Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,505,925	A→G	I20I (ATT→ATC)	LY180_07200 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,505,925	0	A	G	100.0%	28.1 / NA	11	I20I (ATT→ATC)	LY180_07200	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/2);  total (9/2)

AGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAATAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGAATTACGTCGTTGGATTTATCTTCAAAGAATAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTGGCGTAACGGC  >  CP006584/1505633‑1506035                                                                                                                                                                                                                                                                                                     |                                                                                                               gtggcggcgATTTTCTGATTTGTGGTTTTCAGTTTAAGTTGCGGACGGAGGGCTATTTTAAGAGTAGATGATTGAAGGTCATTGTGGAGTATCTGAATCAGATCCCCTGGGTTTCCGGGGAGCGGCAAGATTAAACCAGTTCGTTCGAGCAGGTTTCGCCTGTTTCCATATTGCTTAAGTTGTGCAGCGTTGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTTCCCGGTAAACAGCACGTTGTGGCATGCAGACAGGCGACGGAATACGTCAGCCTGGATCAc                                                                                                           <  2:319146/295‑1 (MQ=255)            tttCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGAtt                                                                                                >  1:255872/1‑298 (MQ=255)                                              cTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATa                                                              >  1:157384/1‑298 (MQ=255)                                                            aaGACTAGAGGATGAAAGGTCATTGGGGATTATCTGAATCACCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAGCCAGTTCGTTCGGGCAGGTTTCGCCTTTGTCCAGATTGCTCAAGTTTTGCAGCGTAGTCTTAGAACTACTCGTCAGAGCTTCTGCTGTCAGGACTGCATGGTGCCCGGTAACTAGCAAGCTGTGGACGGCAGACAGGCGAGGGCATACGTCCTACGGGATCAGAGCGTTGGACTGATCTTAACAGAACAGATCGCTGTCGGTCGCACACACGTCAGTAccga                                                >  2:105404/1‑296 (MQ=255)                                                                aTAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGa                                            >  1:221946/1‑298 (MQ=255)                                                                aTAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGa                                            >  1:45424/1‑298 (MQ=255)                                                                          aaaGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTc                                  <  1:73444/298‑1 (MQ=255)                                                                                               gAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCCGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGACTACGTCATCCTGGATCAAGTCGTTGGATTTACCTTCAACGAAAATAACGCTTTCGTTCTCATCCACCTCACTCCCACACGACCCATATTTCTACTTTTTCCGCGCTCCCAtt             >  2:287954/1‑298 (MQ=255)                                                                                                 aTCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTgc           >  1:168358/1‑297 (MQ=255)                                                                                                    aGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTGTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACCGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTACAAAGAACAAATCGCGTTCGGTCTCAGACACGTCCATACCCAACGAACCAATTTACTCATTTTTCAgcacctcccttgttcc        >  2:280713/1‑284 (MQ=255)                                                                                                          ccTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTgctgcctgaga  >  1:179397/1‑288 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                               AGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAATAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGAATTACGTCGTTGGATTTATCTTCAAAGAATAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTGGCGTAACGGC  >  CP006584/1505633‑1506035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑