                                                                                                                tttGTGGCGGTGAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATTA‑TGAGCGAAAGAAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCG‑CTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg                                       <  2:833697/149‑1 (MQ=255)                                                                                                                tttGTGGCGGTGAATTTTTCCAGAAAAATAAAAAAACGGGAGTTGCCATGA‑TGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                                      <  2:277298/150‑1 (MQ=255)                                                                                                                 ttGGGGGGGTGAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                     <  1:1054664/150‑1 (MQ=255)                                                                                                                   gtgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAATTAATAAGCAACAGGCAGTGAATAATTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                                  <  1:1440427/151‑1 (MQ=255)                                                                                                                   gtgGCGGTGAATTTTTCCAGAAAAAAAAAAAAACTGGAGTTGCCATGA‑TGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTAAAAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa                                   <  1:977644/150‑1 (MQ=255)                                                                                                                   gtgGCGGTAAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa                                   <  2:1166241/150‑1 (MQ=255)                                                                                                                   ggggCGGTGAATTTTTCAAGACAAATAAAAAAACGGGAGTTGCCATAA‑TGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAATTGGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa                                   <  2:87994/148‑1 (MQ=255)                                                                                                                     ggCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCAAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCG‑CAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa                                 <  2:1265027/150‑1 (MQ=255)                                                                                                                       cGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt                               >  2:1275840/1‑150 (MQ=255)                                                                                                                          tGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                            >  2:1246059/1‑150 (MQ=255)                                                                                                                              tttttCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc                       >  2:1329858/1‑151 (MQ=255)                                                                                                                                   ccAGACAAATAAAAAAACGGGAGTTGCCATGA‑TGAGGGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGCCG‑CTACCTTTACCGCCATGATTTCTACTCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa                   <  2:186668/150‑1 (MQ=255)                                                                                                                                     aGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAgg                 <  2:847683/150‑1 (MQ=255)                                                                                                                                       aaaaaTACAAAAACTGGAGTTGCCATGA‑TGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATg              <  1:629594/149‑1 (MQ=255)                                                                                                                                       aCAAATACAAAAACTGGAGTTGCCATGA‑TGAGCAAAAGTAATAAGAAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAgg                 <  1:1360175/148‑1 (MQ=255)                                                                                                                                        cAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGc             >  2:442488/1‑151 (MQ=255)                                                                                                                                         aaaTAAAAAAACTGGAGTTGCCATGA‑TGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGcc            <  2:320722/151‑1 (MQ=255)                                                                                                                                          aaTACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCg           >  2:1284604/1‑151 (MQ=255)                                                                                                                                              cAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAc       >  2:453772/1‑151 (MQ=255)                                                                                                                                                 aatcTGGAGTGGCCATGA‑TGAGCGAAATTAATAAGCAACAGGCAGTAAATAAGTTGACAGATTTTGTCC‑TTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtc    <  2:208558/148‑1 (MQ=255)                                                                                                                                                  aaCTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcg   >  1:951348/1‑151 (MQ=255)                                                                                                                                                  aaCTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcg   >  1:905806/1‑151 (MQ=255)                                                                                                                                                  aaCTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcg   >  2:472870/1‑151 (MQ=255)                                                                                                                                                    cTGGAGTTGCCATGA‑TGAGCGAAAGAAATAAGCAACAGGCAGTAAAAAAGTTAACAGAGATTGTCG‑CTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcgt  <  2:369818/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                        TGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCG‑CTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGT  >  NC_000913/3206305‑3206601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑