Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,505,880	T→C	L35L (CTA→CTG)	LY180_07200 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,505,880	0	T	C	92.9%	41.3 / ‑6.5	14	L35L (CTA→CTG)	LY180_07200	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (9/4);  total (10/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCCATCAGCAGGCTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAATAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGAATTACGTCGTTGGATTTATCTTCAAAGAATAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTGGCGTAACGGC  >  CP006584/1505607‑1506035                                                                                                                                                                                                                                                                                  |                                                                                                                                                            ccGCCATCAGCAGGCTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAgg                                                                                                                                     <  1:225681/298‑1 (MQ=255)                   gcgaAACGGGTGTGGTTACTTTCTTCATTGTGTTTGTCAATTACAGTTTCGGACGCAGGGTTATTTTAAGAATAGAGGATGAAAGGGTATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGTGAGCGGCAAGATTAAACAAGTTGGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATcc                                                                                                                   <  2:336600/298‑1 (MQ=255)                     gcAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGAAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTg                                                                                                                 >  1:89403/1‑298 (MQ=255)                           gtggcggcgATTTTCTGATTTGTGGTTTTCAGTTTAAGTTGCGGACGGAGGGCTATTTTAAGAGTAGATGATTGAAGGTCATTGTGGAGTATCTGAATCAGATCCCCTGGGTTTCCGGGGAGCGGCAAGATTAAACCAGTTCGTTCGAGCAGGTTTCGCCTGTTTCCATATTGCTTAAGTTGTGCAGCGTTGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTTCCCGGTAAACAGCACGTTGTGGCATGCAGACAGGCGACGGAATACGTCAGCCTGGATCAc                                                                                                           <  2:319146/295‑1 (MQ=255)                                      tttCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGAtt                                                                                                >  1:255872/1‑298 (MQ=255)                                                                        cTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATa                                                              >  1:157384/1‑298 (MQ=255)                                                                                      aaGACTAGAGGATGAAAGGTCATTGGGGATTATCTGAATCACCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAGCCAGTTCGTTCGGGCAGGTTTCGCCTTTGTCCAGATTGCTCAAGTTTTGCAGCGTAGTCTTAGAACTACTCGTCAGAGCTTCTGCTGTCAGGACTGCATGGTGCCCGGTAACTAGCAAGCTGTGGACGGCAGACAGGCGAGGGCATACGTCCTACGGGATCAGAGCGTTGGACTGATCTTAACAGAACAGATCGCTGTCGGTCGCACACACGTCAGTAccga                                                >  2:105404/1‑296 (MQ=255)                                                                                          aTAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGa                                            >  1:221946/1‑298 (MQ=255)                                                                                          aTAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGa                                            >  1:45424/1‑298 (MQ=255)                                                                                                    aaaGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTc                                  <  1:73444/298‑1 (MQ=255)                                                                                                                         gAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCCGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGACTACGTCATCCTGGATCAAGTCGTTGGATTTACCTTCAACGAAAATAACGCTTTCGTTCTCATCCACCTCACTCCCACACGACCCATATTTCTACTTTTTCCGCGCTCCCAtt             >  2:287954/1‑298 (MQ=255)                                                                                                                           aTCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTgc           >  1:168358/1‑297 (MQ=255)                                                                                                                              aGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTGTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACCGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTACAAAGAACAAATCGCGTTCGGTCTCAGACACGTCCATACCCAACGAACCAATTTACTCATTTTTCAgcacctcccttgttcc        >  2:280713/1‑284 (MQ=255)                                                                                                                                    ccTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTgctgcctgaga  >  1:179397/1‑288 (MQ=255)                                                                                                                                                                                                                                                                                  |                                                                                                                                                            CCGCCATCAGCAGGCTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAATAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGAATTACGTCGTTGGATTTATCTTCAAAGAATAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTGGCGTAACGGC  >  CP006584/1505607‑1506035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑