Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	47.5%	108.6 / 27.6	80	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (7/35);  new base G (38/0);  total (45/35)
Fisher's exact test for biased strand distribution p-value = 5.20e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCG  >  NC_000913/354142‑354436                                                                                                                                                   |                                                                                                                                                     gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaaa                                                                                                                                                  <  1:980665/151‑1 (MQ=255)      tCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATg                                                                                                                                              >  2:1105510/1‑150 (MQ=255)       ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcg                                                                                                                                            <  2:847222/151‑1 (MQ=255)       ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATgcg                                                                                                                                            >  2:672214/1‑151 (MQ=255)          tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATgcg                                                                                                                                            >  2:706953/1‑148 (MQ=255)           gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGGCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAgcgc                                                                                                                                           >  1:751794/1‑148 (MQ=255)           gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTg                                                                                                                                         <  1:201104/150‑1 (MQ=255)           gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGa                                                                                                                                        <  1:965888/151‑1 (MQ=255)           gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGGAAGAGgcgc                                                                                                                                           >  1:762619/1‑148 (MQ=255)            aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                       <  2:986037/151‑1 (MQ=255)            aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                       <  2:1354686/151‑1 (MQ=255)                 gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGTGGTGGTTGGGGTGAAAGATGCGCTGAAAgg                                                                                                                                    <  1:890890/149‑1 (MQ=255)                  aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAggg                                                                                                                                   >  2:409898/1‑149 (MQ=255)                    ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCCGGAAggggcgg                                                                                                                               >  1:563125/1‑147 (MQ=255)                     gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggt                                                                                                                              <  1:639885/151‑1 (MQ=255)                     gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGAAAGGGGAgg                                                                                                                               >  1:862911/1‑150 (MQ=255)                       tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGAAAGGGGAggggg                                                                                                                            >  2:882364/1‑151 (MQ=255)                               tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAgggggggggggggggg                                                                                                                      >  2:948511/1‑136 (MQ=255)                               tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGGAAGGGGAgggggcggggggg                                                                                                                    >  1:1351352/1‑149 (MQ=255)                                aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGt                                                                                                                   <  1:1393168/151‑1 (MQ=255)                                   ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAAATGCGCGGGAACGGGAgggggcggggggggtt                                                                                                                 >  1:685354/1‑145 (MQ=255)                                     cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGttt                                                                                                                 <  1:338837/148‑1 (MQ=255)                                     cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTg                                                                                                                <  1:821118/149‑1 (MQ=255)                                     cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGGAAGGGGAGGTGGCGGGGGCGgtt                                                                                                                 >  2:780918/1‑148 (MQ=255)                                        gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCAtt                                                                                                           <  2:705419/151‑1 (MQ=255)                                           cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAATGCGCTGGAAAGGGAGGGGGGGGGGGGGGTTGTTAtttc                                                                                                         >  2:181903/1‑148 (MQ=255)                                           cACTGACGATGTAATTAACGGTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGGTGGAAGGGGAGGGGGGGGGGGGGGTTggtctttc                                                                                                         >  1:635441/1‑143 (MQ=255)                                            aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGGGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTCCtttc                                                                                                         >  1:709591/1‑147 (MQ=255)                                                  gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagaga                                                                                                 <  2:172572/151‑1 (MQ=255)                                                   aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagagag                                                                                                <  1:274004/151‑1 (MQ=255)                                                   aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTGtggtttcggaa                                                                                                     >  1:664657/1‑136 (MQ=255)                                                     gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGCGGTTGTCATTCCGGAaga                                                                                                   >  2:552048/1‑146 (MQ=255)                                                       aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGa                                                                                             <  2:564485/150‑1 (MQ=255)                                                       aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAGGCGCTGGAAGGGGAGGTGGCGGGGGGGGTTGTCATTCCGGAagaga                                                                                                 >  1:864828/1‑146 (MQ=255)                                                         ttAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                           <  2:943591/150‑1 (MQ=255)                                                                tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCCGGAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGGAAGAGAGCGGAAGTCTgg                                                                                     >  1:96407/1‑149 (MQ=255)                                                                 gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                                   <  2:1053999/150‑1 (MQ=255)                                                                  ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGGTTTTCCTTTCGAAAGAGAGCGACAGTc                                                                                        >  1:250465/1‑144 (MQ=255)                                                                   cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                                <  2:60559/151‑1 (MQ=255)                                                                   cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGCGCCGAAAAGGCAGGTGGGGGGGGGGGTTGTCATTTCCAAAAAGAGCGGAAGTCTGGaa                                                                                   >  1:1263434/1‑148 (MQ=255)                                                                     ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCg                                                                              <  2:655511/151‑1 (MQ=255)                                                                      gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGt                                                                             <  1:1197649/151‑1 (MQ=255)                                                                            gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                       <  1:1418309/151‑1 (MQ=255)                                                                            gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCggg                                                                            >  2:90905/1‑144 (MQ=255)                                                                               ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgc                                                                     <  2:475946/150‑1 (MQ=255)                                                                               ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGAAAGGGGAGGGGGCGGGGGCGGTTTTTATTTCGAAAGAGAGCGGCAGTCTGGGAGATCGTGGTGTgg                                                                      >  1:1417897/1‑149 (MQ=255)                                                                                 ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGCGGGGGGGGGGGGGGTTGTTATTTCGAAAGAGAGGGACAGTCTGGAAGATCGGGATGTGgcg                                                                    >  1:956664/1‑149 (MQ=255)                                                                                    cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACta                                                               <  1:1403839/151‑2 (MQ=255)                                                                                    cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                               <  1:122785/151‑1 (MQ=255)                                                                                    cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                               <  2:267118/151‑1 (MQ=255)                                                                                      cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGGGCCGAAAAGGGAGGTGGGGGTGGCGGTTGTCATTTCGGAAGAGAGCGACCGTCTGGAAGATAGTGGTGTGGGGCACCCGc                                                             >  1:558330/1‑151 (MQ=255)                                                                                          agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTTTCCTTCCGAAAGAGAGCGACAGTCTGGGAGAACGTGATGTGGGGCACTCGc                                                             >  1:89248/1‑147 (MQ=255)                                                                                           gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAAAGCGCCGTAAAGGGAGGTGGCGGTGGCGTTTGTCATTTCGAAAAAGGGCGCCAGTCTGGAAGATCGCGGAGTGGCGCCCTCGcca                                                           >  1:214820/1‑146 (MQ=255)                                                                                              tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAAAAGCGCCGAAAGGGGAGGGGGCGGGGGGGGTTGGCATTCCGAAAGAGAGAGACAGTCTGGGAGATAGGGGTGGGGCGCACTCGCAagag                                                        >  1:543218/1‑148 (MQ=255)                                                                                                aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                   <  2:1230356/151‑1 (MQ=255)                                                                                                 agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                  <  1:253288/151‑1 (MQ=255)                                                                                                      gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                             <  1:455950/151‑1 (MQ=255)                                                                                                       tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAAGGGGGGGGGCGGGGGGGGTTGTCATTCCCCAAGAGAGCGACAGTCTGGGAGGGCGTGATGTGGGGCCCTCTCCAGAGAAAGGGAatat                                             >  2:1418539/1‑150 (MQ=255)                                                                                                       tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGGAGGGGGCGGGGGCGGTTGTTATTCCGAAAGAGAGCGACAGGCTGGAAGATCGGGATGTGGGGCACTCGCCAGAGaaaggggtt                                               >  1:917206/1‑141 (MQ=255)                                                                                                         tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGAAGATGCGCTGAAAGGGCAGGTGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                               >  2:490341/1‑146 (MQ=255)                                                                                                         tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGTTGGGGGGAAAAATGCGCCCGAACGGGAGGGGGGGGGGGGGGTTGTCATTCCGCAAGAGAGCGACAGTCTGGAAGATAGGGGTGTGGCGGACCAGCCAGAGAAAGCg                                                  >  1:1148926/1‑143 (MQ=255)                                                                                                            ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgc                                          <  1:423685/148‑1 (MQ=255)                                                                                                            ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGGTTTTGgcg                                         >  2:913854/1‑149 (MQ=255)                                                                                                              aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAAAACGCCGGAAGGGGCGGGGGGGGGGGGGTTTGTCATTGCGGAAGAGAGGGGCAGTCTGGAAAAACGGGATGGGGGGCCCTCGCCAGAGAAAGCGATTATGGGGCtgg                                     >  1:636751/1‑151 (MQ=255)                                                                                                                ccATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtg                                   >  2:513781/1‑151 (MQ=255)                                                                                                                  aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCCGAAAGGGGAGGGGGGGGGGGCGTTTGGTATTCCGAAAGAGAGCGCCAGACTGGGAGATTGTGGTGTGGCGCACTCGCAAGAGAAGGCGGGTATGGCGCtggtg                                   >  1:258181/1‑149 (MQ=255)                                                                                                                  aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCCGGAAGGGGCGGGGGCGGGGGGGTTTGTCATTTCGAAAAAGAGCGACAGGCTGGAAGAACGTGGTGTGGGGCACTCGCAAGAGAAGGGGAGTATGGCGCtggggg                                  >  1:954188/1‑150 (MQ=255)                                                                                                                     cGGGCGTTGCCGAAGTGGCGGCGGTTGGGGTGGAAAAAGGGCCGGAAAGGGAGGGGGCGGGGGCGTTTGTTCTTTCGAAAAAAAGAGGCAGTTTGGAAAATCGTGATGTGGCGCACTCGCCAGAGAAGGCGGTTATGGCGGtggtgg                                  >  1:1183552/1‑147 (MQ=255)                                                                                                                       ggCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGGAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGAAAGAGAGGGGCAGTCTGGAAGATCGGGATGTGGGGCACTCGCAAGAGAAGGCGGTTATGGCGCtggggggc                                >  1:911979/1‑145 (MQ=255)                                                                                                                             gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                       <  1:52350/150‑1 (MQ=255)                                                                                                                             gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTg                      <  1:1418767/151‑1 (MQ=255)                                                                                                                              ccGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTgg                     <  2:232288/151‑1 (MQ=255)                                                                                                                              ccGAAGTGGCGGTGGGTGGGGTGAAAGATGGGCTGAAAGGGCAGGTGGCGGTGGGGTTTGTCAATTCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGGGCACTCGCAAGAGAAAGAGATTTTGGGGCTGGGGGACAGCCCGAGTgg                     >  1:670036/1‑151 (MQ=255)                                                                                                                               cGAAGTGGCGGTGGTTGGGGTGAAAAATGCGCTGAAAAGGCAGGGGGCGGTGGCGGTTGTCATTTCGGAAGAGAGCGACAGTCTGGGAGATTGTGATGGGGGGCGCTCGCAAGAGAAGGCGGTTATGGCGCTGGTGGGCAGCCAGATTgg                     >  1:420640/1‑150 (MQ=255)                                                                                                                                  aGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAAc                 <  2:187845/151‑1 (MQ=255)                                                                                                                                           ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg         <  2:1386582/150‑1 (MQ=255)                                                                                                                                           ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg         <  1:369560/150‑1 (MQ=255)                                                                                                                                               ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    >  1:296349/1‑151 (MQ=255)                                                                                                                                                 ggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcg  >  2:1418868/1‑151 (MQ=255)                                                                                                                                                 ggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcg  >  1:1169304/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCG  >  NC_000913/354142‑354436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑