Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,168,401	C→A	S606* (TCG→TAG)	LY180_05445 →	glucosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,168,401	0	C	A	95.7%	65.4 / NA	24	S606* (TCG→TAG)	LY180_05445	glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base A (14/8);  minor base G (0/1);  total (15/9)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTACATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCGGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGTTAGGGTTAGTGTGGGCTGTTGGGATGGCGTGGCTGGATCTGCGTTTCCTGTTCTGGCTGGCACCGATTGTCTTCTCGTTGATCCTGTCACCGTTTGTTTCGGTGATTTCCAGCCG  >  CP006584/1168112‑1168692                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                    ctgggttcGGTGTGCTTGGCGGGGGTGTTGTGTTATCTTGCCGCCCCGCTGTGGTTTATGTTCCTAGCGCTCTCTAATGAATTGGCGTGTGTACATTGGGTTACCTAACCCGAATACTTCCTGCAACCACGGCAGTTGTTCCCGGGTGGGCCGCTGTGGAGTCCTGAGCTGGCGATTGCACTTTTTGCTTCTACTATGGTGC‑TGTTGTTCCTGCCGAAGTGATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCGTCTGGCGCGTTACATTATAGTtgctgc                                                                                                                                                                                                                                                                                             <  2:289547/291‑1 (MQ=255)    cGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTACATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCGGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTgg                                                                                                                                                                                                                                                                                          >  1:49027/1‑298 (MQ=255)            gtTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTACATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCGGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCtt                                                                                                                                                                                                                                                                                  <  1:159467/298‑1 (MQ=255)              tCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTACATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCGGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCtttt                                                                                                                                                                                                                                                                                >  1:46880/1‑298 (MQ=255)                     gggCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTACATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCGGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGtg                                                                                                                                                                                                                                                                         >  1:301587/1‑298 (MQ=255)                             tGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTACATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCGGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGc                                                                                                                                                                                                                                                                 >  1:259535/1‑298 (MQ=255)                                       ctccgctccgCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTACATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCGGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGc                                                                                                                                                                                                                                                       >  1:293530/1‑298 (MQ=255)                                                          aTGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTACATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCGGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCt                                                                                                                                                                                                                                    <  1:57229/298‑1 (MQ=255)                                                               ccTCGCGCTCTCTACTGCATTGCAGGTAGTACATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCGGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCCGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGtt                                                                                                                                                                                                                               <  1:292452/298‑1 (MQ=255)                                                                   gcgcTCTCTACTGCATTGCAGGTAGTACATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCGGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTGCCATACGGTCTTCGTTGTCa                                                                                                                                                                                                                           >  1:54721/1‑298 (MQ=255)                                                                                                                                                             gtgcggctggAGCGGGCGATTCCACTTTTTGCTTCGACTAT‑GTGCGTTTTGTTCCTGCCGCAGTTATTGAGCATTTTGCTTAGTTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCt                                                                                                                                 <  2:293530/289‑1 (MQ=255)                                                                                                                                                                         cTGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGGGGTGTGGAATTAACAGCAGCGTGATGATGAGTCCCCTTCCTGGGGGGAAGCGTTCAAACGCCCCGGcgccaaggtgttgc                                                                                                                     >  2:242869/1‑285 (MQ=255)                                                                                                                                                                          tGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGtt                                                                                                                    >  1:299036/1‑298 (MQ=255)                                                                                                                                                                              gATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCGGTTAggg                                                                                                                >  1:292704/1‑298 (MQ=255)                                                                                                                                                                              gATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAAGTCACCGCAGCGTGATGATGACTCCACTTCCTGGTGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGTTAggg                                                                                                                >  1:299709/1‑298 (MQ=255)                                                                                                                                                                                      tttttGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGTTAGGGTTAGtgtg                                                                                                        <  1:242869/298‑1 (MQ=255)                                                                                                                                                                                                   tATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGTTAGGGTTAGTGTGGGCTGTTGGGATg                                                                                           >  1:315814/1‑298 (MQ=255)                                                                                                                                                                                                                    cTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGTTAGGGTTAGTGTGGGCTGTTGGGATGGTGTGGCTGGATCTGc                                                                           >  1:256740/1‑298 (MQ=255)                                                                                                                                                                                                                               ttggggattttttgttATCTGGGGAAAAGGAACGAAAGAATTGGGGGTGTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTGCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGTTAGGGTTAGTGTGGGCTGTTGGGATGGCGTGGCTGGATCTGCGTTTCCTGTTc                                                                <  2:299036/284‑1 (MQ=255)                                                                                                                                                                                                                                            ttttgtgggggCAAAGGAACGGAGGTATAGGGCGGCTTCTGGCTCGTGTCGTTATGGGTGGTGCTGGAAGTGTTTTGTTCCGTGCTGGTGGCTCCGGTACGCATGCTGTTGCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGTTAGGGTTAGTGTGGGCTGTTGGGATGGCGTGGCTGGATCTGCGTTTCCTGTTCTGGCTGGCACCGa                                                   <  2:181569/288‑1 (MQ=255)                                                                                                                                                                                                                                            cTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGTTAGGGTTAGTGTGGGCTGTTGGGATGGCGTGGCTGGATCTGCGTTTCCTGTTCTGGCTGGCACCGa                                                   >  1:181569/1‑298 (MQ=255)                                                                                                                                                                                                                                                                         cggcggCTTCTGGCGCGTAACATTATATTTGCTGCTGGAAGTGCTTTTTTCCTTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGTTAGGGTTAGTGTGGGCTGTTGGGATGGCGTGGCTGGATCTGCGTTTCCTGTTCTGGCTGGCACCGATTGTCTTCTCGTTGATCCTGTCACCgttt                      <  1:126392/298‑1 (MQ=255)                                                                                                                                                                                                                                                                              gcTTCTGGCGCGTTACATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCCTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGCGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTCCTGATAGGGTTAGTGGGGGCTGTTTGGCTGGCGTGGCCGCATCTGCGTCTCCTGTTCTGGCTGGCCCCGGTTTTCTTCTCGTTGTCCCTTCCACCgtttgtttt                 >  2:129470/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                             cATTATAGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGCAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGTTAGGGTTAGTGTGGGCTGTTGGGATGGCTGGGCTGGCTCTGCGTTTCCTGTTCTGGCTGGCACCGATTGTCTTCCGGTTGATCCTGTCACCTTTTGTTTCGGTTGTTTCCAGCCg  >  2:286796/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                    CACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTACATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCGGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACTATGGTGC‑TGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTTGTCAGCGCGTTCCTTGGCTGGGAAGTGGTGTGGAATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGTTAGGGTTAGTGTGGGCTGTTGGGATGGCGTGGCTGGATCTGCGTTTCCTGTTCTGGCTGGCACCGATTGTCTTCTCGTTGATCCTGTCACCGTTTGTTTCGGTGATTTCCAGCCG  >  CP006584/1168112‑1168692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑