Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,195	G→A	A298V (GCA→GTA)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,195	0	G	A	100.0%	283.5 / NA	75	A298V (GCA→GTA)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/34);  total (41/34)

CTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTA  >  NC_000913/3400047‑3400341                                                                                                                                                     |                                                                                                                                                   ctctTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTacg                                                                                                                                                  >  1:863125/1‑151 (MQ=255)           aaCAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCgg                                                                                                                                        >  1:839401/1‑151 (MQ=255)           aaCAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCgg                                                                                                                                        >  2:54674/1‑151 (MQ=255)             cAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTg                                                                                                                                      <  1:250106/151‑1 (MQ=255)             cAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTg                                                                                                                                      >  2:711297/1‑151 (MQ=255)                gTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGc                                                                                                                                   <  1:233645/151‑1 (MQ=255)                gTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGc                                                                                                                                   >  2:726927/1‑151 (MQ=255)                     cgccgTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCAt                                                                                                                              >  1:691829/1‑151 (MQ=255)                                tCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCgg                                                                                                                   >  1:944597/1‑151 (MQ=255)                                  gATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCgg                                                                                                                   >  1:78974/1‑149 (MQ=255)                                   aTCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGgaga                                                                                                                <  1:545606/151‑1 (MQ=255)                                      aTTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGt                                                                                                             <  2:813233/151‑1 (MQ=255)                                      aTTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGt                                                                                                             <  2:938669/151‑1 (MQ=255)                                       tttCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGATTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTc                                                                                                            >  2:191298/1‑151 (MQ=255)                                         tCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCg                                                                                                           <  2:1108465/150‑1 (MQ=255)                                         tCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCg                                                                                                           <  2:799765/150‑1 (MQ=255)                                          ccAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGa                                                                                                         <  2:390591/151‑1 (MQ=255)                                             gcgcTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGc                                                                                                      >  1:714218/1‑151 (MQ=255)                                              cgcTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGcc                                                                                                     >  2:795964/1‑151 (MQ=255)                                               gcTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGcc                                                                                                     >  2:483608/1‑150 (MQ=255)                                               gcTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGcc                                                                                                     >  2:1263124/1‑150 (MQ=255)                                                 tttGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGt                                                                                                  >  1:1160253/1‑151 (MQ=255)                                                    gccgccACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTcc                                                                                               >  2:754919/1‑151 (MQ=255)                                                     ccgccACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcg                                                                                              >  1:673986/1‑151 (MQ=255)                                                       gccACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcggc                                                                                            <  1:1397479/151‑1 (MQ=255)                                                       gccACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcggc                                                                                            <  2:1278356/151‑1 (MQ=255)                                                         cACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcggcgg                                                                                          >  1:234101/1‑151 (MQ=255)                                                          aCCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCggg                                                                                         >  2:898901/1‑151 (MQ=255)                                                          aCCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCggg                                                                                         >  1:1154412/1‑151 (MQ=255)                                                           ccGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCggg                                                                                         <  2:1403370/150‑1 (MQ=255)                                                              cgcgcCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACt                                                                                     <  2:722294/151‑1 (MQ=255)                                                                   cacacaGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTcc                                                                                >  1:201561/1‑151 (MQ=255)                                                                   cacacaGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTcc                                                                                >  1:1333396/1‑151 (MQ=255)                                                                     cacaGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTcc                                                                                >  1:1168997/1‑149 (MQ=255)                                                                     cacaGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAg                                                                              <  2:1430202/151‑1 (MQ=255)                                                                      acaGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGt                                                                             <  1:1280219/151‑1 (MQ=255)                                                                       caGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGt                                                                             <  2:1396586/150‑1 (MQ=255)                                                                             aGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAAcc                                                                       >  2:17918/1‑150 (MQ=255)                                                                              gCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCAt                                                                     >  1:440801/1‑151 (MQ=255)                                                                              gCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCAt                                                                     <  1:21896/151‑1 (MQ=255)                                                                                gggTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCAtt                                                                    >  2:998689/1‑150 (MQ=255)                                                                                 ggTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAc                                                                  >  2:964981/1‑151 (MQ=255)                                                                                  gTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAcc                                                                 >  2:468421/1‑151 (MQ=255)                                                                                  gTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAcc                                                                 >  1:1442125/1‑151 (MQ=255)                                                                                     ttCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACcgcg                                                              >  2:1447462/1‑151 (MQ=255)                                                                                       cAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCCACCATTACCgcgc                                                             >  2:140676/1‑150 (MQ=255)                                                                                       cAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCt                                                            >  2:550637/1‑151 (MQ=255)                                                                                        aGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTc                                                           <  2:462737/151‑1 (MQ=255)                                                                                            acaacGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTcaca                                                        >  1:1122805/1‑150 (MQ=255)                                                                                              aacGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATa                                                     <  1:210329/151‑1 (MQ=255)                                                                                                cGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATAcc                                                   <  2:102618/151‑1 (MQ=255)                                                                                                 gACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCg                                                  >  2:314830/1‑151 (MQ=255)                                                                                                 gACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCg                                                  <  2:1130175/151‑1 (MQ=255)                                                                                                  aCTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCgg                                                 <  1:31065/151‑1 (MQ=255)                                                                                                       aaTGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGc                                            <  2:913927/151‑1 (MQ=255)                                                                                                          gCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGt                                         <  2:1015087/151‑1 (MQ=255)                                                                                                           ccGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGtt                                        >  2:1009030/1‑151 (MQ=255)                                                                                                               tttCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTg                                    >  1:1186526/1‑151 (MQ=255)                                                                                                                ttcttcCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTg                                    <  2:180346/150‑1 (MQ=255)                                                                                                                  cttcCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCt                                     >  2:358264/1‑147 (MQ=255)                                                                                                                   ttcCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGt                                >  1:121351/1‑151 (MQ=255)                                                                                                                    tcCATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAg                                 <  1:696848/149‑1 (MQ=255)                                                                                                                     ccATTAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTg                               >  1:991998/1‑150 (MQ=255)                                                                                                                         tAACAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTc                           <  2:1126835/150‑1 (MQ=255)                                                                                                                          aaCAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGCGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCg                          <  2:944621/150‑1 (MQ=255)                                                                                                                           aCAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCg                          >  1:894195/1‑149 (MQ=255)                                                                                                                           aCAAACGGTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAg                        <  2:202444/151‑1 (MQ=255)                                                                                                                             aaaCGGTCAACGTTACGCACCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGACGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGCTCAGCGGTTCCTGCAGTGCTTCGAGGa                      <  1:429224/151‑1 (MQ=255)                                                                                                                                 ggTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTc                  <  1:805650/151‑1 (MQ=255)                                                                                                                                  gTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTc                  >  2:807009/1‑150 (MQ=255)                                                                                                                                  gTCAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCa                 <  1:446391/151‑1 (MQ=255)                                                                                                                                    cAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCAtt               <  1:1267010/151‑1 (MQ=255)                                                                                                                                    cAAGGTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGCCACTGTTCCAGTGCAACCCTTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCAtt               >  1:1006011/1‑151 (MQ=255)                                                                                                                                       ggTTACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGa            <  1:726910/151‑1 (MQ=255)                                                                                                                                          tACGCAGCAGTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGt          <  1:837261/150‑1 (MQ=255)                                                                                                                                                  agTACGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTa  >  1:495116/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTA  >  NC_000913/3400047‑3400341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑