| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006584 | 1,464,821 | A→G | intergenic (+486/‑118) | LY180_06955 → / → LY180_06960 | hypothetical protein/regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006584 | 1,464,821 | 0 | A | G | 94.4% | 56.0 / NA | 19 | intergenic (+486/‑118) | LY180_06955/LY180_06960 | hypothetical protein/regulatory protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (10/7); minor base T (0/1); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGTTGTGTTCCATTTCTACAGAACAAAATC‑ACAGAAACTATACCCAATAGTTGCATTGAATCACTGACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCAGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGC‑GACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCGTAGACTAC > CP006584/1464544‑1465106 | tttttgtTTTCCATTTTTAAAAAAAAAAATCGACA‑AAACTTATCCCAAAGGTTTCATTGAATTCATTACGGGACATCCTCATATTTATCAGGACTGGTTTACGTCCAATACCGGAGGGTGTCGTGCTGGTTCTCAAATGTGCGCTTGCTATTGCGGCTGTAATGGTAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCa < 2:230265/295‑1 (MQ=255) aCTATACCCAATAGTTGCATTGAATCACTGACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGc < 1:30198/298‑1 (MQ=255) gTTGCATTGAATCACTGACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACa > 1:88687/1‑298 (MQ=255) aaTCACTGACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGa > 1:46535/1‑298 (MQ=255) gACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCata < 1:68528/298‑1 (MQ=255) tGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGC‑GACACATAGAGATTAGCctct < 1:97954/298‑1 (MQ=255) aCAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGC‑GACACATAGAGATTAGCCTCTTATGTGCCGAAAGGc > 1:42027/1‑298 (MQ=255) ggTTGTCGTGCTGGCTCTCAAATGTGCTCTAGCTATTGCGGCTGTCATGGCACTATATTGTCTTGCTGTTGTTCTTAGGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTTACGGGGGTTAATAAGAATTGCAGCGCGTGCTGGAGGCAATCTGTCTCCGGCATGAAGACCATCAGGCAGATAGAGAAAAGACCCAGTTACTATTACGCATACGGCCAGACGCGTTACAAAACTCTCAGGCAAGAT‑TACACATACACATCGACACTATCCTCTTACGTGCCGAACGGAAACgaa > 2:256071/1‑297 (MQ=255) cTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGC‑GACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTa > 1:108338/1‑298 (MQ=255) agctaTTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGC‑GACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATg < 1:103715/298‑1 (MQ=255) aTTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGC‑GACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTaa > 1:239900/1‑298 (MQ=255) tCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGC‑GAcaca > 1:251487/1‑174 (MQ=255) tCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGC‑GAcaca < 2:251487/174‑1 (MQ=255) gattTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGC‑GACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGTAACGAGGAAAGACct > 1:203600/1‑298 (MQ=255) tatTCGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAACGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGCCGCTTAACATTAATCTGAGGCCATACCTATGC‑GACACATACAGCTTAGCCTCTTCATCGCCGAAAAGCAAGGGGAAGCAAGCTCTGAAGCAGCCAAAGGCGATGTTAAACGCCCTGCCCGAAATTTGTCAAACAGTCATTGTTATGACACTCGTACCGAGGAAAAACCACTCCGAg > 2:213055/1‑298 (MQ=18) ttAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGC‑GACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCCGAACCGGCCa > 1:117537/1‑298 (MQ=255) gTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGC‑GACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTc < 1:216533/298‑1 (MQ=255) tgattGGGGCTCTATGTCTCTGGCATGATCAACAACAGGCAGCTAGAAAAAAGCCCCAGGTAACATTATGCGGCCGAGAAGACGTTTAACATTAAACTGAGGCAATAGCTATGC‑GACACATAGAGATTAGCCTCTGATGTGCGGGAATGCAAGGAGAAGCAGACTATGAAGCAGCCAAAGGCGAGGTTAATCGCTCTGTTCGTCATCTGGTTAACCGTCTTTGTGACGGCACTGGTAACGAGGAAAGACATCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCg < 2:88687/295‑1 (MQ=255) gCTATCTGTCTCGGGCATGAACACCAAACGTCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGCGGCCATATCTATGC‑GACCCATCGAGATTAGCCCATTATGTGCCGAAAGGCAAGGAGAACCAGGCTATGAAGCAGCACAAGGCGATGTTAATCGCCCTACTCGTCATCTGTTTAACCGTCATTGTGACGGCAATGGTAACGCGAAAAGACATCTGCGACGTAATAATCCGCACCGGCCCAACGGAGTGCGCTTGCTTccgtaccctc > 2:40819/1‑289 (MQ=25) | TTGTTGTGTTCCATTTCTACAGAACAAAATC‑ACAGAAACTATACCCAATAGTTGCATTGAATCACTGACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCAGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGC‑GACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCGTAGACTAC > CP006584/1464544‑1465106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |