Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	4,469,025	+A	coding (748/1350 nt)	LY180_21345 →	permase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	4,469,024	1	.	A	90.0%	88.7 / 1.3	30	coding (747/1350 nt)	LY180_21345	permase
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base A (15/12);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑T‑A‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGC  >  CP006584/4468737‑4469300                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                      ctgctCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGgcgcg                                                                                                                                                                                                                                                                             >  1:297438/1‑298 (MQ=255)               tAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGt                                                                                                                                                                                                                                                               >  1:18179/1‑298 (MQ=255)                                    taaatccccGCTTTGGGGTCTGCCCGTTTGGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGGCACTGGTATGGCTGGCGGGGATCATCGGCCTTGAAAATCTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCTGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCt                                                                                                                                                                                                                                          <  2:297438/292‑1 (MQ=255)                                    tAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCt                                                                                                                                                                                                                                          <  1:326298/298‑1 (MQ=255)                                               ttGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACgg                                                                                                                                                                                                                               >  1:156279/1‑298 (MQ=255)                                               ttGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACgg                                                                                                                                                                                                                               >  1:215089/1‑298 (MQ=255)                                                                gcgcTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGGCGGTTTGCTCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCCTTGAGCGATGAAAACGGCAATTCACTGATTGGACGCCTTG‑ACAT‑TAA‑TGGGCCCGCTGAATCTTGTAGTCCTGCCAAGCGTTCTGGCTCGGGTGAGGGCAGCAGGTTTTGTAGCCAcc                                                                                                                                                                                                              >  2:159840/1‑298 (MQ=255)                                                                        tGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTAt                                                                                                                                                                                                      >  1:58146/1‑298 (MQ=255)                                                                                        ccGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGCCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCCTTTTCGCCATGCCTTCATTGAGCGATGAAAACGACAATTCACTGATTGGCAGCCTGG‑ACAT‑T‑ACTGGGCGCGCTGACTCCTGTAGTCCTGCCAAGCGTTCTGCCGCTGGTGATGCCGGCAGTTTTTGCTGCCACCGTAACTAGCCGTGCCGTCCCCGGc                                                                                                                                                                                      >  2:281384/1‑298 (MQ=255)                                                                                         cGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGcc                                                                                                                                                                                     <  1:159840/298‑1 (MQ=255)                                                                                           gttaTTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAg                                                                                                                                                                                   <  1:15885/295‑1 (MQ=255)                                                                                                       cTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑CCAT‑TAA‑TGGGCGCGCTGCATCCTGTAGTCCTGCCAAGCGGTCCGGCGCCGCTGATGACGGCAGTATTTGATGCCACCGGAACTTACCGTCCCGTCGCCCGCCAGGCTGACctccgt                                                                                                                                                                       >  2:196991/1‑294 (MQ=255)                                                                                                                       tGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCCGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGa                                                                                                                                                       >  1:331527/1‑298 (MQ=255)                                                                                                                                    ggAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTgg                                                                                                                                          <  1:271810/298‑1 (MQ=255)                                                                                                                                                        gtggCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACt                                                                                                                      >  1:126924/1‑298 (MQ=255)                                                                                                                                                                  gctgACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagcag                                                                                                            <  1:101040/298‑1 (MQ=255)                                                                                                                                                                   ctgACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGACTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAg                                                                                                                                                                                                                            >  2:31586/1‑185 (MQ=255)                                                                                                                                                                   ctgACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGACTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAg                                                                                                                                                                                                                            <  1:31586/185‑1 (MQ=255)                                                                                                                                                                      aCCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGtt                                                                                                        >  1:100440/1‑298 (MQ=255)                                                                                                                                                                             tCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTg                                                                                                 >  1:95084/1‑298 (MQ=255)                                                                                                                                                                                    ctcAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggt                                                                                          <  1:140526/298‑1 (MQ=255)                                                                                                                                                                                               gTTTGTACTTTGCTCCTAACGTCCAGTTCTCCGGCGTTTTCGCCATGCCTTAATTGAGGGATGAAAACGGCAATTCCCTGATTGGCAGCCTGG‑GCAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTc                                                                               <  2:126924/298‑1 (MQ=255)                                                                                                                                                                                                  tGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCgg                                                                            <  1:196991/298‑1 (MQ=255)                                                                                                                                                                                                                aactaccATTTTTCCGGCTTTTTGGCCGTGACTTACTTGAGCGAGGAAAACGGCAATTCACTGCTTGGCAGCCTGG‑ACCT‑TAA‑TGGGCCGGCGGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCGGGTGATGACGGCAGTATTTTATGTCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTGCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATc                                                              <  2:95084/293‑1 (MQ=255)                                                                                                                                                                                                                                   ttttCGCCATGCCTGCATTGGGCGAGCAAAACGGCGAGTCGCTTATTGGCAGCCGGG‑ACGTGA‑A‑CGTGCAGACTGAGTCCTGTAGTCCTGCCATGCGTTGTGGGGCTGGTGATGGCGGCAGTATGTGATGCCACCGGAACTATCCGGGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCTACGGTGGGAACGCACTGACCACTGACTCCATGCGCAGCGTTTTCTGTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGCGTCTGCGGCGGGTAcgg                                           <  2:156279/298‑1 (MQ=255)                                                                                                                                                                                                                                   ttttCGCCATGCCGTGATTAGGCAATGAAAACGGAAATTGCCTGATGGGCAGCCTGGCGCTG‑A‑A‑TGGGGGCGCTGAATCCTGTAGGCCGGCCAAGCGTTCTGGGGCTGGTGATGACGGCAGTAGTTGATGCCACCGGAACTATCCGTGCCGTCGCGGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcgg                                           <  2:215089/298‑1 (MQ=255)                                                                                                                                                                                                                                              ccTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGt                                >  1:74978/1‑298 (MQ=255)                                                                                                                                                                                                                                                ttCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGGaa                              >  1:123808/1‑298 (MQ=255)                                                                                                                                                                                                                                                                 aCGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTa             <  1:281384/298‑1 (MQ=255)                                                                                                                                                                                                                                                                            cTGATTGGCAGCCTGG‑ACAT‑TAA‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGc  >  1:117165/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                      CTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGG‑ACAT‑T‑A‑TGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCTGGTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGC  >  CP006584/4468737‑4469300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑