Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	3,278,551	G→A	W246* (TGG→TGA)	LY180_15575 →	aldehyde oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	3,278,551	0	G	A	93.6%	145.6 / NA	48	W246* (TGG→TGA)	LY180_15575	aldehyde oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (26/18);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 8.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGAT‑CCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGGGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCT  >  CP006584/3278255‑3278841                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                    gCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGAT‑CCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTgagg                                                                                                                                                                                                                                                                                                   <  1:381269/299‑1 (MQ=255)                             caGGCGAAAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGAT‑CCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGAtt                                                                                                                                                                                                                                                                       <  1:89375/299‑1 (MQ=255)                               ggCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGAT‑CCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTgg                                                                                                                                                                                                                                                                     <  1:637599/299‑1 (MQ=255)                                    cAAGCCGTCGTCCCATTGTGCCCATCTTCAGCCCGTATTTGCCGTGCTCTTT‑CCGCGTCATCCTGTCCCCCTTCCTCCGCGTCAGGTGGCGAACGGCTGAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGAGGCCAAAATTCAGAACCGTTTCGCGGAAGGCATTTTGCGGACGCTGATCGAAGAGGGTCCGAAAGCCCTGAAAGAGCCAGAAAGCTACGATGTGCGCGCCAACGTGATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTg                                                                                                                                                                                                                                                                <  2:286953/299‑1 (MQ=255)                                          gtcggagcATTCGGACAATGTGCGGCGGGTATTGGCGGTGC‑GGATGCCGGTTGATCCATATACCCGGCCGCGGCGTCAGGTGGGTACCGGCGTAGAGGACGCCTTTTTACGCGCCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGGCCGGTTCGCAGAAGGAATTGTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTag                                                                                                                                                                                                                                                          <  2:169763/292‑2 (MQ=255)                                                          cATGTTCAGCCGGTATTTGCCGTGCTCGAT‑CCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGCGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAAc                                                                                                                                                                                                                                          <  1:23311/299‑1 (MQ=255)                                                                 cactgttgattGCCGCGGTCGTT‑CCGGTTGTTGCCCACCCCCTGCCGTCGGGTCAAGTGGCTAACTGCGTAGTGGAGTCCTTTGTACACACCGTGGAACAGTCTGTGACCAAACCGGTGGAGGACAACATTCAGGACCGTTTAGCAGAAGGCAGTTTGCTTACTGTGATCGGAGATTGTCCGAAAGCCCTGAAAGGGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATg                                                                                                                                                                                                                                   <  2:726996/290‑1 (MQ=255)                                                                    cGGTATTTGCCGTGCTCGAT‑CCGGTTTATACCTACACCCTGCCGCCGCGTCAGGAGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAACCTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTg                                                                                                                                                                                                                                <  1:413006/299‑1 (MQ=255)                                                                    cGGGATTTGGAGTGCCCCGT‑CCGGGTTATCCCTACACCCCGCCGCCGGGTCAGGTGGGGAACGGCGTAGGGGACGCCTTTGTACACACCGGGGAAAAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTGCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCGGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTg                                                                                                                                                                                                                                <  2:663107/299‑1 (MQ=255)                                                                     ggTATTTGCCGTGCTCGAT‑CCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTgg                                                                                                                                                                                                                               <  1:115566/299‑1 (MQ=255)                                                                                   tCGAT‑CCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTCTCGCAGAAGGCATTTTGCTGCCGCTGATCGAAGATGGTCCGAAAGCCCTCAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGCGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCACTGGCCTACCGCGCGACTGGGCAAAGCCTACGCCGGGCCACGAaccgact                                                                                                                                                                                                                 >  2:517343/1‑299 (MQ=255)                                                                                                  aCCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCt                                                                                                                                                                                                  <  1:109186/299‑1 (MQ=255)                                                                                                   ccTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTg                                                                                                                                                                                                 >  1:147027/1‑299 (MQ=255)                                                                                                        acCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGCGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCa                                                                                                                                                                                            <  1:275685/299‑1 (MQ=255)                                                                                                           cTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAACGAAGATGTTCCGAACGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACCCACGCACTGAACGGTTTGCTTGACGCTTGCGTACCCCAGAACCGGGCCACCCACATGCTGGCCCACAACATGACT‑GCAGTGCACGGGCTGGACCAcca                                                                                                                                                                                         >  2:682623/1‑297 (MQ=255)                                                                                                                             gCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGt                                                                                                                                                                       <  1:598007/299‑1 (MQ=255)                                                                                                                             gCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGt                                                                                                                                                                       >  1:294197/1‑299 (MQ=255)                                                                                                                                  cGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTcctgc                                                                                                                                                                  >  1:187333/1‑299 (MQ=255)                                                                                                                                   ggCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGATGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTGGATTGGCGCTGGCGTACCGCAGGACTGGGCAAGGCCAATTCCGGGCCAACAACGCACTGGGGAGCCACGG‑CGGGATCACGCCCAAACCCGGGccgacctcccgcc                                                                                                                                                                 >  2:32074/1‑287 (MQ=255)                                                                                                                                              cGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGa                                                                                                                                                      >  1:563791/1‑299 (MQ=255)                                                                                                                                                    tGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGaaa                                                                                                                                                <  1:178874/299‑1 (MQ=255)                                                                                                                                                      tACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGaaaaa                                                                                                                                              >  1:548185/1‑299 (MQ=255)                                                                                                                                                                ggAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCa                                                                                                                                    >  1:548394/1‑299 (MQ=255)                                                                                                                                                                  aaCAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAg                                                                                                                                  >  1:565135/1‑299 (MQ=255)                                                                                                                                                                          gTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAgcccgccac                                                                                                                          >  1:343163/1‑296 (MQ=255)                                                                                                                                                                             aCCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAgct                                                                                                                       <  1:517343/299‑1 (MQ=255)                                                                                                                                                                              ccAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAgctg                                                                                                                      <  1:276511/299‑1 (MQ=255)                                                                                                                                                                               cAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAgctgc                                                                                                                     >  1:214348/1‑299 (MQ=255)                                                                                                                                                                                aaaCCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAgctgct                                                                                                                    >  1:210274/1‑299 (MQ=255)                                                                                                                                                                                aaaCCGGGTGAAGCCGAAAGGCAGTACCGCTTCGCAGAAGGCATTTTGCTGACGCTGGTCGAAGATGGGCCGAAAGCCCTGAAAGGGCCAGAAAACTGCGATGTGCGCGCCAACGTCATGTGGGGTGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAgctgct                                                                                                                    <  2:266887/299‑1 (MQ=255)                                                                                                                                                                                       gtggtgaggtaaTTCAGGACGGTGGCGCAGCAGGGATGGTGCGCAGGGTGATCGGGTGTGGTGCGAAAGCCGGGAAAGCGGCGGAAGAGGACGATGTGGGCGCGAAGGTCGTGTGAGGGGGGAGGCAGGCGCGGAACGGTTTGATGGGCGCGGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCGCGCGCAAACACGGGCTAGCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAatag                                                                                                             <  2:194618/289‑2 (MQ=255)                                                                                                                                                                                            gCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGa                                                                                                        <  1:139536/299‑1 (MQ=255)                                                                                                                                                                                               aaaaTTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAAcg                                                                                                     >  1:324619/1‑299 (MQ=255)                                                                                                                                                                                                           cGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACAt                                                                                         >  1:13082/1‑299 (MQ=255)                                                                                                                                                                                                                  cAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGaa                                                                                  >  1:720942/1‑299 (MQ=255)                                                                                                                                                                                                                    gAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCCCTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAgg                                                                                >  1:154959/1‑299 (MQ=255)                                                                                                                                                                                                                     aaGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGt                                                                               >  1:33949/1‑299 (MQ=255)                                                                                                                                                                                                                            gcggcggACGCTCGTCGAGGATGTTCCGGAAACCCCGAAAGGGCCGGAAGTCACCTAGGTGCGCGCAGACGTCGTGGGGGGGGCGACGAATGCGCTGAACGGGTTGGGTGGAGGTGGCGTACCGAAGTACGGGGCAACGCATATGCGGGGGCACGAACTGACT‑GCGAGGTACGGTCGGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCgatg                                                                        <  2:56003/293‑1 (MQ=255)                                                                                                                                                                                                                                 tGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAg                                                                   >  1:546203/1‑299 (MQ=255)                                                                                                                                                                                                                                                    tCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTg                                                >  1:392608/1‑299 (MQ=255)                                                                                                                                                                                                                                                      cGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAACGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGcc                                              <  1:467921/299‑1 (MQ=255)                                                                                                                                                                                                                                                             ccTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAAccc                                       >  1:292033/1‑299 (MQ=255)                                                                                                                                                                                                                                                              cTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCg                                      >  1:287180/1‑299 (MQ=255)                                                                                                                                                                                                                                                                       ccAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCCGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCtt                             >  1:182312/1‑299 (MQ=255)                                                                                                                                                                                                                                                                            aacatACGAGGGGCGCGCCAGAGTCAGGGGGGGGGGGAGGCAGGCCGGGAACGGGTGTGTTGGCGCTGGCGTGCCGGAGGAGTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGc                        <  2:147027/295‑1 (MQ=255)                                                                                                                                                                                                                                                                               cTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAAt                     >  1:106937/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                    aTGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGc                >  1:514166/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                aCGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGAGCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAAAATCACTGAAAGTTCCGATGATGAGCGTATTGAAGCCGCGATTGCCTCAACCCCCAATTTCTTTGAGCAATTGAGCGTGCCGAccccc    >  1:595047/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                                  gTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCAAct  >  1:11876/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                    GCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGAT‑CCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGGGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACT‑GCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCT  >  CP006584/3278255‑3278841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑