Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,459,895	C→T	T49T (ACC→ACT)	LY180_06935 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,459,895	0	C	T	97.0%	101.3 / NA	33	T49T (ACC→ACT)	LY180_06935	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (16/16);  minor base G (0/1);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGAAAACATTGCTCGGCGAGAGGATTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACCTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACCAGCAGAGCCGAAAGC  >  CP006584/1459607‑1460188                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                      aTGAAAACATTGCTCGGCGAGAGGATTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGtgtg                                                                                                                                                                                                                                                                                              >  1:30820/1‑298 (MQ=255)                  cGAGGGGATTAGGGGTGGCAGGACCGTGAACGGGGGGGGATTGGTGAATTTTAACTGGGGGGCGTGGGGGCCGAAGGTCATCCAGCAAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTGTTGACGCACTGAAAGCAATGGGAAGAGCCACAGCCATAGAACTTGCTGCGCGACTTGAGATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTca                                                                                                                                                                                                                                                                             <  2:30820/298‑1 (MQ=255)                         aTTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGaa                                                                                                                                                                                                                                                                      <  1:241920/298‑1 (MQ=255)                                 tCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAAcagccagc                                                                                                                                                                                                                                                              >  1:268704/1‑298 (MQ=255)                                     aTGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCg                                                                                                                                                                                                                                                          >  1:65678/1‑298 (MQ=255)                                            tGAACGGCGGGCGATGGGTAAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGTAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCTACTTGATATCAGCCGTGAATAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAAc                                                                                                                                                                                                                                                   <  1:139084/298‑1 (MQ=255)                                             gAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACt                                                                                                                                                                                                                                                  <  1:113346/298‑1 (MQ=255)                                                                        cggggAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGt                                                                                                                                                                                                                       <  1:21968/296‑1 (MQ=255)                                                                         tGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTc                                                                                                                                                                                                                      >  1:173443/1‑298 (MQ=255)                                                                           ggAGACCTTGCGTCCGAATTTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACAGGGAGAAAAATTTAATTGAGACTGTGTTTTACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTcgc                                                                                                                                                                                                                    <  2:268704/298‑1 (MQ=255)                                                                                             tGTCGACGAGCCAGGAAGTGCGATGTAATGTTTACCGGGGGAAAAATGTAAGGGGGAATGTTGGTGACTGAGGGAAAGCAATAGGAAAAGCCACATCCATAAAACTTGCTGCGCGCGTTGATATCAGCCGTGAAGAAGTGCTGAACGACCGATGGGAACTGAAAAGTGCTGGTGTCGTTGATAAAAGCGCGTACACTTGGCGTGAGGCGGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGa                                                                                                                                                                                                  <  2:115289/298‑1 (MQ=255)                                                                                                       ccagGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTACTGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAAATATGGGAACTGAAAAAGCCGGGTTTCGTTGATAAAAGCGAGTACACTTGGCGTTTGGCTGGTAAAAACGTTCCGAAGGACCCGACAGCGCCACCAGACATACCGGAAAAAACTACCAACGAAACAATAGCCCAAATTTCAAGGAGCGACTTAACAGcg                                                                                                                                                                                        >  2:308915/1‑298 (MQ=255)                                                                                                             aTTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGCAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGAtt                                                                                                                                                                                  >  1:201426/1‑298 (MQ=255)                                                                                                              ttGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAAGTATGGGAACTGAAACAGGCTGGTTTCGTTGATACACGCGCGGACACTTGGCGTGTCGCTGTTAAAAACGGTCAGCAAGAAAAACCCACGACCGCACAACCGCAGGAAGAAAATACCACGGAACAAGTCCCGCAAACTTCCAACAGCGATTGAACTGcgagaatag                                                                                                                                                                                 >  2:213393/1‑292 (MQ=255)                                                                                                              ttGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTg                                                                                                                                                                                 <  1:233321/298‑1 (MQ=255)                                                                                                                                 ggggtaaaagatgaaTGGGGACTGTTGGTGAGTGACGGAAAGCAAAGGAAAAAGCAGCATCCAACGAACTGCGGGCGCGACGTGGTATCAGCCGTGAGGAAGGGCTGAACGAACTATGGGATGGGGAAAAGGCTGGTTGGGTTGATAAAAGCGCGTACGATAGGCGTGTGGGTGATAACAACGGTCAGCAGGAGCAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTCCCGCGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAAc                                                                                                                                                              <  2:88087/285‑1 (MQ=255)                                                                                                                                          aTTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGa                                                                                                                                                     >  1:22918/1‑298 (MQ=255)                                                                                                                                                   agaCTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGCACTGAAACAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCATGTGGATGATAACAAAGTTCAACAGGAACAGCCAGCGCCGACAGAACTGCACGAAGACACTCCCACGGCACCAGTCGCAAAACTTTAAGCGGCCGAATTAACTGCCACCATTGAAAAACGTGCACCACTGACTGCggctaatatggagcg                                                                                                                                            >  2:150102/1‑285 (MQ=255)                                                                                                                                                                 gaaatGAAATCAATTGGAAAAGCCTCATACATAGAAATGTCTGCGCGACTTGATATATGTCGTGAAGTAGGGATGAACGAACTTAGGGAAATGAAAAAGTCTGGTTTCGTTGATAACAGCGCGTACTATTGGCGGGTGGCTGAGAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACGGCCGGAATAAACTACCACGGCAACAGTCGCGAAAATTTCATAGAGCGATTTAACTGCGATGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGACTACGTTGGTCGGTACca                                                                                                                              <  2:22918/294‑1 (MQ=255)                                                                                                                                                                              tGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGt                                                                                                                 >  1:105429/1‑298 (MQ=255)                                                                                                                                                                                  aaaaGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCt                                                                                                             <  1:119364/298‑1 (MQ=255)                                                                                                                                                                                                                             cGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTACGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTcgcg                                                                  >  1:152658/1‑298 (MQ=255)                                                                                                                                                                                                                               tGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCCGGACCAGCCCGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGCGAGCGATTTAACTTCGACGATTGACCAACGTGTTCCACAAACTGCGGATGAACTGGCTACGTTGTACGGTACCACAACCAGCACATTGGCTTCAACCCTGGCTATGGCATCCAACAACGCTcacacgatgcgcagt                                                                >  2:248995/1‑284 (MQ=255)                                                                                                                                                                                                                                   gaagTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATc                                                            <  1:213393/298‑1 (MQ=255)                                                                                                                                                                                                                                    gagTGCTGAACGAAGTTTGGGATATAAGAGAAGTTGTTGTCGTTGATAAAGGCGGGTAGACTTGGGGTGGGGCTGATAACAAGGTCGAGCGGGGACAGCCAGCGCCGGCATGAGTGCCGGAAGAAACTACGACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAAATGCGACGATTGAACAACGTGGTCCACGAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCGATCAGCAAAGCTCGTCTGATTCGCGTTAATCa                                                           <  2:173443/297‑1 (MQ=255)                                                                                                                                                                                                                                                        gAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACt                                       <  1:308915/298‑1 (MQ=255)                                                                                                                                                                                                                                                          aCTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACCCTTGGCGTGTGGCTGATAACAACGTTCAGCAGGCACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGAAACAGTCGCGAAAATTTCATCGAGCGATTTAACTGCGACGATTGAACAACTTGGTCCCCTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACCTCCCCCCAATCGGCTGCAACGCGGGCAATGGCACTCGACAAACACGGTACGATTCGCCTTATTCGAACCGGTAACTTTAGtatccgc                                     >  2:293314/1‑292 (MQ=255)                                                                                                                                                                                                                                                                        gTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTaa                       >  1:241092/1‑298 (MQ=255)                                                                                                                                                                                                                                                                              ttGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATACCGCCAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAACGTCGTCTGATTCCCTGTCCTCAGAACGGTAAATTTCGTTACGGCCTGCCcgggtgcagtttatc                 >  2:57429/1‑286 (MQ=255)                                                                                                                                                                                                                                                                                    aaaGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACCAGCaga           <  1:213671/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                     aaGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCATAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACCAGCagag          <  1:293314/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                           gTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACCAGCAGAGCCGaaa    >  1:316883/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                             acacGTGGCGGTGGGCGGTTAACAAGGTTCAGCCAGAACAGCCAGCGCAGGCAGAAATGCCGGAAGAAACAACCACGGCAACGGTCGCGAAAATTTCAGAGGGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTTGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACCAGCAGAGCCGAAAGc  <  2:316883/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                      ATGAAAACATTGCTCGGCGAGAGGATTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGTGCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACCTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACCAGCAGAGCCGAAAGC  >  CP006584/1459607‑1460188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑