Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	2,251,239	G→A	P28P (CCC→CCT)	LY180_10740 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	2,251,239	0	G	A	93.8%	58.3 / NA	33	P28P (CCC→CCT)	LY180_10740	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (10/20);  minor base C (1/1);  total (11/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGAGTTCGGTCAGGCTCATGGGATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACATCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTGGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGATTTCGACCAGCTCCTTACC  >  CP006584/2250944‑2251474                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            atgaGTTCGGTCAGGCTCATGGGATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACATCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGt                                                                                                                                                                                                                                          >  1:396916/1‑299 (MQ=21)                  cATGGGATACAGTTCTGATGGCGGCCAGTGAACTATCACTGCCACATCCCCCATCAGGTCATCGCCCGAGAGATTTTTCGGGCACGTCACTGCACCGAGTCCGGCGACAAACAACCCACCACCTTACCGCCCAGCCCCACACGGTCAGGCCGCCCCAGTGCGCCGCCCTCCCGCTCGCTCAGCATCCGTCCCCTCCTCCCCGCCCACCCATTACTCATCGCACCGCCTTCAGCTTGTGCGCCCCTCACCCGCCACACACCGCACAGCGTCCCGTCACTCGGTTtcccacgatgtaccta                                                                                                                                                                                                                         >  2:711893/1‑285 (MQ=2)                     gggATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACATCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTc                                                                                                                                                                                                                      <  1:44244/299‑1 (MQ=21)                      ggATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACATCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCg                                                                                                                                                                                                                     <  1:542335/299‑1 (MQ=21)                                cTGATGGCGGCCAGTGAAATATCACTGCCACATCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCt                                                                                                                                                                                                           >  1:684775/1‑299 (MQ=21)                                                            cacaTCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGt                                                                                                                                                                               <  1:605373/299‑1 (MQ=21)                                                                   gCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATcaca                                                                                                                                                                        >  1:86272/1‑299 (MQ=21)                                                                       tCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACAtttt                                                                                                                                                                    <  1:390616/299‑1 (MQ=21)                                                                          ggTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCCCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCCCCTTAATCAGTGCACCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGGTTCATCAGCGGGACCTGTTCGATAACCCGCTCACCACGCTTTGCCCGACTGTCCAGGGTAAATCCATTGTCtt                                                                                                                                                                 >  2:126719/1‑299 (MQ=11)                                                                                  accaaccagttgttCGGGAACATCAAGCCACCGAGTTCGGCGACAGAAACCCGCCCGCGCTACCGGCCAGCGCAACCAGGTCAGGCAGTGCCGGTCCGGCGACCTCCTGCGCGGTCAGCATCGGTGCCGGCATGCGCGGCAGCACCTGAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATg                                                                                                                                                         <  2:57342/287‑1 (MQ=11)                                                                                    cGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGt                                                                                                                                                       <  1:628403/299‑1 (MQ=21)                                                                                     gagagaTTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGtt                                                                                                                                                      <  1:461100/299‑1 (MQ=21)                                                                                      agagaTTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCCGCGCCACAAGGTCAGCCATTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCCGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACGTCGGAGTTTGCGACCGCAGCCCGACTGACACCGCGCAGCGCCCCGGCATTAGGTTTCACCCGCGTGACCTGGTCGAAAACCTTCTAGCCACGCTTGCCCGGATTTGCACGGGGAATTAACTTTTCTTTGTTAATGGttg                                                                                                                                                     >  2:285236/1‑298 (MQ=11)                                                                                       gagtgatttCGGGAACGTAAGGGAACCGGGTTCGGCTGCAAAAAAGCGACCTACTTACCGGCCAGCGCCACAAGGGCAGGGATGGCCGGTGCGTCGCCCTCCTGCTCGTTCAGCAGCGGTGCCGTCATGCGCGGCAGCACCTTAAGCAGTGCATCGACTTCGGGGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGtttt                                                                                                                                                    <  2:663277/293‑1 (MQ=11)                                                                                         gaTTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCACAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTtct                                                                                                                                                  <  1:110290/299‑1 (MQ=18)                                                                                                          aCTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGt                                                                                                                                 <  1:539086/299‑1 (MQ=18)                                                                                                                       cGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGcc                                                                                                                    <  1:504074/299‑1 (MQ=18)                                                                                                                                 aacccGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCg                                                                                                          <  1:153369/296‑1 (MQ=18)                                                                                                                                                   gCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCg                                                                                        <  1:650815/299‑1 (MQ=18)                                                                                                                                                     cAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGat                                                                                      >  1:38795/1‑299 (MQ=18)                                                                                                                                                           accgaGGTGAGGGACTTCCAGTCAGGCGTCCTCCTGCCTGGTCAGCATCGGAGCCGTGCTGCGGGGGAGCACCTTCATCAGCGCATCGACTGCGGAGTTTGCGAGCGCAGCCAGACTGACACCGCGCGGCGTGCTGGCATTAGGTGTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTTTGCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTATCCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTgcg                                                                                <  2:360730/295‑1 (MQ=11)                                                                                                                                                              aaGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTgcggcg                                                                             <  1:285236/299‑1 (MQ=18)                                                                                                                                                                          ttCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAg                                                                 <  1:711893/299‑1 (MQ=18)                                                                                                                                                                             cAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAAGCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTACCCGGACAGCCCGGCTGACCCGACGAAAATCACAGGCCGATATCGCGGCGgtggtgtcgccgccg                                                              >  2:165383/1‑290 (MQ=11)                                                                                                                                                                                       aCCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCg                                                    >  1:344467/1‑299 (MQ=17)                                                                                                                                                                                           cctcctCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCGTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGCCACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATACCCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCa                                                <  1:707986/299‑1 (MQ=12)                                                                                                                                                                                                     cagcggtggTGCGGTGGTGAGCGGCAGCACGTGAACCAGTGAATCGACTTAGGAGGTTGGGCCCGCAGCGAGACTGACACCGCGCAGCGTCGCGGCATTAGGGTTCATCAGCGTGACCTGTTCGATAACCTGATCTCCAGGCTGGACCGGAGTGTCCAGGGTAAGCACATTTTCTTTGTTCATGGGTTTCTCACTTCTGAATCGGGGTTAACCGGTCGGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCaag                                      <  2:591889/292‑2 (MQ=11)                                                                                                                                                                                                     cAGCATCGGTGCCGGCATGCCCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCTGCATTAGGTTTCATCAGCGTGACCCGTTCGTTAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTTTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAAt                                      <  1:707266/299‑1 (MQ=12)                                                                                                                                                                                                      aGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCCTTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGCCCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGCATCGGGGTTAACCCGTCACCCAGGCTGACCGGATGAAAATCACCGGCCTATATTGCGGCGCTGTTGCCCCAGCCGGTCCACGCCCTCCACCCTCTCaacc                                     >  2:541884/1‑297 (MQ=11)                                                                                                                                                                                                                     ggggtcgtggcaaCCTTGATCAGTGCATCGTGTGCGGAGTTTGCGACCGGGACCAGACTGGCACCGCGCTGCGTCCCGCTATTTGGTTTTATCAGCGGGCCCTATTTGATAACCTGCTAACCACGCTTGACCGGATTGTCCCGGGTAATCACATTTTCTTTGTTCAGGGTTTTCTCACTTCTGAGTCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCg                      <  2:652711/287‑1 (MQ=11)                                                                                                                                                                                                                             cagcaCCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGATTTCGAc              >  1:112193/1‑299 (MQ=14)                                                                                                                                                                                                                             atggaCCTTAATCCGTGTGTGGACTTCGGGGGCTGCGGCCGCAGCCGGACTGACGCCGGGCCGCGGCACGGCATTCGGTTGCATTAGCGGGAGCGGTTCATTAACCTGCTGTCCACGCTGGGCGGGATGGTCCAGGGTAATGACATTTTCTTTTTTCATGGTTTGCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGCCCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCGCAATCATGTTGATGGTGTCGATTTCGag              <  2:520974/295‑2 (MQ=11)                                                                                                                                                                                                                                         cAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGATTTCGACCAGCTCCTTAcc  <  1:23778/299‑1 (MQ=14)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            ATGAGTTCGGTCAGGCTCATGGGATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACATCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTGGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGATTTCGACCAGCTCCTTACC  >  CP006584/2250944‑2251474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑