Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	10,637	C→A	G18G (GGC→GGA)	mogA →	molybdenum cofactor biosynthesis protein MogA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	10,637	0	C	A	100.0%	88.4 / NA	27	G18G (GGC→GGA)	mogA	molybdenum cofactor biosynthesis protein MogA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/15);  total (12/15)

AGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCA‑GTAGCGGACCGCGAGATGCCTGGCTT  >  CP006584/10348‑10879                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    aGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCa                                                                                                                                                                                                                                             <  1:168648/298‑1 (MQ=255)                ggCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATccc                                                                                                                                                                                                                              <  1:108064/298‑1 (MQ=255)                   agcaCAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTgc                                                                                                                                                                                                                           >  1:11406/1‑298 (MQ=255)                        aaCCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTgg                                                                                                                                                                                                                      <  1:181483/298‑1 (MQ=255)                                    aggcagggaGATAAACTGGGGAAAGGTTTACGTAAATGTGCTATTGACCAGGAAAAACGGGAAAAAATGATGGGCGATTTGCGGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGGAGCCTGTCTGTCACTCCCTTCGCATTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGa                                                                                                                                                                                                          <  2:331513/290‑1 (MQ=255)                                                aaaCTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTaa                                                                                                                                                                                              <  1:260348/298‑1 (MQ=255)                                                        ggAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTATGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATCAATACTTTACGTATTTGCTTAGTTTCCATCTCTGATCGCCCCTCCCGCGGAGTTTCGCAGGAAAAAGGCCTCCCTGGACTGGAGGAGTGGCTCACATCCGCCAGAACCACGCCg                                                                                                                                                                                      >  2:81009/1‑298 (MQ=255)                                                          aaGGTAGCCGTAAGTTGTCTATTTGCAAGGAATAACTGTCAAGAAAGATCGGCTGTCTGCTCTACGCATTCGTGGCGGTGA‑CGGGACGTTGGTGAGGGTACGCGTTTTGACGGTTGTCTGTCACTCCCTGTGCAGTTTATGATTCTTGTTCACGGTACTGTTTAAAAGGAAAAATCTTGATGAATACTTTACGTATTGGATTAGTGTCCATCTCTGATCGCGCATACAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGGCGtt                                                                                                                                                                                    <  2:221213/298‑1 (MQ=255)                                                           aGGTGTCCGTGAGTTGTGTATTGAACAGGAAAAACTGGAAAAAATGATCGGCGGTCTGGTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGttt                                                                                                                                                                                   <  2:111105/298‑1 (MQ=255)                                                                                      ggAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGAt                                                                                                                                                        <  1:261274/298‑1 (MQ=255)                                                                                            aCTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAg                                                                                                                                                  >  1:305227/1‑298 (MQ=255)                                                                                             cTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAgg                                                                                                                                                 >  1:296066/1‑298 (MQ=255)                                                                                                gAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGa                                                                                                                                              >  1:194852/1‑298 (MQ=255)                                                                                                 gaaaaaGGGTCGGGGTCCTGGTGTAATCATTCTTCGG‑GTGACCGGGAATTTGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCGGTTTAACGATACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGat                                                                                                                                             <  2:197394/297‑1 (MQ=255)                                                                                                    aaaTGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGCTCGCGCATCCAGCGGAGTTTATCCGGATAAAGGCATCCCAGCGCGCGAGGAATGGCTGACATCGGCGCTAACCACGCCGTATGAACTGTAACCCCGCTTAATCCACGATGAGCAggagctaac                                                                                                                                          >  2:297842/1‑291 (MQ=255)                                                                                                                 tCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACACCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTtg                                                                                                                             >  1:295631/1‑298 (MQ=255)                                                                                                                                    gC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACCGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGaaa                                                                                                          >  1:42105/1‑298 (MQ=255)                                                                                                                                                    cGGGCACGTTACCTCTTCTGTAGCCTGTCTGTCACTCCCTTCGCAGGGAATCATTCGGTTTAACGAGACTGTTTAAACGGTAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTgg                                                                                           <  1:66347/298‑1 (MQ=255)                                                                                                                                                                 tcttctGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACggg                                                                              >  1:228305/1‑298 (MQ=255)                                                                                                                                                                         aGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTg                                                                      <  1:48978/298‑1 (MQ=255)                                                                                                                                                                                                   gtATCATTCTGTTTAACGAGACTGTTTAAACGGTAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGccc                                            <  1:121632/298‑1 (MQ=255)                                                                                                                                                                                                   gtATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGccc                                            >  1:302072/1‑298 (MQ=255)                                                                                                                                                                                                    tATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTGACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGTGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCg                                           <  1:311800/298‑1 (MQ=255)                                                                                                                                                                                                             gtgtAAAGAGGCTGTTTAAATGGAAAAATTTTTATGAATAATTTACGTTTTGGGTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGc                                  <  2:296066/295‑1 (MQ=255)                                                                                                                                                                                                                        cTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCACATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCA‑GTAGCg                      <  1:270858/298‑1 (MQ=255)                                                                                                                                                                                                                                        aTCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCA‑GTAGCGGACCGCGAGATGCCTg      >  1:120571/1‑298 (MQ=255)                                                                                                                                                                                                                                            tgctgaATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATACAGCGGAGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACTCCGTTTGAACTGGACACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTCGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCCCGGGCGGAACTGGCCCGGCGCGTCTCGACGTAACCCCCGATGCGACGC‑GGCACGTCGCGGGCCGCGAGATGCCCGGCtt  >  2:37432/4‑298 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    AGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC‑GTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCA‑GTAGCGGACCGCGAGATGCCTGGCTT  >  CP006584/10348‑10879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑