Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,282	0	T	G	44.9%	114.6 / 15.8	78	V526G (GTT→GGT)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (10/33);  new base G (35/0);  total (45/33)
Fisher's exact test for biased strand distribution p-value = 1.80e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCG  >  NC_000913/354138‑354429                                                                                                                                                 |                                                                                                                                                    aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTggggg                                                                                                                                               >  2:606325/1‑150 (MQ=255)    ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTggggggg                                                                                                                                             >  2:1405431/1‑147 (MQ=255)     gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaaa                                                                                                                                           <  1:980665/151‑1 (MQ=255)       gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTgggggg                                                                                                                                              >  1:1029179/1‑144 (MQ=255)          tCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATg                                                                                                                                       >  2:1105510/1‑150 (MQ=255)           ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcg                                                                                                                                     <  2:847222/151‑1 (MQ=255)           ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATgcg                                                                                                                                     >  2:672214/1‑151 (MQ=255)              tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATgcg                                                                                                                                     >  2:706953/1‑148 (MQ=255)               gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGGCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAgcgc                                                                                                                                    >  1:751794/1‑148 (MQ=255)               gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTg                                                                                                                                  <  1:201104/150‑1 (MQ=255)               gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGa                                                                                                                                 <  1:965888/151‑1 (MQ=255)               gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGGAAGAGgcgc                                                                                                                                    >  1:762619/1‑148 (MQ=255)                aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                <  2:986037/151‑1 (MQ=255)                aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                <  2:1354686/151‑1 (MQ=255)                 cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTggggggggggggagaagcggcg                                                                                                                                  >  1:589184/1‑134 (MQ=255)                     gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGTGGTGGTTGGGGTGAAAGATGCGCTGAAAgg                                                                                                                             <  1:890890/149‑1 (MQ=255)                      aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAggg                                                                                                                            >  2:409898/1‑149 (MQ=255)                        ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCCGGAAggggcgg                                                                                                                        >  1:563125/1‑147 (MQ=255)                         gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggt                                                                                                                       <  1:639885/151‑1 (MQ=255)                         gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGAAAGGGGAgg                                                                                                                        >  1:862911/1‑150 (MQ=255)                           tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGAAAGGGGAggggg                                                                                                                     >  2:882364/1‑151 (MQ=255)                                   tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAgggggggggggggggg                                                                                                               >  2:948511/1‑136 (MQ=255)                                   tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGGAAGGGGAgggggcggggggg                                                                                                             >  1:1351352/1‑149 (MQ=255)                                    aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGt                                                                                                            <  1:1393168/151‑1 (MQ=255)                                       ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAAATGCGCGGGAACGGGAgggggcggggggggtt                                                                                                          >  1:685354/1‑145 (MQ=255)                                         cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGttt                                                                                                          <  1:338837/148‑1 (MQ=255)                                         cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTg                                                                                                         <  1:821118/149‑1 (MQ=255)                                         cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGGAAGGGGAGGTGGCGGGGGCGgtt                                                                                                          >  2:780918/1‑148 (MQ=255)                                            gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCAtt                                                                                                    <  2:705419/151‑1 (MQ=255)                                               cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAATGCGCTGGAAAGGGAGGGGGGGGGGGGGGTTGTTAtttc                                                                                                  >  2:181903/1‑148 (MQ=255)                                               cACTGACGATGTAATTAACGGTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGGTGGAAGGGGAGGGGGGGGGGGGGGTTggtctttc                                                                                                  >  1:635441/1‑143 (MQ=255)                                                aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGGGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTCCtttc                                                                                                  >  1:709591/1‑147 (MQ=255)                                                      gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagaga                                                                                          <  2:172572/151‑1 (MQ=255)                                                       aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagagag                                                                                         <  1:274004/151‑1 (MQ=255)                                                       aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTGtggtttcggaa                                                                                              >  1:664657/1‑136 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGCGGTTGTCATTCCGGAaga                                                                                            >  2:552048/1‑146 (MQ=255)                                                           aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGa                                                                                      <  2:564485/150‑1 (MQ=255)                                                           aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAGGCGCTGGAAGGGGAGGTGGCGGGGGGGGTTGTCATTCCGGAagaga                                                                                          >  1:864828/1‑146 (MQ=255)                                                             ttAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                    <  2:943591/150‑1 (MQ=255)                                                                    tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCCGGAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGGAAGAGAGCGGAAGTCTgg                                                                              >  1:96407/1‑149 (MQ=255)                                                                     gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                            <  2:1053999/150‑1 (MQ=255)                                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGGTTTTCCTTTCGAAAGAGAGCGACAGTc                                                                                 >  1:250465/1‑144 (MQ=255)                                                                       cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                         <  2:60559/151‑1 (MQ=255)                                                                       cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGCGCCGAAAAGGCAGGTGGGGGGGGGGGTTGTCATTTCCAAAAAGAGCGGAAGTCTGGaa                                                                            >  1:1263434/1‑148 (MQ=255)                                                                         ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCg                                                                       <  2:655511/151‑1 (MQ=255)                                                                          gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGt                                                                      <  1:1197649/151‑1 (MQ=255)                                                                                gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                <  1:1418309/151‑1 (MQ=255)                                                                                gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCggg                                                                     >  2:90905/1‑144 (MQ=255)                                                                                   ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgc                                                              <  2:475946/150‑1 (MQ=255)                                                                                   ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGAAAGGGGAGGGGGCGGGGGCGGTTTTTATTTCGAAAGAGAGCGGCAGTCTGGGAGATCGTGGTGTgg                                                               >  1:1417897/1‑149 (MQ=255)                                                                                     ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGCGGGGGGGGGGGGGGTTGTTATTTCGAAAGAGAGGGACAGTCTGGAAGATCGGGATGTGgcg                                                             >  1:956664/1‑149 (MQ=255)                                                                                        cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACta                                                        <  1:1403839/151‑2 (MQ=255)                                                                                        cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                        <  1:122785/151‑1 (MQ=255)                                                                                        cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                        <  2:267118/151‑1 (MQ=255)                                                                                          cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGGGCCGAAAAGGGAGGTGGGGGTGGCGGTTGTCATTTCGGAAGAGAGCGACCGTCTGGAAGATAGTGGTGTGGGGCACCCGc                                                      >  1:558330/1‑151 (MQ=255)                                                                                              agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTTTCCTTCCGAAAGAGAGCGACAGTCTGGGAGAACGTGATGTGGGGCACTCGc                                                      >  1:89248/1‑147 (MQ=255)                                                                                               gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAAAGCGCCGTAAAGGGAGGTGGCGGTGGCGTTTGTCATTTCGAAAAAGGGCGCCAGTCTGGAAGATCGCGGAGTGGCGCCCTCGcca                                                    >  1:214820/1‑146 (MQ=255)                                                                                                  tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAAAAGCGCCGAAAGGGGAGGGGGCGGGGGGGGTTGGCATTCCGAAAGAGAGAGACAGTCTGGGAGATAGGGGTGGGGCGCACTCGCAagag                                                 >  1:543218/1‑148 (MQ=255)                                                                                                    aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                            <  2:1230356/151‑1 (MQ=255)                                                                                                     agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                           <  1:253288/151‑1 (MQ=255)                                                                                                          gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                      <  1:455950/151‑1 (MQ=255)                                                                                                           tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAAGGGGGGGGGCGGGGGGGGTTGTCATTCCCCAAGAGAGCGACAGTCTGGGAGGGCGTGATGTGGGGCCCTCTCCAGAGAAAGGGAatat                                      >  2:1418539/1‑150 (MQ=255)                                                                                                           tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGGAGGGGGCGGGGGCGGTTGTTATTCCGAAAGAGAGCGACAGGCTGGAAGATCGGGATGTGGGGCACTCGCCAGAGaaaggggtt                                        >  1:917206/1‑141 (MQ=255)                                                                                                             tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGAAGATGCGCTGAAAGGGCAGGTGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                        >  2:490341/1‑146 (MQ=255)                                                                                                             tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGTTGGGGGGAAAAATGCGCCCGAACGGGAGGGGGGGGGGGGGGTTGTCATTCCGCAAGAGAGCGACAGTCTGGAAGATAGGGGTGTGGCGGACCAGCCAGAGAAAGCg                                           >  1:1148926/1‑143 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgc                                   <  1:423685/148‑1 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGGTTTTGgcg                                  >  2:913854/1‑149 (MQ=255)                                                                                                                  aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAAAACGCCGGAAGGGGCGGGGGGGGGGGGGTTTGTCATTGCGGAAGAGAGGGGCAGTCTGGAAAAACGGGATGGGGGGCCCTCGCCAGAGAAAGCGATTATGGGGCtgg                              >  1:636751/1‑151 (MQ=255)                                                                                                                    ccATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtg                            >  2:513781/1‑151 (MQ=255)                                                                                                                      aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCCGAAAGGGGAGGGGGGGGGGGCGTTTGGTATTCCGAAAGAGAGCGCCAGACTGGGAGATTGTGGTGTGGCGCACTCGCAAGAGAAGGCGGGTATGGCGCtggtg                            >  1:258181/1‑149 (MQ=255)                                                                                                                      aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCCGGAAGGGGCGGGGGCGGGGGGGTTTGTCATTTCGAAAAAGAGCGACAGGCTGGAAGAACGTGGTGTGGGGCACTCGCAAGAGAAGGGGAGTATGGCGCtggggg                           >  1:954188/1‑150 (MQ=255)                                                                                                                         cGGGCGTTGCCGAAGTGGCGGCGGTTGGGGTGGAAAAAGGGCCGGAAAGGGAGGGGGCGGGGGCGTTTGTTCTTTCGAAAAAAAGAGGCAGTTTGGAAAATCGTGATGTGGCGCACTCGCCAGAGAAGGCGGTTATGGCGGtggtgg                           >  1:1183552/1‑147 (MQ=255)                                                                                                                           ggCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGGAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGAAAGAGAGGGGCAGTCTGGAAGATCGGGATGTGGGGCACTCGCAAGAGAAGGCGGTTATGGCGCtggggggc                         >  1:911979/1‑145 (MQ=255)                                                                                                                                 gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                <  1:52350/150‑1 (MQ=255)                                                                                                                                 gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTg               <  1:1418767/151‑1 (MQ=255)                                                                                                                                  ccGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTgg              <  2:232288/151‑1 (MQ=255)                                                                                                                                  ccGAAGTGGCGGTGGGTGGGGTGAAAGATGGGCTGAAAGGGCAGGTGGCGGTGGGGTTTGTCAATTCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGGGCACTCGCAAGAGAAAGAGATTTTGGGGCTGGGGGACAGCCCGAGTgg              >  1:670036/1‑151 (MQ=255)                                                                                                                                   cGAAGTGGCGGTGGTTGGGGTGAAAAATGCGCTGAAAAGGCAGGGGGCGGTGGCGGTTGTCATTTCGGAAGAGAGCGACAGTCTGGGAGATTGTGATGGGGGGCGCTCGCAAGAGAAGGCGGTTATGGCGCTGGTGGGCAGCCAGATTgg              >  1:420640/1‑150 (MQ=255)                                                                                                                                      aGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAAc          <  2:187845/151‑1 (MQ=255)                                                                                                                                               ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg  <  2:1386582/150‑1 (MQ=255)                                                                                                                                               ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg  <  1:369560/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCG  >  NC_000913/354138‑354429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑