Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,324,434	A→C	K833T (AAA→ACA)	narZ →	nitrate reductase A subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,324,434	0	A	C	100.0%	61.8 / NA	20	K833T (AAA→ACA)	narZ	nitrate reductase A subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/11);  total (9/11)

ATCCAGGGTAAAGATCTGGGGCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGC  >  CP006584/1324151‑1324689                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                aTCCAGGGTAAAGATCTGGGGCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGCATATGAACCCGTTAATTCACCCGCTGTCTGCGGCGGTCGATCTGGCCTGGGAAGCGAACAGCGACCGGGACATTGACAAACGCAACGCGAACGCATTCTCCGAAggtg                                                                                                                                                                                                                                                   >  2:273014/1‑295 (MQ=255)                      cAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAg                                                                                                                                                                                                                              >  1:119248/1‑298 (MQ=255)                       aaCAGGGTGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGCCTTACGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCACCCGTTTATTCACCCGCTGTCTGCGGCTGTCGAGCCGGCCTGGGAACAGACCAGCGACTGGGAAATGTACAAACCCATCGCGAGAACAATCAACCAAGTGGGCGTCGGTCAtacgggacaaga                                                                                                                                                                                                                             >  2:125520/1‑287 (MQ=255)                        aCAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTTCGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGaa                                                                                                                                                                                                                            >  1:147340/1‑298 (MQ=255)                               ggcgTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACa                                                                                                                                                                                                                     <  1:273014/298‑1 (MQ=255)                                          ggAAGAAGTGGTCTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTg                                                                                                                                                                                                          >  1:170463/1‑298 (MQ=255)                                              gaagTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa                                                                                                                                                                                                      <  1:42283/298‑1 (MQ=255)                                                                      cTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAAc                                                                                                                                                                              <  1:221294/298‑1 (MQ=255)                                                                                       tCTGGTGGTTACGCTGGACTTCCGTCTTGCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAt                                                                                                                                                             <  1:178532/298‑1 (MQ=255)                                                                                         tggtggTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGCAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCCAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACCCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGCtgt                                                                                                                                                           >  1:191672/1‑298 (MQ=255)                                                                                              gtTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGGGAAAg                                                                                                                                                      >  1:265198/1‑298 (MQ=255)                                                                                                               tCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGaa                                                                                                                                                                               >  2:108583/1‑256 (MQ=255)                                                                                                               tCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGaa                                                                                                                                                                               <  1:108583/256‑1 (MQ=255)                                                                                                                                                                                      aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAACGCGACTGGGAAATTTACACAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAACGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATGATGGTCGTCGAGCGCGATTATCAGGCGACTTACgc                                                              >  2:189010/1‑297 (MQ=255)                                                                                                                                                                                          ttCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGc                                                          <  1:125520/298‑1 (MQ=255)                                                                                                                                                                                                                                      aTCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCgg              <  1:82286/298‑1 (MQ=255)                                                                                                                                                                                                                                       tCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGt             <  1:189010/298‑1 (MQ=255)                                                                                                                                                                                                                                          ggCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTaaa          <  1:316976/298‑1 (MQ=255)                                                                                                                                                                                                                                                 gAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTg   <  1:325061/298‑1 (MQ=255)                                                                                                                                                                                                                                                  aaGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGc  <  1:246166/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                ATCCAGGGTAAAGATCTGGGGCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGC  >  CP006584/1324151‑1324689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑