Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,335,968	A→G	intergenic (+281/+21)	LY180_06295 → / ← LY180_06300	thymidine kinase/bifunctional acetaldehyde‑CoA/alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,335,968	0	A	G	100.0%	89.0 / NA	26	intergenic (+281/+21)	LY180_06295/LY180_06300	thymidine kinase/bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

CGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCGGGCTATATACCCTTCTCGGCAGTTTTTTAACGCCGCTATACGCCTCACAGGGCTCTTAAGCACCGACGTTGACTTGTGACCTGTAAAGTACAATATCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATATTGCCAGACAGCGCTACTGATTAAGCGGATTTTTTCGCTTTTTTCTCAGCTTTAGCCGGAGCGGCTTCTTTTTTCGCTGCAGTTTCACCTTCTACATAATCACGACCGTAGTAGGTATCCAGCAGGATCTGTTTCAGCTCGGAGATCAGCGGGTAACGCGGGTTAGCGCCGGTGCACTGGTCATCGAACGCATCTTCAGACAGTTTATCCACGTTCGCCAGGAAGTCTGCTTCCTGAACGCCAGCTTCACGGATAGATTTCGGAAT  >  CP006584/1335681‑1336234                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                           cGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCGGGCTATATACCCTTCTCGGCAGTTTTTTAACGCCGCTATACGCCTCACAGGGCTCTTAAGCACCGACGTTGACTTGTGACCTGTAAAGTACAATATCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACa                                                                                                                                                                                                                                                                  <  1:98500/298‑1 (MQ=255)    taaataaGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCATGACAGTTCGTTTTACGCACTTCTTCCGTGCTATCTACCCTTCTAGTCAGTTTTTTAACGCCGCTATACGCATCACAGGGCTCTTCAGCAACGAAGTTGACTTGTGCCCTGTAAAGTACAATATCCCTGAGTTTAGGCGTTATACATCGTCGCAACTACGATGAAGGCTAATGCTGTCGGTTTATGGAAAATGTGCTTTGAGTCAACAAAACAACTGCCCCAAAAGGATCTGTGTATGTTGTCAGagcgac                                                                                                                                                                                                                                                               >  2:258911/3‑293 (MQ=255)                 cTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCGGGCTATATACCCTTCTCGGCAGTTTTTTAACGCCGCTATACGCCTCACAGGGCTCTTAAGCACCGACGTTGACTTGTGACCTGTAAAGTACGATATCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaa                                                                                                                                                                                                                                                  <  1:46355‑M1/298‑7 (MQ=255)                                                 ccTGTCAGTTCGTTTTACGCACTTCTTCCGGGCTATATACCCTTCTCGGCAGTTTTTTAACGCCGCTATACGCCTCACAGGGCTCTTAAGCACCGACGTTGACTTGTGACCTGTAAAGTACAATATCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgtt                                                                                                                                                                                                                  <  1:247742‑M1/298‑39 (MQ=255)                                                           ctttttacGCACTTCTTCCGGGCTATATACCCTTCTCGGCAGTTTTTTAACGCCGCTATACGCCTCACAGGGCTCTTAAGCACCGACGTTGACTTGTGACCTGTAAAGTACAATATCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaactttttt                                                                                                                                                                                                        <  1:104615‑M1/296‑49 (MQ=255)                                                              tttACGCACTTCTTCCGGGCTATTATGCCTGCTCGGCAGTTTTTTCACGCCGCTATACGCCTCACAGGGCTCTTAAGCATCGACGTTGACTTGTGACCTGTAAAGTACAATATCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagt                                                                                                                                                                                                     <  2:146765‑M1/298‑52 (MQ=255)                                                                                  tatataCCCTTCTCGGCAGTTTTTTAACGCCGCTATACGCCTCACAGGGCTCTTAAGCACCGACGTTGACTTGTGACCTGTAAAGTACAATATCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacg                                                                                                                                                                                 >  1:252667‑M1/1‑227 (MQ=255)                                                                                       cccctTCTCGGCAGTTTTTTAACGCCGCTATACGCCTAACAGGGCTCTTAAGCACCGACGTTGACTTGTGACCTGTAAAGTACAATAGCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagt                                                                                                                                                                            <  1:174121‑M1/297‑77 (MQ=255)                                                                                                   aGTTTTTTAACGCCGCTATACGCCTCACAGGGCTCTTAAGCACCGACGTTGACTTGTGACCTGTAAAGTACAATATCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaaacatctgctcgaatacgagagtatagtcagtgcg                                                                                                                                                                >  1:255233‑M1/1‑210 (MQ=255)                                                                                                                   tataCGCCTCACAGGTCTCTTAAGCACCTACTTTGACTTGTGACCTGTAAAGTACAATATCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAATGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaa                                                                                                                                                <  1:167431‑M1/298‑105 (MQ=255)                                                                                                                       ctcctcACAGGGCTTTGAAGCACCGAGTTTGGTTTGTGACCTGTAAAATACAATATCCGTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTGTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaaccta                                                                                                                                            <  2:222994‑M1/296‑109 (MQ=255)                                                                                                                               aGGGCTCTTAAGCACCGACGTTGACTTGTGACCTGTAAAGTACAATATCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCAGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaataagagaggatagtcagtgcggtgatgatttgcttaacccaagaaaaac                                                                                                                                    >  2:257986‑M1/1‑182 (MQ=255)                                                                                                                                                  gTTGACTTGTGACCTGTAAAGTACAATATCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaacctatgaaaatcaaaagcttactcgcgctca                                                                                                                 >  1:131304‑M1/1‑163 (MQ=255)                                                                                                                                                                                     gtTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaacctatgaaaatcaaaagcttactcgcgctcacactcactgtgatttactaaaagagtttaaacatt                                                                              >  1:56041‑M1/1‑128 (MQ=255)                                                                                                                                                                                      tttAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaattccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaacctatgaaaatcaaaagcttactcgcgctcacactcactgtgatttactaaaagagtttaaacatta                                                                             <  1:247300‑M1/298‑172 (MQ=255)                                                                                                                                                                                             gTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaacctatgaaaatcaaaagcttactcgcgctcacactcactgtgatttactaaaagagtttaaacattagagttat                                                                      <  1:145359‑M1/298‑179 (MQ=255)                                                                                                                                                                                                     tcgtcgCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaacctatgaaaatcaaaagcttactcgcgctcacactcactgtgatttactaaaagagtttaaacattagagttattatctcta                                                              >  1:16955‑M1/1‑112 (MQ=255)                                                                                                                                                                                                               tgtgagtaaggttaatGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTATACAAAAAAACGGCCCCAGAAGGGGCCGTTGATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgatctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaacctatgaaaatcaaaagcttactcgcgctcacactcactgtgatttactaaaagagtttaaacattagagttattatctctaatgcgtcact                                                    <  2:215003‑M1/291‑197 (MQ=255)                                                                                                                                                                                                                       aGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgcatacgagagtatagtcagtgcggtgatgatttgcttaacctatgaaaatcaaaagcttactcgcgctcacactcactgtgatttactaaaaaagtttaaaaattaaagttattaactctaatgcttcccttccagggt                                            >  2:47353‑M1/1‑94 (MQ=255)                                                                                                                                                                                                                         gCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaataatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaacctatgaaaatcaaaagcttactcgcgctcacactcactgtgatttactaaaagagtttaaacattagagttattatctctaattcgtcactttccgttgcc                                          >  2:270820‑M1/1‑92 (MQ=255)                                                                                                                                                                                                                           tAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaacctatgaaaatcaaaagcttactcgcgctcacactcactgtgatttactaaaagagtttaaacattagagttattatctctaatgcgtcacttccaggtggcgt                                        <  1:43062‑M1/298‑209 (MQ=255)                                                                                                                                                                                                                           tAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaacctatgaaaatcaaaagcttactcgcgctcacactcactgtgatttactaaaagagtttaaacattagagttattatctctaatgcgtcacttccaggtggcgt                                        >  1:209425‑M1/1‑90 (MQ=255)                                                                                                                                                                                                                                            ggAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaacctatgaaaatcaaaagcttactcgcgctcacactcactgtgatttactaaaagagtttaaacattagagttattatctctaatgcgtcacttccaggtggcgtaagcaagattactcact                       <  1:257986‑M1/298‑226 (MQ=255)                                                                                                                                                                                                                                                          gggTAAACAAAAAAACGGCCACAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattactcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgactacgaaagtatagtcagtgcgctgatgatttgcttaacatatgaaaatcaaaagcttcatcgcgctcacactcacgttgatttactcaaagagtttacacattagagttattatctctatttcgtaacttacagggggcctaagcaagattacctcattctgggtactattt         >  2:309868‑M1/1‑59 (MQ=255)                                                                                                                                                                                                                                                              aaaCAAAAAAACGGCCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGAgcctaattaattaatcccaatgctctcctgataatgttaaacttttttagtaaatcatctgctcgaatacgagagtatagtcagtgcggtgatgatttgcttaacctatgaaaatcaaaagcttactcgcgctcacactcactgtgatttactaaaagagtttaaacattagagttattatctctaatgcgtcacttccaggtggcgtaagcaagattactcacttctgggtactgattacgt     >  1:40561‑M1/1‑55 (MQ=255)                                                                                                                                                                                                                                                                 cAAAAAAAAGGCAACATATGGGGACATGTATGTGTCCAGACAGCGGTACTGAacctaatgaattaatcccaatgctcttcggataattgtaatctattttagcaaatcatctgctcgaatacgagagtagagtcagtgcggtgatgatttgcttaacctatgaaaaacaaaagcttactcgcgctcacactcactgtgatttactaaaagagtttaaacattagagttattatctctaatgcgtcacttccaggtggcgtaagcaagattactcacttcttggtactgattacgtgat  <  2:131304‑M1/298‑247 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                           CGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCGGGCTATATACCCTTCTCGGCAGTTTTTTAACGCCGCTATACGCCTCACAGGGCTCTTAAGCACCGACGTTGACTTGTGACCTGTAAAGTACAATATCCCTGTGTTTAGGCGTTATACATCGTCGCAAATATGATGAAGGCTAATGCTGTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAAACGGCCCCAGAAGGGGCCGTTTATATTGCCAGACAGCGCTACTGATTAAGCGGATTTTTTCGCTTTTTTCTCAGCTTTAGCCGGAGCGGCTTCTTTTTTCGCTGCAGTTTCACCTTCTACATAATCACGACCGTAGTAGGTATCCAGCAGGATCTGTTTCAGCTCGGAGATCAGCGGGTAACGCGGGTTAGCGCCGGTGCACTGGTCATCGAACGCATCTTCAGACAGTTTATCCACGTTCGCCAGGAAGTCTGCTTCCTGAACGCCAGCTTCACGGATAGATTTCGGAAT  >  CP006584/1335681‑1336234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑