Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	3,505,943	C→T	G41D (GGT→GAT)	LY180_16680 ←	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	3,505,943	0	C	T	98.3%	179.5 / NA	59	G41D (GGT→GAT)	LY180_16680	malate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (28/29);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

GCTTTTTTCAGCACTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTAATCAGGAACCTAACTTACGTAATTAACAGTCG  >  CP006584/3505661‑3506238                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                        gCTTTTGTCAGCACTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGc                                                                                                                                                                                                                                                                                         <  1:338269/299‑1 (MQ=255) ccTTTTTTCAGCACTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGCTAGCGATAATACCAATGCACGCGTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCCGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCTCTAACCCTGCAGAGATAAGAACGACACCTGCCCCTGCCACCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTAACAGCAGTAGGGATATGGCCCAGTACGACCGCCACATCGGCAGGCCATGGAgt                                                                                                                                                                                                                                                                                         >  2:127534/2‑298 (MQ=255)    tttttCAGCACTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGat                                                                                                                                                                                                                                                                                      <  1:561421/299‑1 (MQ=255)          aGCACTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTTCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATa                                                                                                                                                                                                                                                                                >  1:704832/1‑299 (MQ=255)          aGCACTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATa                                                                                                                                                                                                                                                                                >  1:441217/1‑299 (MQ=255)          aGCACTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATa                                                                                                                                                                                                                                                                                <  1:401019/299‑1 (MQ=255)          aGCACTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAATCGCCTCTTCACCAGAAAAACCTTTGATTTTCACAGACGTAGGGATAGGGCTCAGAACGACAGCCACATCGGCAGTCCCTGGAGCGagtacaca                                                                                                                                                                                                                                                                                >  2:291383/1‑292 (MQ=255)             aCTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACag                                                                                                                                                                                                                                                                             >  1:403517/1‑299 (MQ=255)               ttCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACagag                                                                                                                                                                                                                                                                           >  1:377804/1‑299 (MQ=255)                        aaTCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTg                                                                                                                                                                                                                                                                  <  1:38214/299‑1 (MQ=255)                             cAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGATACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTATGGATCTGCCTCAGAACGACAGCCACACCGGGAGTCACTGGAGCGAGAGAATACAGAGAGAGTTCTAAAcat                                                                                                                                                                                                                                                             >  2:44382/1‑297 (MQ=255)                               aCTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGCGTTCTGAACCTGa                                                                                                                                                                                                                                                           >  1:325189/1‑299 (MQ=255)                                cTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGaa                                                                                                                                                                                                                                                          >  1:715535/1‑299 (MQ=255)                                      tgtTAACCGGGTTAGTGATAACACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCACCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGt                                                                                                                                                                                                                                                    >  1:515364/1‑299 (MQ=255)                                                        taataCCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACagtagt                                                                                                                                                                                                                                  >  1:692186/1‑299 (MQ=255)                                                           taCCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCa                                                                                                                                                                                                                               <  1:195769/299‑1 (MQ=255)                                                             ccAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAg                                                                                                                                                                                                                             <  1:297076/299‑1 (MQ=255)                                                                    aCGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTgg                                                                                                                                                                                                                      >  1:461883/1‑299 (MQ=255)                                                                     cGCTTTCGGGCAGGTTTTCGAAACTTGGTGGACCAGGTCTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGc                                                                                                                                                                                                                     <  1:550798/299‑1 (MQ=255)                                                                                aGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCca                                                                                                                                                                                                          >  1:574213/1‑299 (MQ=255)                                                                                                cTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCAAGGACTg                                                                                                                                                                                          >  1:218429/1‑299 (MQ=255)                                                                                                          tttttCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCAt                                                                                                                                                                                <  1:252959/299‑1 (MQ=255)                                                                                                          tttttCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCAt                                                                                                                                                                                <  1:525669/299‑1 (MQ=255)                                                                                                             ttCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGTGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCt                                                                                                                                                                             <  1:687908/299‑1 (MQ=255)                                                                                                                    tGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTcc                                                                                                                                                                      <  1:26463/299‑1 (MQ=255)                                                                                                                      ccGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCtt                                                                                                                                                                    <  1:613359/299‑1 (MQ=255)                                                                                                                                             tgtaCGATCCATACCCGGTTTACGCGCTGCACCGGAAGAGATAAAAACTACATCTGCTCCTTCCTGCGCTGGAGTCGCAGTTTCACCAGAAAAACCTTTGAATTTCACAGCAGTAGGGATATGGCTCAGATCGGCAGCGACATCGAGAGTCACTGGAGCGATATCATACAGAGAGAGTGCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGatat                                                                                                                                             <  2:515364/296‑1 (MQ=255)                                                                                                                                                 actcacaatcccGGTTTACGGCCTACACCGGCAGAGTGACGAACGACATCTGCTCCTTCCCGCGCCGGAGTCGCATGTTCAACAGAAAGACCTTTGATTTTCACAGCAGTAGGGATATGGCTGAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTg                                                                                                                                         <  2:373524/290‑1 (MQ=255)                                                                                                                                                  gATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGc                                                                                                                                        >  1:558674/1‑299 (MQ=255)                                                                                                                                                           ccGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGcc                                                                                                                               <  1:392572/299‑1 (MQ=255)                                                                                                                                                               tttACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGa                                                                                                                           >  1:585026/1‑299 (MQ=255)                                                                                                                                                                          cacCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCcac                                                                                                                >  1:669923/1‑299 (MQ=255)                                                                                                                                                                             cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATCTGGCTCAGATCGCCAGCCACATCGGGAGTCACTGGACCGATGTCATACAGGGAGAGCTCTGAACCTGAAGGCAGTTGGGCTTTTCACAGTAGTGAAATCGCCTGGCCCATACCGCCACCAGAGCCCAGTCCCTCGACTCTCATCCTAAACTCCCGATTACATTGCGAAAATAAGAACTGTTGCTCCGCATCCCCGCCCTTAAACCACaaa                                                                                                             >  2:121528/1‑299 (MQ=255)                                                                                                                                                                             cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACaaa                                                                                                             >  1:673966/1‑299 (MQ=255)                                                                                                                                                                                               cATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGAc                                                                                           >  1:119569/1‑299 (MQ=255)                                                                                                                                                                                                        cTTCCGGTGTCGGGGTTGCATATTCACGAGGAAAACCTTTGATTTTGACAGCAGAAGGGAGATGGCTCAGAGCGACAGCCACGTCGGGGGTCGCTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGTTTTTTAACGGTAGTGCGAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACAtctcg                                                                                  <  2:441217/299‑2 (MQ=255)                                                                                                                                                                                                        cTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCa                                                                                  <  2:574213/299‑1 (MQ=255)                                                                                                                                                                                                                gcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCCGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTATCATCCTAAACTCCTTATTATATTTATAAACTAAACTATGTTACTCCGCTGCCGCGAACTTAATTCACAAAATTGACTGTTACAATTAACACAGCTCacaactaac                                                                          >  2:437486/1‑291 (MQ=255)                                                                                                                                                                                                                gcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAAtgtg                                                                          >  1:92453/1‑299 (MQ=255)                                                                                                                                                                                                                  cgtgtgcGCCTCTTCAACCGAAAAACCTTTAGTTTTCACAGCAGGAGGGAAATGGGTGTGATCGGCAGCCAAAACGGGAGTGAATGGAGCAGTATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTCAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTa                                                                        <  2:325189/293‑1 (MQ=255)                                                                                                                                                                                                                   ggggtgccATCTTTACCAGAAAATCCTTTGATGTTAACAGCAGTAGGGATATGGCTCAGAGCGACAGCACAATCGGGAGTGACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAg                                                                       <  2:461883/292‑1 (MQ=255)                                                                                                                                                                                                                         gCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGCAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCTAGGCATGCGAATTTCCTCCTAAACTCCTTATTATATTGATAACCTAAGACATGTTCACCCGCTGCCGCGACCATCATCCACATAAATTCTATTTATAATTACCACATCTCAATAATGTGTAGacaccc                                                                 >  2:608345/1‑297 (MQ=255)                                                                                                                                                                                                                          cATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTTAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCCACCTTAATCAACAAAATTTCCGTTTAACATGACCACACCCCAAACATGTTTAATCaacca                                                                >  2:103570/1‑295 (MQ=255)                                                                                                                                                                                                                               tCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGttt                                                           <  1:291383/299‑1 (MQ=255)                                                                                                                                                                                                                               tCAACAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGttt                                                           <  1:37053/299‑1 (MQ=255)                                                                                                                                                                                                                                cACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTa                                                          >  1:523369/1‑299 (MQ=255)                                                                                                                                                                                                                                 aCCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAg                                                         <  1:35512/299‑1 (MQ=255)                                                                                                                                                                                                                                   cAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCg                                                       <  1:464918/299‑1 (MQ=255)                                                                                                                                                                                                                                    aGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGt                                                      <  1:582790/299‑1 (MQ=255)                                                                                                                                                                                                                                            cTTTGATATACAAAGCAGGAGGGAGATGGCGCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATGATAGAGAGATAGTTCTGAGCCTAAAGGCAGTTGTGTTTTGAAGAGTAGTGCAAGCGCCTGGCCAAGACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAGATTGCCGGTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCAt                                              <  2:158670/299‑1 (MQ=255)                                                                                                                                                                                                                                                gATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTaa                                          <  1:69744/299‑1 (MQ=255)                                                                                                                                                                                                                                                    ttCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGACGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGTCCAAGCCCGCCAGCAGCGCCGAGGATTGCGACTTTCATCCTAAACTCCTTCTTATATTGATAAACTAAGATATGTTCCTCCGCTGCCCCGACCTTAATCCACCAAATTGCCATTTACAAATACCACAATCCAAGTATTATTAGACACCACACTTTCGCCTTAATGCATTTAAttaa                                      >  2:703771/1‑297 (MQ=255)                                                                                                                                                                                                                                                      cacaGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGCCTTTCATCCTAAAATCCTTCTTATCTTGATAAACTACGATATGTTGCTACTCTGCCGCGCCCTTAATCCCACACATTGCCGTTTACAATGACCACATCCCAAGAACGTTTAGTCACGCAAATTTAGCCGTTATCCATTTAAttacat                                    >  2:121296/1‑295 (MQ=255)                                                                                                                                                                                                                                                      cacaGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAACTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCg                                    <  1:127534/299‑1 (MQ=255)                                                                                                                                                                                                                                                        cagcagTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCCCATCCCCAGAATGTGTAGTCAACCAAGTTTAGCGTTTATGCATTTAATTTCCTTa                                  >  2:46905/1‑299 (MQ=255)                                                                                                                                                                                                                                                            agTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTAATCa                              <  1:44382/299‑1 (MQ=255)                                                                                                                                                                                                                                                                  gATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGCTTTTAACAGTAGTGCAAGCGCCTCGCCAATACCGCCAGCAGCGCCGAGGACTGCGCCTTTCATCCTAAACTCCCTATTATATTGATAAACTAAGATATGTTGCTCCTCTGCCCCGACCTGAATCCACAAACTTGCCGTTTCCAATGACCCCATCTCAAGAATGGGTAATCACGCAAGTTTAGAGTTTATCCATTTAATTCCCTGACTCAAGAAcc                        >  2:220222/1‑299 (MQ=255)                                                                                                                                                                                                                                                                               tCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTAATCAGGAACCTAACTTACGTAAt           <  1:121528/299‑1 (MQ=255)                                                                                                                                                                                                                                                                               gcGACAGTCAAATCGGGAGTCAGTTGAGCGATTTCAGACAGAGGGAGGTCTGAGACTTAAGGCATGTGGGTTTTTACCAGTAGTGCAATGTCCTTGCCAATAACGCCAGCAGCGCCGAAGATTGCGACTTTCATCCTAAACTCCTTAATATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTAATCAGGAACCTAACTTACGTAAt           <  2:334348/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                        acatCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTAATCAGGAACCTAACTTACGTAATTAACAGTCg  <  1:248846/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                        GCTTTTTTCAGCACTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTAATCAGGAACCTAACTTACGTAATTAACAGTCG  >  CP006584/3505661‑3506238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑