Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	2,251,239	G→A	P28P (CCC→CCT)	LY180_10740 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	2,251,239	0	G	A	97.9%	98.9 / ‑4.9	47	P28P (CCC→CCT)	LY180_10740	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/22);  minor base T (1/0);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

CGGTCAGGCTCATGGGATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTGGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGATTTCGACCAGCTCCTT  >  CP006584/2250951‑2251471                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          cGGTCAGGCTCATGGGATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCCCGGTCAGCATCGGTGCCGTCCTGCGCGGCAGCACCTTAATCAGTGCATCGACCTCGGAGTTTGCGACCCCAGCCAGACTGACACCGCCCAGCGTCCCGGCCTTAGGTTtcctc                                                                                                                                                                                                                                  >  2:554471/1‑298 (MQ=255)          tCATGGGATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGAcc                                                                                                                                                                                                                         <  1:325883/298‑1 (MQ=21)            aTGGGATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTg                                                                                                                                                                                                                       >  1:473132/1‑298 (MQ=21)             tGGGATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGt                                                                                                                                                                                                                      <  1:478590/298‑1 (MQ=21)                    cggtttcggtGGCTGCCAGGGAAATATCACGGCCACC‑GCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATaa                                                                                                                                                                                                               <  2:280155/289‑1 (MQ=255)                         cTGATGGCCGCCAATGAAAAATCACTGCCCCA‑TCCGCCCATAGGTCATCCGACGAGAGAATTTTCGGGAACCGCACCGCACCCGGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCGGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGc                                                                                                                                                                                                          <  1:353910/298‑1 (MQ=11)                          tGATGGCGGCCAGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATGAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCt                                                                                                                                                                                                         >  1:166708/1‑298 (MQ=21)                            aTGGCGGCCAGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCCCTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTca                                                                                                                                                                                                       <  1:550587/298‑1 (MQ=21)                            aTGGCGGCCAGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTca                                                                                                                                                                                                       >  1:140714/1‑298 (MQ=21)                             tGGCGGCCAGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCTGCAGCACCTTAATCAGTGCATCGACTTCGGATTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTTGGTTTCCTCAGAGTGACCTGGTCGCTACCCCGCCcac                                                                                                                                                                                                      >  2:348909/1‑298 (MQ=11)                                  gcCAGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGc                                                                                                                                                                                                 <  1:101726/298‑1 (MQ=21)                                     aGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTtcat                                                                                                                                                                                                                                   <  2:602009/261‑1 (MQ=21)                                     aGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTtcat                                                                                                                                                                                                                                   >  1:602009/1‑261 (MQ=21)                                          aaTATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCCCCACAAGTTCAGGCAGTCCCAGTGCGGCGACCTCCTGCTCGGTCCGCCTCGGTACCGTCCTGCGCGGCAGCCCCTTAATCGGTGCATCGACTTCGGCGTTTCGCACCCCCGCCAAACTCACTCCGCGCACCGGCCCGGCCTTAGTTGTCCGCCCCGTCACCTGCTCCAACACCCGCTcaccaccttctacccg                                                                                                                                                                                         >  2:242168/1‑288 (MQ=11)                                            taccACTGCCACA‑CCCGCCTACAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACCAAAAACCGGCCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGAt                                                                                                                                                                                       <  2:262139/295‑1 (MQ=255)                                                aCTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTc                                                                                                                                                                                   >  1:77780/1‑298 (MQ=21)                                                 acgCCACAGTCCGCCCGCAGGTGCTCGACGGATGGCTGTGTCGGGCAGGGCCCTGCCCCGGGTGCGGCGACAAAAACCCGGCCACCCTACCGGCCAGCGCCACACGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCAGCGGTGCCGGCATGCGCGGCAGCACCTTAATCAGTGCATCGGCTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTc                                                                                                                                                                                   <  2:39980/296‑1 (MQ=11)                                                   gCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCGCCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTAACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAg                                                                                                                                                                                >  1:788516/1‑298 (MQ=21)                                                   gCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAg                                                                                                                                                                                <  1:237687/298‑1 (MQ=21)                                                                aTCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCCCAGCGGCCCGGCATCAGGTTTCATCCTCGTCACCTGTTCGATCACCTGCCTCCCACACCTTACCGCTTTGTCCAGGGTGATctcctt                                                                                                                                                                    >  2:617518/1‑293 (MQ=11)                                                                          cGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCCGTGCCGTCATGCGCGGCACCACCTTAATCAGTGCATCGACTTCGGCGTTTGCGACCGCAGCCAGACTGACACCGCCCAGCGTCCCGGCCTTAGGTTTCATCAGCGTCACCTGTTCGATAACCTGCTCACCACTCTTGCCCGAATTGTCCCGGGAAATCACATTTTCTTTGtta                                                                                                                                                          >  2:755096/1‑297 (MQ=11)                                                                             ccGGGAGGTGTTGCGGGACCGTCAGTAAACCGAGGTCGGGGGCGAAAACCCGACCGCCTTGCCGGCCGGCGGCACGAGGTCAGGCCGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCAGCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCAGCGACGTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGGCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATg                                                                                                                                                       <  2:143763/298‑1 (MQ=11)                                                                               gagagaTTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGt                                                                                                                                                     >  1:691076/1‑298 (MQ=21)                                                                                agagaTTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGtt                                                                                                                                                    <  1:8561/298‑1 (MQ=21)                                                                                 gagacgtgtCGGGAGCGTCGCGGCCACGAGGTGGGGGACAACAGGCCGGCCACGTTCCCGGCCGGCGCCACAGGGTCAGGGCGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCGTCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACGTCGGAGTTTGCGACCGCAGCCAGACGGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGttt                                                                                                                                                   <  2:84902/290‑1 (MQ=11)                                                                                  agaTTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGtttt                                                                                                                                                  >  1:397387/1‑298 (MQ=18)                                                                                   gaTTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTtc                                                                                                                                                 <  1:554471/298‑1 (MQ=18)                                                                                   gaTTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTtc                                                                                                                                                 >  1:158932/1‑298 (MQ=18)                                                                                                cGTCACCGACCCGAGTTCGGCGACAAAAACCCGCCCCCCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATc                                                                                                                                   <  2:77780/298‑1 (MQ=255)                                                                                                              gtccGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGCCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTCCATCCACCTCAGAGTTTTCGACCGCAACCCAACTGACACCGCCCAGCGCCCCCGCCTCAGGTTCACTCCGCCGTACCCGCTCCATAACCTGCTCACCCCGCCTGCACGGATACTGCCGGGTAAGCCCTTTTTCTTTTTTCATGATCTTCTCA‑TCCTCGCTTCGGGGTTAACCgcta                                                                                                                     >  2:669204/4‑295 (MQ=2)                                                                                                               ttCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCa                                                                                                                    <  1:702058/298‑1 (MQ=18)                                                                                                               ttCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGGTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCa                                                                                                                    >  1:144566/1‑298 (MQ=18)                                                                                                                     gACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGAGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGGTTTCTCACTTCT‑GCATCGGGGTTAACCGGTCAGCCAgg                                                                                                              >  1:206804/1‑298 (MQ=18)                                                                                                                          aaaaCCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGAc                                                                                                         >  1:420857/1‑298 (MQ=18)                                                                                                                                       ttACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGCCCCGGCATTAGGTTTCATCAGCGTGCCCGGTTCGCTAACCTCCTCACCACGCTTGACCGGATCGTCCAGCGTAATCACATTTCCTTTGTTCATGGTTCTCTCCCCTCT‑GAACCTGGGTTACCCGGTCAGCCACGCTGACCGGATGAAAATcc                                                                                            >  2:259675/1‑297 (MQ=11)                                                                                                                                                             cAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGgtgt                                                                      <  1:275238/298‑1 (MQ=18)                                                                                                                                                               ggCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTg                                                                    >  1:431845/1‑298 (MQ=18)                                                                                                                                                                gCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGc                                                                   <  1:242168/298‑1 (MQ=18)                                                                                                                                                                   gTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGTAAATCACAGGCCGATATTGCGGCGGTGTTGCTcc                                                                >  1:411616/1‑298 (MQ=18)                                                                                                                                                                     tCCAGGGCGGCGACCGCCTGCTCGGTCAGACTCGGGGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAg                                                              <  2:72035/298‑1 (MQ=12)                                                                                                                                                                         gTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGCCCGGATGAAAATCACAGGCCGATATTGCGGCCGTGTTGCTCCACCCgg                                                          >  2:813625/1‑298 (MQ=17)                                                                                                                                                                                         cTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACCGGACGCTATTGCGGCGGTGGTGGCCCAGCCGGTCAGCCCCGTTccact                                          >  2:274428/1‑294 (MQ=11)                                                                                                                                                                                          tCGGGCAGCATGGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGCCTTCGGAGGTTGCGACCGCAGCCAGACTGGCACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACGTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCtt                                         <  2:648256/298‑1 (MQ=9)                                                                                                                                                                                             gTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGCGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGCTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCCCAGGCCGATATTGCGGCGGTGTTGCCCCCGCCCGTCCACTCCGTTCACCTtctc                                      >  2:60901/1‑298 (MQ=255)                                                                                                                                                                                               cAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCaa                                    <  1:396948/298‑1 (MQ=17)                                                                                                                                                                                                                          caCCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGATTTCGACCa         <  1:348909/298‑1 (MQ=14)                                                                                                                                                                                                                                 aTCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGATTTCGACCAGCTCCtt  >  1:339247/1‑298 (MQ=14)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          CGGTCAGGCTCATGGGATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACA‑TCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTGGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCT‑GAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGATTTCGACCAGCTCCTT  >  CP006584/2250951‑2251471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑